LOCUS       sequence-272495-        7103 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression of CENP-U CENP-Q CENP-OP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   McKinley KL, Sekulic N, Guo LY, Tsinman T, Black BE, Cheeseman IM
  TITLE     The CENP-L-N Complex Forms a Critical Node in an Integrated Meshwork
            of Interactions at the Centromere-Kinetochore Interface.
  JOURNAL   Mol Cell. 2015 Dec 17;60(6):886-98. doi: 
            10.1016/j.molcel.2015.10.027. Epub 2015 Nov 19.
  PUBMED    26698661
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.10.027"; journalName: "Mol Cell"; date: 
            "2015-12-17- 17"; volume: "60"; issue: "6"; pages: "886-98"
COMMENT     Sequence Label: pKM499
FEATURES             Location/Qualifiers
     source          1..7103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(41..46,47..63,64..69))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(140..173)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        250..341
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             387..407
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     polyA_signal    1877..2011
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2183..2274
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    3308..3442
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3614..3705
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    4799..4933
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  5054..5219
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      5409..5997
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5898..5917
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  6132..6355
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(6422..6955)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 tctggaccag ttgcgtgagc gcatacgcta cttgcattac agtttacgaa ccgaacaggc
       61 ttatgtcaac tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc cggcaacctt
      121 gggcagcagc gaagtcgcca taacttcgta tagcatacat tatacgaagt tatctgtaac
      181 tataacggtc ctaaggtagc gagtttaaac actagtatcg attcgcgacc tactccggaa
      241 tattaataga tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt
      301 actgttttcg taacagtttt gtaataaaaa aacctataaa tattccggat tattcatacc
      361 gtcccaccat cgggcgcgga tccatgcatc atcatcatca tcatcatggc agcggcagca
      421 tggccccgcg cggtcgtcgc cgtcctcgtc cgcaccgtag cgaaggtgcc cgtcgtagca
      481 agaacaccct ggagcgcacc cacagcatga aggacaaggc cggtcagaag tgcaaaccta
      541 tcgacgtgtt cgacttcccg gacaacagcg acgtgagtag tatcggccgc ctgggtgaga
      601 atgagaagga cgaggagacc tacgagacat tcgatccgcc tctgcatagc accgccattt
      661 acgcagacga agaggagttc agcaagcact gcggcctgag cttaagcagc accccgcctg
      721 gcaaggaagc aaagcgtagc agtgacacca gtggcaatga ggccagtgaa atcgagagcg
      781 tgaagatcag cgcaaagaag cctggccgta agctgcgccc gatcagcgac gatagcgaga
      841 gcattgagga gagtgatacc cgccgcaagg tgaaaagcgc agagaagatc agcacccagc
      901 gccacgaagt tattcgcacc acagcaagca gcgaactgag cgagaaaccg gccgaaagcg
      961 tgacaagtaa aaagacaggc ccgctgagcg cacaacctag cgtggaaaag gagaacctgg
     1021 ccatcgaaag ccagagtaag acccaaaaaa aaggtaagat tagccatgac aagcgcaaga
     1081 agagccgtag caaggccatc ggcagcgaca ccagtgacat cgtgcacatt tggtgcccgg
     1141 agggtatgaa aaccagcgac attaaagagc tgaacatcgt gctgccggag tttgagaaga
     1201 cccacctgga acaccagcag cgcatcgaga gtaaggtgtg caaggccgcc atcgccacct
     1261 tttacgttaa tgttaaagag cagtttatta aaatgctgaa ggagagccag atgctgacca
     1321 acctgaagcg caagaacgcc aagatgatca gcgacatcga gaagaagcgc cagcgcatga
     1381 tcgaggtgca ggacgaatta ctgcgcctgg agccgcagct gaagcaactg cagacaaagt
     1441 acgacgagct gaaggagcgc aagagcagcc tgcgcaacgc agcctacttt ctgagcaacc
     1501 tgaaacagtt atatcaagat tacagcgatg tgcaggcaca ggagccgaat gtgaaagaga
     1561 catacgacag cagtagtctg cctgccttac tgttcaaggc ccgtaccctg ttaggtgcag
     1621 agagccatct gcgcaacatc aaccaccagc tggaaaagtt actggaccag ggctaagaat
     1681 tcaaaggcct acgtcgacga gctcacttgt cgcggccgct ttcgaatcta gagcctgcag
     1741 tctcgacaag cttgtcgaga agtactagag gatcataatc agccatacca catttgtaga
     1801 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     1861 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     1921 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1981 actcatcaat gtatcttatc atgtctggat ctgatcactg cttgagccta gaagatccgg
     2041 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactat
     2101 cataacccct agggtatacc catctaaggt agcgagttta aacactagta tcgattcgcg
     2161 acctactccg gaatattaat agatcatgga gataattaaa atgataacca tctcgcaaat
     2221 aaataagtat tttactgttt tcgtaacagt tttgtaataa aaaaacctat aaatattccg
     2281 gattattcat accgtcccac catcgggcgc ggatccatga gcggcaaggc caacgccagc
     2341 aagaagaatg cccagcagct gaagcgcaat ccgaagcgta agaaggacaa cgaagaggtg
     2401 gtgctgagcg agaacaaggt gcgcaacacc gtgaagaaga ataaaaacca cctgaaagac
     2461 ctgagcagcg agggccagac caagcataca aacctgaagc acggcaagac cgccgccagc
     2521 aagcgtaaaa cctggcagcc tctgagcaag agcacccgtg accacctgca gaccatgatg
     2581 gagagcgtga tcatgaccat cctgagtaac agcatcaagg agaaagagga gatccagtat
     2641 catctgaact tcctgaagaa gcgtctgctg caacagtgcg agaccctgaa ggtgccgccg
     2701 aagaagatgg aggatctgac caacgtgagc agcctgctga acatggagcg cgcacgcgac
     2761 aaggcaaacg aagaggggct ggccctgctg caagaggaga tcgacaagat ggtggagacc
     2821 acagagctga tgaccggcaa catccaaagc ctgaagaaca agatccagat cctggccagc
     2881 gaggttgaag aagaagagga gcgcgtgaaa cagatgcacc agatcaacag cagcggcgtg
     2941 ctgagcttac cggaactgag ccagaaaacc ctgaaggcac cgaccctgca gaaggaaatc
     3001 ctggccctga ttccgaacca gaacgccctg ctgaaggacc tggacatcct gcacaacagc
     3061 agccagatga agagcatgag cacattcatc gaggaagcct acaagaagct ggacgccagt
     3121 taagtcgacg agctcacttg tcgcggccgc tttcgaatct agagcctgca gtctcgacaa
     3181 gcttgtcgag aagtactaga ggatcataat cagccatacc acatttgtag aggttttact
     3241 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     3301 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3361 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3421 tgtatcttat catgtctgga tctgatcact gcttgagcct agaagatccg gctgctaaca
     3481 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta tcataacccc
     3541 tagggtatac ccatctaagg tagcgagttt aaacactagt atcgattcgc gacctactcc
     3601 ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta
     3661 ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca
     3721 taccgtccca ccatcgggcg cggatccatg gatgccgaac tggctgaagt ccgtgctctg
     3781 caagctgaga tcgctgctct gcgtcgtgcc tgtgaagatc ctccggctcc ttgggaagaa
     3841 aaatcccgtg tccagaaatc atttcaggcc attcatcagt ttaacctgga ggggtggaaa
     3901 agctctaaag acctgaaaaa ccagctgggt catctggaat cagagctgtc gtttctgtct
     3961 accctgaccg gcattaacat tcgtaaccac tcaaaacaaa ccgaggacct gacctctaca
     4021 gagatgacgg agaaatccat ccgtaaagtg ctgcagcgcc atcgtctgtc tggaaattgc
     4081 cacatggtga cctttcaact ggagttccaa atcctggaga ttcagaacaa agaacgcctg
     4141 tctagtgctg ttactgatct gaacatcatc atggagccaa ccgaatgctc cgaactgagc
     4201 gaatttgtta gccgtgccga agagcgtaaa gacctgttca tgttttttcg ttcactgcat
     4261 ttttttgtgg aatggttcga gtatcgtaaa cgcaccttca aacatctgaa agagaaatat
     4321 cctgacgccg tgtatctgtc cgaaggccct tcaagttgta gcatgggtat ccgctcggcc
     4381 tctcgtccgg ggttcgaact ggttattgtg tggcgtattc aaatcgatga ggacggcaaa
     4441 gtgttcccga aactggatct gctgaccaaa gttcctcaac gtgccctgga gctggataaa
     4501 aatcgtgcca tcgaaaccgc tcctctgtct ttccgtaccc tggttggtct gctgggtatt
     4561 gaagcagccc tggaaagcct gattaaaagt ctgtgtgccg aggagaacaa ctgagtcgac
     4621 gagctcactt gtcgcggccg ctttcgaatc tagagcctgc agtctcgaca agcttgtcga
     4681 gaagtactag aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     4741 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     4801 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     4861 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     4921 tcatgtctgg atctgatcac tgcttgagcc tagaagatcc ggctgctaac aaagcccgaa
     4981 aggaagctga gttggctgct gccaccgctg agcaataact atcataaccc ctagggtata
     5041 cccatctaat tggaaccaga taagtgaaat ctagttccaa actattttgt catttttaat
     5101 tttcgtatta gcttacgacg ctacacccag ttcccatcta ttttgtcact cttccctaaa
     5161 taatccttaa aaactccatt tccacccctc ccagttccca actattttgt ccgcccacaa
     5221 ccggttgact tgggtcaact gtcagaccaa gtttactcat atatacttta gattgattta
     5281 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5341 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5401 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5461 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5521 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     5581 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     5641 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     5701 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     5761 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     5821 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     5881 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     5941 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6001 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     6061 tttcctgcgt tatcccctga ttgacttggg tcgctcttcc tgtggatgcg cagatgccct
     6121 gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga tctaaactat
     6181 gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc agttatgctg
     6241 tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt ctgaagtgca
     6301 aattgcccgt cgtattaaag aggggcgtgg ccaagggcat gtaaagacta tattcgcggc
     6361 gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc gatctcggct tgaacgaatt
     6421 gttaggtggc ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact
     6481 ttgtatagag agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag
     6541 caccaagcgc gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc
     6601 tccggtgctc gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa
     6661 acttgggcag aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa
     6721 gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt
     6781 gggagtaggt ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg
     6841 atttgacttg gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta
     6901 actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg
     6961 ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga
     7021 ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
     7081 ccaccgctgc gttcggtcaa ggt
//
