LOCUS       sequence-272496-        5573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SNR6 has UU at position 105–106 deleted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Toroney R, Nielsen KH, Staley JP
  TITLE     Termination of pre-mRNA splicing requires that the ATPase and RNA 
            unwindase Prp43p acts on the catalytic snRNA U6.
  JOURNAL   Genes Dev. 2019 Sep 26. pii: gad.328294.119. doi: 
            10.1101/gad.328294.119.
  PUBMED    31558568
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2019 Sep 26. pii: gad.328294.119. doi: 10.1101/gad.328294.119."
COMMENT     Sequence Label: pJPS2951
FEATURES             Location/Qualifiers
     source          1..5573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(669..686)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(669..685)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(678..700)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    1200..1492
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      1493..1946
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     CDS             complement(1785..2459)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(2460..2561)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(2578..2596)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2664..2768
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2769..2837,2838..3629)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2987..3006)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3800..4388
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4289..4308
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4542..4559
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4676..4697
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4712..4717,4718..4735,4736..4742)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4750..4766
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4755..4777
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4774..4790
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4774..4790
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gttcgcgaag taacccttcg tggacatttg gtcaatttga aacaatacag agatgatcag
       61 cagttcccct gcataaggat gaaccgtttt acaaagagat ttatcgtttt ttttttatcc
      121 tcgagtgaag tatcgtgact tgtacatttg aagataccca gcgtacagca gtgtatcttt
      181 atcttcctgt atgatataga taactaacat ctcgaataga aaattgtctc gcgttcgaac
      241 ctaagccttt gctatcggtt gaacatagaa gaccgcgtga ttcattttag tgcgtaagaa
      301 tgctattagt acttatgtgc tttatgaatg tgaatattgt tacagctatg caatggtacg
      361 atcatactct gaacgatcca tttaaattat atatgtcaaa cagaagagaa aggttttttt
      421 attgaagatt tcacagaaaa tcaatcattt tgcaatattc cttcgtccca gagaaggttt
      481 cggcttttac agcaacgaga atgattcttc cattatttgt acctagcttc cacatggatt
      541 tcgataagtg tagtttcaaa ccatgaacgc aattcaaaac tttcaaattg gtgcaatcca
      601 agaccctccc tttacatttg gagtcgttat caagcttatc gataccgtcg acggatccga
      661 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      721 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      781 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
      841 tttctcctta cgcatctgtg cggtatttca caccgcataa ctagctttta acttgtatcc
      901 taggttatct atgctgtctc accatagaga atattaccta tttcagaatg tatgtccatg
      961 attcgccggg taaatacata taatacacaa atctggctta ataaagtcta taatatatct
     1021 cataaagaag tgctaaattg gctagtgcta tatattttta agaaaatttc ttttgactaa
     1081 gtccatatcg actttgtaaa agttcacatt agcatacata tattacacga gccagaaata
     1141 gtaacttttg cctaaatcac aaattgcaaa atttaattgc ttgcaaaagg tcacatgctt
     1201 ataatcaact tttttaaaaa tttaaaatac ttttttattt tttattttta aacataaatg
     1261 aaataattta tttattgttt atgattaccg aaacataaaa cctgctcaag aaaaagaaac
     1321 tgttttgtcc ttggaaaaaa agcactacct aggagcggcc aaaatgccga ggctttcata
     1381 gcttaaactc tttacagaaa ataggcatta tagatcagtt cgagttttct tattcttcct
     1441 tccggtttta tcgtcacagt tttacagtaa ataagtatca cctcttagag ttggcggttg
     1501 tttgcaagac cgagaaaagg ctagcaagaa tcgggtcatt gtagcgtatg cgcctgtgaa
     1561 cattctcttc aacaagtttg attccattgc ggtgaaatgg taaaagtcaa ccccctgcga
     1621 tgtatatttt cctgtacaat caatcaaaaa gccaaatgat ttagcattat ctttacatct
     1681 tgttatttta cagattttat gtttagatct tttatgcttg cttttcaaaa ggcctgcagg
     1741 caagtgcaca aacaatactt aaataaatac tactcagtaa taacctattt cttagcattt
     1801 ttgacgaaat ttgctatttt gttagagtct tttacaccat ttgtctccac acctccgctt
     1861 acatcaacac caataacgcc atttaatcta agcgcatcac caacattttc tggcgtcagt
     1921 ccaccagcta acataaaatg taagctttcg gggctctctt gccttccaac ccagtcagaa
     1981 atcgagttcc aatccaaaag ttcacctgtc ccacctgctt ctgaatcaaa caagggaata
     2041 aacgaatgag gtttctgtga agctgcactg agtagtatgt tgcagtcttt tggaaatacg
     2101 agtcttttaa taactggcaa accgaggaac tcttggtatt cttgccacga ctcatctcca
     2161 tgcagttgga cgatatcaat gccgtaatca ttgaccagag ccaaaacatc ctccttaggt
     2221 tgattacgaa acacgccaac caagtatttc ggagtgcctg aactattttt atatgctttt
     2281 acaagacttg aaattttcct tgcaataacc gggtcaattg ttctctttct attgggcaca
     2341 catataatac ccagcaagtc agcatcggaa tctagagcac attctgcggc ctctgtgctc
     2401 tgcaagccgc aaactttcac caatggacca gaactacctg tgaaattaat aacagacata
     2461 ctccaagctg cctttgtgtg cttaatcacg tatactcacg tgctcaatag tcaccaatgc
     2521 cctccctctt ggccctctcc ttttcttttt tcgaccgaat ttcttgaaga cgaaagggcc
     2581 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     2641 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     2701 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     2761 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     2821 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     2881 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     2941 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     3001 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     3061 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     3121 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     3181 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     3241 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     3301 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     3361 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     3421 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     3481 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     3541 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     3601 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     3661 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     3721 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     3781 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3841 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3901 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     3961 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     4021 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     4081 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     4141 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     4201 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     4261 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     4321 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     4381 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     4441 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     4501 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     4561 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     4621 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     4681 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     4741 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga
     4801 attcgagctc ggtacccggg gatcccccgg gctgcaggaa ttcgatatcg tccattccga
     4861 cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc aatttctatg
     4921 cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc
     4981 gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc tgtggatcga
     5041 taattctcca taatagttct gttatttata atctccagca ctaataaatg ctatacgtat
     5101 atttgtacac aatataattt cagaatttat attgctacca tgactgtctg agaattgggg
     5161 gaataacttg ataattgttg ggattccatt gttcgtaaac gcaataatat taggtatata
     5221 gaagatacta aatgttctct ccgaggatat aggaatgctc acaatggaat cgatatattt
     5281 ctacataata gtattgagat tattcctctt ttagttttat ataattcatt atcctattac
     5341 attatcaatc cttgcatttc agcttccatt agacttaatg actgtttctc aatttttatg
     5401 tcatcttcct ggacctcatg tgatactata ccagtagcat gaatactact gaatcgatga
     5461 tactttagag tttcattgca acagtttcaa cacagcctgg catgaacagt ggtaaaagta
     5521 tttcgtccac tattttcggc tactataaat aaatgttttt ttcgcatgca tgt
//
