LOCUS       sequence-272499-        5572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SNR6 has UUC at position 105–107 deleted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Toroney R, Nielsen KH, Staley JP
  TITLE     Termination of pre-mRNA splicing requires that the ATPase and RNA 
            unwindase Prp43p acts on the catalytic snRNA U6.
  JOURNAL   Genes Dev. 2019 Sep 26. pii: gad.328294.119. doi: 
            10.1101/gad.328294.119.
  PUBMED    31558568
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2019 Sep 26. pii: gad.328294.119. doi: 10.1101/gad.328294.119."
COMMENT     Sequence Label: pJPS2952
FEATURES             Location/Qualifiers
     source          1..5572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(668..685)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(668..684)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(677..699)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    1199..1491
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      1492..1945
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     CDS             complement(1784..2458)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(2459..2560)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(2577..2595)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2663..2767
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2768..2836,2837..3628)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2986..3005)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3799..4387
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4288..4307
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4541..4558
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4675..4696
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4711..4716,4717..4734,4735..4741)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4749..4765
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4754..4776
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4773..4789
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4773..4789
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gttcgcgaag taacccttcg tggacatttg gtcaatttga aacaatacag agatgatcag
       61 cagttcccct gcataaggat gaaccgtttt acaaagagat ttatgttttt tttttatcct
      121 cgagtgaagt atcgtgactt gtacatttga agatacccag cgtacagcag tgtatcttta
      181 tcttcctgta tgatatagat aactaacatc tcgaatagaa aattgtctcg cgttcgaacc
      241 taagcctttg ctatcggttg aacatagaag accgcgtgat tcattttagt gcgtaagaat
      301 gctattagta cttatgtgct ttatgaatgt gaatattgtt acagctatgc aatggtacga
      361 tcatactctg aacgatccat ttaaattata tatgtcaaac agaagagaaa ggttttttta
      421 ttgaagattt cacagaaaat caatcatttt gcaatattcc ttcgtcccag agaaggtttc
      481 ggcttttaca gcaacgagaa tgattcttcc attatttgta cctagcttcc acatggattt
      541 cgataagtgt agtttcaaac catgaacgca attcaaaact ttcaaattgg tgcaatccaa
      601 gaccctccct ttacatttgg agtcgttatc aagcttatcg ataccgtcga cggatccgaa
      661 gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
      721 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
      781 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt
      841 ttctccttac gcatctgtgc ggtatttcac accgcataac tagcttttaa cttgtatcct
      901 aggttatcta tgctgtctca ccatagagaa tattacctat ttcagaatgt atgtccatga
      961 ttcgccgggt aaatacatat aatacacaaa tctggcttaa taaagtctat aatatatctc
     1021 ataaagaagt gctaaattgg ctagtgctat atatttttaa gaaaatttct tttgactaag
     1081 tccatatcga ctttgtaaaa gttcacatta gcatacatat attacacgag ccagaaatag
     1141 taacttttgc ctaaatcaca aattgcaaaa tttaattgct tgcaaaaggt cacatgctta
     1201 taatcaactt ttttaaaaat ttaaaatact tttttatttt ttatttttaa acataaatga
     1261 aataatttat ttattgttta tgattaccga aacataaaac ctgctcaaga aaaagaaact
     1321 gttttgtcct tggaaaaaaa gcactaccta ggagcggcca aaatgccgag gctttcatag
     1381 cttaaactct ttacagaaaa taggcattat agatcagttc gagttttctt attcttcctt
     1441 ccggttttat cgtcacagtt ttacagtaaa taagtatcac ctcttagagt tggcggttgt
     1501 ttgcaagacc gagaaaaggc tagcaagaat cgggtcattg tagcgtatgc gcctgtgaac
     1561 attctcttca acaagtttga ttccattgcg gtgaaatggt aaaagtcaac cccctgcgat
     1621 gtatattttc ctgtacaatc aatcaaaaag ccaaatgatt tagcattatc tttacatctt
     1681 gttattttac agattttatg tttagatctt ttatgcttgc ttttcaaaag gcctgcaggc
     1741 aagtgcacaa acaatactta aataaatact actcagtaat aacctatttc ttagcatttt
     1801 tgacgaaatt tgctattttg ttagagtctt ttacaccatt tgtctccaca cctccgctta
     1861 catcaacacc aataacgcca tttaatctaa gcgcatcacc aacattttct ggcgtcagtc
     1921 caccagctaa cataaaatgt aagctttcgg ggctctcttg ccttccaacc cagtcagaaa
     1981 tcgagttcca atccaaaagt tcacctgtcc cacctgcttc tgaatcaaac aagggaataa
     2041 acgaatgagg tttctgtgaa gctgcactga gtagtatgtt gcagtctttt ggaaatacga
     2101 gtcttttaat aactggcaaa ccgaggaact cttggtattc ttgccacgac tcatctccat
     2161 gcagttggac gatatcaatg ccgtaatcat tgaccagagc caaaacatcc tccttaggtt
     2221 gattacgaaa cacgccaacc aagtatttcg gagtgcctga actattttta tatgctttta
     2281 caagacttga aattttcctt gcaataaccg ggtcaattgt tctctttcta ttgggcacac
     2341 atataatacc cagcaagtca gcatcggaat ctagagcaca ttctgcggcc tctgtgctct
     2401 gcaagccgca aactttcacc aatggaccag aactacctgt gaaattaata acagacatac
     2461 tccaagctgc ctttgtgtgc ttaatcacgt atactcacgt gctcaatagt caccaatgcc
     2521 ctccctcttg gccctctcct tttctttttt cgaccgaatt tcttgaagac gaaagggcct
     2581 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     2641 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     2701 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     2761 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     2821 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     2881 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     2941 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     3001 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     3061 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     3121 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     3181 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     3241 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     3301 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     3361 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     3421 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     3481 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     3541 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     3601 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     3661 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     3721 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     3781 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3841 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3901 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     3961 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     4021 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     4081 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     4141 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     4201 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     4261 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     4321 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     4381 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     4441 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     4501 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     4561 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     4621 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     4681 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     4741 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
     4801 ttcgagctcg gtacccgggg atcccccggg ctgcaggaat tcgatatcgt ccattccgac
     4861 agcatcgcca gtcactatgg cgtgctgcta gcgctatatg cgttgatgca atttctatgc
     4921 gcacccgttc tcggagcact gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg
     4981 ctacttggag ccactatcga ctacgcgatc atggcgacca cacccgtcct gtggatcgat
     5041 aattctccat aatagttctg ttatttataa tctccagcac taataaatgc tatacgtata
     5101 tttgtacaca atataatttc agaatttata ttgctaccat gactgtctga gaattggggg
     5161 aataacttga taattgttgg gattccattg ttcgtaaacg caataatatt aggtatatag
     5221 aagatactaa atgttctctc cgaggatata ggaatgctca caatggaatc gatatatttc
     5281 tacataatag tattgagatt attcctcttt tagttttata taattcatta tcctattaca
     5341 ttatcaatcc ttgcatttca gcttccatta gacttaatga ctgtttctca atttttatgt
     5401 catcttcctg gacctcatgt gatactatac cagtagcatg aatactactg aatcgatgat
     5461 actttagagt ttcattgcaa cagtttcaac acagcctggc atgaacagtg gtaaaagtat
     5521 ttcgtccact attttcggct actataaata aatgtttttt tcgcatgcat gt
//
