LOCUS       sequence-272500-        5570 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SNR6 has AUUUC at position 103–107 deleted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Toroney R, Nielsen KH, Staley JP
  TITLE     Termination of pre-mRNA splicing requires that the ATPase and RNA 
            unwindase Prp43p acts on the catalytic snRNA U6.
  JOURNAL   Genes Dev. 2019 Sep 26. pii: gad.328294.119. doi: 
            10.1101/gad.328294.119.
  PUBMED    31558568
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2019 Sep 26. pii: gad.328294.119. doi: 10.1101/gad.328294.119."
COMMENT     Sequence Label: pJPS2953
FEATURES             Location/Qualifiers
     source          1..5570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(666..683)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(666..682)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(675..697)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    1197..1489
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      1490..1943
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     CDS             complement(1782..2456)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(2457..2558)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(2575..2593)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2661..2765
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2766..2834,2835..3626)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2984..3003)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3797..4385
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4286..4305
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4539..4556
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4673..4694
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4709..4714,4715..4732,4733..4739)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4747..4763
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4752..4774
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4771..4787
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4771..4787
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gttcgcgaag taacccttcg tggacatttg gtcaatttga aacaatacag agatgatcag
       61 cagttcccct gcataaggat gaaccgtttt acaaagagat ttgttttttt tttatcctcg
      121 agtgaagtat cgtgacttgt acatttgaag atacccagcg tacagcagtg tatctttatc
      181 ttcctgtatg atatagataa ctaacatctc gaatagaaaa ttgtctcgcg ttcgaaccta
      241 agcctttgct atcggttgaa catagaagac cgcgtgattc attttagtgc gtaagaatgc
      301 tattagtact tatgtgcttt atgaatgtga atattgttac agctatgcaa tggtacgatc
      361 atactctgaa cgatccattt aaattatata tgtcaaacag aagagaaagg tttttttatt
      421 gaagatttca cagaaaatca atcattttgc aatattcctt cgtcccagag aaggtttcgg
      481 cttttacagc aacgagaatg attcttccat tatttgtacc tagcttccac atggatttcg
      541 ataagtgtag tttcaaacca tgaacgcaat tcaaaacttt caaattggtg caatccaaga
      601 ccctcccttt acatttggag tcgttatcaa gcttatcgat accgtcgacg gatccgaagc
      661 ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
      721 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
      781 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
      841 ctccttacgc atctgtgcgg tatttcacac cgcataacta gcttttaact tgtatcctag
      901 gttatctatg ctgtctcacc atagagaata ttacctattt cagaatgtat gtccatgatt
      961 cgccgggtaa atacatataa tacacaaatc tggcttaata aagtctataa tatatctcat
     1021 aaagaagtgc taaattggct agtgctatat atttttaaga aaatttcttt tgactaagtc
     1081 catatcgact ttgtaaaagt tcacattagc atacatatat tacacgagcc agaaatagta
     1141 acttttgcct aaatcacaaa ttgcaaaatt taattgcttg caaaaggtca catgcttata
     1201 atcaactttt ttaaaaattt aaaatacttt tttatttttt atttttaaac ataaatgaaa
     1261 taatttattt attgtttatg attaccgaaa cataaaacct gctcaagaaa aagaaactgt
     1321 tttgtccttg gaaaaaaagc actacctagg agcggccaaa atgccgaggc tttcatagct
     1381 taaactcttt acagaaaata ggcattatag atcagttcga gttttcttat tcttccttcc
     1441 ggttttatcg tcacagtttt acagtaaata agtatcacct cttagagttg gcggttgttt
     1501 gcaagaccga gaaaaggcta gcaagaatcg ggtcattgta gcgtatgcgc ctgtgaacat
     1561 tctcttcaac aagtttgatt ccattgcggt gaaatggtaa aagtcaaccc cctgcgatgt
     1621 atattttcct gtacaatcaa tcaaaaagcc aaatgattta gcattatctt tacatcttgt
     1681 tattttacag attttatgtt tagatctttt atgcttgctt ttcaaaaggc ctgcaggcaa
     1741 gtgcacaaac aatacttaaa taaatactac tcagtaataa cctatttctt agcatttttg
     1801 acgaaatttg ctattttgtt agagtctttt acaccatttg tctccacacc tccgcttaca
     1861 tcaacaccaa taacgccatt taatctaagc gcatcaccaa cattttctgg cgtcagtcca
     1921 ccagctaaca taaaatgtaa gctttcgggg ctctcttgcc ttccaaccca gtcagaaatc
     1981 gagttccaat ccaaaagttc acctgtccca cctgcttctg aatcaaacaa gggaataaac
     2041 gaatgaggtt tctgtgaagc tgcactgagt agtatgttgc agtcttttgg aaatacgagt
     2101 cttttaataa ctggcaaacc gaggaactct tggtattctt gccacgactc atctccatgc
     2161 agttggacga tatcaatgcc gtaatcattg accagagcca aaacatcctc cttaggttga
     2221 ttacgaaaca cgccaaccaa gtatttcgga gtgcctgaac tatttttata tgcttttaca
     2281 agacttgaaa ttttccttgc aataaccggg tcaattgttc tctttctatt gggcacacat
     2341 ataataccca gcaagtcagc atcggaatct agagcacatt ctgcggcctc tgtgctctgc
     2401 aagccgcaaa ctttcaccaa tggaccagaa ctacctgtga aattaataac agacatactc
     2461 caagctgcct ttgtgtgctt aatcacgtat actcacgtgc tcaatagtca ccaatgccct
     2521 ccctcttggc cctctccttt tcttttttcg accgaatttc ttgaagacga aagggcctcg
     2581 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     2641 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     2701 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     2761 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     2821 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     2881 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     2941 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     3001 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     3061 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     3121 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     3181 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     3241 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     3301 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     3361 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     3421 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     3481 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     3541 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     3601 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     3661 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     3721 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     3781 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3841 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3901 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3961 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4021 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4081 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4141 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4201 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4261 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4321 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4381 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4441 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     4501 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     4561 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     4621 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     4681 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     4741 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt
     4801 cgagctcggt acccggggat cccccgggct gcaggaattc gatatcgtcc attccgacag
     4861 catcgccagt cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc
     4921 acccgttctc ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct
     4981 acttggagcc actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggatcgataa
     5041 ttctccataa tagttctgtt atttataatc tccagcacta ataaatgcta tacgtatatt
     5101 tgtacacaat ataatttcag aatttatatt gctaccatga ctgtctgaga attgggggaa
     5161 taacttgata attgttggga ttccattgtt cgtaaacgca ataatattag gtatatagaa
     5221 gatactaaat gttctctccg aggatatagg aatgctcaca atggaatcga tatatttcta
     5281 cataatagta ttgagattat tcctctttta gttttatata attcattatc ctattacatt
     5341 atcaatcctt gcatttcagc ttccattaga cttaatgact gtttctcaat ttttatgtca
     5401 tcttcctgga cctcatgtga tactatacca gtagcatgaa tactactgaa tcgatgatac
     5461 tttagagttt cattgcaaca gtttcaacac agcctggcat gaacagtggt aaaagtattt
     5521 cgtccactat tttcggctac tataaataaa tgtttttttc gcatgcatgt
//
