LOCUS       sequence-272502-        5569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SNR6 has UAUUUCat position 102–107 deleted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Toroney R, Nielsen KH, Staley JP
  TITLE     Termination of pre-mRNA splicing requires that the ATPase and RNA 
            unwindase Prp43p acts on the catalytic snRNA U6.
  JOURNAL   Genes Dev. 2019 Sep 26. pii: gad.328294.119. doi: 
            10.1101/gad.328294.119.
  PUBMED    31558568
REFERENCE   2  (bases 1 to 5569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2019 Sep 26. pii: gad.328294.119. doi: 10.1101/gad.328294.119."
COMMENT     Sequence Label: pJPS2954
FEATURES             Location/Qualifiers
     source          1..5569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(665..682)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(665..681)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(674..696)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    1196..1488
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      1489..1942
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     CDS             complement(1781..2455)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(2456..2557)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(2574..2592)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2660..2764
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2765..2833,2834..3625)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2983..3002)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3796..4384
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4285..4304
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4538..4555
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4672..4693
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4708..4713,4714..4731,4732..4738)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4746..4762
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4751..4773
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4770..4786
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4770..4786
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gttcgcgaag taacccttcg tggacatttg gtcaatttga aacaatacag agatgatcag
       61 cagttcccct gcataaggat gaaccgtttt acaaagagat tgtttttttt ttatcctcga
      121 gtgaagtatc gtgacttgta catttgaaga tacccagcgt acagcagtgt atctttatct
      181 tcctgtatga tatagataac taacatctcg aatagaaaat tgtctcgcgt tcgaacctaa
      241 gcctttgcta tcggttgaac atagaagacc gcgtgattca ttttagtgcg taagaatgct
      301 attagtactt atgtgcttta tgaatgtgaa tattgttaca gctatgcaat ggtacgatca
      361 tactctgaac gatccattta aattatatat gtcaaacaga agagaaaggt ttttttattg
      421 aagatttcac agaaaatcaa tcattttgca atattccttc gtcccagaga aggtttcggc
      481 ttttacagca acgagaatga ttcttccatt atttgtacct agcttccaca tggatttcga
      541 taagtgtagt ttcaaaccat gaacgcaatt caaaactttc aaattggtgc aatccaagac
      601 cctcccttta catttggagt cgttatcaag cttatcgata ccgtcgacgg atccgaagct
      661 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
      721 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      781 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
      841 tccttacgca tctgtgcggt atttcacacc gcataactag cttttaactt gtatcctagg
      901 ttatctatgc tgtctcacca tagagaatat tacctatttc agaatgtatg tccatgattc
      961 gccgggtaaa tacatataat acacaaatct ggcttaataa agtctataat atatctcata
     1021 aagaagtgct aaattggcta gtgctatata tttttaagaa aatttctttt gactaagtcc
     1081 atatcgactt tgtaaaagtt cacattagca tacatatatt acacgagcca gaaatagtaa
     1141 cttttgccta aatcacaaat tgcaaaattt aattgcttgc aaaaggtcac atgcttataa
     1201 tcaactyttt taaaaattta aaatactttt ttatttttta tttttaaaca taaatgaaat
     1261 aatttattta ttgtttatga ttaccgaaac ataaaacctg ctcaagaaaa agaaactgtt
     1321 ttgtccttgg aaaaaaagca ctacctagga gcggccaaaa tgccgaggct ttcatagctt
     1381 aaactcttta cagaaaatag gcattataga tcagttcgag ttttcttatt cttccttccg
     1441 gttttatcgt cacagtttta cagtaaataa gtatcacctc ttagagttgg cggttgtttg
     1501 caagaccgag aaaaggctag caagaatcgg gtcattgtag cgtatgcgcc tgtgaacatt
     1561 ctcttcaaca agtttgattc cattgcggtg aaatggtaaa agtcaacccc ctgcgatgta
     1621 tattttcctg tacaatcaat caaaaagcca aatgatttag cattatcttt acatcttgtt
     1681 attttacaga ttttatgttt agatctttta tgcttgcttt tcaaaaggcc tgcaggcaag
     1741 tgcacaaaca atacttaaat aaatactact cagtaataac ctatttctta gcatttttga
     1801 cgaaatttgc tattttgtta gagtctttta caccatttgt ctccacacct ccgcttacat
     1861 caacaccaat aacgccattt aatctaagcg catcaccaac attttctggc gtcagtccac
     1921 cagctaacat aaaatgtaag ctttcggggc tctcttgcct tccaacccag tcagaaatcg
     1981 agttccaatc caaaagttca cctgtcccac ctgcttctga atcaaacaag ggaataaacg
     2041 aatgaggttt ctgtgaagct gcactgagta gtatgttgca gtcttttgga aatacgagtc
     2101 ttttaataac tggcaaaccg aggaactctt ggtattcttg ccacgactca tctccatgca
     2161 gttggacgat atcaatgccg taatcattga ccagagccaa aacatcctcc ttaggttgat
     2221 tacgaaacac gccaaccaag tatttcggag tgcctgaact atttttatat gcttttacaa
     2281 gacttgaaat tttccttgca ataaccgggt caattgttct ctttctattg ggcacacata
     2341 taatacccag caagtcagca tcggaatcta gagcacattc tgcggcctct gtgctctgca
     2401 agccgcaaac tttcaccaat ggaccagaac tacctgtgaa attaataaca gacatactcc
     2461 aagctgcctt tgtgtgctta atcacgtata ctcacgtgct caatagtcac caatgccctc
     2521 cctcttggcc ctctcctttt cttttttcga ccgaatttct tgaagacgaa agggcctcgt
     2581 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     2641 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     2701 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     2761 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     2821 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     2881 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     2941 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     3001 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     3061 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     3121 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     3181 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     3241 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     3301 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     3361 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     3421 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     3481 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     3541 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     3601 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     3661 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     3721 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     3781 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3841 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3901 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     3961 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4021 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4081 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4141 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4201 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4261 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4321 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4381 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4441 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     4501 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     4561 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     4621 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     4681 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     4741 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattc
     4801 gagctcggta cccggggatc ccccgggctg caggaattcg atatcgtcca ttccgacagc
     4861 atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt tctatgcgca
     4921 cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta
     4981 cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg gatcgataat
     5041 tctccataat agttctgtta tttataatct ccagcactaa taaatgctat acgtatattt
     5101 gtacacaata taatttcaga atttatattg ctaccatgac tgtctgagaa ttgggggaat
     5161 aacttgataa ttgttgggat tccattgttc gtaaacgcaa taatattagg tatatagaag
     5221 atactaaatg ttctctccga ggatatagga atgctcacaa tggaatcgat atatttctac
     5281 ataatagtat tgagattatt cctcttttag ttttatataa ttcattatcc tattacatta
     5341 tcaatccttg catttcagct tccattagac ttaatgactg tttctcaatt tttatgtcat
     5401 cttcctggac ctcatgtgat actataccag tagcatgaat actactgaat cgatgatact
     5461 ttagagtttc attgcaacag tttcaacaca gcctggcatg aacagtggta aaagtatttc
     5521 gtccactatt ttcggctact ataaataaat gtttttttcg catgcatgt
//
