LOCUS       sequence-272504-        5567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SNR6 has UUUAUUUC at position 100–107 deleted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Toroney R, Nielsen KH, Staley JP
  TITLE     Termination of pre-mRNA splicing requires that the ATPase and RNA 
            unwindase Prp43p acts on the catalytic snRNA U6.
  JOURNAL   Genes Dev. 2019 Sep 26. pii: gad.328294.119. doi: 
            10.1101/gad.328294.119.
  PUBMED    31558568
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2019 Sep 26. pii: gad.328294.119. doi: 10.1101/gad.328294.119."
COMMENT     Sequence Label: pJPS2955
FEATURES             Location/Qualifiers
     source          1..5567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(663..680)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(663..679)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(672..694)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    1194..1486
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      1487..1940
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     CDS             complement(1779..2453)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(2454..2555)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(2572..2590)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2658..2762
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2763..2831,2832..3623)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2981..3000)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3794..4382
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4283..4302
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4536..4553
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4670..4691
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4706..4711,4712..4729,4730..4736)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4744..4760
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4749..4771
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4768..4784
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4768..4784
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gttcgcgaag taacccttcg tggacatttg gtcaatttga aacaatacag agatgatcag
       61 cagttcccct gcataaggat gaaccgtttt acaaagagag tttttttttt atcctcgagt
      121 gaagtatcgt gacttgtaca tttgaagata cccagcgtac agcagtgtat ctttatcttc
      181 ctgtatgata tagataacta acatctcgaa tagaaaattg tctcgcgttc gaacctaagc
      241 ctttgctatc ggttgaacat agaagaccgc gtgattcatt ttagtgcgta agaatgctat
      301 tagtacttat gtgctttatg aatgtgaata ttgttacagc tatgcaatgg tacgatcata
      361 ctctgaacga tccatttaaa ttatatatgt caaacagaag agaaaggttt ttttattgaa
      421 gatttcacag aaaatcaatc attttgcaat attccttcgt cccagagaag gtttcggctt
      481 ttacagcaac gagaatgatt cttccattat ttgtacctag cttccacatg gatttcgata
      541 agtgtagttt caaaccatga acgcaattca aaactttcaa attggtgcaa tccaagaccc
      601 tccctttaca tttggagtcg ttatcaagct tatcgatacc gtcgacggat ccgaagcttg
      661 gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
      721 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
      781 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
      841 cttacgcatc tgtgcggtat ttcacaccgc ataactagct tttaacttgt atcctaggtt
      901 atctatgctg tctcaccata gagaatatta cctatttcag aatgtatgtc catgattcgc
      961 cgggtaaata catataatac acaaatctgg cttaataaag tctataatat atctcataaa
     1021 gaagtgctaa attggctagt gctatatatt tttaagaaaa tttcttttga ctaagtccat
     1081 atcgactttg taaaagttca cattagcata catatattac acgagccaga aatagtaact
     1141 tttgcctaaa tcacaaattg caaaatttaa ttgcttgcaa aaggtcacat gcttataatc
     1201 aactttttta aaaatttaaa atactttttt attttttatt tttaaacata aatgaaataa
     1261 tttatttatt gtttatgatt accgaaacat aaaacctgct caagaaaaag aaactgtttt
     1321 gtccttggaa aaaaagcact acctaggagc ggccaaaatg ccgaggcttt catagcttaa
     1381 actctttaca gaaaataggc attatagatc agttcgagtt ttcttattct tccttccggt
     1441 tttatcgtca cagttttaca gtaaataagt atcacctctt agagttggcg gttgtttgca
     1501 agaccgagaa aaggctagca agaatcgggt cattgtagcg tatgcgcctg tgaacattct
     1561 cttcaacaag tttgattcca ttgcggtgaa atggtaaaag tcaaccccct gcgatgtata
     1621 ttttcctgta caatcaatca aaaagccaaa tgatttagca ttatctttac atcttgttat
     1681 tttacagatt ttatgtttag atcttttatg cttgcttttc aaaaggcctg caggcaagtg
     1741 cacaaacaat acttaaataa atactactca gtaataacct atttcttagc atttttgacg
     1801 aaatttgcta ttttgttaga gtcttttaca ccatttgtct ccacacctcc gcttacatca
     1861 acaccaataa cgccatttaa tctaagcgca tcaccaacat tttctggcgt cagtccacca
     1921 gctaacataa aatgtaagct ttcggggctc tcttgccttc caacccagtc agaaatcgag
     1981 ttccaatcca aaagttcacc tgtcccacct gcttctgaat caaacaaggg aataaacgaa
     2041 tgaggtttct gtgaagctgc actgagtagt atgttgcagt cttttggaaa tacgagtctt
     2101 ttaataactg gcaaaccgag gaactcttgg tattcttgcc acgactcatc tccatgcagt
     2161 tggacgatat caatgccgta atcattgacc agagccaaaa catcctcctt aggttgatta
     2221 cgaaacacgc caaccaagta tttcggagtg cctgaactat ttttatatgc ttttacaaga
     2281 cttgaaattt tccttgcaat aaccgggtca attgttctct ttctattggg cacacatata
     2341 atacccagca agtcagcatc ggaatctaga gcacattctg cggcctctgt gctctgcaag
     2401 ccgcaaactt tcaccaatgg accagaacta cctgtgaaat taataacaga catactccaa
     2461 gctgcctttg tgtgcttaat cacgtatact cacgtgctca atagtcacca atgccctccc
     2521 tcttggccct ctccttttct tttttcgacc gaatttcttg aagacgaaag ggcctcgtga
     2581 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     2641 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     2701 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     2761 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     2821 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     2881 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     2941 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     3001 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     3061 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     3121 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     3181 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     3241 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     3301 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     3361 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     3421 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     3481 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     3541 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     3601 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     3661 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     3721 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     3781 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3841 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3901 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     3961 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4021 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4081 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4141 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4201 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4261 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4321 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4381 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4441 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     4501 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     4561 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     4621 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     4681 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     4741 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaattcga
     4801 gctcggtacc cggggatccc ccgggctgca ggaattcgat atcgtccatt ccgacagcat
     4861 cgccagtcac tatggcgtgc tgctagcgct atatgcgttg atgcaatttc tatgcgcacc
     4921 cgttctcgga gcactgtccg accgctttgg ccgccgccca gtcctgctcg cttcgctact
     4981 tggagccact atcgactacg cgatcatggc gaccacaccc gtcctgtgga tcgataattc
     5041 tccataatag ttctgttatt tataatctcc agcactaata aatgctatac gtatatttgt
     5101 acacaatata atttcagaat ttatattgct accatgactg tctgagaatt gggggaataa
     5161 cttgataatt gttgggattc cattgttcgt aaacgcaata atattaggta tatagaagat
     5221 actaaatgtt ctctccgagg atataggaat gctcacaatg gaatcgatat atttctacat
     5281 aatagtattg agattattcc tcttttagtt ttatataatt cattatccta ttacattatc
     5341 aatccttgca tttcagcttc cattagactt aatgactgtt tctcaatttt tatgtcatct
     5401 tcctggacct catgtgatac tataccagta gcatgaatac tactgaatcg atgatacttt
     5461 agagtttcat tgcaacagtt tcaacacagc ctggcatgaa cagtggtaaa agtatttcgt
     5521 ccactatttt cggctactat aaataaatgt ttttttcgca tgcatgt
//
