LOCUS       sequence-272506-        5563 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SNR6 has GAGAUUUAUUUC at position 96–107 deleted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Toroney R, Nielsen KH, Staley JP
  TITLE     Termination of pre-mRNA splicing requires that the ATPase and RNA 
            unwindase Prp43p acts on the catalytic snRNA U6.
  JOURNAL   Genes Dev. 2019 Sep 26. pii: gad.328294.119. doi: 
            10.1101/gad.328294.119.
  PUBMED    31558568
REFERENCE   2  (bases 1 to 5563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2019 Sep 26. pii: gad.328294.119. doi: 10.1101/gad.328294.119."
COMMENT     Sequence Label: pJPS2957
FEATURES             Location/Qualifiers
     source          1..5563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(659..676)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(659..675)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(668..690)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    1190..1482
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      1483..1936
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     CDS             complement(1775..2449)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(2450..2551)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(2568..2586)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2654..2758
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2759..2827,2828..3619)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2977..2996)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3790..4378
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4279..4298
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4532..4549
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4666..4687
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4702..4707,4708..4725,4726..4732)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4740..4756
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4745..4767
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4764..4780
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4764..4780
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gttcgcgaag taacccttcg tggacatttg gtcaatttga aacaatacag agatgatcag
       61 cagttcccct gcataaggat gaaccgtttt acaaagtttt ttttttatcc tcgagtgaag
      121 tatcgtgact tgtacatttg aagataccca gcgtacagca gtgtatcttt atcttcctgt
      181 atgatataga taactaacat ctcgaataga aaattgtctc gcgttcgaac ctaagccttt
      241 gctatcggtt gaacatagaa gaccgcgtga ttcattttag tgcgtaagaa tgctattagt
      301 acttatgtgc tttatgaatg tgaatattgt tacagctatg caatggtacg atcatactct
      361 gaacgatcca tttaaattat atatgtcaaa cagaagagaa aggttttttt attgaagatt
      421 tcacagaaaa tcaatcattt tgcaatattc cttcgtccca gagaaggttt cggcttttac
      481 agcaacgaga atgattcttc cattatttgt acctagcttc cacatggatt tcgataagtg
      541 tagtttcaaa ccatgaacgc aattcaaaac tttcaaattg gtgcaatcca agaccctccc
      601 tttacatttg gagtcgttat caagcttatc gataccgtcg acggatccga agcttggcac
      661 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
      721 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
      781 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
      841 cgcatctgtg cggtatttca caccgcataa ctagctttta acttgtatcc taggttatct
      901 atgctgtctc accatagaga atattaccta tttcagaatg tatgtccatg attcgccggg
      961 taaatacata taatacacaa atctggctta ataaagtcta taatatatct cataaagaag
     1021 tgctaaattg gctagtgcta tatattttta agaaaatttc ttttgactaa gtccatatcg
     1081 actttgtaaa agttcacatt agcatacata tattacacga gccagaaata gtaacttttg
     1141 cctaaatcac aaattgcaaa atttaattgc ttgcaaaagg tcacatgctt ataatcaact
     1201 tttttaaaaa tttaaaatac ttttttattt tttattttta aacataaatg aaataattta
     1261 tttattgttt atgattaccg aaacataaaa cctgctcaag aaaaagaaac tgttttgtcc
     1321 ttggaaaaaa agcactacct aggagcggcc aaaatgccga ggctttcata gcttaaactc
     1381 tttacagaaa ataggcatta tagatcagtt cgagttttct tattcttcct tccggtttta
     1441 tcgtcacagt tttacagtaa ataagtatca cctcttagag ttggcggttg tttgcaagac
     1501 cgagaaaagg ctagcaagaa tcgggtcatt gtagcgtatg cgcctgtgaa cattctcttc
     1561 aacaagtttg attccattgc ggtgaaatgg taaaagtcaa ccccctgcga tgtatatttt
     1621 cctgtacaat caatcaaaaa gccaaatgat ttagcattat ctttacatct tgttatttta
     1681 cagattttat gtttagatct tttatgcttg cttttcaaaa ggcctgcagg caagtgcaca
     1741 aacaatactt aaataaatac tactcagtaa taacctattt cttagcattt ttgacgaaat
     1801 ttgctatttt gttagagtct tttacaccat ttgtctccac acctccgctt acatcaacac
     1861 caataacgcc atttaatcta agcgcatcac caacattttc tggcgtcagt ccaccagcta
     1921 acataaaatg taagctttcg gggctctctt gccttccaac ccagtcagaa atcgagttcc
     1981 aatccaaaag ttcacctgtc ccacctgctt ctgaatcaaa caagggaata aacgaatgag
     2041 gtttctgtga agctgcactg agtagtatgt tgcagtcttt tggaaatacg agtcttttaa
     2101 taactggcaa accgaggaac tcttggtatt cttgccacga ctcatctcca tgcagttgga
     2161 cgatatcaat gccgtaatca ttgaccagag ccaaaacatc ctccttaggt tgattacgaa
     2221 acacgccaac caagtatttc ggagtgcctg aactattttt atatgctttt acaagacttg
     2281 aaattttcct tgcaataacc gggtcaattg ttctctttct attgggcaca catataatac
     2341 ccagcaagtc agcatcggaa tctagagcac attctgcggc ctctgtgctc tgcaagccgc
     2401 aaactttcac caatggacca gaactacctg tgaaattaat aacagacata ctccaagctg
     2461 cctttgtgtg cttaatcacg tatactcacg tgctcaatag tcaccaatgc cctccctctt
     2521 ggccctctcc ttttcttttt tcgaccgaat ttcttgaaga cgaaagggcc tcgtgatacg
     2581 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     2641 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     2701 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     2761 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     2821 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     2881 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     2941 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     3001 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     3061 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     3121 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     3181 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     3241 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     3301 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     3361 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     3421 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     3481 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     3541 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     3601 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     3661 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     3721 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3781 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3841 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3901 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     3961 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4021 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4081 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4141 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4201 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4261 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4321 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4381 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     4441 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     4501 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     4561 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     4621 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     4681 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     4741 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga attcgagctc
     4801 ggtacccggg gatcccccgg gctgcaggaa ttcgatatcg tccattccga cagcatcgcc
     4861 agtcactatg gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt
     4921 ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga
     4981 gccactatcg actacgcgat catggcgacc acacccgtcc tgtggatcga taattctcca
     5041 taatagttct gttatttata atctccagca ctaataaatg ctatacgtat atttgtacac
     5101 aatataattt cagaatttat attgctacca tgactgtctg agaattgggg gaataacttg
     5161 ataattgttg ggattccatt gttcgtaaac gcaataatat taggtatata gaagatacta
     5221 aatgttctct ccgaggatat aggaatgctc acaatggaat cgatatattt ctacataata
     5281 gtattgagat tattcctctt ttagttttat ataattcatt atcctattac attatcaatc
     5341 cttgcatttc agcttccatt agacttaatg actgtttctc aatttttatg tcatcttcct
     5401 ggacctcatg tgatactata ccagtagcat gaatactact gaatcgatga tactttagag
     5461 tttcattgca acagtttcaa cacagcctgg catgaacagt ggtaaaagta tttcgtccac
     5521 tattttcggc tactataaat aaatgttttt ttcgcatgca tgt
//
