LOCUS       sequence-272509-        9289 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Packaging plasmid for producing MuLV retroviral particles. Use in 
            conjunction with an envelope plasmid such as pCMV-VSV-G..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9289)
  AUTHORS   Stewart SA, Dykxhoorn DM, Palliser D, Mizuno H, Yu EY, An DS, 
            Sabatini DM, Chen IS, Hahn WC, Sharp PA, Weinberg RA, Novina CD
  TITLE     Lentivirus-delivered stable gene silencing by RNAi in primary cells.
  JOURNAL   RNA 2003 Apr;9(4):493-501.
  PUBMED    12649500
REFERENCE   2  (bases 1 to 9289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA"; date:
            "2003-04"; volume: "9(4)"; pages: "493-501"
COMMENT     Sequence Label: pUMVC
FEATURES             Location/Qualifiers
     source          1..9289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(117..705)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(197..216)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1247..2062)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1247..2062)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1970..1989
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2054..2073
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     2340..2358
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2396..2418)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2518..2537
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2688..3067
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3068..3271
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3222..3242
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3268..3292
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          3407..3676
                     /label=CMV intron
                     /note="modified intron A from human cytomegalovirus"
     regulatory      3721..3730
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3727..5340,5341..5343,5344..8943)
                     /codon_start=1
                     /gene="gag-pol"
                     /product="Moloney murine leukemia virus Gag-Pol 
                     polyprotein"
                     /label=gag-pol
                     /note="contains a read-through stop codon"
                     /translation="MGQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPT
                     FNVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPK
                     PPPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGAKPKPQVLSDSGGPLIDLLTEDPP
                     PYRDPRPPPSDRDGNGGEATPAGEAPDPSPMASRLRGRREPPVADSTTSQAFPLRAGGN
                     GQLQYWPFSSSDLYNWKNNNPSFSEDPGKLTALIESVLITHQPTWDDCQQLLGTLLTGE
                     EKQRVLLEARKAVRGDDGRPTQLPNEVDAAFPLERPDWDYTTQAGRNHLVHYRQLLLAG
                     LQNAGRSPTNLAKVKGITQGPNESPSAFLERLKEAYRRYTPYDPEDPGQETNVSMSFIW
                     QSAPDIGRKLERLEDLKNKTLGDLVREAEKIFNKRETPEEREERIRRETEEKEERRRTE
                     DEQKEKERDRRRHREMSKLLATVVSGQKQDRQGGERRRSQLDRDQCAYCKEKGHWAKDC
                     PKKPRGPRGPRPQTSLLTLDD*GGQGQEPPPEPRITLKVGGQPVTFLVDTGAQHSVLTQ
                     NPGPLSDKSAWVQGATGGKRYRWTTDRKVHLATGKVTHSFLHVPDCPYPLLGRDLLTKL
                     KAQIHFEGSGAQVMGPMGQPLQVLTLNIEDEYRLHETSKEPDVSLGSTWLSDFPQAWAE
                     TGGMGLAVRQAPLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPW
                     NTPLLPVKKPGTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVLDLKD
                     AFFCLRLHPTSQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFDEALHRDLADFRI
                     QHPDLILLQYVDDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGY
                     LLKEGQRWLTEARKETVMGQPTPKTPRQLREFLGTAGFCRLWIPGFAEMAAPLYPLTKT
                     GTLFNWGPDQQKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWR
                     RPVAYLSKKLDPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPP
                     DRWLSNARMTHYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLDILAEAHGTRP
                     DLTDQPLPDADHTWYTDGSSLLQEGQRKAGAAVTTETEVIWAKALPAGTSAQRAELIAL
                     TQALKMAEGKKLNVYTDSRYAFATAHIHGEIYRRRGLLTSEGKEIKNKDEILALLKALF
                     LPKRLSIIHCPGHQKGHSAEARGNRMADQAARKAAITETPDTSTLLIENSSPYTSEHFH
                     YTVTDIKDLTKLGAIYDKTKKYWVYQGKPVMPDQFTFELLDFLHQLTHLSFSKMKALLE
                     RSHSPYYMLNRDRTLKNITETCKACAQVNASKSAVKQGTRVRGHRPGTHWEIDFTEIKP
                     GLYGYKYLLVFIDTFSGWIEAFPTKKETAKVVTKKLLEEIFPRFGMPQVLGTDNGPAFV
                     SKVSQTVADLLGIDWKLHCAYRPQSSGQVERMNRTIKETLTKLTLATGSRDWVLLLPLA
                     LYRARNTPGPHGLTPYEILYGAPPPLVNFPDPDMTRVTNSPSLQAHLQALYLVQHEVWR
                     PLAAAYQEQLDRPVVPHPYRVGDTVWVRRHQTKNLEPRWKGPYPVLLTTPTALKVDGIA
                     AWIHAAHVKAADPGGGPSSRLTWRVQRSQNPLKIRLTREAP"
     primer_bind     4076..4098
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     4114..4130
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    8506..8880
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     8818..8834
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     primer_bind     complement(9022..9041)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9087..9142
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9141..9160)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9235..9252)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
       61 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      121 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      181 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      241 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      301 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      361 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      421 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      481 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      541 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      601 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      661 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      721 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      781 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      841 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
      901 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactcgg gggggggggg
      961 cgctgaggtc tgcctcgtga agaaggtgtt gctgactcat accaggcctg aatcgcccca
     1021 tcatccagcc agaaagtgag ggagccacgg ttgatgagag ctttgttgta ggtggaccag
     1081 ttggtgattt tgaacttttg ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg
     1141 atctgatcct tcaactcagc aaaagttcga tttattcaac aaagccgccg tcccgtcaag
     1201 tcagcgtaat gctctgccag tgttacaacc aattaaccaa ttctgattag aaaaactcat
     1261 cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa
     1321 aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat
     1381 cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct
     1441 cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga
     1501 atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt
     1561 catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac
     1621 gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca
     1681 ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct
     1741 ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga
     1801 taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct
     1861 catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat
     1921 cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc
     1981 atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctc gagcaagacg
     2041 tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt
     2101 ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca
     2161 caacgtggct ttcccccccc ccccattatt gaagcattta tcagggttat tgtctcatga
     2221 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     2281 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     2341 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     2401 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     2461 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     2521 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     2581 taaggagaaa ataccgcatc agattggcta ttggccattg catacgttgt atccatatca
     2641 taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac attgattatt
     2701 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     2761 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     2821 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     2881 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     2941 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     3001 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     3061 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     3121 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     3181 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     3241 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
     3301 acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg
     3361 ccgggaacgg tgcattggaa cgcggattcc ccgtgccaag agtgacgtaa gtaccgccta
     3421 tagactctat aggcacaccc ctttggctct tatgcatgct atactgtttt tggcttgggg
     3481 cctatacacc cccgcttcct tatgctatag gtgatggtat agcttagcct ataggtgtgg
     3541 gttattgacc attattgacc actccaacgg tggagggcag tgtagtctga gcagtactcg
     3601 ttgctgccgc gcgcgccacc agacataata gctgacagac taacagactg ttcctttcca
     3661 tgggtctttt ctgcagtcac cgtcgtcgac ggtatcgata agcttgatat cgaattcgcc
     3721 gccaccatgg gccagactgt taccactccc ttaagtttga ccttaggtca ctggaaagat
     3781 gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga agagacgttg ggttaccttc
     3841 tgctctgcag aatggccaac ctttaacgtc ggatggccgc gagacggcac ctttaaccga
     3901 gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg acacccagac
     3961 caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc ctgggtcaag
     4021 ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc tctccccctt
     4081 gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac tccttctcta
     4141 ggcgccaaac ctaaacctca agttctttct gacagtgggg ggccgctcat cgacctactt
     4201 acagaagacc ccccgcctta tagggaccca agaccacccc cttccgacag ggacggaaat
     4261 ggtggagaag cgacccctgc gggagaggca ccggacccct ccccaatggc atctcgccta
     4321 cgtgggagac gggagccccc tgtggccgac tccactacct cgcaggcatt ccccctccgc
     4381 gcaggaggaa acggacagct tcaatactgg ccgttctcct cttctgacct ttacaactgg
     4441 aaaaataata acccttcttt ttctgaagat ccaggtaaac tgacagctct gatcgagtct
     4501 gtcctcatca cccatcagcc cacctgggac gactgtcagc agctgttggg gactctgctg
     4561 accggagaag aaaaacaacg ggtgctctta gaggctagaa aggcggtgcg gggcgatgat
     4621 gggcgcccca ctcaactgcc caatgaagtc gatgccgctt ttcccctcga gcgcccagac
     4681 tgggattaca ccacccaggc aggtaggaac cacctagtcc actatcgcca gttgctccta
     4741 gcgggtctcc aaaacgcggg cagaagcccc accaatttgg ccaaggtaaa aggaataaca
     4801 caagggccca atgagtctcc ctcggccttc ctagagagac ttaaggaagc ctatcgcagg
     4861 tacactcctt atgaccctga ggacccaggg caagaaacta atgtgtctat gtctttcatt
     4921 tggcagtctg ccccagacat tgggagaaag ttagagaggt tagaagattt aaaaaacaag
     4981 acgcttggag atttggttag agaggcagaa aagatcttta ataaacgaga aaccccggaa
     5041 gaaagagagg aacgtatcag gagagaaaca gaggaaaaag aagaacgccg taggacagag
     5101 gatgagcaga aagagaaaga aagagatcgt aggagacata gagagatgag caagctattg
     5161 gccactgtcg ttagtggaca gaaacaggat agacagggag gagaacgaag gaggtcccaa
     5221 ctcgatcgcg accagtgtgc ctactgcaaa gaaaaggggc actgggctaa agattgtccc
     5281 aagaaaccac gaggacctcg gggaccaaga ccccagacct ccctcctgac cctagatgac
     5341 tagggaggtc agggtcagga gcccccccct gaacccagga taaccctcaa agtcgggggg
     5401 caacccgtca ccttcctggt agatactggg gcccaacact ccgtgctgac ccaaaatcct
     5461 ggacccctaa gtgataagtc tgcctgggtc caaggggcta ctggaggaaa gcggtatcgc
     5521 tggaccacgg atcgcaaagt acatctagct accggtaagg tcacccactc tttcctccat
     5581 gtaccagact gtccctatcc tctgttagga agagatttgc tgactaaact aaaagcccaa
     5641 atccactttg agggatcagg agctcaggtt atgggaccaa tggggcagcc cctgcaagtg
     5701 ttgaccctaa atatagaaga tgagtatcgg ctacatgaga cctcaaaaga gccagatgtt
     5761 tctctagggt ccacatggct gtctgatttt cctcaggcct gggcggaaac cgggggcatg
     5821 ggactggcag ttcgccaagc tcctctgatc atacctctga aagcaacctc tacccccgtg
     5881 tccataaaac aataccccat gtcacaagaa gccagactgg ggatcaagcc ccacatacag
     5941 agactgttgg accagggaat actggtaccc tgccagtccc cctggaacac gcccctgcta
     6001 cccgttaaga aaccagggac taatgattat aggcctgtcc aggatctgag agaagtcaac
     6061 aagcgggtgg aagacatcca ccccaccgtg cccaaccctt acaacctctt gagcgggctc
     6121 ccaccgtccc accagtggta cactgtgctt gatttaaagg atgccttttt ctgcctgaga
     6181 ctccacccca ccagtcagcc tctcttcgcc tttgagtgga gagatccaga gatgggaatc
     6241 tcaggacaat tgacctggac cagactccca cagggtttca aaaacagtcc caccctgttt
     6301 gatgaggcac tgcacagaga cctagcagac ttccggatcc agcacccaga cttgatcctg
     6361 ctacagtacg tggatgactt actgctggcc gccacttctg agctagactg ccaacaaggt
     6421 actcgggccc tgttacaaac cctagggaac ctcgggtatc gggcctcggc caagaaagcc
     6481 caaatttgcc agaaacaggt caagtatctg gggtatcttc taaaagaggg tcagagatgg
     6541 ctgactgagg ccagaaaaga gactgtgatg gggcagccta ctccgaagac ccctcgacaa
     6601 ctaagggagt tcctagggac ggcaggcttc tgtcgcctct ggatccctgg gtttgcagaa
     6661 atggcagccc ccttgtaccc tctcaccaaa acggggactc tgtttaattg gggcccagac
     6721 caacaaaagg cctatcaaga aatcaagcaa gctcttctaa ctgccccagc cctggggttg
     6781 ccagatttga ctaagccctt tgaactcttt gtcgacgaga agcagggcta cgccaaaggt
     6841 gtcctaacgc aaaaactggg accttggcgt cggccggtgg cctacctgtc caaaaagcta
     6901 gacccagtag cagctgggtg gcccccttgc ctacggatgg tagcagccat tgccgtactg
     6961 acaaaggatg caggcaagct aaccatggga cagccactag tcattctggc cccccatgca
     7021 gtagaggcac tagtcaaaca accccccgac cgctggcttt ccaacgcccg gatgactcac
     7081 tatcaggcct tgcttttgga cacggaccgg gtccagttcg gaccggtggt agccctgaac
     7141 ccggctacgc tgctcccact gcctgaggaa gggctgcaac acaactgcct tgatatcctg
     7201 gccgaagccc acggaacccg acccgaccta acggaccagc cgctcccaga cgccgaccac
     7261 acctggtaca cggatggaag cagtctctta caagagggac agcgtaaggc gggagctgcg
     7321 gtgaccaccg agaccgaggt aatctgggct aaagccctgc cagccgggac atccgctcag
     7381 cgggctgaac tgatagcact cacccaggcc ctaaagatgg cagaaggtaa gaagctaaat
     7441 gtttatactg atagccgtta tgcttttgct actgcccata tccatggaga aatatacaga
     7501 aggcgtgggt tgctcacatc agaaggcaaa gagatcaaaa ataaagacga gatcttggcc
     7561 ctactaaaag ccctctttct gcccaaaaga cttagcataa tccattgtcc aggacatcaa
     7621 aagggacaca gcgccgaggc tagaggcaac cggatggctg accaagcggc ccgaaaggca
     7681 gccatcacag agactccaga cacctctacc ctcctcatag aaaattcatc accctacacc
     7741 tcagaacatt ttcattacac agtgactgat ataaaggacc taaccaagtt gggggccatt
     7801 tatgataaaa caaagaagta ttgggtctac caaggaaaac ctgtgatgcc tgaccagttt
     7861 acttttgaat tattagactt tcttcatcag ctgactcacc tcagcttctc aaaaatgaag
     7921 gctctcctag agagaagcca cagtccctac tacatgctga accgggatcg aacactcaaa
     7981 aatatcactg agacctgcaa agcttgtgca caagtcaacg ccagcaagtc tgccgttaaa
     8041 cagggaacta gggtccgcgg gcatcggccc ggcactcatt gggagatcga tttcaccgag
     8101 ataaagcccg gattgtatgg ctataaatat cttctagttt ttatagatac cttttctggc
     8161 tggatagaag ccttcccaac caagaaagaa accgccaagg tcgtaaccaa gaagctacta
     8221 gaggagatct tccccaggtt cggcatgcct caggtattgg gaactgacaa tgggcctgcc
     8281 ttcgtctcca aggtgagtca gacagtggcc gatctgttgg ggattgattg gaaattacat
     8341 tgtgcataca gaccccaaag ctcaggccag gtagaaagaa tgaatagaac catcaaggag
     8401 actttaacta aattaacgct tgcaactggc tctagagact gggtgctcct actcccctta
     8461 gccctgtacc gagcccgcaa cacgccgggc ccccatggcc tcaccccata tgagatctta
     8521 tatggggcac ccccgcccct tgtaaacttc cctgaccctg acatgacaag agttactaac
     8581 agcccctctc tccaagctca cttacaggct ctctacttag tccagcacga agtctggaga
     8641 cctctggcgg cagcctacca agaacaactg gaccgaccgg tggtacctca cccttaccga
     8701 gtcggcgaca cagtgtgggt ccgccgacac cagactaaga acctagaacc tcgctggaaa
     8761 ggaccttacc cagtcctgct gaccaccccc accgccctca aagtagacgg catcgcagct
     8821 tggatacacg ccgcccacgt gaaggctgcc gaccccgggg gtggaccatc ctctagactg
     8881 acatggcgcg ttcaacgctc tcaaaacccc ttaaaaataa ggttaacccg cgaggccccc
     8941 taatcccctt aattcttctg atgctcagag gggtcagtgt gggcccggta ccgtatactc
     9001 tagagcggcc gcggatccag atctttttcc ctctgccaaa aattatgggg acatcatgaa
     9061 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
     9121 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca
     9181 tcagaatgag tatttggttt agagtttggc aacatatgcc cattcttccg cttcctcgct
     9241 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagct
//
