LOCUS       sequence-272527-        6271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-terminal myc-tagged Gamma2 subunit of GABAAR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Arancibia-Carcamo IL, Yuen EY, Muir J, Lumb MJ, Michels G, Saliba 
            RS, Smart TG, Yan Z, Kittler JT, Moss SJ
  TITLE     Ubiquitin-dependent lysosomal targeting of GABA(A) receptors 
            regulates neuronal inhibition.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Oct 13;106(41):17552-7. doi: 
            10.1073/pnas.0905502106. Epub 2009 Oct 6.
  PUBMED    19815531
REFERENCE   2  (bases 1 to 6271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0905502106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-10-13- 13"; volume: "106"; issue: "41"; pages: "17552-7"
COMMENT     Sequence Label: myc-GABAAR Gamma2
FEATURES             Location/Qualifiers
     source          1..6271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        435..453
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     435..452
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     466..485
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             668..757
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             698..787
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             728..817
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             758..847
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     regulatory      2178..2187
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2191..2325
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2228..2247)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2282..2301
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        2394..2751
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2394..2585
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2417..2437)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2602..2737
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2771..2788)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2771..2787)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2780..2802)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3000..3455
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3087..3106)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3297..3318
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3472..3491)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3591..3613
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3651..3669)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3737..3841
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3842..3910,3911..4702)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4060..4079)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4873..5461
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5362..5381
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5615..5632
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5749..5770
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5785..5790,5791..5808,5809..5815)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5823..5839
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5828..5850
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5847..5863
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5847..5863
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        5892..6271
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccgggaa
      301 cggtgcattg gaacgcggat tccccgtgcc aagagtgacg taagtaccgc ctatagagtc
      361 tataggccca cccccttggc ttcgttagaa cgcggctaca attaatacat aaccttatgt
      421 atcatacaca tacgatttag gtgacactat agaataacat ccactttgcc tttctctcca
      481 caggtgtcca ctcccaggtc caactgcacc tcggttctat cgattgaatt ccgccatgag
      541 ttcgccaaat acatggagca ttggaagctc agtctactct cctgtatttt cacagaaaat
      601 gacgctgtgg attctgctcc tgctatcgct ctacccaggc ttcacaagcc aaaagtcaga
      661 tctcgaggaa caaaaactaa tatcagaaga agacctagaa caaaaactaa tatcagaaga
      721 agacctagaa caaaaactaa tatcagaaga agacctagaa caaaaactaa tatcagaaga
      781 agacctagaa caaaaactaa tatcagaaga agacctagaa caaaaactaa tatcagaaga
      841 agacctagat gactatgaag attacacttc taataaaaca tgggtgttga ctccaaaagt
      901 tccagagggt gatgtcactg tcatcttaaa caacctgctg gaaggctatg acaacaaact
      961 tcgacctgac atcggagtga aaccaacatt aattcataca gatatgtatg tgaacagcat
     1021 tggtccagtg aatgctatca atatggaata tacaattgat attttttttg cccaaacctg
     1081 gtatgacaga cgtttgaaat ttaacagtac cattaaagtt ctccggttga atagcaatat
     1141 ggtggggaaa atctggattc cagacacttt cttcaggaac tccaaaaagg ctgatgctca
     1201 ctggatcacc actcccaaca ggatgctgag aatttggaat gatggtcgag ttctctacac
     1261 cttaaggcta acaattgatg ctgagtgcca gttgcaatta cacaacttcc caatggatga
     1321 acactcctgc cccctggagt tctccagtta tggatatcct cgtgaagaaa ttgtttatca
     1381 atggaagcgc agttctgttg aagtgggtga cacaagatca tggaggctgt atcaattttc
     1441 ctttgttgga ttgaggaata caactgaagt agtgaagaca acttctggtg actatgtggt
     1501 gatgtctgtg tacttcgatc tgagcagaag aatgggctac ttcaccatcc agacttacat
     1561 tccctgcaca ctcatcgtgg tcctgtcctg ggtgtccttc tggatcaata aggatgctgt
     1621 tcctgccaga acatctttag gaatcactac tgtcctgacc atgacaactt taagcaccat
     1681 agccagaaaa tctctgccca aggtctccta tgtcacagca atggatctct ttgtatctgt
     1741 ttgcttcatc tttgtgtttt ctgctttggt ggagtatggc accctgcatt attttgtcag
     1801 caaccggaag ccaagcaagg ataaagacaa aaagaagaaa aaccctcttc ttcggatgtt
     1861 ttccttcaag gcccctacca ttgatattcg tcccagatca gcaaccattc aaatgaacaa
     1921 tgccacacac cttcaagaga gggatgaaga atatggctat gagtgtttgg atggcaagga
     1981 ctgtgccagt ttcttctgct gttttgaaga ttgccgaaca ggagcctgga gacatgggag
     2041 gatacatatt cgcattgcca aaatggactc ctatgctcgg atcttcttcc ctaccgcctt
     2101 ttgcttgttc aatcttgttt actgggtctc ctatctttat ctgtaaggat cctctagagt
     2161 cgacctgcag aagcttggcc gccatggccc aacttgttta ttgcagctta taatggttac
     2221 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     2281 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcgggaa ttaattcggc
     2341 gcagcaccat ggcctgaaat aacctctgaa agaggaactt ggttaggtac cttctgaggc
     2401 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     2461 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     2521 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     2581 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     2641 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     2701 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctgtta
     2761 acagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     2821 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     2881 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
     2941 attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta
     3001 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     3061 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     3121 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     3181 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
     3241 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     3301 actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata
     3361 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     3421 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
     3481 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     3541 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     3601 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     3661 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     3721 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     3781 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     3841 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     3901 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     3961 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4021 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     4081 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4141 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     4201 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     4261 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     4321 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     4381 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     4441 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     4501 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     4561 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     4621 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     4681 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     4741 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     4801 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     4861 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4921 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4981 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5041 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5101 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5161 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5221 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     5281 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5341 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     5401 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     5461 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     5521 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     5581 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     5641 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     5701 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     5761 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     5821 aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta cgaattaatt
     5881 cgagctcgcc cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     5941 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     6001 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     6061 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     6121 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     6181 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     6241 catctacgta ttagtcatcg ctattaccat g
//
