LOCUS       sequence-272531-       12109 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PspCas13b in mammalian cells, localized in the nucleus. 
            For cloning of guide RNAs compatible with PspCas13b. Contains a 3' 
            direct repeat. Clone using BsmBI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12109)
  AUTHORS   Wessels HH, Mendez-Mancilla A, Guo X, Legut M, Daniloski Z, Sanjana 
            NE
  TITLE     Massively parallel Cas13 screens reveal principles for guide RNA 
            design.
  JOURNAL   Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 
            10.1038/s41587-020-0456-9. Epub 2020 Mar 16.
  PUBMED    32518401
REFERENCE   2  (bases 1 to 12109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0456-9"; journalName: "Nat Biotechnol"; date: 
            "2020-06"; volume: "38"; issue: "6"; pages: "722-727"
COMMENT     Sequence Label: pLentiRNACRISPR_003 -
            hU6-DR_BsmBI-EFS-PspCas1...
FEATURES             Location/Qualifiers
     source          1..12109
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2371..2619
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2371..2391
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2542..2561
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2732..2943
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             6238..6285
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6295..6351
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6352..6948
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6845..6865
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6964..7552
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7017..7037)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7435..7446)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7624..7857
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7883..7900)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7889..8113
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8159..8587
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8246..8265)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8456..8477
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8596..8616)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8601..8930
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8781..8916
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8843..8862
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8978..9025
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9044..9418
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9548..9669
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9585..9604)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9639..9658
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9718..9734)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9718..9734)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9731..9753)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9742..9758
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9766..9772,9773..9790,9791..9796))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9811..9832
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9949..9966)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10120..10708)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10200..10219)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10879..11670,11671..11739))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11502..11521
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11740..11844)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11919..11938)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaaggtacc gagggcctat ttcccatgat tccttcatat
     2401 ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac tgtaaacaca
     2461 aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt
     2521 ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat
     2581 ttcttggctt tatatatctt gtggaaagga cgaaacaccg agacggacgt ctctgttgtg
     2641 gaaggtccag ttttgagggg ctattacaac ttttttgaat tcgctagcta ggtcttgaaa
     2701 ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     2761 gagaagttgg ggggaggggt cggcaattga tccggtgcct agagaaggtg gcgcggggta
     2821 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     2881 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     2941 caggaccggt tctagagcgc tgccaccatg aacatccccg ctctggtgga aaaccagaag
     3001 aagtactttg gcacctacag cgtgatggcc atgctgaacg ctcagaccgt gctggaccac
     3061 atccagaagg tggccgatat tgagggcgag cagaacgaga acaacgagaa tctgtggttt
     3121 caccccgtga tgagccacct gtacaacgcc aagaacggct acgacaagca gcccgagaaa
     3181 accatgttca tcatcgagcg gctgcagagc tacttcccat tcctgaagat catggccgag
     3241 aaccagagag agtacagcaa cggcaagtac aagcagaacc gcgtggaagt gaacagcaac
     3301 gacatcttcg aggtgctgaa gcgcgccttc ggcgtgctga agatgtacag ggacctgacc
     3361 aaccactaca agacctacga ggaaaagctg aacgacggct gcgagttcct gaccagcaca
     3421 gagcaacctc tgagcggcat gatcaacaac tactacacag tggccctgcg gaacatgaac
     3481 gagagatacg gctacaagac agaggacctg gccttcatcc aggacaagcg gttcaagttc
     3541 gtgaaggacg cctacggcaa gaaaaagtcc caagtgaata ccggattctt cctgagcctg
     3601 caggactaca acggcgacac acagaagaag ctgcacctga gcggagtggg aatcgccctg
     3661 ctgatctgcc tgttcctgga caagcagtac atcaacatct ttctgagcag gctgcccatc
     3721 ttctccagct acaatgccca gagcgaggaa cggcggatca tcatcagatc cttcggcatc
     3781 aacagcatca agctgcccaa ggaccggatc cacagcgaga agtccaacaa gagcgtggcc
     3841 atggatatgc tcaacgaagt gaagcggtgc cccgacgagc tgttcacaac actgtctgcc
     3901 gagaagcagt cccggttcag aatcatcagc gacgaccaca atgaagtgct gatgaagcgg
     3961 agcagcgaca gattcgtgcc tctgctgctg cagtatatcg attacggcaa gctgttcgac
     4021 cacatcaggt tccacgtgaa catgggcaag ctgagatacc tgctgaaggc cgacaagacc
     4081 tgcatcgacg gccagaccag agtcagagtg atcgagcagc ccctgaacgg cttcggcaga
     4141 ctggaagagg ccgagacaat gcggaagcaa gagaacggca ccttcggcaa cagcggcatc
     4201 cggatcagag acttcgagaa catgaagcgg gacgacgcca atcctgccaa ctatccctac
     4261 atcgtggaca cctacacaca ctacatcctg gaaaacaaca aggtcgagat gtttatcaac
     4321 gacaaagagg acagcgcccc actgctgccc gtgatcgagg atgatagata cgtggtcaag
     4381 acaatcccca gctgccggat gagcaccctg gaaattccag ccatggcctt ccacatgttt
     4441 ctgttcggca gcaagaaaac cgagaagctg atcgtggacg tgcacaaccg gtacaagaga
     4501 ctgttccagg ccatgcagaa agaagaagtg accgccgaga atatcgccag cttcggaatc
     4561 gccgagagcg acctgcctca gaagatcctg gatctgatca gcggcaatgc ccacggcaag
     4621 gatgtggacg ccttcatcag actgaccgtg gacgacatgc tgaccgacac cgagcggaga
     4681 atcaagagat tcaaggacga ccggaagtcc attcggagcg ccgacaacaa gatgggaaag
     4741 agaggcttca agcagatctc cacaggcaag ctggccgact tcctggccaa ggacatcgtg
     4801 ctgtttcagc ccagcgtgaa cgatggcgag aacaagatca ccggcctgaa ctaccggatc
     4861 atgcagagcg ccattgccgt gtacgatagc ggcgacgatt acgaggccaa gcagcagttc
     4921 aagctgatgt tcgagaaggc ccggctgatc ggcaagggca caacagagcc tcatccattt
     4981 ctgtacaagg tgttcgcccg cagcatcccc gccaatgccg tcgagttcta cgagcgctac
     5041 ctgatcgagc ggaagttcta cctgaccggc ctgtccaacg agatcaagaa aggcaacaga
     5101 gtggatgtgc ccttcatccg gcgggaccag aacaagtgga aaacacccgc catgaagacc
     5161 ctgggcagaa tctacagcga ggatctgccc gtggaactgc ccagacagat gttcgacaat
     5221 gagatcaagt cccacctgaa gtccctgcca cagatggaag gcatcgactt caacaatgcc
     5281 aacgtgacct atctgatcgc cgagtacatg aagagagtgc tggacgacga cttccagacc
     5341 ttctaccagt ggaaccgcaa ctaccggtac atggacatgc ttaagggcga gtacgacaga
     5401 aagggctccc tgcagcactg cttcaccagc gtggaagaga gagaaggcct ctggaaagag
     5461 cgggcctcca gaacagagcg gtacagaaag caggccagca acaagatccg cagcaaccgg
     5521 cagatgagaa acgccagcag cgaagagatc gagacaatcc tggataagcg gctgagcaac
     5581 agccggaacg agtaccagaa aagcgagaaa gtgatccggc gctacagagt gcaggatgcc
     5641 ctgctgtttc tgctggccaa aaagaccctg accgaactgg ccgatttcga cggcgagagg
     5701 ttcaaactga aagaaatcat gcccgacgcc gagaagggaa tcctgagcga gatcatgccc
     5761 atgagcttca ccttcgagaa aggcggcaag aagtacacca tcaccagcga gggcatgaag
     5821 ctgaagaact acggcgactt ctttgtgctg gctagcgaca agaggatcgg caacctgctg
     5881 gaactcgtgg gcagcgacat cgtgtccaaa gaggatatca tggaagagtt caacaaatac
     5941 gaccagtgca ggcccgagat cagctccatc gtgttcaacc tggaaaagtg ggccttcgac
     6001 acataccccg agctgtctgc cagagtggac cgggaagaga aggtggactt caagagcatc
     6061 ctgaaaatcc tgctgaacaa caagaacatc aacaaagagc agagcgacat cctgcggaag
     6121 atccggaacg ccttcgatca caacaattac cccgacaaag gcgtggtgga aatcaaggcc
     6181 ctgcctgaga tcgccatgag catcaagaag gcctttgggg agtacgccat catgaagaag
     6241 cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaaggatc cggcgcaaca
     6301 aacttctctc tgctgaaaca agccggagat gtcgaagaga atcctggacc gaccgagtac
     6361 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     6421 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     6481 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     6541 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     6601 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     6661 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     6721 ctggccaccg tcggagtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     6781 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     6841 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     6901 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaac gcgttaagtc
     6961 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     7021 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     7081 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     7141 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     7201 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     7261 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     7321 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     7381 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     7441 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     7501 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt
     7561 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     7621 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     7681 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     7741 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     7801 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
     7861 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     7921 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     7981 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     8041 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     8101 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     8161 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     8221 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     8281 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     8341 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     8401 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     8461 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     8521 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     8581 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     8641 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     8701 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     8761 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     8821 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     8881 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     8941 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     9001 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
     9061 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     9121 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     9181 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     9241 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     9301 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     9361 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
     9421 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     9481 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     9541 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     9601 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     9661 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     9721 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     9781 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     9841 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     9901 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     9961 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    10021 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    10081 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    10141 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    10201 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    10261 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    10321 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    10381 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    10441 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    10501 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    10561 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    10621 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    10681 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    10741 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    10801 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    10861 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    10921 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    10981 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    11041 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    11101 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    11161 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    11221 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    11281 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    11341 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    11401 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    11461 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    11521 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    11581 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    11641 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    11701 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    11761 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    11821 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
    11881 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
    11941 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
    12001 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
    12061 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgtt
//
