LOCUS       sequence-27254-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral RNAi empty control vector, 3'LTR insertion, Puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti X2 Puro Empty (w609-1)
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      62..197
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    237..358
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(248..267)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     302..321
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             430..610
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     protein_bind    685..709
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(774..798)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gttgtttttt ggcactagcg cgcgtatcga ctcactatag ggcgaattgg gtacgtccct
       61 aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg
      121 gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc
      181 ggagttaggg gcgggatggg cggagttagg ggcgggatag ctagagccag acatgataag
      241 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
      301 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttcc
      361 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg
      421 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac
      481 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag
      541 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc
      601 agagagaccc agtacaagca aaaagcagat cttgtcttcg ttgggagtga gctagtccag
      661 tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagcaggctt taaaggaacc
      721 aattcagtcg actggatcgg taccgaattc gcggccgcac tcgagatatc tagacccagc
      781 tttcttgtac aaagtggttg atatccagca cagtggcggc cgctcgagtc tagccctttc
      841 agtcccccct tttcttttaa aagtggctaa gatctacaag ctgccttgtt agttcattga
      901 gtcattaaag agtataccga ggacc
//
