LOCUS       sequence-27255-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3'LTR 
            insertion, Hygro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti X2 Hygro/pTER shLUC (w607-1)
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    33..51
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(184..208)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agtgatgtct ttggatttgg gatcttataa gttccctatc agtgatagag atccccttac
       61 gctgagtact tcgagtgtgc tgtcctcgaa gtactcagcg taagtttttg gaaaagcttg
      121 gtaccgagct cggatccact agtccagtgt ggtggaattc gcggccgcac tcgagatatc
      181 tagacccagc tttcttgtac aaagtggttg atatccagca cagtggcggc cgctcgagtc
      241 tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta
      301 agtcattggt cttaaaggta ccgggccctc gacggatctg cacccaatcg gcaggcacgg
      361 gcggcgatct ccaatctgcg ggatcagtca gatcacccga gtgcgtgggc atgacaatcg
      421 tgccctgggg accaacacaa tccagaaggg cctgaatcac tgcgaccggc cctcccgcga
      481 cccagccgag cgagcttagc gaactgtgga cgagaactgt gccaccaagc gtaaggccgt
      541 tctctcgcat ttgccttgct aggctcgcgc gagttgctgg ctgaggcgtt ctcgaaatca
      601 gctcttgttc ggtcggcatc tactctattc ctttgccctc ggacgagtgc tggggcgtcg
      661 gtttccacta tcggcgagta cttccgacac agccatcggt ccagacggcc gcgcttctgc
      721 gggcgatttg tgtacgcccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga
      781 cctgcggccc aagctgcatc atcgaaattg ccgtcaacca agctctgata gagtggtcaa
      841 gacaatgcga gcatatacgc ccgagcgcgg cgatcctgcc aagctccgga atgccctccg
      901 cctcga
//
