LOCUS       sequence-27256-e         640 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3'LTR 
            insertion, Blast .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti X2 Blast/pTER shLUC (w618-1)
FEATURES             Location/Qualifiers
     source          1..640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    33..51
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(184..208)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agtgatgtct ttggatttgg gatcttataa gttccctatc agtgatagag atccccttac
       61 gctgagtact tcgagtgtgc tgtcctcgaa gtactcagcg taagtttttg gaaaagcttg
      121 gtaccgagct cggatccact agtccagtgt ggtggaattc gcggccgcac tcgagatatc
      181 tagacccagc tttcttgtac aaagtggttg atatccagca cagtggcggc cgctcgagtc
      241 tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta
      301 agtcattggt cttaaaggta ccgagctcga attgtgctta gccctcccac acataaccag
      361 agggcagcaa ttcacgaatc ccaactgccg tcggctgtcc atcactgtcc ttcactatgg
      421 ctttgatccc aggatgcaga tcgagaagca cctgtcggca ccgtccgcag gggctcaaga
      481 tgcccctgtt ctcatttccg atcgcgacga tacaagtcag gttgccagct gccgcagcag
      541 cagcagtgcc cagcaccacg agttctgcac aaggtccccc agtaaaatga tatacattga
      601 caccagtgaa gatgcggccg tcgctagaga gagctgcgct
//
