LOCUS       sequence-27258-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3'LTR 
            insertion, Puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti X2 Puro/pTER shLUC (w589-1)
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    46..64
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(197..221)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(485..505)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gaaagggggc ttccgtgatg tctttggatt tgggatctta taagttccct atcagtgata
       61 gagatcccct tacgctgagt acttcgagtg tgctgtcctc gaagtactca gcgtaagttt
      121 ttggaaaagc ttggtaccga gctcggatcc actagtccag tgtggtggaa ttcgcggccg
      181 cactcgagat atctagaccc agctttcttg tacaaagtgg ttgatatcca gcacagtggc
      241 ggccgctcga gtctagccct tccagtcccc ccttttcttt taaaaagtgg ctaagatcta
      301 cagctgcctt gtaagtcatt ggtcttaaag gtaccgggcc ctcgagatgc atggggtcgt
      361 gcgctccttt cggtcgggcg ctgcgggtcg tggggcgggc gtcaggcacc gggcttgcgg
      421 gtcatgcacc aggtgcgcgg tccttcgggc acctcgacgt cggcggtgac ggtgaagccg
      481 agccgctcgt agaaggggag gttgcggggc gcggaggtct ccaggaaggc gggcacaccg
      541 gcgcggacgg ccacctccac accggggaac acgacggcac tacccacacg attgcacagg
      601 tggtcggaca agacgccgaa aaagacataa cataaaaaca acaaccttga gcatgagaag
      661 aaaacgagcc aaacgtaaga tagcaaaggc gacgatgaga tctgtaacaa tgaaccacaa
      721 tcgatggaaa gacacgatac atactaaaga caatgcagca gtgagaaaaa aaaggagatg
      781 aagagtgtag atagacagca actagcagta ttaagagaat acacgcgaga acacgaaaaa
      841 aacgaataga ttattatata ttataaaaat ttactgatat caagaatgat agtaaaagaa
      901 ataat
//
