LOCUS       sequence-27259-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral V5-Luciferase expression vector, CMV promoter, 
            Blast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti CMV V5-LUC Blast (w567-1)
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(116..157)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(169..193)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(277..301)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(298..501)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(327..347)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        502..805
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atggcgccgg gcctttcttt atgtttttgg cgtcttccat ggtggcttta ccaacagtac
       61 cggaatgcca agctttttgc aaaagcctag ggactgaatt ggttcccatg gatcccgtag
      121 aatcgagacc gaggagaggg ttagggatag gcttaccttc catggtggag cctgcttttt
      181 tgtacaaact tgttgatatc tgcagaattc caccacactg gactagagtc ggtgtcttct
      241 atggaggtca aaacagcgtg gatggcgtct ccaggcgatc tgacggttca ctaaacgagc
      301 tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga
      361 gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac
      421 gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat
      481 gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca
      541 agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc
      601 attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc
      661 agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat
      721 gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg
      781 gccatttacc gtaagttatg taacgcgg
//
