LOCUS       sequence-272590-       15873 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15873)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 15873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..15873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        631..740
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     regulatory      9614..9623
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10309..10332
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             10354..10383
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             10384..10407
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(10497..10517)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10915..10926)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    11151..11285
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11188..11207)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11242..11261
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  11314..11479
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      11663..12117
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11750..11769)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11959..11980
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12143..12247
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12248..12316,12317..13108)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12466..12485)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13279..13867
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13768..13787
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14021..14038
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  14172..14396
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(14463..14996)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(15185..15190,15191..15207,15208..15213))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    15721..15769
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     15794..15813
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaatacg gacctttaat tcaacccaac acaatatatt
      661 atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac tatactgtaa
      721 attacatttt atttacaatc actcgacgga tccttaagaa ggagatatac tatggcgacc
      781 ctggaaaagc tgatgaaggc cttcgagtcc ctcaagtcct tccaacagca gcaacagcaa
      841 caacagcagc aacagcaaca acagcagcaa cagcaacaac agcagcaaca gcaacaacag
      901 cagcaacagc aacaacagca gcaacagcaa caacagcagc aacagcaaca acagcagcaa
      961 cagcaacaac agcagcaaca gcaaccgcca ccgccgccgc cgccgccgcc gcctcctcag
     1021 cttcctcagc cgccgccgca ggcacagccg ctgctgcctc agccgcagcc gcccccgccg
     1081 ccgcccccgc cgccacccgg cccggctgtg gctgaggagc cgctgcaccg accaaagaaa
     1141 gaactttcag ctaccaagaa agaccgtgtg aatcattgtc tgacaatatg tgaaaacata
     1201 gtggcacagt ctgtcagaaa ttctccagaa tttcagaaac ttctgggcat cgctatggaa
     1261 ctttttctgc tgtgcagtga tgacgcagag tcagatgtca ggatggtggc tgacgaatgc
     1321 ctcaacaaag ttatcaaagc tttgatggat tctaatcttc caaggttaca gctcgagctc
     1381 tataaggaaa ttaaaaagaa tggtgcccct cggagtttgc gtgctgccct gtggaggttt
     1441 gctgagctgg ctcacctggt tcggcctcag aaatgcaggc cttacctggt gaaccttctg
     1501 ccgtgcctga ctcgaacaag caagagaccc gaagaatcag tccaggagac cttggctgca
     1561 gctgttccca aaattatggc ttcttttggc aattttgcaa atgacaatga aattaaggtt
     1621 ttgttaaagg ccttcatagc gaacctgaag tcaagctccc ccaccattcg gcggacagcg
     1681 gctggatcag cagtgagcat ctgccagcac tcaagaagga cacaatattt ctatagttgg
     1741 ctactaaatg tgctcttagg cttactcgtt cctgtcgagg atgaacactc cactctgctg
     1801 attcttggcg tgctgctcac cctgaggtat ttggtgccct tgctgcagca gcaggtcaag
     1861 gacacaagcc tgaaaggcag cttcggagtg acaaggaaag aaatggaagt ctctccttct
     1921 gcagagcagc ttgtccaggt ttatgaactg acgttacatc atacacagca ccaagaccac
     1981 aatgttgtga ccggagccct ggagctgttg cagcagctct tcagaacgcc tccacccgag
     2041 cttctgcaaa ccctgaccgc agtcgggggc attgggcagc tcaccgctgc taaggaggag
     2101 tctggtggcc gaagccgtag tgggagtatt gtggaactta tagctggagg gggttcctca
     2161 tgcagccctg tcctttcaag aaaacaaaaa ggcaaagtgc tcttaggaga agaagaagcc
     2221 ttggaggatg actctgaatc gagatcggat gtcagcagct ctgccttaac agcctcagtg
     2281 aaggatgaga tcagtggaga gctggctgct tcttcagggg tttccactcc agggtcagca
     2341 ggtcatgaca tcatcacaga acagccacgg tcacagcaca cactgcaggc ggactcagtg
     2401 gatctggcca gctgtgactt gacaagctct gccactgatg gggatgagga ggatatcttg
     2461 agccacagct ccagccaggt cagcgccgtc ccatctgacc ctgccatgga cctgaatgat
     2521 gggacccagg cctcgtcgcc catcagcgac agctcccaga ccaccaccga agggcctgat
     2581 tcagctgtta ccccttcaga cagttctgaa attgtgttag acggtaccga caaccagtat
     2641 ttgggcctgc agattggaca gccccaggat gaagatgagg aagccacagg tattcttcct
     2701 gatgaagcct cggaggcctt caggaactct tccatggccc ttcaacaggc acatttattg
     2761 aaaaacatga gtcactgcag gcagccttct gacagcagtg ttgataaatt tgtgttgaga
     2821 gatgaagcta ctgaaccggg tgatcaagaa aacaagcctt gccgcatcaa aggtgacatt
     2881 ggacagtcca ctgatgatga ctctgcacct cttgtccatt gtgtccgcct tttatctgct
     2941 tcgtttttgc taacaggggg aaaaaatgtg ctggttccgg acagggatgt gagggtcagc
     3001 gtgaaggccc tggccctcag ctgtgtggga gcagctgtgg ccctccaccc ggaatctttc
     3061 ttcagcaaac tctataaagt tcctcttgac accacggaat accctgagga acagtatgtc
     3121 tcagacatct tgaactacat cgatcatgga gacccacagg ttcgaggagc cactgccatt
     3181 ctctgtggga ccctcatctg ctccatcctc agcaggtccc gcttccacgt gggagattgg
     3241 atgggcacca ttagaaccct cacaggaaat acattttctt tggcggattg cattcctttg
     3301 ctgcggaaaa cactgaagga tgagtcttct gttacttgca agttagcttg tacagctgtg
     3361 aggaactgtg tcatgagtct ctgcagcagc agctacagtg agttaggact gcagctgatc
     3421 atcgatgtgc tgactctgag gaacagttcc tattggctgg tgaggacaga gcttctggaa
     3481 acccttgcag agattgactt caggctggtg agctttttgg aggcaaaagc agaaaactta
     3541 cacagagggg ctcatcatta tacagggctt ttaaaactgc aagaacgagt gctcaataat
     3601 gttgtcatcc atttgcttgg agatgaagac cccagggtgc gacatgttgc cgcagcatca
     3661 ctaattaggc ttgtcccaaa gctgttttat aaatgtgacc aaggacaagc tgatccagta
     3721 gtggccgtgg caagagatca aagcagtgtt tacctgaaac ttctcatgca tgagacgcag
     3781 cctccatctc atttctccgt cagcacaata accagaatat atagaggcta taacctacta
     3841 ccaagcataa cagacgtcac tatggaaaat aacctttcaa gagttattgc agcagtttct
     3901 catgaactaa tcacatcaac caccagagca ctcacatttg gatgctgtga agctttgtgt
     3961 cttctttcca ctgccttccc agtttgcatt tggagtttag gttggcactg tggagtgcct
     4021 ccactgagtg cctcagatga gtctaggaag agctgtaccg ttgggatggc cacaatgatt
     4081 ctgaccctgc tctcgtcagc ttggttccca ttggatctct cagcccatca agatgctttg
     4141 attttggccg gaaacttgct tgcagccagt gctcccaaat ctctgagaag ttcatgggcc
     4201 tctgaagaag aagccaaccc agcagccacc aagcaagagg aggtctggcc agccctgggg
     4261 gaccgggccc tggtgcccat ggtggagcag ctcttctctc acctgctgaa ggtgattaac
     4321 atttgtgccc acgtcctgga tgacgtggct cctggacccg caataaaggc agccttgcct
     4381 tctctaacaa accccccttc tctaagtccc atccgacgaa aggggaagga gaaagaacca
     4441 ggagaacaag catctgtacc gttgagtccc aagaaaggca gtgaggccag tgcagcttct
     4501 agacaatctg atacctcagg tcctgttaca acaagtaaat cctcatcact ggggagtttc
     4561 tatcatcttc cttcatacct caaactgcat gatgtcctga aagctacaca cgctaactac
     4621 aaggtcacgc tggatcttca gaacagcacg gaaaagtttg gagggtttct tcgctcagcc
     4681 ttggatgttc tttctcagat actagagctg gccacactgc aggacattgg gaagtgtgtt
     4741 gaagagatcc taggatacct gaaatcctgc tttagtcgag aaccaatgat ggcaactgtt
     4801 tgtgttcaac aattgttgaa gactctcttt ggcacaaact tggcctccca gtttgatggc
     4861 ttatcttcca accccagcaa gtcacaaggc cgagcacagc gccttggctc ctccagtgtg
     4921 aggccaggct tgtaccacta ctgcttcatg gccccgtaca cccacttcac ccaggccctc
     4981 gctgacgcca gcctgaggaa catggtgcag gcggagcagg agaacgacac ctcgggatgg
     5041 tttgatgtcc tccagaaagt gtctacccag ttgaagacaa acctcacgag tgtcacaaag
     5101 aaccgtgcag ataagaatgc tattcataat cacattcgtt tgtttgaacc tcttgttata
     5161 aaagctttaa aacagtacac gactacaaca tgtgtgcagt tacagaagca ggttttagat
     5221 ttgctggcgc agctggttca gttacgggtt aattactgtc ttctggattc agatcaggtg
     5281 tttattggct ttgtattgaa acagtttgaa tacattgaag tgggccagtt cagggaatca
     5341 gaggcaatca ttccaaacat ctttttcttc ttggtattac tatcttatga acgctatcat
     5401 tcaaaacaga tcattggaat tcctaaaatc attcagctct gtgatggcat catggccagt
     5461 ggaaggaagg ctgtgacaca tgccataccg gctctgcagc ccatagtcca cgacctcttt
     5521 gtattaagag gaacaaataa agctgatgca ggaaaagagc ttgaaaccca aaaagaggtg
     5581 gtggtgtcaa tgttactgag actcatccag taccatcagg tgttggagat gttcattctt
     5641 gtcctgcagc agtgccacaa ggagaatgaa gacaagtgga agcgactgtc tcgacagata
     5701 gctgacatca tcctcccaat gttagccaaa cagcagatgc acattgactc tcatgaagcc
     5761 cttggagtgt taaatacatt atttgagatt ttggcccctt cctccctccg tccggtagac
     5821 atgcttttac ggagtatgtt cgtcactcca aacacaatgg cgtccgtgag cactgttcaa
     5881 ctgtggatat cgggaattct ggccattttg agggttctga tttcccagtc aactgaagat
     5941 attgttcttt ctcgtattca ggagctctcc ttctctccgt atttaatctc ctgtacagta
     6001 attaataggt taagagatgg ggacagtact tcaacgctag aagaacacag tgaagggaaa
     6061 caaataaaga atttgccaga agaaacattt tcaaggtttc tattacaact ggttggtatt
     6121 cttttagaag acattgttac aaaacagctg aaggtggaaa tgagtgagca gcaacatact
     6181 ttctattgcc aggaactagg cacactgcta atgtgtctga tccacatctt caagtctgga
     6241 atgttccgga gaatcacagc agctgccact aggctgttcc gcagtgatgg ctgtggcggc
     6301 agtttctaca ccctggacag cttgaacttg cgggctcgtt ccatgatcac cacccacccg
     6361 gccctggtgc tgctctggtg tcagatactg ctgcttgtca accacaccga ctaccgctgg
     6421 tgggcagaag tgcagcagac cccgaaaaga cacagtctgt ccagcacaaa gttacttagt
     6481 ccccagatgt ctggagaaga ggaggattct gacttggcag ccaaacttgg aatgtgcaat
     6541 agagaaatag tacgaagagg ggctctcatt ctcttctgtg attatgtctg tcagaacctc
     6601 catgactccg agcacttaac gtggctcatt gtaaatcaca ttcaagatct gatcagcctt
     6661 tcccacgagc ctccagtaca ggacttcatc agtgccgttc atcggaactc tgctgccagc
     6721 ggcctgttca tccaggcaat tcagtctcgt tgtgaaaacc tttcaactcc aaccatgctg
     6781 aagaaaactc ttcagtgctt ggaggggatc catctcagcc agtcgggagc tgtgctcacg
     6841 ctgtatgtgg acaggcttct gtgcacccct ttccgtgtgc tggctcgcat ggtcgacatc
     6901 cttgcttgtc gccgggtaga aatgcttctg gctgcaaatt tacagagcag catggcccag
     6961 ttgccaatgg aagaactcaa cagaatccag gaataccttc agagcagcgg gctcgctcag
     7021 agacaccaaa ggctctattc cctgctggac aggtttcgtc tctccaccat gcaagactca
     7081 cttagtccct ctcctccagt ctcttcccac ccgctggacg gggatgggca cgtgtcactg
     7141 gaaacagtga gtccggacaa agactggtac gttcatcttg tcaaatccca gtgttggacc
     7201 aggtcagatt ctgcactgct ggaaggtgca gagctggtga atcggattcc tgctgaagat
     7261 atgaatgcct tcatgatgaa ctcggagttc aacctaagcc tgctagctcc atgcttaagc
     7321 ctagggatga gtgaaatttc tggtggccag aagagtgccc tttttgaagc agcccgtgag
     7381 gtgactctgg cccgtgtgag cggcaccgtg cagcagctcc ctgctgtcca tcatgtcttc
     7441 cagcccgagc tgcctgcaga accggcggcc tactggagca agttgaatga tctgtttggg
     7501 gatgctgcac tgtatcagtc cctgcccact ctggcccggg ccctggcaca gtacctggtg
     7561 gtggtctcca aactgcccag tcatttgcac cttcctcctg agaaagagaa ggacattgtg
     7621 aaattcgtgg tggcaaccct tgaggccctg tcctggcatt tgatccatga gcagatcccg
     7681 ctgagtctgg atctccaggc agggctggac tgctgctgcc tggccctgca gctgcctggc
     7741 ctctggagcg tggtctcctc cacagagttt gtgacccacg cctgctccct catccactgt
     7801 gtgcacttca tcctggaggc cgttgcagtg cagcctggag agcagcttct tagtccagaa
     7861 agaaggacaa ataccccaaa agccatcagc gaggaggagg aggaagtaga tccaaacaca
     7921 cagaatccta agtatatcac tgcagcctgt gagatggtgg cagaaatggt ggagtctctg
     7981 cagtcggtgt tggccttggg tcataaaagg aatagcggcg tgccggcgtt tctcacgcca
     8041 ttgctcagga acatcatcat cagcctggcc cgcctgcccc ttgtcaacag ctacacacgt
     8101 gtgcccccac tggtgtggaa gcttggatgg tcacccaaac cgggagggga ttttggcaca
     8161 gcattccctg agatccccgt ggagttcctc caggaaaagg aagtctttaa ggagttcatc
     8221 taccgcatca acacactagg ctggaccagt cgtactcagt ttgaagaaac ttgggccacc
     8281 ctccttggtg tcctggtgac gcagcccctc gtgatggagc aggaggagag cccaccagaa
     8341 gaagacacag agaggaccca gatcaacgtc ctggccgtgc aggccatcac ctcactggtg
     8401 ctcagtgcaa tgactgtgcc tgtggccggc aacccagctg taagctgctt ggagcagcag
     8461 ccccggaaca agcctctgaa agctctcgac accaggtttg ggaggaagct gagcattatc
     8521 agagggattg tggagcaaga gattcaagca atggtttcaa agagagagaa tattgccacc
     8581 catcatttat atcaggcatg ggatcctgtc ccttctctgt ctccggctac tacaggtgcc
     8641 ctcatcagcc acgagaagct gctgctgcag atcaaccccg agcgggagct ggggagcatg
     8701 agctacaaac tcggccaggt gtccatacac tccgtgtggc tagggaacag catcacaccc
     8761 ctgagggagg aggaatggga cgaggaagag gaggaggagg ccgacgcccc tgcaccttcg
     8821 tcaccaccca cgtctccagt caactccagg aaacaccggg ctggagttga catccactcc
     8881 tgttcgcagt ttttgcttga gttgtacagc cgctggatcc tgccgtccag ctcagccagg
     8941 aggaccccgg ccatcctgat cagtgaggtg gtcagatccc ttctagtggt ctcagacttg
     9001 ttcaccgagc gcaaccagtt tgagctgatg tatgtgacgc tgacagaact gcgaagggtg
     9061 cacccttcag aagacgagat cctcgctcag tacctggtgc ctgccacctg caaggcagct
     9121 gccgtccttg ggatggacaa ggccgtggcg gagcctgtca gccgcctgct ggagagcacg
     9181 ctcaggagca gccacctgcc cagcagggtt ggagccctgc acggcatcct ctatgtgctg
     9241 gagtgcgacc tgctggacga cactgccaag cagctcatcc cggtcatcag cgactatctc
     9301 ctctccaacc tgaaagggat cgcccactgc gtgaacattc acagccagca gcacgtactg
     9361 gtcatgtgtg ccactgcgtt ttacctcatt gagaactatc ctctggacgt agggccggaa
     9421 ttttcagcat caataataca gatgtgtggg gtgatgctgt ctggaagtga ggagtccacc
     9481 ccctccatca tttaccactg tgccctcaga ggcctggagc gcctcctgct ctctgagcag
     9541 ctctcccgcc tggatgcaga atcgctggtc aagctgagtg tggacagagt gaacgtgcac
     9601 agcccgcacc gggccatggc ggctctgggc ctgatgctca cctgcatgta cacaggaaag
     9661 gagaaagtca gtccgggtag aacttcagac cctaatcctg cagcccccga cagcgagtca
     9721 gtgattgttg ctatggagcg ggtatctgtt ctttttgata ggatcaggaa aggctttcct
     9781 tgtgaagcca gagtggtggc caggatcctg ccccagtttc tagacgactt cttcccaccc
     9841 caggacatca tgaacaaagt catcggagag tttctgtcca accagcagcc atacccccag
     9901 ttcatggcca ccgtggtgta taaggtgttt cagactctgc acagcaccgg gcagtcgtcc
     9961 atggtccggg actgggtcat gctgtccctc tccaacttca cgcagagggc cccggtcgcc
    10021 atggccacgt ggagcctctc ctgcttcttt gtcagcgcgt ccaccagccc gtgggtcgcg
    10081 gcgatcctcc cacatgtcat cagcaggatg ggcaagctgg agcaggtgga cgtgaacctt
    10141 ttctgcctgg tcgccacaga cttctacaga caccagatag aggaggagct cgaccgcagg
    10201 gccttccagt ctgtgcttga ggtggttgca gccccaggaa gcccatatca ccggctgctg
    10261 acttgtttac gaaatgtcca caaggtcacc acctgcggtg gaagcggtga ctacaaagac
    10321 gatgacgaca agtaaaacct ctacttccaa tcgcaccatc atcaccatca ccatcaccac
    10381 catgattaca aggatgacga cgataagtga ggcggccgcc aacttgatat caagcttatc
    10441 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
    10501 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
    10561 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
    10621 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
    10681 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
    10741 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
    10801 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
    10861 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
    10921 ctcaatccag cggaccttcc ttcccgcggg cgcccccctc ctcacggcga gcgaagcttg
    10981 tcgagaagta ctagaggatc tctagagcct gcagtctcga caagcttgtc gagaagtact
    11041 agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
    11101 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
    11161 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    11221 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    11281 ggatctgatc actgcttgag cctaggagat ccgaaccaga taagtgaaat ctagttccaa
    11341 actattttgt catttttaat tttcgtatta gcttacgacg ctacacccag ttcccatcta
    11401 ttttgtcact cttccctaaa taatccttaa aaactccatt tccacccctc ccagttccca
    11461 actattttgt ccgcccacag cggggcattt ttcttcctgt tatgttttta atcaaacatc
    11521 ctgccaactc catgtgacaa accgtcatct tcggctactt tttctctgtc acagaatgaa
    11581 aatttttctg tcatctcttc gttattaatg tttgtaattg actgaatatc aacgcttatt
    11641 tgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
    11701 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
    11761 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
    11821 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
    11881 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
    11941 gagtccacgt tcttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
    12001 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
    12061 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
    12121 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
    12181 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
    12241 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
    12301 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
    12361 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
    12421 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
    12481 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
    12541 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
    12601 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
    12661 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
    12721 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
    12781 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
    12841 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
    12901 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
    12961 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
    13021 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
    13081 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
    13141 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
    13201 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
    13261 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
    13321 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
    13381 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
    13441 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
    13501 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
    13561 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
    13621 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
    13681 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
    13741 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
    13801 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
    13861 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
    13921 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
    13981 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
    14041 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
    14101 catagaccag ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa tggatgccct
    14161 gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga tctaaactat
    14221 gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc agttatgctg
    14281 tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt ctgaagtgca
    14341 aattgcccgt cgtattaaag aggggcgtgg ccaagggcat ggtaaagact atattcgcgg
    14401 cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat
    14461 tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac
    14521 tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa
    14581 gcaccaagcg cgttggcctc atgcttcaag agattgatga gcgcggtggc aatgccctgc
    14641 ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca
    14701 aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca
    14761 agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt
    14821 tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg
    14881 gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct
    14941 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt
    15001 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
    15061 aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
    15121 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
    15181 ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca
    15241 cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gaggcatttc tgtcctggct
    15301 ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag gcattggcgg ccttgctgtt
    15361 cttctacggc aaggtgctgt gcacggatct gcccttgctt caggagatcg gtagacctcg
    15421 gccgtcgcgg cgcttgccgg tggtgctgac cccggatgaa gtggttcgca tcctcggttt
    15481 tctggaaggc gagcatcgtt tgttcgccca ggactctagc tatagttcta gtggttggct
    15541 acgtacccgt agtggctatg gcagggcttg ccgccccgac gttggctgcg agccctgggc
    15601 cttcacccga acttgggggt tggggtgggg aaaaggaaga aacgcgggcg tattggtccc
    15661 aatggggtct cggtggggta tcgacagagt gccagccctg ggaccgaacc ccgcgtttat
    15721 gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg
    15781 gttccttccg gtattgtctc cttccgtgtt tcagttagcc tcccccatct cccggtaccg
    15841 catgcggatc tgtacgggcc agatatacgc gtt
//
