LOCUS       sequence-272593-        3144 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for FLAG-tagged mGluR3 fused to the 
            N-terminal half of split fluorescent Venus (VN).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3144)
  TITLE     Mammalian expression plasmids for production of metabotropic 
            glutamate receptor type 3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCEP4_HLA-flag-GRM3 C127A-VN
FEATURES             Location/Qualifiers
     source          1..3144
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             91..114
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(2674..2676,2677..3135)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYLTA"
     primer_bind     complement(2716..2737)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2977..2996)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gctagcgcca ccatggccgt catggcgccc cgaaccctcg tcctgctact ctcgggggcc
       61 ctggccctga cccagacctg ggcgggctcc gactacaagg acgatgatga caagggagga
      121 tccaggagag agattaaaat agaaggtgac cttgttttag ggggcctgtt tcctattaac
      181 gaaaaaggca ctggaactga agaatgtggg cgaatcaatg aagaccgagg gattcaacgc
      241 ctggaagcca tgttgtttgc tattgatgaa atcaacaaag atgattactt gctaccagga
      301 gtgaagttgg gtgttcacat tttggataca tgttcaaggg atacctatgc attggagcaa
      361 tcactggagt ttgtcagggc atctttgaca aaagtggatg aagctgagta tatggctcct
      421 gatggatcct atgccattca agaaaacatc ccacttctca ttgcaggggt cattggtggc
      481 tcttatagca gtgtttccat acaggtggca aacctgctgc ggctcttcca gatccctcag
      541 atcagctacg catccaccag cgccaaactc agtgataagt cgcgctatga ttactttgcc
      601 aggaccgtgc cccccgactt ctaccaggcc aaagccatgg ctgagatctt gcgcttcttc
      661 aactggacct acgtgtccac agtagcctcc gagggtgatt acggggagac agggatcgag
      721 gccttcgagc aggaagcccg cctgcgcaac atctgcatcg ctacggcgga gaaggtgggc
      781 cgctccaaca tccgcaagtc ctacgacagc gtgatccgag aactgttgca gaagcccaac
      841 gcgcgcgtcg tggtcctctt catgcgcagc gacgactcgc gggagctcat tgcggccgcc
      901 agccgcgcca atgcctcctt cacctgggtg gccagcgacg gctggggcgc gcaggagagc
      961 atcatcaagg gcagcgagca tgtggcctac ggcgccatca ccctggagct ggcctcccag
     1021 cctgtccgcc agttcgaccg ctacttccag agcctcaacc cctacaacaa ccaccgcaac
     1081 ccctggttcc gggacttctg ggagcaaaag tttcagtgca gcctccagaa caaacgcaac
     1141 cacaggcgcg tctgcgacaa gcacctggcc atcgacagca gcaactacga gcaagagtcc
     1201 aagatcatgt ttgtggtgaa cgcggtgtat gccatggccc acgctttgca caaaatgcag
     1261 cgcaccctct gtcccaacac taccaagctt tgtgatgcta tgaagatcct ggatgggaag
     1321 aagttgtaca aggattactt gctgaaaatc aacttcacgg ctccattcaa cccaaataaa
     1381 gatgcagata gcatagtcaa gtttgacact tttggagatg gaatggggcg atacaacgtg
     1441 ttcaatttcc aaaatgtagg tggaaagtat tcctacttga aagttggtca ctgggcagaa
     1501 accttatcgc tagatgtcaa ctctatccac tggtcccgga actcagtccc cacttcccag
     1561 tgcagcgacc cctgtgcccc caatgaaatg aagaatatgc aaccagggga tgtctgctgc
     1621 tggatttgca tcccctgtga accgtacgaa tacctggctg atgagtttac ctgtatggat
     1681 tgtgggtctg gacagtggcc cactgcagac ctaactggat gctatgacct tcctgaggac
     1741 tacatcaggt gggaagacgc ctgggccatt ggcccagtca ccattgcctg tctgggtttt
     1801 atgtgtacat gcatggttgt aactgttttt atcaagcaca acaacacacc cttggtcaaa
     1861 gcatcgggcc gagaactctg ctacatctta ttgtttgggg ttggcctgtc atactgcatg
     1921 acattcttct tcattgccaa gccatcacca gtcatctgtg cattgcgccg actcgggctg
     1981 gggagttcct tcgctatctg ttactcagcc ctgctgacca agacaaactg cattgcccgc
     2041 atcttcgatg gggtcaagaa tggcgctcag aggccaaaat tcatcagccc cagttctcag
     2101 gttttcatct gcctgggtct gatcctggtg caaattgtga tggtgtctgt gtggctcatc
     2161 ctggaggccc caggcaccag gaggtatacc cttgcagaga agcgggaaac agtcatccta
     2221 aaatgcaatg tcaaagattc cagcatgttg atctctctta cctacgatgt gatcctggtg
     2281 atcttatgca ctgtgtacgc cttcaaaacg cggaagtgcc cagaaaattt caacgaagct
     2341 aagttcatag gttttaccat gtacaccacg tgcatcatct ggttggcctt cctccctata
     2401 ttttatgtga catcaagtga ctacagagtg cagacgacaa ccatgtgcat ctctgtcagc
     2461 ctgagtggct ttgtggtctt gggctgtttg tttgcaccca aggttcacat catcctgttt
     2521 caaccccaga agaatgttgt cacacacaga ctgcacctca acaggttcag tgtcagtgga
     2581 actgggacca catactctca gtcctctgca agcacgtatg tgccaacggt gtgcaatggg
     2641 cgggaagtcc tcgactccac cacctcatct ctggtgagca agggcgagga gctgttcacc
     2701 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     2761 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagct gatctgcacc
     2821 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgggcta cggcctgcag
     2881 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     2941 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     3001 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     3061 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     3121 gtctatctga ccgcctaact cgag
//
