LOCUS       sequence-27260-e         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3'LTR 
            insertion, Zeo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti X2 Zeo/pTER shLUC (w648-1)
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    50..68
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(201..225)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggggtttatc acgtaacagt gaagtctttg gatttgggat cttataagtt ccctatcagt
       61 gatagagatc cccttacgct gagtacttcg agtgtgctgt cctcgaagta ctcagcgtaa
      121 gtttttggaa aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgcg
      181 gccgcactcg agatatctag acccagcttt cttgtacaaa gtggttgata tccagcacag
      241 tggcggccgc tcgagtctag cccttccagt cccccctttt cttttaaaaa gtggctaaga
      301 tctacagctg ccttgtaagt cattggtctt aaaggtacca tttaaatctc gtagcacgtg
      361 tcagtcctgc tcctcggcca cgaagtgcac gcagttgccg gccgggtcgc gcagggcgaa
      421 ctcccgcccc cacggctgct cgccgatctc ggtcatggcc ggcccggagg cgtcccggaa
      481 gttcgtggac acgacctccg accactcggc gtacagctcg tccaggccgc gcacccacac
      541 ccaggccagg gtgttgtccg gcaccacctg gtcctggacc gcgctgatga acagggtcac
      601 gtcgtcccgg accacaccgg cgaagtcctc ctccacgaag tcccggcaga acccgagccg
      661 gtcggtccag aacccgaccg ctccggtgac gtcccgcgcg atgacgcacc cgaacggcac
//
