LOCUS       sequence-27261-e         629 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Gateway destination vector, RNAi, 3'LTR 
            insertion, Zeo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti X2 Zeo DEST (749-3)
FEATURES             Location/Qualifiers
     source          1..629
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        10..236
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             237..417
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    464..589
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 cagccttaat gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt
       61 agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt
      121 ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca
      181 ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt
      241 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
      301 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
      361 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
      421 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
      481 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
      541 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
      601 ggggagaatt agatcgcgag gggaaaaaa
//
