LOCUS       sequence-27262-e         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Peterson TR, Laplante M, Thoreen CC, Sancak Y, Kang SA, Kuehl WM, 
            Gray NS, Sabatini DM
  TITLE     DEPTOR is an mTOR inhibitor frequently overexpressed in multiple 
            myeloma cells and required for their survival.
  JOURNAL   Cell. 2009 May 29;137(5):873-86. doi: 10.1016/j.cell.2009.03.046. 
            Epub 2009 May 14.
  PUBMED    19446321
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2009.03.046"; journalName: "Cell"; date: 
            "2009-05-29- 29"; volume: "137"; issue: "5"; pages: "873-86"
COMMENT     Sequence Label: pRK5 FLAG DEPTOR (PDZ domain)
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..81
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      400..409
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    413..547
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(450..469)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     504..523
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(639..659)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 tccactccca ggtccaactg cacctcggtt ctatcgattg aattcaccat ggaaatggac
       61 tacaaggatg acgatgacaa gggtggtggt ggtggtgcgt cgacggctcc gtatgcaagg
      121 aagacattca cgattgttgg tgacgcggtt ggctggggtt ttgtggtgcg aggaagtaag
      181 ccatgccaca tccaggctgt agaccccagt ggccctgcag ccgcagcagg aatgaaggtc
      241 tgtcagtttg tcgtctctgt caacgggctc aatgtcctgc atgtagacta ccggaccgtg
      301 aacaatctga ttctgacggg cccacggacg attgtcatgg aagtcatgga ggagttagag
      361 tgctgagcgg ccgctaagta agtaaggatc cccagcttgg ccgccatggc ccaacttgtt
      421 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
      481 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
      541 ctggatcggg aattaattcg gcgcagcacc atggcctgaa ataacctctg aaagaggaac
      601 ttggttaggt accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
      661 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agc
//
