LOCUS       sequence-272635-        9081 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  encodes Kir2.1 potassium channel for lentiviral transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9081)
  AUTHORS   McNamara HM, Salegame R, Al Tanoury Z, Xu H, Begum S, Ortiz G, 
            Pourquie O, Cohen AE
  TITLE     Bioelectrical domain walls in homogeneous tissues.
  JOURNAL   Nat Phys. 2020 Mar;16(3):357-364. doi: 10.1038/s41567-019-0765-4. 
            Epub 2020 Jan 20.
  PUBMED    33790984
REFERENCE   2  (bases 1 to 9081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41567-019-0765-4"; journalName: "Nat Phys"; date: 
            "2020-03"; volume: "16"; issue: "3"; pages: "357-364"
COMMENT     Sequence Label: HK_13_Bla_Lenti_Kir2.1
FEATURES             Location/Qualifiers
     source          1..9081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1483..1983
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1902..1920
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1995..2390
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    2450..2567
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2684..2987
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2988..3191
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3142..3162
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    3214..3238
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    3280..3300
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      3300..3309
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(4598..4622)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4624..4665
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4707..5295
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4760..4780)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5178..5189)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5367..5600
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5678..5799
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5715..5734)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5769..5788
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5839..5974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5994..6013)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5995..6013)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6023..6040)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6023..6039)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6032..6054)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6181..6636
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6268..6287)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6478..6499
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6662..6766
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6767..6835,6836..7627)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6985..7004)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7798..8386
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8287..8306
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8540..8557
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8674..8695
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8710..8715,8716..8733,8734..8740)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8748..8764
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8753..8775
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8772..8788
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8772..8788
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8807..8827
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8809..8827
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8855..9081
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccttgcgcct tttccaaggc
     1501 agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc
     1561 gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc
     1621 gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc
     1681 ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc
     1741 gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat
     1801 agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg
     1861 gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag
     1921 cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc
     1981 cagggggtac caccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag
     2041 caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc
     2101 tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt
     2161 gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta
     2221 tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg
     2281 tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga
     2341 cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc ctgcagctgc
     2401 agtagtaaga attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
     2461 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     2521 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     2581 gggacagcag agatccactt tggcgccggc tcgagggggc ccggggttat taatagtaat
     2641 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     2701 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     2761 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     2821 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     2881 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     2941 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     3001 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     3061 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     3121 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     3181 taagcagagc tctctggcta actgtcggga tcaacaagtt tgtacaaaaa agcaggctgg
     3241 cgccggaacc aattcagtcg actggatccg ccaccatgcc aactttgtat acaaaagttg
     3301 ccgccatggg cagtgtgaga accaaccgct acagcatcgt ctcttcggag gaagatggca
     3361 tgaagctggc cactatggca gttgccaatg gctttgggaa tggcaagagt aaagtccata
     3421 cccgacaaca gtgcaggagc cgctttgtga agaaagatgg tcattgcaat gttcagttta
     3481 tcaacgtggg tgagaaggga cagaggtacc tggcagacat ctttactacc tgtgtcgaca
     3541 tccgctggag gtggatgctg gttatcttct gtcttgcctt cgtgctctcc tggctgttct
     3601 ttggctgtgt gttttggttg atagccctgc tccatgggga tctagatact tctaaagtga
     3661 gcaaagcatg cgtgtcagag gtcaacagct tcacggctgc cttcctcttc tccatcgaga
     3721 cccagacaac cattggctat ggtttcaggt gtgtgacaga cgagtgccca attgctgtct
     3781 tcatggtggt attccagtca atcgtaggct gcatcattga cgccttcatc attggtgcag
     3841 tcatggcgaa gatggcaaag ccaaagaaga gaaatgagac tcttgtcttc agtcacaatg
     3901 ctgtgattgc catgagggat ggcaaactct gcttgatgtg gagagtgggt aaccttcgaa
     3961 agagccacct tgtggaagct catgtccggg cacagcttct caaatctagg atcacttcag
     4021 aaggggagta tatccctttg gaccagatag acatcaatgt tggttttgat agtggaattg
     4081 accgcatatt tctagtgtcc cccatcacta tcgttcacga aatagatgaa gacagccctt
     4141 tatatgactt gagtaagcag gacattgaca atgcagactt tgaaattgtt gtcatactgg
     4201 aaggcatggt ggaggcgact gccatgacaa ctcaatgccg gagttcgtat ctggccaatg
     4261 aaattctctg gggtcaccgc tatgagccag tgctctttga agagaaacac tactataaag
     4321 tagactattc aagattccat aagacttatg aagtacctaa cacccccctt tgtagtgcca
     4381 gagacttagc agagaagaaa tacatccttt caaatgcaaa ttcattttgc tatgaaaatg
     4441 aagttgccct aacaagcaaa gaggaagagg aggatagtga gaacggagtc ccagagagca
     4501 caagcacaga ctcacctcct ggcatagatc tccacaacca ggcaagcgta cctctagagc
     4561 ccaggccctt aaggcgagaa tcggagatat gatctagacc cagctttctt gtacaaagtg
     4621 gttggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc
     4681 gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     4741 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     4801 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     4861 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     4921 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     4981 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     5041 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     5101 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     5161 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     5221 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     5281 gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag
     5341 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     5401 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     5461 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     5521 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     5581 agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt
     5641 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     5701 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5761 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     5821 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5881 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     5941 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     6001 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     6061 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     6121 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     6181 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6241 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6301 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6361 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6421 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6481 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6541 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6601 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     6661 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6721 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6781 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6841 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6901 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6961 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7021 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7081 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7141 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7201 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7261 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7321 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7381 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7441 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7501 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7561 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7621 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7681 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7741 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7801 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7861 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7921 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     7981 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8041 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8101 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8161 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8221 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8281 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8341 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8401 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8461 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8521 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     8581 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     8641 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     8701 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     8761 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     8821 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
     8881 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
     8941 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
     9001 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
     9061 tgcctagctc gatacataaa c
//
