LOCUS       sequence-272639-        7572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-terminal myc-tagged mouse KIF5C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7572)
  AUTHORS   Twelvetrees AE, Yuen EY, Arancibia-Carcamo IL, MacAskill AF, 
            Rostaing P, Lumb MJ, Humbert S, Triller A, Saudou F, Yan Z, Kittler 
            JT
  TITLE     Delivery of GABAARs to synapses is mediated by HAP1-KIF5 and 
            disrupted by mutant huntingtin.
  JOURNAL   Neuron. 2010 Jan 14;65(1):53-65. doi: 10.1016/j.neuron.2009.12.007.
  PUBMED    20152113
REFERENCE   2  (bases 1 to 7572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2009.12"; journalName: "Neuron"; date: 
            "2010-01-14- 14"; volume: "65"; issue: "1"; pages: "53-65"
COMMENT     Sequence Label: myc-KIF5C
FEATURES             Location/Qualifiers
     source          1..7572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     815..832
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     846..865
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      912..921
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             921..950
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      3859..3868
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3872..4006
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3909..3928)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3963..3982
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        4075..4432
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4075..4266
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4098..4118)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4283..4418
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4452..4469)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4452..4468)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4461..4483)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4681..5136
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4768..4787)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4978..4999
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5153..5172)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5272..5294
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5332..5350)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5418..5522
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5523..5591,5592..6383)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5741..5760)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6554..7142
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7043..7062
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7296..7313
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7430..7451
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7466..7471,7472..7489,7490..7496)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7504..7520
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7509..7531
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7528..7544
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7528..7544
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
      901 cgattgaatt ggccaccatg gagcagaagc tgatctccga ggaggacctg ggatcccggg
      961 gcggccgctc tagaattcct atggcggatc cagccgaatg cagcatcaaa gtgatgtgcc
     1021 ggttccggcc cctcaacgaa gcggagatcc tccgcgggga caaattcatc cccaaattca
     1081 aaggcgagga gacggtggtg atcgggcaag ggaagccgta tgtctttgac cgagtgctgc
     1141 cgcccaacac aacccaggag caggtctaca atgcctgtgc aaagcagatt gtcaaagatg
     1201 tccttgaggg ttataatgga acaatttttg catatgggca gacttcatca ggaaaaactc
     1261 ataccatgga ggggaagtta catgatcctc agcttatggg tatcattcca aggattgcac
     1321 atgatatttt tgatcacatc tattccatgg acgagaacct ggagtttcat atcaaggttt
     1381 cctattttga gatctacttg gacaaaataa gggacttgct tgatgtgtcc aagaccaact
     1441 tggcagttca tgaagacaaa aacagagtcc cctatgtaaa ggggtgcacc gagaggtttg
     1501 tgtcaagccc ggaggaggtc atggatgtga tcgatgaggg caaagcaaac cgacacgtgg
     1561 ctgtgacaaa catgaacgaa cacagctcta ggagtcacag tatcttcctg attaacatta
     1621 agcaagagaa tgtggagact gaaaaaaaac tcagcgggaa gctgtatttg gttgatttgg
     1681 ctgggagtga aaaggtcagc aaaaccggtg ccgagggagc tgttcttgat gaagctaaaa
     1741 atatcaacaa gtctttgtct gctcttggaa atgtgatttc tgccttggca gaagggacaa
     1801 aaacacatgt accgtaccgg gacagcaaga tgactcggat tctccaggac tctctgggtg
     1861 ggaactgtag gaccaccatt gtcatttgct gttctccttc agtcttcaat gaagccgaga
     1921 ccaagtccac gctgatgttt ggacagagag caaagaccat caagaataca gtctctgtga
     1981 acttggaact aacagcagaa gagtggaaga agaaatatga aaaagagaaa gagaagaaca
     2041 aggccttgaa gagtgtcctc cagcatctgg agatggagct gaacaggtgg aggaacgggg
     2101 aagctgtacc cgaggacgaa cagatcagcg ccaaggacca gaagagccta gagccctgtg
     2161 acaacacacc catcatagac aacatcacgc ctgttgtgga cggcatctct gccgagaagg
     2221 agaagtatga cgaggagatc accagtttgt accgacagct cgatgataag gatgatgaaa
     2281 ttaaccagca gagccagctg gctgaaaagc tgaagcaaca gatgttggat caggatgagc
     2341 tcctggcttc cacgagaagg gactatgaga agatccagga ggagctgaca cgcctccaga
     2401 tcgaaaatga ggcagctaaa gacgaagtga aagaagtcct ccaggccctg gaggagctgg
     2461 ctgtcaatta cgaccagaag tcacaagaag tggaggacaa gaccagggcc aacgagcaac
     2521 tgactgatga gctggcccag aaaacgacga cactgacaac cacccagcga gagctgagtc
     2581 agctgcaaga gcttagtaac caccagaaaa agagggccac agagatcctg aacctgcttc
     2641 tcaaggacct gggggagata ggcggaatta ttggcaccaa cgatgtgaag actctggcag
     2701 atgtgaacgg ggtcattgag gaggagttca ccatggcacg cctgtacatt agcaagatga
     2761 agtcggaggt caagtctctc gtgaaccgca gcaagcagct ggagagtgcc cagatggact
     2821 ctaacaggaa gatgaacgcc agtgagcgcg agctggcagc gtgccagttg cttatctcac
     2881 agcacgaagc caagatcaag tctctgacag actacatgca gaacatggaa cagaagaggc
     2941 ggcagctgga agagtcccag gactccctca gcgaggaact ggccaagctc cgggcccagg
     3001 aaaaaatgca cgaagtcagt ttccaagata aggaaaagga gcacctgacg aggctgcagg
     3061 atgctgagga ggtgaagaaa gctctggagc agcagatgga gagccaccgg gaagcgcacc
     3121 aaaagcagct gtccagactt cgcgatgaga ttgaggagaa gcagagaatc attgatgaga
     3181 tccgggattt gaatcagaaa ctgcaactgg aacaggagag gctcagctct gattataaca
     3241 agctgaaaat agaggaccag gagagagaag tgaaactgga gaagctccta ttgctcaatg
     3301 acaaaaggga gcaagccagg gaggacctca agggactgga ggagactgtg tctagagaac
     3361 tccagaccct tcataacctg cgcaaactct tcgtccagga tttgacaacc cgggtgaaaa
     3421 agagtgtgga gctggacagc gacgatggag ggggcagcgc tgctcagaag cagaagatct
     3481 ccttcctgga gaacaacctg gaacagctta ccaaggtgca caagcagctg gtccgggaca
     3541 atgcagattt gcgctgtgaa ctccccaagc tggagaagag gcttcgtgct accgcagaac
     3601 gcgtcaaggc cttggagagt gcgctgaaag aggccaagga gaatgccatg agggaccgaa
     3661 aacgctacca gcaggaagta gatcgcatca aggaggctgt gcgagccaag aacatggcca
     3721 ggagggcaca ttcggctcag atcgccaagc ccatccgccc aggccattac ccagcatcat
     3781 ctccgacagc tgtccatgcc gtccgaggag gaggaggtgg ctcttcaaac tctactcact
     3841 accagaaata aaagcttggc cgccatggcc caacttgttt attgcagctt ataatggtta
     3901 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     3961 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcggga attaattcgg
     4021 cgcagcacca tggcctgaaa taacctctga aagaggaact tggttaggta ccttctgagg
     4081 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     4141 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     4201 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     4261 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     4321 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     4381 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctgtt
     4441 aacagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     4501 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     4561 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
     4621 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt
     4681 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4741 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4801 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     4861 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     4921 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4981 gactcttgtt ccaaactgga acaacactca accctatctc gggctattct tttgatttat
     5041 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     5101 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
     5161 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     5221 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     5281 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     5341 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     5401 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5461 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5521 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     5581 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     5641 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5701 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5761 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5821 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5881 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5941 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     6001 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     6061 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     6121 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     6181 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     6241 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     6301 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     6361 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     6421 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6481 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6541 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6601 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6661 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6721 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6781 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6841 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6901 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6961 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     7021 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7081 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7141 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     7201 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     7261 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     7321 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     7381 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     7441 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     7501 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaattaat
     7561 tcgagctcgc cc
//
