LOCUS       sequence-272640-        9681 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  WT UPF1 inserted with lambdaN tagged on the N-terminus to use with 
            the Tethering Assay (BoxB).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Fischer JW, Busa VF, Shao Y, Leung AKL
  TITLE     Structure-Mediated RNA Decay by UPF1 and G3BP1.
  JOURNAL   Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi: 
            10.1016/j.molcel.2020.01.021. Epub 2020 Feb 3.
  PUBMED    32017897
REFERENCE   2  (bases 1 to 9681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01.021"; journalName: "Mol Cell"; date: 
            "2020-04-2- 2"; volume: "78"; issue: "1"; pages: "70-84.e6"
COMMENT     Sequence Label: pCI-neo-lambdaN-UPF1-WT
FEATURES             Location/Qualifiers
     source          1..9681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        80..459
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        460..663
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     614..634
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     660..684
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          824..956
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     939..958
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1001..1019
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    4534..4655
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4545..4564)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4599..4618
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      4841..5296
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5413..5770
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5413..5604
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5436..5456)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5621..5756
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5821..6615
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5875..5894)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6485..6504
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6679..6727
                     /note="synthetic polyadenylation signal"
     promoter        complement(6803..6821)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6835..6851)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6835..6851)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6848..6870)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6859..6875
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6883..6889,6890..6907,6908..6913))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6928..6949
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7066..7083)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7237..7825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7317..7336)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7996..8787,8788..8856))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8619..8638
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8857..8961)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8988..9443
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     9569..9591
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9583..9600
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9584..9600
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tattggccat tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt
       61 ccaatatgac cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg
      121 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
      181 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      241 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
      301 gcccacttgg cagtacatca agtgtatcat atgccaagtc cgccccctat tgacgtcaat
      361 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttacggga ctttcctact
      421 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
      481 accaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
      541 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaataac
      601 cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
      661 gctcgtttag tgaaccgtca gatcactaga agctttattg cggtagttta tcacagttaa
      721 attgctaacg cagtcagtgc ttctgacaca acagtctcga acttaagctg cagtgactct
      781 cttaaggtag ccttgcagaa gttggtcgtg aggcactggg caggtaagta tcaaggttac
      841 aagacaggtt taaggagacc aatagaaact gggcttgtcg agacagagaa gactcttgcg
      901 tttctgatag gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgt
      961 ccactcccag ttcaattaca gctcttaagg ctagagtact taatacgact cactataggc
     1021 tagccaccat gaacgcacga acacgacgac gtgagcgtcg cgctgagaaa caagctcaat
     1081 ggaaagctgc aaacgcttag cgtggaggcg tacgggccca gctcgcagac tctcactttc
     1141 ctggacacgg aggaggccga gctgcttggc gccgacacac agggctccga gttcgagttc
     1201 accgacttta ctcttcctag ccagacgcag acgccccccg gcggccccgg cggcccgggc
     1261 ggtggcggcg cgggaggccc gggcggcgcg ggcgcgggcg ctgcggcggg acagctcgac
     1321 gcgcaggttg ggcccgaagg catcctgcag aacggggctg tggacgacag tgtagccaag
     1381 accagccagt tgttggctga gttgaacttc gaggaagatg aagaagacac ctattacacg
     1441 aaggacctcc ccatacacgc ctgcagttac tgtggaatac acgatcctgc ctgcgtggtt
     1501 tactgtaata ccagcaagaa gtggttctgc aacggacgtg gaaatacttc tggcagccac
     1561 attgtaaatc accttgtgag ggcaaaatgc aaagaggtga ccctgcacaa ggacgggccc
     1621 ctgggggaga cagtcctgga gtgctacaac tgcggctgtc gcaacgtctt cctcctcggc
     1681 ttcatcccgg ccaaagctga ctcagtggtg gtgctgctgt gcaggcagcc ctgtgccagc
     1741 cagagcagcc tcaaggacat caactgggac agctcgcagt ggcagccgct gatccaggac
     1801 cgctgcttcc tgtcctggct ggtcaagatc ccctccgagc aggagcagct gcgggcacgc
     1861 cagatcacgg cacagcagat caacaagctg gaggagctgt ggaaggaaaa cccttctgcc
     1921 acgctggagg acctggagaa gccgggggtg gacgaggagc cgcagcatgt cctcctgcgg
     1981 tacgaggacg cctaccagta ccagaacata ttcgggcccc tggtcaagct ggaggccgac
     2041 tacgacaaga agctgaagga gtcccagact caagataaca tcactgtcag gtgggacctg
     2101 ggccttaaca agaagagaat cgcctacttc actttgccca agactgactc tgacatgcgg
     2161 ctcatgcagg gggatgagat atgcctgcgg tacaaagggg accttgcgcc cctgtggaaa
     2221 gggatcggcc acgtcatcaa ggtccctgat aattatggcg atgagatcgc cattgagctg
     2281 cggagcagcg tgggtgcacc tgtggaggtg actcacaact tccaggtgga ttttgtgtgg
     2341 aagtcgacct cctttgacag gatgcagagc gcattgaaaa cgtttgccgt ggatgagacc
     2401 tcggtgtctg gctacatcta ccacaagctg ttgggccacg aggtggagga cgtaatcatc
     2461 aagtgccagc tgcccaagcg cttcacggcg cagggcctcc ccgacctcaa ccactcccag
     2521 gtttatgccg tgaagactgt gctgcaaaga ccactgagcc tgatccaggg cccgccaggc
     2581 acggggaaga cggtgacgtc ggccaccatc gtctaccacc tggcccggca aggcaacggg
     2641 ccggtgctgg tgtgtgctcc gagcaacatc gccgtggacc agctaacgga gaagatccac
     2701 cagacggggc taaaggtcgt gcgcctctgc gccaagagcc gtgaggccat cgactccccg
     2761 gtgtcttttc tggccctgca caaccagatc aggaacatgg acagcatgcc tgagctgcag
     2821 aagctgcagc agctgaaaga cgagactggg gagctgtcgt ctgccgacga gaagcggtac
     2881 cgggccttga agcgcaccgc agagagagag ctgctgatga acgcagatgt catctgctgc
     2941 acatgtgtgg gcgccggtga cccgaggctg gccaagatgc agttccgctc cattttaatc
     3001 gacgaaagca cccaggccac cgagccggag tgcatggttc ccgtggtcct cggggccaag
     3061 cagctgatcc ttgtaggcga ccactgccag ctgggcccag tggtgatgtg caagaaggcg
     3121 gccaaggccg ggctgtcaca gtcgctcttc gagcgcctgg tggtgctggg catccggccc
     3181 atccgcctgc aggtccagta ccggatgcac cctgcactca gcgccttccc atccaacatc
     3241 ttctacgagg gctccctcca gaatggtgtc actgcagcgg atcgtgtgaa gaagggattt
     3301 gacttccagt ggccccaacc cgataaaccg atgttcttct acgtgaccca gggccaagag
     3361 gagattgcca gctcgggcac ctcctacctg aacaggaccg aggctgcgaa cgtggagaag
     3421 atcaccacga agttgctgaa ggcaggcgcc aagccggacc agattggcat catcacgccc
     3481 tacgagggcc agcgctccta cctggtgcag tacatgcagt tcagcggctc cctgcacacc
     3541 aagctctacc aggaggtgga gatcgccagt gtggacgcct ttcagggacg cgagaaggac
     3601 ttcatcatcc tgtcctgtgt gcgggccaac gagcaccaag gcattggctt tttaaatgac
     3661 cccaggcgtc tgaacgtggc cctgaccaga gcaaggtatg gcgtcatcat tgtgggcaac
     3721 ccgaaggcac tatcaaagca gccgctctgg aaccacctgc tgaactacta taaggagcag
     3781 aaggtgctgg tggaggggcc gctcaacaac ctgcgtgaga gcctcatgca gttcagcaag
     3841 ccacggaagc tggtcaacac tatcaacccg ggagcccgct tcatgaccac agccatgtat
     3901 gatgcccggg aggccatcat cccaggctcc gtctatgatc ggagcagcca gggccggcct
     3961 tccagcatgt acttccagac ccatgaccag attggcatga tcagtgccgg ccctagccac
     4021 gtggctgcca tgaacattcc catccccttc aacctggtca tgccacccat gccaccgcct
     4081 ggctattttg gacaagccaa cgggcctgct gcagggcgag gcaccccgaa aggcaagact
     4141 ggtcgtgggg gacgccagaa gaaccgcttt gggcttcctg gacccagcca gactaacctc
     4201 cccaacagcc aagccagcca ggatgtggcg tcacagccct tctctcaggg cgccctgacg
     4261 cagggctaca tctccatgag ccagccttcc cagatgagcc agcccggcct ctcccagccg
     4321 gagctgtccc aggacagtta ccttggtgac gagtttaaat cacaaatcga cgtggcgctc
     4381 tcacaggact ccacgtacca gggagagcgg gcttaccagc atggcggggt gacggggctg
     4441 tcccagtatt aaaaggtggc ggcggaagag ctaaagagtc gacccgggcg gccgcttccc
     4501 tttagtgagg gttaatgctt cgagcagaca tgataagata cattgatgag tttggacaaa
     4561 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     4621 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     4681 tgtttcaggt tcagggggag atgtgggagg ttttttaaag caagtaaaac ctctacaaat
     4741 gtggtaaaat ccgataagga tcgatccggg ctggcgtaat agcgaagagg cccgcaccga
     4801 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca
     4861 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     4921 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     4981 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5041 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5101 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5161 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     5221 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     5281 ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     5341 accgcatacg cggatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg
     5401 gttaggtacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     5461 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     5521 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     5581 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     5641 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     5701 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5761 cttttgcaaa aagcttgatt cttctgacac aacagtctcg aacttaaggc tagagccacc
     5821 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     5881 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     5941 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     6001 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     6061 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     6121 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     6181 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     6241 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     6301 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     6361 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     6421 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     6481 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     6541 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     6601 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     6661 tgccatcacg atggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt
     6721 ttgtgtgaat cgatagcgat aaggatcccc taggcttttg caaaaagctc ctcgaggaac
     6781 tgaaaaacca gaaagttaat tccctatagt gagtcgtatt aaattcgtaa tcatgtcata
     6841 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6901 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6961 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7021 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7081 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7141 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7201 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7261 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7321 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7381 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7441 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7501 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7561 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7621 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7681 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7741 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7801 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7861 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7921 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7981 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8041 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8101 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8161 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8221 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8281 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8341 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8401 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8461 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8521 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8581 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     8641 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8701 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8761 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8821 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8881 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8941 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag
     9001 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     9061 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     9121 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     9181 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     9241 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     9301 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     9361 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     9421 caaaatatta acgcttacaa tttccattcg ccattcaggc tgcgcaactg ttgggaaggg
     9481 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     9541 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     9601 gccaagctga tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat
     9661 atagcataaa tcaatattgg c
//
