LOCUS       sequence-272645-       10803 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of CENP-C and KNL2 CDK SA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10803)
  AUTHORS   McKinley KL, Sekulic N, Guo LY, Tsinman T, Black BE, Cheeseman IM
  TITLE     The CENP-L-N Complex Forms a Critical Node in an Integrated Meshwork
            of Interactions at the Centromere-Kinetochore Interface.
  JOURNAL   Mol Cell. 2015 Dec 17;60(6):886-98. doi: 
            10.1016/j.molcel.2015.10.027. Epub 2015 Nov 19.
  PUBMED    26698661
REFERENCE   2  (bases 1 to 10803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.10.027"; journalName: "Mol Cell"; date: 
            "2015-12-17- 17"; volume: "60"; issue: "6"; pages: "886-98"
COMMENT     Sequence Label: pKM296
FEATURES             Location/Qualifiers
     source          1..10803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        69..448
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        449..652
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     603..623
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    654..672
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    675..693
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     703..727
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      816..825
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             822..1529
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(967..985)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1235..1254
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1575..1595
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1608..1652
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(6589..6606)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6595..6819
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6952..6971)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7103..7150
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7158..8180
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8310..8431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8347..8366)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8401..8420
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8480..8496)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8480..8496)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8493..8515)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8504..8520
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8528..8534,8535..8552,8553..8558))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8573..8594
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8711..8728)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8882..9470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8962..8981)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9641..10432,10433..10501))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10264..10283
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10502..10606)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10681..10700)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
       61 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
      121 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      181 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      241 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      301 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      361 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      421 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
      481 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      541 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      601 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctccctat
      661 cagtgataga gatctcccta tcagtgatag agatcgtcga cgagctcgtt tagtgaaccg
      721 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg
      781 atccagcctc cggactctag cgtttgctac cggtcgccac catggtgagc aagggcgagg
      841 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
      901 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
      961 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
     1021 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     1081 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     1141 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
     1201 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
     1261 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     1321 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
     1381 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
     1441 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
     1501 ccaccggcgg catggacgag ctgtacaagt ccggagggga tcggtggagc tccaccggtg
     1561 gcggccgctc tagagagaat ctttattttc agggcgccgc caaattcaaa gaaaccgctg
     1621 ctgctaaatt cgaacgccag cacatggaca gcggaggtgg aggtactagt ctcgaggaag
     1681 aagtgacttc aaccgttacc aaaagccgcc gtattagtcg tcgtcctagc gattggtggg
     1741 ttgtgaaaag cgaagaaagc ccggtgtata gcaattctag cgtccgtaat gagctgccta
     1801 tgcaccataa cagttcccgt aaaagcacca aaaaaaccaa ccagtctagc aaaaacattc
     1861 gtaaaaaaac gatccctctg aaacgccaga aaaccgccac aaaaggcaac caacgtgttc
     1921 agaaattcct gaacgccgaa ggatcaggag gcattgttgg gcacgatgag attagtcgct
     1981 gctccctgtc agaacctctg gaatccgatg aagccgatct ggctaaaaaa aaaaacctgg
     2041 actgctcccg ttctacccgt agcagtaaaa atgaggataa catcatgacc gcccagaatg
     2101 tcccactgaa acctcagaca agcggttata cctgtaacat tccgaccgaa agcaatctgg
     2161 acagcggtga gcacaaaaca agtgtcctgg aagagtctgg tcctagtcgt ctgaataaca
     2221 attatctgat gtccgggaaa aatgatgtgg acgatgagga agttcacggt agtagcgacg
     2281 attcaaaaca gagtaaagtc atccctaaaa atcgtattca ccataaactg gtgctgccga
     2341 gtaatactcc gaacgttcgt cgtacaaaac gtacacgcct gaaacctctg gaatattggc
     2401 gtggtgaacg tatcgactac cagggtcgtc catctggtgg ttttgtaatc agcggtgtgc
     2461 tgagtcctga tacgattagc agcaaacgta aagccaaaga gaacatcggc aaagtcaaca
     2521 aaaaatccaa caaaaaacgc atctgcctgg ataacgatga gcgtaaaacg aatctgatgg
     2581 tgaatctggg tattcctctg ggagatcctc tgcagcctac ccgtgtaaaa gatcctgaga
     2641 cacgtgagat tatcctgatg gacctggttc gtcctcaaga cacctatcag tttttcgtca
     2701 aacacggcga gctgaaagtc tataaaaccc tggacacccc gttctttagt accggcaaac
     2761 tgattctggg tcctcaggag gaaaaaggca aacagcacgt gggtcaagac atcctggtct
     2821 tttatgtgaa ttttggggac ctgctgtgta ctctgcatga aaccccgtat attctgtcta
     2881 ccggtgacag cttctatgtg ccgtctggaa actattataa catcaaaaac ctgcgcaacg
     2941 aagaaagtgt tctgctgttc acacagatca aacgcggttc tggctccgga tcagggtcgg
     3001 gcagtggcag cggttcgggc tcgatgattg cagccccttt gaaacattca agaatttact
     3061 tacctccaga ggcatcttct caaaggagaa atctacccat ggatgcaatc ttttttgaca
     3121 gcattccttc aggcacactt gcccctgtaa aagatttggt gaaatatcag aactcctcct
     3181 taaaattgaa tgaccataaa aagaatcagt tcctaaaaat gacaactttt aacaataaaa
     3241 atatatttca atcaactatg ctaacagagg ctactacctc taacagttct cttgatatca
     3301 gtgctataaa gcccaacaag gatggattaa aaaataaagc aaactatgaa gccccaggaa
     3361 aaatatttct aagaatgaaa gaaaaagtac tgcgtgacaa gcaagaacag ccatcaagaa
     3421 acagtagttt gttggaacca cagaaaagtg gaaataatga aaccttcgcc cctaacagag
     3481 ttgaaaaaaa aaaattgcag catacctacc tatgtgaaga aaaggaaaac aacaaatcat
     3541 tccagtcaga tgacagttca ctaagagcct cagtccaagg agttcctcta gaatcatcaa
     3601 ataatgatat tttcctcccg gtcaaacaaa agattcagtg ccagcaggaa aagaaagcac
     3661 cactgcacaa tttaacttac gaacttccaa ctctgaacca agaacaggaa aattttttgg
     3721 ctgtagaagc ccgaaacaag acattaacta gagctcagtt ggctaaacaa atttttcact
     3781 caaaggagag tatagttgca accactaaat ccaaaaagga cacgtttgtt ttagaaagcg
     3841 ttgattctgc tgatgaacaa tttcaaaata ctaatgctga gactctcagt actaattgta
     3901 ttcctattaa aaatggcagc ctgttaatgg tttctgatag tgagaggaca acagaaggga
     3961 cttcgcaaca gaaagttaag gaaggaaatg gaaaaacagt gcctggagag acaggtcttc
     4021 caggttccat gaaagataca tgtaaaattg tacttgcagc cccaagactt catataacaa
     4081 tacctcggag gtcaaaaaga aatatttcaa agcttgcccc tccaagaata tttcaaactg
     4141 ttacaaatgg acttaaaaaa aatcaggtag ttcagctaca ggaatggatg attaaaagca
     4201 tcaataataa tactgctata tgtgtagaag gaaaattgat agacgtcact aacatatatt
     4261 ggcacagtaa tgtaattata gagcggattg agcacaacaa acttaggact atatcaggca
     4321 acgtttatat attaaaaggc atgatagacc aaatttccat gaaagaagca ggatatccaa
     4381 attatctcat aaggaaattt atgtttggat ttccagaaaa ttggaaagag cacattgata
     4441 attttctgga acaattaagg gctggtgaaa agaacaggga aaagaccaaa caaaaacaga
     4501 aaactggaag atctgtccgt gacataagga aatcaatgaa aaatgatgca cgagaaaacc
     4561 aaacagatac tgctcaaaga gccaccacca cttacgattt tgattgtgat aatttggaac
     4621 tgaagagtaa taagcacagt gaggccccag gagctacaga attaaacatg tgccacagta
     4681 attgccaaaa taaaccaaca ttaaggttcc cagatgacca agtaaataat actattcaaa
     4741 atggaggagg agatgactta tctaatcagg aattaattgg aaaaaaagaa tataaaatgt
     4801 cttcaaagaa actaaaaatt ggtgaaagaa caaatgaaag gataataaaa agtcagaagc
     4861 aagagacaac tgaagaattg gatgtatcca ttgatattct aacctcaagg gaacagtttt
     4921 tctcagatga agaaagaaaa tacatggcca tcaatcagaa gaaagcttat attttagtag
     4981 ccccacttaa atctagaaaa gtgatagagc aaagatgcat gaggtataat ctgtccgctg
     5041 gcaccatcaa agcagtaaca gattttgtaa taccagagtg tcaaaaaaaa gcccccatca
     5101 gcaagtccat ggggacttta gaaaatacat ttgaaggtca taaaagtaaa aacaaggaag
     5161 attgcgatga acgtgactta cttactgtca accggaaaat aaaaatatct aaccttgaaa
     5221 aggaacaaat gctcacctct gactttaaga aaaataccag actattacca aaattgaaga
     5281 aaatagaaaa tcaggtagct atgtcatttt ataagcatca gtccgcccca gatttgtcaa
     5341 gtgaagaaag tgaaacagaa aaggaaatta aaaggaaagc tgaagttaag aaaaccaaag
     5401 caggaaacac caaagaagca gtggttcacc tgagaaagag cacaagaaac acaagtaata
     5461 ttccagtgat tttggaacct gaaactgaag aaagtgaaaa tgaattttat atcaaacaaa
     5521 agaaagctag accttccgtc aaagaaactc ttcaaaaaag cggcgtgcgc aaggagtttc
     5581 caattactga ggcagtagga tctgataaga caaataggca tcccttagaa tgcttacctg
     5641 gtttaattca ggataaggaa tggaatgaga aggagttaca gaaacttcat tgtgcttttg
     5701 catctcttcc aaagcacaaa cctggtttct ggtcagaggt agctgcggct gtaggttctc
     5761 gggcccctga agaatgccag aggaaataca tggaaaatcc cagaggaaaa ggatcccaga
     5821 aacatgtcac taagaagaag ccagccaatt ccaaaggcca aaatggcaag agaggtgatg
     5881 ctgatcagaa acaaactatt aagataactg ccaaagtggg aactcttaaa aggaagcaac
     5941 agatgaggga atttctggaa cagttgccaa aagatgacca tgatgatttt ttcagtacag
     6001 cccctttaca gcatcaaaga atactgttgc caagtttcca ggacagtgaa gatgatgatg
     6061 atattctgcc aaatatggac aaaaatccaa cagccccatc atcagttatc tttccattgg
     6121 taaaagcccc tcaatgtcag catgtcgccc ctggcatgct aggttctata aataggaatg
     6181 actgtgataa atatgttttt cgtatgcaaa aatatcataa aagtaatggt ggtattgtct
     6241 ggggcaacat caagaaaaaa ttagttgaaa ctgatttctc agccccagcc ccaagaagga
     6301 aagccccatt taacacagac ttaggagaaa actctggtat tggaaaactt ttcactaatg
     6361 ctgtggaatc tttagatgaa gaagagaaag attattattt ttcgaactct gattctgcat
     6421 aggaattctg cagtcgacgg taccgcgggc ccgggatcca ccggatctag ataactgatc
     6481 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     6541 cccctgaacc tgaaacataa aatgaatgca attaaacccg ctgatcagcc tcgactgtgc
     6601 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     6661 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     6721 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     6781 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     6841 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     6901 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     6961 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     7021 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     7081 agggtgatgg ttcacgtacc tagaagttcc tattccgaag ttcctattct ctagaaagta
     7141 taggaacttc cttggccaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga
     7201 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg
     7261 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg
     7321 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg
     7381 aagtgcttga cattggggag ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac
     7441 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg
     7501 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat
     7561 tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg
     7621 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc
     7681 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc
     7741 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg
     7801 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga
     7861 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc
     7921 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc
     7981 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa
     8041 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg
     8101 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc
     8161 gtccgagggc aaaggaatag cacgtactac gagatttcga ttccaccgcc gccttctatg
     8221 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     8281 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     8341 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     8401 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     8461 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     8521 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     8581 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8641 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8701 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8761 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8821 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8881 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8941 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     9001 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     9061 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     9121 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     9181 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9241 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9301 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     9361 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9421 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9481 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9541 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9601 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9661 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9721 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9781 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9841 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9901 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9961 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    10021 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    10081 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    10141 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    10201 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    10261 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    10321 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    10381 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    10441 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    10501 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    10561 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10621 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
    10681 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
    10741 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
    10801 ttg
//
