LOCUS       sequence-272648-       10048 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid contains basal promoter driving firefly 
            Luciferase 2 and Renilla Luciferase driven by TK promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10048)
  AUTHORS   Sissaoui S, Yu J, Yan A, Li R, Zhu LJ, Kucukural A, Yukselen O, 
            Lawson ND
  TITLE     Genomic Characterization of Endothelial Enhancers Reveals a 
            Multifunctional Role for NR2F2 in Regulation of Arteriovenous Gene 
            Expression.
  JOURNAL   Circ Res. 2020 Feb 17. doi: 10.1161/CIRCRESAHA.119.316075.
  PUBMED    32065070
REFERENCE   2  (bases 1 to 10048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Circ Res. 
            2020 Feb 17. doi: 10.1161/CIRCRESAHA.119.316075."
COMMENT     Sequence Label: pLenti-basP:fLuc-TK:rLuc
FEATURES             Location/Qualifiers
     source          1..10048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1919
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1987..2007
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2009..2027
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2062..2086
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2093..2124
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2151..2160
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2157..3809
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     protein_bind    complement(3823..3847)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3848..4599
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     intron          4669..4801
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4784..4803
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        4846..4864
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4874..5809
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     protein_bind    complement(5857..5877)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    5892..6480
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5945..5965)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6363..6374)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6567..6800
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6872..6993
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6909..6928)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6963..6982
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7033..7168
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7189..7207)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7217..7234)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7217..7233)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7226..7248)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7375..7830
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7462..7481)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7672..7693
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7856..7960
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7961..8029,8030..8821)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8179..8198)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8992..9580
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9481..9500
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9734..9751
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9868..9889
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9904..9909,9910..9927,9928..9934)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9942..9958
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9947..9969
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9966..9982
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9966..9982
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10001..10021
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10003..10021
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta
     1921 tcgatcacga gactagcctc ctagcccggg ctcgagcagc tgaagcttgc atgcctgcag
     1981 ggctatcaac tttgtataga aaagttggta atacgactca ctatagggcg aattgggtac
     2041 ctcgagttaa cggatccccg ggcaagtttg tacaaaaaag caggctagac actagagggt
     2101 atataatgga agctcgactt ccagcttggc aatccggtac tgttggtaaa gccaccatgg
     2161 aagatgccaa aaacattaag aagggcccag cgccattcta cccactcgaa gacgggaccg
     2221 ccggcgagca gctgcacaaa gccatgaagc gctacgccct ggtgcccggc accatcgcct
     2281 ttaccgacgc acatatcgag gtggacatta cctacgccga gtacttcgag atgagcgttc
     2341 ggctggcaga agctatgaag cgctatgggc tgaatacaaa ccatcggatc gtggtgtgca
     2401 gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc cctgttcatc ggtgtggctg
     2461 tggccccagc taacgacatc tacaacgagc gcgagctgct gaacagcatg ggcatcagcc
     2521 agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa gatcctcaac gtgcaaaaga
     2581 agctaccgat catacaaaag atcatcatca tggatagcaa gaccgactac cagggcttcc
     2641 aaagcatgta caccttcgtg acttcccatt tgccacccgg cttcaacgag tacgacttcg
     2701 tgcccgagag cttcgaccgg gacaaaacca tcgccctgat catgaacagt agtggcagta
     2761 ccggattgcc caagggcgta gccctaccgc accgcaccgc ttgtgtccga ttcagtcatg
     2821 cccgcgaccc catcttcggc aaccagatca tccccgacac cgctatcctc agcgtggtgc
     2881 catttcacca cggcttcggc atgttcacca cgctgggcta cttgatctgc ggctttcggg
     2941 tcgtgctcat gtaccgcttc gaggaggagc tattcttgcg cagcttgcaa gactataaga
     3001 ttcaatctgc cctgctggtg cccacactat ttagcttctt cgctaagagc actctcatcg
     3061 acaagtacga cctaagcaac ttgcacgaga tcgccagcgg cggggcgccg ctcagcaagg
     3121 aggtaggtga ggccgtggcc aaacgcttcc acctaccagg catccgccag ggctacggcc
     3181 tgacagaaac aaccagcgcc attctgatca cccccgaagg ggacgacaag cctggcgcag
     3241 taggcaaggt ggtgcccttc ttcgaggcta aggtggtgga cttggacacc ggtaagacac
     3301 tgggtgtgaa ccagcgcggc gagctgtgcg tccgtggccc catgatcatg agcggctacg
     3361 ttaacaaccc cgaggctaca aacgctctca tcgacaagga cggctggctg cacagcggcg
     3421 acatcgccta ctgggacgag gacgagcact tcttcatcgt ggaccggctg aagagcctga
     3481 tcaaatacaa gggctaccag gtagccccag ccgaactgga gagcatcctg ctgcaacacc
     3541 ccaacatctt cgacgccggg gtcgccggcc tgcccgacga cgatgccggc gagctgcccg
     3601 ccgcagtcgt cgtgctggaa cacggtaaaa ccatgaccga gaaggagatc gtggactatg
     3661 tggccagcca ggttacaacc gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg
     3721 tgcctaaagg actgaccggc aagttggacg cccgcaagat ccgcgagatt ctcattaagg
     3781 ccaagaaggg cggcaagatc gccgtgtaat aattctagag tcacccagct ttcttgtaca
     3841 aagtggaaat gagtcttcgg acctcgcggg ggccgcttaa gcggtggtta gggtttgtct
     3901 gacgcggggg gagggggaag gaacgaaaca ctctcattcg gaggcggctc ggggtttggt
     3961 cttggtggcc acgggcacgc agaagagcgc cgcgatcctc ttaagcaccc ccccgccctc
     4021 cgtggaggcg ggggtttggt cggcgggtgg taactggcgg gccgctgact cgggcgggtc
     4081 gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac ccccgggcgg cgccgccgcg
     4141 gcggcgacgg gctcgctggg tcctaggctc catggggacc gtatacgtgg acaggctctg
     4201 gagcatccgc acgactgcgg tgatattacc ggagaccttc tgcgggacga gccgggtcac
     4261 gcggctgacg cggagcgtcc gttgggcgac aaacaccagg acggggcaca ggtacactat
     4321 cttgtcaccc ggaggcgcga gggactgcag gagcttcagg gagtggcgca gctgcttcat
     4381 ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt
     4441 ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac
     4501 gcagatgcag tcggggcggc gcggtcccag gtccacttcg catattaagg tgacgcgtgt
     4561 ggcctcgaac accgagcgac cctgcagcga cccgcttaaa agcttgattc ttctgacaca
     4621 acagtctcga acttaagctg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag
     4681 gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc
     4741 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca
     4801 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta
     4861 taggctagcc accatgactt cgaaagttta tgatccagaa caaaggaaac ggatgataac
     4921 tggtccgcag tggtgggcca gatgtaaaca aatgaatgtt cttgattcat ttattaatta
     4981 ttatgattca gaaaaacatg cagaaaatgc tgttattttt ttacatggta acgcggcctc
     5041 ttcttattta tggcgacatg ttgtgccaca tattgagcca gtagcgcggt gtattatacc
     5101 agaccttatt ggtatgggca aatcaggcaa atctggtaat ggttcttata ggttacttga
     5161 tcattacaaa tatcttactg catggtttga acttcttaat ttaccaaaga agatcatttt
     5221 tgtcggccat gattggggtg cttgtttggc atttcattat agctatgagc atcaagataa
     5281 gatcaaagca atagttcacg ctgaaagtgt agtagatgtg attgaatcat gggatgaatg
     5341 gcctgatatt gaagaagata ttgcgttgat caaatctgaa gaaggagaaa aaatggtttt
     5401 ggagaataac ttcttcgtgg aaaccatgtt gccatcaaaa atcatgagaa agttagaacc
     5461 agaagaattt gcagcatatc ttgaaccatt caaagagaaa ggtgaagttc gtcgtccaac
     5521 attatcatgg cctcgtgaaa tcccgttagt aaaaggtggt aaacctgacg ttgtacaaat
     5581 tgttaggaat tataatgctt atctacgtgc aagtgatgat ttaccaaaaa tgtttattga
     5641 atcggaccca ggattctttt ccaatgctat tgttgaaggt gccaagaagt ttcctaatac
     5701 tgaatttgtc aaagtaaaag gtcttcattt ttcgcaagaa gatgcacctg atgaaatggg
     5761 aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat gaacaataat tctagagcgg
     5821 ccgcttcgag cagacatgat aagatacatt gatgagcaac tttattatac atagttgata
     5881 attcactcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     5941 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6001 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6061 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6121 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6181 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6241 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     6301 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6361 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     6421 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     6481 ctggaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     6541 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     6601 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     6661 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6721 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6781 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     6841 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     6901 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6961 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     7021 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7081 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     7141 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     7201 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     7261 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     7321 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     7381 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     7441 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     7501 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     7561 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     7621 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     7681 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     7741 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     7801 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     7861 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     7921 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     7981 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     8041 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     8101 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     8161 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     8221 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     8281 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     8341 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     8401 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     8461 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     8521 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     8581 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     8641 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     8701 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     8761 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     8821 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     8881 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     8941 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     9001 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     9061 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     9121 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     9181 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     9241 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     9301 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     9361 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     9421 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     9481 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     9541 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     9601 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     9661 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     9721 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     9781 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     9841 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     9901 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     9961 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
    10021 ggaacaaaag ctggagctgc aagcttaa
//
