LOCUS       sequence-272656-        2940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and T174A
            mutant fused to the C-terminal half of split fluorescent Venus (VC).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2940)
  TITLE     Mammalian expression plasmids for production of metabotropic 
            glutamate receptor type 3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCEP4_HLA-cmyc-GRM3 C127A T174A-VC
FEATURES             Location/Qualifiers
     source          1..2940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             91..120
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2680..2931
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     2868..2889
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctagcgcca ccatggccgt catggcgccc cgaaccctcg tcctgctact ctcgggggcc
       61 ctggccctga cccagacctg ggcgggctcc gaacaaaagc tcatttctga agaggacttg
      121 ggaggatcca ggagagagat taaaatagaa ggtgaccttg ttttaggggg cctgtttcct
      181 attaacgaaa aaggcactgg aactgaagaa tgtgggcgaa tcaatgaaga ccgagggatt
      241 caacgcctgg aagccatgtt gtttgctatt gatgaaatca acaaagatga ttacttgcta
      301 ccaggagtga agttgggtgt tcacattttg gatacatgtt caagggatac ctatgcattg
      361 gagcaatcac tggagtttgt cagggcatct ttgacaaaag tggatgaagc tgagtatatg
      421 gctcctgatg gatcctatgc cattcaagaa aacatcccac ttctcattgc aggggtcatt
      481 ggtggctctt atagcagtgt ttccatacag gtggcaaacc tgctgcggct cttccagatc
      541 cctcagatca gctacgcatc cgccagcgcc aaactcagtg ataagtcgcg ctatgattac
      601 tttgccagga ccgtgccccc cgacttctac caggccaaag ccatggctga gatcttgcgc
      661 ttcttcaact ggacctacgt gtccacagta gcctccgagg gtgattacgg ggagacaggg
      721 atcgaggcct tcgagcagga agcccgcctg cgcaacatct gcatcgctac ggcggagaag
      781 gtgggccgct ccaacatccg caagtcctac gacagcgtga tccgagaact gttgcagaag
      841 cccaacgcgc gcgtcgtggt cctcttcatg cgcagcgacg actcgcggga gctcattgcg
      901 gccgccagcc gcgccaatgc ctccttcacc tgggtggcca gcgacggctg gggcgcgcag
      961 gagagcatca tcaagggcag cgagcatgtg gcctacggcg ccatcaccct ggagctggcc
     1021 tcccagcctg tccgccagtt cgaccgctac ttccagagcc tcaaccccta caacaaccac
     1081 cgcaacccct ggttccggga cttctgggag caaaagtttc agtgcagcct ccagaacaaa
     1141 cgcaaccaca ggcgcgtctg cgacaagcac ctggccatcg acagcagcaa ctacgagcaa
     1201 gagtccaaga tcatgtttgt ggtgaacgcg gtgtatgcca tggcccacgc tttgcacaaa
     1261 atgcagcgca ccctctgtcc caacactacc aagctttgtg atgctatgaa gatcctggat
     1321 gggaagaagt tgtacaagga ttacttgctg aaaatcaact tcacggctcc attcaaccca
     1381 aataaagatg cagatagcat agtcaagttt gacacttttg gagatggaat ggggcgatac
     1441 aacgtgttca atttccaaaa tgtaggtgga aagtattcct acttgaaagt tggtcactgg
     1501 gcagaaacct tatcgctaga tgtcaactct atccactggt cccggaactc agtccccact
     1561 tcccagtgca gcgacccctg tgcccccaat gaaatgaaga atatgcaacc aggggatgtc
     1621 tgctgctgga tttgcatccc ctgtgaaccg tacgaatacc tggctgatga gtttacctgt
     1681 atggattgtg ggtctggaca gtggcccact gcagacctaa ctggatgcta tgaccttcct
     1741 gaggactaca tcaggtggga agacgcctgg gccattggcc cagtcaccat tgcctgtctg
     1801 ggttttatgt gtacatgcat ggttgtaact gtttttatca agcacaacaa cacacccttg
     1861 gtcaaagcat cgggccgaga actctgctac atcttattgt ttggggttgg cctgtcatac
     1921 tgcatgacat tcttcttcat tgccaagcca tcaccagtca tctgtgcatt gcgccgactc
     1981 gggctgggga gttccttcgc tatctgttac tcagccctgc tgaccaagac aaactgcatt
     2041 gcccgcatct tcgatggggt caagaatggc gctcagaggc caaaattcat cagccccagt
     2101 tctcaggttt tcatctgcct gggtctgatc ctggtgcaaa ttgtgatggt gtctgtgtgg
     2161 ctcatcctgg aggccccagg caccaggagg tatacccttg cagagaagcg ggaaacagtc
     2221 atccttaagt gcaatgtcaa agattccagc atgttgatct ctcttaccta cgatgtgatc
     2281 ctggtgatct tatgcactgt gtacgccttc aaaacgcgga agtgcccaga aaatttcaac
     2341 gaagctaagt tcataggttt taccatgtac accacgtgca tcatctggtt ggccttcctc
     2401 cctatatttt atgtgacatc aagtgactac agagtgcaga cgacaaccat gtgcatctct
     2461 gtcagcctga gtggctttgt ggtcttgggc tgtttgtttg cacccaaggt tcacatcatc
     2521 ctgtttcaac cccagaagaa tgttgtcaca cacagactgc acctcaacag gttcagtgtc
     2581 agtggaactg ggaccacata ctctcagtcc tctgcaagca cgtatgtgcc aacggtgtgc
     2641 aatgggcggg aagtcctcga ctccaccacc tcatctctgg acaagcagaa gaacggcatc
     2701 aaggccaact tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac
     2761 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     2821 agctaccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     2881 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaactcgag
//
