LOCUS       sequence-272657-       10803 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of CENP-C and KNL2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10803)
  AUTHORS   McKinley KL, Sekulic N, Guo LY, Tsinman T, Black BE, Cheeseman IM
  TITLE     The CENP-L-N Complex Forms a Critical Node in an Integrated Meshwork
            of Interactions at the Centromere-Kinetochore Interface.
  JOURNAL   Mol Cell. 2015 Dec 17;60(6):886-98. doi: 
            10.1016/j.molcel.2015.10.027. Epub 2015 Nov 19.
  PUBMED    26698661
REFERENCE   2  (bases 1 to 10803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.10.027"; journalName: "Mol Cell"; date: 
            "2015-12-17- 17"; volume: "60"; issue: "6"; pages: "886-98"
COMMENT     Sequence Label: pKM294
FEATURES             Location/Qualifiers
     source          1..10803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        90..469
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        470..673
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     624..644
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    675..693
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    696..714
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     724..748
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      837..846
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             843..1550
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(988..1006)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1256..1275
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1596..1616
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1629..1673
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(6610..6627)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6616..6840
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6973..6992)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7124..7171
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7179..8201
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8331..8452
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8368..8387)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8422..8441
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8501..8517)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8501..8517)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8514..8536)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8525..8541
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8549..8555,8556..8573,8574..8579))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8594..8615
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8732..8749)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8903..9491)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8983..9002)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9662..10453,10454..10522))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10285..10304
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10523..10627)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10702..10721)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
       61 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
      121 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      181 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      241 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      301 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      361 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      421 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      481 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      541 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      601 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      661 tataagcaga gctctcccta tcagtgatag agatctccct atcagtgata gagatcgtcg
      721 acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
      781 ccatagaaga caccgggacc gatccagcct ccggactcta gcgtttgcta ccggtcgcca
      841 ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
      901 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
      961 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     1021 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     1081 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     1141 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     1201 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     1261 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     1321 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     1381 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     1441 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     1501 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag tccggagggg
     1561 atcggtggag ctccaccggt ggcggccgct ctagagagaa tctttatttt cagggcgccg
     1621 ccaaattcaa agaaaccgct gctgctaaat tcgaacgcca gcacatggac agcggaggtg
     1681 gaggtactag tctcgaggaa gaagtgactt caaccgttac caaaagccgc cgtattagtc
     1741 gtcgtcctag cgattggtgg gttgtgaaaa gcgaagaaag cccggtgtat agcaattcta
     1801 gcgtccgtaa tgagctgcct atgcaccata acagttcccg taaaagcacc aaaaaaacca
     1861 accagtctag caaaaacatt cgtaaaaaaa cgatccctct gaaacgccag aaaaccgcca
     1921 caaaaggcaa ccaacgtgtt cagaaattcc tgaacgccga aggatcagga ggcattgttg
     1981 ggcacgatga gattagtcgc tgctccctgt cagaacctct ggaatccgat gaagccgatc
     2041 tggctaaaaa aaaaaacctg gactgctccc gttctacccg tagcagtaaa aatgaggata
     2101 acatcatgac cgcccagaat gtcccactga aacctcagac aagcggttat acctgtaaca
     2161 ttccgaccga aagcaatctg gacagcggtg agcacaaaac aagtgtcctg gaagagtctg
     2221 gtcctagtcg tctgaataac aattatctga tgtccgggaa aaatgatgtg gacgatgagg
     2281 aagttcacgg tagtagcgac gattcaaaac agagtaaagt catccctaaa aatcgtattc
     2341 accataaact ggtgctgccg agtaatactc cgaacgttcg tcgtacaaaa cgtacacgcc
     2401 tgaaacctct ggaatattgg cgtggtgaac gtatcgacta ccagggtcgt ccatctggtg
     2461 gttttgtaat cagcggtgtg ctgagtcctg atacgattag cagcaaacgt aaagccaaag
     2521 agaacatcgg caaagtcaac aaaaaatcca acaaaaaacg catctgcctg gataacgatg
     2581 agcgtaaaac gaatctgatg gtgaatctgg gtattcctct gggagatcct ctgcagccta
     2641 cccgtgtaaa agatcctgag acacgtgaga ttatcctgat ggacctggtt cgtcctcaag
     2701 acacctatca gtttttcgtc aaacacggcg agctgaaagt ctataaaacc ctggacaccc
     2761 cgttctttag taccggcaaa ctgattctgg gtcctcagga ggaaaaaggc aaacagcacg
     2821 tgggtcaaga catcctggtc ttttatgtga attttgggga cctgctgtgt actctgcatg
     2881 aaaccccgta tattctgtct accggtgaca gcttctatgt gccgtctgga aactattata
     2941 acatcaaaaa cctgcgcaac gaagaaagtg ttctgctgtt cacacagatc aaacgcggtt
     3001 ctggctccgg atcagggtcg ggtagtggca gcggttcggg ctcgatgatt gcaacacctt
     3061 tgaaacattc aagaatttac ttacctccag aggcatcttc tcaaaggaga aatctaccca
     3121 tggatgcaat cttttttgac agcattcctt caggcacact tactcctgta aaagatttgg
     3181 tgaaatatca gaactcctcc ttaaaattga atgaccataa aaagaatcag ttcctaaaaa
     3241 tgacaacttt taacaataaa aatatatttc aatcaactat gctaacagag gctactacct
     3301 ctaacagttc tcttgatatc agtgctataa agcccaacaa ggatggatta aaaaataaag
     3361 caaactatga atcaccagga aaaatatttc taagaatgaa agaaaaagta ctgcgtgaca
     3421 agcaagaaca gccatcaaga aacagtagtt tgttggaacc acagaaaagt ggaaataatg
     3481 aaaccttcac tcctaacaga gttgaaaaaa aaaaattgca gcatacctac ctatgtgaag
     3541 aaaaggaaaa caacaaatca ttccagtcag atgacagttc actaagagcc tcagtccaag
     3601 gagttcctct agaatcatca aataatgata ttttcctccc ggtcaaacaa aagattcagt
     3661 gccagcagga aaagaaagca ccactgcaca atttaactta cgaacttcca actctgaacc
     3721 aagaacagga aaattttttg gctgtagaag cccgaaacaa gacattaact agagctcagt
     3781 tggctaaaca aatttttcac tcaaaggaga gtatagttgc aaccactaaa tccaaaaagg
     3841 acacgtttgt tttagaaagc gttgattctg ctgatgaaca atttcaaaat actaatgctg
     3901 agactctcag tactaattgt attcctatta aaaatggcag cctgttaatg gtttctgata
     3961 gtgagaggac aacagaaggg acttcgcaac agaaagttaa ggaaggaaat ggaaaaacag
     4021 tgcctggaga gacaggtctt ccaggttcca tgaaagatac atgtaaaatt gtacttgcaa
     4081 caccaagact tcatataaca atacctcgga ggtcaaaaag aaatatttca aagctttctc
     4141 ctccaagaat atttcaaact gttacaaatg gacttaaaaa aaatcaggta gttcagctac
     4201 aggaatggat gattaaaagc atcaataata atactgctat atgtgtagaa ggaaaattga
     4261 tagacgtcac taacatatat tggcacagta atgtaattat agagcggatt gagcacaaca
     4321 aacttaggac tatatcaggc aacgtttata tattaaaagg catgatagac caaatttcca
     4381 tgaaagaagc aggatatcca aattatctca taaggaaatt tatgtttgga tttccagaaa
     4441 attggaaaga gcacattgat aattttctgg aacaattaag ggctggtgaa aagaacaggg
     4501 aaaagaccaa acaaaaacag aaaactggaa gatctgtccg tgacataagg aaatcaatga
     4561 aaaatgatgc acgagaaaac caaacagata ctgctcaaag agccaccacc acttacgatt
     4621 ttgattgtga taatttggaa ctgaagagta ataagcacag tgagtcacca ggagctacag
     4681 aattaaacat gtgccacagt aattgccaaa ataaaccaac attaaggttc ccagatgacc
     4741 aagtaaataa tactattcaa aatggaggag gagatgactt atctaatcag gaattaattg
     4801 gaaaaaaaga atataaaatg tcttcaaaga aactaaaaat tggtgaaaga acaaatgaaa
     4861 ggataataaa aagtcagaag caagagacaa ctgaagaatt ggatgtatcc attgatattc
     4921 taacctcaag ggaacagttt ttctcagatg aagaaagaaa atacatggcc atcaatcaga
     4981 agaaagctta tattttagta acaccactta aatctagaaa agtgatagag caaagatgca
     5041 tgaggtataa tctgtccgct ggcaccatca aagcagtaac agattttgta ataccagagt
     5101 gtcaaaaaaa aagtcccatc agcaagtcca tggggacttt agaaaataca tttgaaggtc
     5161 ataaaagtaa aaacaaggaa gattgcgatg aacgtgactt acttactgtc aaccggaaaa
     5221 taaaaatatc taaccttgaa aaggaacaaa tgctcacctc tgactttaag aaaaatacca
     5281 gactattacc aaaattgaag aaaatagaaa atcaggtagc tatgtcattt tataagcatc
     5341 agtcctcacc agatttgtca agtgaagaaa gtgaaacaga aaaggaaatt aaaaggaaag
     5401 ctgaagttaa gaaaaccaaa gcaggaaaca ccaaagaagc agtggttcac ctgagaaaga
     5461 gcacaagaaa cacaagtaat attccagtga ttttggaacc tgaaactgaa gaaagtgaaa
     5521 atgaatttta tatcaaacaa aagaaagcta gaccttccgt caaagaaact cttcaaaaaa
     5581 gcggcgtgag aaaggagttt ccaattactg aggcagtagg atctgataag acaaataggc
     5641 atcccttaga atgcttacct ggtttaattc aggataagga atggaatgag aaggagttac
     5701 agaaacttca ttgtgctttt gcatctcttc caaagcacaa acctggtttc tggtcagagg
     5761 tagctgcggc tgtaggttct cgatctcctg aagaatgcca gaggaaatac atggaaaatc
     5821 ccagaggaaa aggatcccag aaacatgtca ctaagaagaa gccagccaat tccaaaggcc
     5881 aaaatggcaa gagaggtgat gctgatcaga aacaaactat taagataact gccaaagtgg
     5941 gaactcttaa aaggaagcaa cagatgaggg aatttctgga acagttgcca aaagatgacc
     6001 atgatgattt tttcagtaca acacctttac agcatcaaag aatactgttg ccaagtttcc
     6061 aggacagtga agatgatgat gatattctgc caaatatgga caaaaatcca acaactccat
     6121 catcagttat ctttccattg gtaaaaactc ctcaatgtca gcatgtcagt cctggcatgc
     6181 taggttctat aaataggaat gactgtgata aatatgtttt tcgtatgcaa aaatatcata
     6241 aaagtaatgg tggtattgtc tggggcaaca tcaagaaaaa attagttgaa actgatttct
     6301 caactccaac accaagaagg aaaaccccat ttaacacaga cttaggagaa aactctggta
     6361 ttggaaaact tttcactaat gctgtggaat ctttagatga agaagagaaa gattattatt
     6421 tttcgaactc tgattctgca taggaattct gcagtcgacg gtaccgcggg cccgggatcc
     6481 accggatcta gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct
     6541 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattaaaccc
     6601 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     6661 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     6721 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     6781 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     6841 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     6901 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     6961 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     7021 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     7081 tcgaccccaa aaaacttgat tagggtgatg gttcacgtac ctagaagttc ctattccgaa
     7141 gttcctattc tctagaaagt ataggaactt ccttggccaa aaagcctgaa ctcaccgcga
     7201 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct
     7261 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
     7321 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
     7381 cggccgcgct cccgattccg gaagtgcttg acattgggga gttcagcgag agcctgacct
     7441 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc
     7501 ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc
     7561 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg
     7621 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca
     7681 ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc
     7741 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg
     7801 gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg
     7861 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact
     7921 tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca
     7981 ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg
     8041 cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa
     8101 tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg
     8161 gaaaccgacg ccccagcact cgtccgaggg caaaggaata gcacgtacta cgagatttcg
     8221 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     8281 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     8341 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     8401 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     8461 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     8521 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     8581 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     8641 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     8701 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     8761 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     8821 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     8881 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     8941 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     9001 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     9061 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     9121 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     9181 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     9241 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     9301 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     9361 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     9421 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9481 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     9541 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9601 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     9661 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     9721 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     9781 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     9841 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     9901 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     9961 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    10021 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    10081 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    10141 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    10201 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    10261 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    10321 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    10381 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    10441 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    10501 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    10561 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    10621 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
    10681 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
    10741 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
    10801 aag
//
