LOCUS       sequence-27266-e         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Furukawa M, Xiong Y
  TITLE     BTB protein Keap1 targets antioxidant transcription factor Nrf2 for 
            ubiquitination by the Cullin 3-Roc1 ligase.
  JOURNAL   Mol Cell Biol. 2005 Jan . 25(1):162-71.
  PUBMED    15601839
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2005 Jan . 25(1):162-71."
COMMENT     Sequence Label: pcDNA3-HA2-Keap1 delta BTB
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             86..112
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             116..142
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             122..148
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 gcagagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctatgtaccc atacgatgtt cctgactatg cgggctatcc
      121 ctatgacgtc ccggactatg caagcttggt accgttaaca tggatcctgg accccagcaa
      181 tgccatcggc atcgccaact tcgctgagca gattggctgt gtggagttgc accagcgtgc
      241 ccgggagtac atctacatgc attttgggga ggtggccaag caagaggagt tcttcaacct
      301 gtcccactgc caactggtga ccctcatcag ccgggacgac ctgaacgtgc gctgcgagtc
      361 cgaggtcttc cacgcctgca tcaactgggt caagtacgac tgcgaacagc gacggttcta
      421 cgtccaggcg ctgctgcggg ccgtgcgctg ccactcgttg acgccgaact tcctgcagat
      481 gcagctgcag aagtgcgaga tcctgcagtc cgactcccgc tgcaaggact acctggtcaa
      541 gatcttcgag gagctcaccc tgcacaagcc cacgcaggtg atgccctgcc gggcgcccaa
      601 ggtgggccgc ctgatctaca ccgcgggcgg ctacttccga cagtcgctca gctacctgga
      661 ggcttacaac cccagtgacg gcacctggct ccggttggcg gacctgca
//
