LOCUS       sequence-272661-        8961 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral sgRNA plasmid with blasticidin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   McKinley KL, Cheeseman IM
  TITLE     Large-Scale Analysis of CRISPR/Cas9 Cell-Cycle Knockouts Reveals the
            Diversity of p53-Dependent Responses to Cell-Cycle Defects.
  JOURNAL   Dev Cell. 2017 Feb 27;40(4):405-420.e2. doi: 
            10.1016/j.devcel.2017.01.012. Epub 2017 Feb 16.
  PUBMED    28216383
REFERENCE   2  (bases 1 to 8961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.01.012"; journalName: "Dev Cell"; date: 
            "2017-02-27- 27"; volume: "40"; issue: "4"; pages: "405-420.e2"
COMMENT     Sequence Label: pKM808
FEATURES             Location/Qualifiers
     source          1..8961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    168..401
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             586..630
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        808..1056
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     808..828
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     979..998
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    3072..3189
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3258..3469
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      3489..3498
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3495..3893
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3909..4497
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3962..3982)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4380..4391)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4569..4802
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4880..5001
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4917..4936)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4971..4990
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5041..5176
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5196..5215)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5197..5215)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5225..5242)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5225..5241)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5234..5256)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5383..5838
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5470..5489)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5680..5701
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5864..5968
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5969..6037,6038..6829)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6187..6206)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7000..7588
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7489..7508
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7742..7759
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7876..7897
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7912..7917,7918..7935,7936..7942)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7950..7966
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7955..7977
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7974..7990
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7974..7990
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8009..8029
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8011..8029
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8057..8283
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8284..8464
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8511..8636
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
       61 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
      121 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
      181 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
      241 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
      301 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
      361 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
      421 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
      481 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
      541 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
      601 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
      661 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
      721 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
      781 cacctcccaa ccccgagggg acccagagag ggcctatttc ccatgattcc ttcatatttg
      841 catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag
      901 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
      961 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     1021 ttggctttat atatcttgtg gaaaggacga aacaccggag acggttgtaa atgagcacac
     1081 aaaatacaca tgctaaaata ttatattcta tgacctttat aaaatcaacc aaaatcttct
     1141 ttttaataac tttagtatca ataattagaa tttttatgtt cctttttgca aacttttaat
     1201 aaaaatgagc aaaataaaaa aacgctagtt ttagtaactc gcgttgtttt cttcaccttt
     1261 aataatagct actccaccac ttgttcctaa gcggtcagct cctgcttcaa tcattttttg
     1321 agcatcttca aatgttctaa ctccaccagc tgctttaact aaagcattgt ctttaacaac
     1381 tgacttcatt agtttaacat cttcaaatgt tgcacctgat tttgaaaatc ctgttgatgt
     1441 tttaacaaat tctaatccag cttcaacagc tatttcacaa gctttcatga tttcttcttt
     1501 tgttaataaa caattttcca taatacattt aacaacatgt gatccagctg ctttttttac
     1561 agctttcatg tcttctaaaa ctaattcata atttttgtct tttaatgcac caatatttaa
     1621 taccatatca atttctgttg caccatcttt aattgcttca gaaacttcga atgcttttgt
     1681 agctgttgtg catgcaccta gaggaaaacc tacaacattt gttattccta catttgtgcc
     1741 ttttaataat tctttacaat agcttgttca atatgaatta acacaaactg ttgcaaaatc
     1801 aaattcaatt gcttcatcac ataattgttt aatttcagct ttcgtagcat cttgttttaa
     1861 taatgtgtga tctatatatt tgtttagttt cattttttct cctatatatt catttttaat
     1921 tttaattctt taataatttc gtctacttta actttagcgt tttgaacaga ttcaccaaca
     1981 cctataaaat aaatttttag tttaggttca gttccacttg ggcgaacagc aaatcatgac
     2041 ttatcttcta aataaaattt tagtaagtct tgtcctggca tattatacat tccatcgatg
     2101 tagtcttcaa cattaacaac tttaagtcca gcaatttgag ttaagggtgt tgctctcaat
     2161 gatttcatta atggttcaat ttttaatttc ttttcttctg gtttaaaatt caagtttaaa
     2221 gtgaaagtgt aatatgcacc catttcttta aataaatctt ctaaatagtc tactaatgtt
     2281 ttattttgtt ttttataaaa tcaagcagcc tctgctatta atatagaagc ttgtattcca
     2341 tctttatctc tagctgagtc atcaattaca tatccataac tttcttcata agcaaaaaca
     2401 aaatttaatc cgttatcttc ttctttagca atttctctac ccattcattt aaatccagtt
     2461 aaagttttta caatattaac tccatatttt tcatgagcga ttctatcacc caaatcactt
     2521 gttacaaaac ttgaatatag agccggattt tttggaatgc tatttaagcg ttttagattt
     2581 gataattttc aatcaattaa aattggtcct gtttgatttc catctaatct tacaaaatga
     2641 ccatcatgtt ttattgccat tccaaatctg tcagcatctg ggtcattcat aataataata
     2701 tctgcatcat gtttaatacc atattcaagc ggtatttttc atgcaggatc aaattctgga
     2761 tttggattta caacattttt aaatgtttca tcttcaaatg catgctcttc aacctcaata
     2821 acgttatatc ctgattcacg taatattttt ggggtaaatt tagttcctgt tccattaact
     2881 gcgctaaaaa taatttttaa atctttttta gcttcttgct cttttttgta cgtctctgtt
     2941 taagagctat gctggaaaca gcatagcaag tttaaataag gctagtccgt tatcaacttg
     3001 aaaaagtggc accgagtcgg tgcttttttt gaattctaga tcttgagaca aatggcagta
     3061 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     3121 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     3181 caaaattttc gggtttatta cagggacagc agagatccac tttggcgccg gctcgagtgg
     3241 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     3301 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     3361 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     3421 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg tgtcgtgacg
     3481 cgggatccgc caccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag
     3541 caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc
     3601 tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt
     3661 gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta
     3721 tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg
     3781 tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga
     3841 cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taaacgcgtt
     3901 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     3961 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     4021 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     4081 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     4141 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     4201 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     4261 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     4321 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     4381 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     4441 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc
     4501 gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     4561 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     4621 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     4681 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     4741 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     4801 cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     4861 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     4921 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     4981 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     5041 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     5101 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     5161 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     5221 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     5281 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     5341 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     5401 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     5461 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     5521 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     5581 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     5641 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     5701 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     5761 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     5821 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     5881 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     5941 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     6001 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     6061 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     6121 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     6181 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     6241 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     6301 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     6361 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     6421 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     6481 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     6541 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     6601 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     6661 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     6721 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     6781 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     6841 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     6901 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     6961 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7021 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7081 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7141 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7201 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7261 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7321 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7381 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7441 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7501 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7561 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     7621 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     7681 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     7741 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     7801 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     7861 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     7921 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     7981 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     8041 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
     8101 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
     8161 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga
     8221 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata
     8281 aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     8341 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     8401 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     8461 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     8521 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     8581 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     8641 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     8701 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     8761 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     8821 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     8881 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     8941 agcaaaacaa aagtaagacc a
//
