LOCUS       sequence-27267-e         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Furukawa M, Xiong Y
  TITLE     BTB protein Keap1 targets antioxidant transcription factor Nrf2 for 
            ubiquitination by the Cullin 3-Roc1 ligase.
  JOURNAL   Mol Cell Biol. 2005 Jan . 25(1):162-71.
  PUBMED    15601839
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2005 Jan . 25(1):162-71."
COMMENT     Sequence Label: pcDNA3-HA2-Keap1 S3H4
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             86..112
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             116..142
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             122..148
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 gcagagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctatgtaccc atacgatgtt cctgactatg cgggctatcc
      121 ctatgacgtc ccggactatg caagcttggt accgttaaca tggatcccca tgcagccaga
      181 tcccaggcct agcggggctg gggcctgctg ccgattcctg cccctgcagt cacagtgccc
      241 tgagggggca ggggacgcgg tgatgtacgc ctccactgag tgcaaggcgg aggtgacgcc
      301 ctcccagcat ggcaaccgca ccttcagcta caccctggag gatcatacca agcaggcctt
      361 tggcatcatg aacgagctgc ggctcagcca gcagctgtgt gacgtcacac tgcaggtcaa
      421 gtaccaggat gcaccggccg cccagttcat ggcccacaag gtggtgctgg cctcatccag
      481 ccctgtcttc aaggccatgt tcaccaacgg gctgcgggag cagggcatgg aggtggctag
      541 cgctgaggct atccacccca aggtcatgga gcgcctcatt gaattcgcct acacggcctc
      601 catctccatg ggcgagaagt gtgtcctcca cgtcatgaac ggtgccgcgg cgtaccagat
      661 cgacagcgtt gtccgtgcct gcagtgactt cctggtgcag caactggacc ccagcatgcc
      721 atcggcatcg ccacttcgct
//
