LOCUS       sequence-27270-e         595 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Liu B, Paton JF, Kasparov S
  TITLE     Viral vectors based on bidirectional cell-specific mammalian 
            promoters and transcriptional amplification strategy for use in 
            vitro and in vivo.
  JOURNAL   BMC Biotechnol. 2008 May 16;8:49. doi: 10.1186/1472-6750-8-49.
  PUBMED    18485188
REFERENCE   2  (bases 1 to 595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2008-05-16"; volume: "8:49. doi"; pages: " 
            10.1186/1472-6750-8-49"
COMMENT     Sequence Label: pTYF-mCMV/SYN-EGFP
FEATURES             Location/Qualifiers
     source          1..595
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(79..526)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcac tagtggatcc gagctcggta ccaagcttgc
       61 ctgcagctct agagtcgact gcgctctcag gcacgacacg actcctccgc tgcccaccgc
      121 agactgaggc agcgctgagt cgccggcgcc gcagcgcaga tggtcgcgcc cgtgcccccc
      181 tatctcgcgc ctcgcgtggt gcggtccggc tgggccggcg gcggcgcgga cgcgaccaag
      241 gtggccggga aggggagttt gcgggggacc ggcgagtgac gtcagcgcgc cttcagtgct
      301 gaggcggcgg tggcgcgcgc cgccaggcgg gggcgaaggc actgtccgcg gtgctgaagc
      361 tggcagtgcg cacgcgcctc gccgcatcct gtttcccctc cccctctctg ataggggatg
      421 cgcaatttgg ggaatggggg ttgggtgctt gtccagtggg tcggggtcgg tcgtcaggta
      481 ggcaccccca ccccgcctca tcctggtcct aaaacccact tgcactcata cgcagggccc
      541 tctgcagctg gcgccatcga tacgcgtccc tctagagtct ccgctcggag gacag
//
