LOCUS       sequence-272707-       10002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  OsTIR1(WT)-P2A-mAID-EGFP-NLS TOL2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10002)
  AUTHORS   Yesbolatova A, Saito Y, Kitamoto N, Makino-Itou H, Ajima R, Nakano 
            R, Nakaoka H, Fukui K, Gamo K, Tominari Y, Takeuchi H, Saga Y, 
            Hayashi KI, Kanemaki MT
  TITLE     The auxin-inducible degron 2 technology provides sharp degradation 
            control in yeast, mammalian cells, and mice.
  JOURNAL   Nat Commun. 2020 Nov 11;11(1):5701. doi: 10.1038/s41467-020-19532-z.
  PUBMED    33177522
REFERENCE   2  (bases 1 to 10002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-11-11"; volume: "11(1):5701. doi"; pages: " 
            10.1038/s41467-020-19532-z"
COMMENT     Sequence Label: pAY7 (OsTIR1 mAID-EGFP-NLS TOL2)
FEATURES             Location/Qualifiers
     source          1..10002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(679..690,691..853)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    complement(884..917)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(1474..2073)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     2054..2073
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(2113..2612)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2161..2180)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     2554..2577
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    complement(2673..2706)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    2733..2854
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2744..2763)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2798..2817
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3005..3025)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(3062..3772,3773..3775,3776..3778))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(3062..3772,3773..3775,3776..3778))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3104..3125)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3453..3472
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3712..3733
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(3785..3988)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             complement(3995..4051)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(4067..5791)
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     primer_bind     complement(5836..5855)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          5863..6880
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(5917..5939)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(6882..7159)
                     /label=chicken beta-actin promoter
     enhancer        7161..7540
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    join(7562..7749,7750..7761)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7818..7838)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7818..7836)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7857..7873)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7857..7873)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7870..7892)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7881..7897
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7905..7911,7912..7929,7930..7935))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7950..7971
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8088..8105)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8259..8847)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8339..8358)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9018..9809,9810..9878))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9641..9660
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9879..9983)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 ccggcatatg gttcttgaca gaggtgtaaa aagtactcaa aaattttact caagtgaaag
      721 tacaagtact tagggaaaat tttactcaat taaaagtaaa agtatctggc tagaatctta
      781 cttgagtaaa agtaaaaaag tactccatta aaattgtact tgagtattaa ggaagtaaaa
      841 gtaaaagcaa gaaagatctt agtgaacctc ttcgagggac ctaataactt cgtatagcat
      901 acattatacg aagttatatt aagggttccg gatctcgacc agcttctgat ggaattagaa
      961 cttggcaaaa caatactgag aatgaagtgt atgtggaaca gaggctgctg atctcgttct
     1021 tcaggctatg aaactgacac atttggaaac cacagtactt agaaccacaa agtgggaatc
     1081 aagagaaaaa caatgatccc acgagagatc tatagatcta tagatcatga gtgggaggaa
     1141 tgagctggcc cttaatttgg ttttgcttgt ttaaattatg atatccaact atgaaacatt
     1201 atcataaagc aatagtaaag agccttcagt aaagagcagg catttatcta atcccacccc
     1261 acccccaccc ccgtagctcc aatccttcca ttcaaaatgt aggtactctg ttctcaccct
     1321 tcttaacaaa gtatgacagg aaaaacttcc attttagtgg acatctttat tgtttaatag
     1381 atcatcaatt tctgcatccc ggggatctga tatcatcgat gcatggggtc gtgcgctcct
     1441 ttcggtcggg cgctgcgggt cgtggggcgg gcgtcaggca ccgggcttgc gggtcatgca
     1501 ccaggtgcgc ggtccttcgg gcacctcgac gtcggcggtg acggtgaagc cgagccgctc
     1561 gtagaagggg aggttgtggg gcgcggaggt ctccaggaag gcgggcaccc cggcgcgctc
     1621 ggccgcctcc actccgggga gcacgacggc gctgcccaga cccttgccct ggtggtcggg
     1681 cgagacgccg acggtggcca ggaaccacgc gggctccttg ggccggtgcg gcgccaggag
     1741 gccttccatc tgttgctgcg cggccagccg ggaaccgctc aactcggcca tgcgcgggcc
     1801 gatctcggcg aacaccgccc ccgcttcgac gctctccggc gtggtccaga ccgccaccgc
     1861 ggcgccgtcg tccgcgaccc acaccttgcc gatgtcgagc ccgacgcgcg tgaggaagag
     1921 ttcttgcagc tcggtgaccc gctcgatgtg gcggtccgga tcgacggtgt ggcgcgtggc
     1981 ggggtagtcg gcgaacgcgg cggcgagggt gcgtacggcc ctggggacgt cgtcgcgggt
     2041 ggcgaggcgc accgtgggct tgtactcggt catggtaagc ttcagctgct cgagatctag
     2101 atggatgcag gtcgaaaggc ccggagatga ggaagaggag aacagcgcgg cagacgtgcg
     2161 cttttgaagc gtgcagaatg ccgggcctcc ggaggacctt cgggcgcccg ccccgcccct
     2221 gagcccgccc ctgagcccgc ccccggaccc accccttccc agcctctgag cccagaaagc
     2281 gaaggagcaa agctgctatt ggccgctgcc ccaaaggcct acccgcttcc attgctcagc
     2341 ggtgctgtcc atctgcacga gactagtgag acgtgctact tccatttgtc acgtcctgca
     2401 cgacgcgagc tgcggggcgg gggggaactt cctgactagg ggaggagtag aaggtggcgc
     2461 gaaggggcca ccaaagaacg gagtcggttg gcgcctaccg gtggatgtgg aatgtgtgcg
     2521 aggccagagg ccacttgtgt agcgccaagt gcccagcggg gctgctaaag cgcatgctcc
     2581 agactgcctt gggaaaagcg cctcccctac ccggtagaat ttcgaggtcg agatcctaag
     2641 cttggctgga cgtaaactcc tcttcagacc taataacttc gtatagcata cattatacga
     2701 agttatatta agggttattg aatatgatcg cttaagatac attgatgagt ttggacaaac
     2761 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     2821 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     2881 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     2941 tggtatggct gattatgatc agttatctag atccggtgga tcccgggccc gcggtaccgt
     3001 cgatgacctt tcttttcttc ttggggtcga ctgcagaatt cgaagcttgg gcgcctgcac
     3061 ccttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga
     3121 ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt
     3181 ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg
     3241 cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt
     3301 tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt
     3361 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
     3421 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga
     3481 agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct
     3541 tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg
     3601 tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt
     3661 aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt
     3721 ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgc
     3781 tagctttata catcctcaaa tcgattttcc tcaagtacgg tgctccgtcc attgatacct
     3841 tcacgaacgc cgccgcctcc gggccaccgc ttgatttttg gcaggaaacc atcacgttct
     3901 tccggtatga tctcaccggt ggccatccca caacttgtgc cttggccgga ggtttggctg
     3961 gatctttagg acaagcactc ttctccttag atctaggtcc agggttctcc tccacgtctc
     4021 cagcctgctt cagcaggctg aagttagtag ctccgcttcc tgatctcaga atcttcacaa
     4081 agttgggagc atcatctctt gcccctgcgg tggtccgata cacatacagt ttctccacct
     4141 ttggcaggtc gccatgattt tcctccattt cgttagaccc gtcccgctca ttaatgactt
     4201 ccacgttcag cataggcatt ttactagcca ggacctgaca gcccttcaga gtcacattgc
     4261 aactagacat ccacaggctc ctcattgtct cgtatctggc aaagtttccc agcagagcgg
     4321 catccccgaa agggctgtcc cggatttcca gttttcgcag attcttacac ccgttcatca
     4381 cgtgcatcat gcccttatcg gagtctccgg caaatgcaat gctcagcatc tccagctgct
     4441 tagcatactt gccgatgtac atgaacactt tgtctgtcag caggccggaa atgctcagcc
     4501 tctggagtcc cttgcactct ctcacgatag ctccaaaccc ttcatccagt ggctgggatg
     4561 tgaccacgtc gggtttgcct ggttccagaa tacacagccg aaatcgggtg aaattagggc
     4621 agttcttggc gacggtcacc agtgcagcat tagtcatctg atgacagaaa tacagcaggc
     4681 tgttcagctt tgggcatccc agtgagactg ccaccaggcc ttcctcagtg acagcactat
     4741 atccggccac gtaaaaatca gatgggaaca ctctcagctc ctggaggtcc ttgcaggagc
     4801 tggcgaccac ctggagcccc ttatcggaaa tacagtccag cacccacagt cgctggagtt
     4861 tcacgcagcg tgagatcatc tttgtcagat cactggcgtc cagggttggt gcatagctca
     4921 ggttcaggcc tgtcagctgt gcacacagag ggtagataaa gctcaggcac acgggtgaag
     4981 catcccagaa tcctgacaga ctcctcagca ttttacactt ctccagggca gaagtcagtt
     5041 taaagtagga ttctgtctgg aaatcgtctg tcagattgcc ggttcccaga tcctccaggt
     5101 taggggttct cagcagaatc ttagccagag tgtccacgct cacgctccgg ttcagccgca
     5161 gagacctcag gttggggctt cttgagacca gccgctccag ggacccagca ttcacttcgc
     5221 ctttgatgca ggcaaagttc agggacacca ggctggtaca tgagtctggg aagcaagaca
     5281 gccatctggg gcctctatcc tccacttcat tctcctggag gtccagctcc cgcagcagct
     5341 tacagtggga ggccacggct gccagtccat cggtgctgaa gccttcgcaa gaaatcagca
     5401 ccagtgcgcg aaacctaggg aagctccgag ccagcagctc cagactttcg tcagagacca
     5461 ccattcgctt catgcgcagt tcctccagtc catgacagcc gcgagcggct gcctcgatcc
     5521 aagggccggc gtatcctccc caatcagggg gcaccaggtt aaagtcggcg aagtggggtt
     5581 tccccttgac ggtcagtgcg cgcacatttg gaaacctagc ggcgaccctg cctgctctca
     5641 cagcatagca gttgccgacg aacactcctc tccggctcag gcgttcgatc tcgtaccaca
     5701 ctttgcagac caggctcact gtgtttctat ctctctgtgc aggcaggaag ctaaaaatgt
     5761 gttcgacgac ctcttcagga aagtatgtca tggtggcagc gcttcgagga attctttgcc
     5821 aaaatgatga gacagcacaa taaccagcac gttgcccagg agctgtagga aaaagaagaa
     5881 ggcatgaaca tggttagcag aggctctaga gccgccggtc acacgccaga agccgaaccc
     5941 cgccctgccc cgtccccccc gaaggcagcc gtccccccgc ggacagcccc gaggctggag
     6001 agggagaagg ggacggcggc gcggcgacgc acgaaggccc tccccgccca tttccttcct
     6061 gccggcgccg caccgcttcg ccccgcgccc gctagagggg gtgcggcggc gcctcccaga
     6121 tttcggctcc gcacagattt gggacaaagg aagtccctgc gccctctcgc acgattacca
     6181 taaaaggcaa tggctgcggc tcgccgcgcc tcgacagccg ccggcgctcc gggggccgcc
     6241 gcgcccctcc cccgagccct ccccggcccg aggcggcccc gccccgcccg gcacccccac
     6301 ctgccgccac cccccgcccg gcacggcgag ccccgcgcca cgccccgtac ggagccccgc
     6361 acccgaagcc gggccgtgct cagcaactcg gggagggggg tgcagggggg gttgcagccc
     6421 gaccgacgcg cccacacccc ctgctcaccc ccccacgcac acaccccgca cgcagccttt
     6481 gttcccctcg cagccccccc cgcaccgcgg ggcaccgccc ccggccgcgc tcccctcgcg
     6541 cacactgcgg agcgcacaaa gccccgcgcc gcgcccgcag cgctcacagc cgccgggcag
     6601 cgcggagccg cacgcggcgc tccccacgca cacacacacg cacgcacccc ccgagccgct
     6661 ccccccgcac aaagggccct cccggagccc ctcaaggctt tcacgcagcc acagaaaaga
     6721 aacaagccgt cattaaacca agcgctaatt acagcccgga ggagaagggc cgtcccgccc
     6781 gctcacctgt gggagtaacg cggtcagtca gagccggggc gggcggcgcg aggcggcggc
     6841 ggagcggggc acggggcgaa ggcagcgtcg cagcgactcc ccgcccgccg cgcgcttcgc
     6901 tttttatagg gccgccgccg ccgccgcctc gccataaaag gaaactttcg gagcgcgccg
     6961 ctctgattgg ctgccgccgc acctctccgc ctcgccccgc cccgcccctc gccccgcccc
     7021 gccccgcctg gcgcgcgccc cccccccccc cccgccccca tcgctgcaca aaataattaa
     7081 aaaataaata aatacaaaat tgggggtggg gagggggggg agatggggag agtgaagcag
     7141 aacgtggggc tcacctcgac catggtaata gcgatgacta atacgtagat gtactgccaa
     7201 gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca
     7261 ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca
     7321 gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg
     7381 ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg
     7441 ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc
     7501 gtaattgatt actattaata actagtcaat aatcaatgtc gagcatgcat ctagagggcc
     7561 caagtgatct ccaaaaaata agtacttttt gactgtaaat aaaattgtaa ggagtaaaaa
     7621 gtactttttt ttctaaaaaa atgtaattaa gtaaaagtaa aagtattgat ttttaattgt
     7681 actcaagtaa agtaaaaatc cccaaaaata atacttaagt acagtaatca agtaaaatta
     7741 ctcaagtact ttacacctct ggttcttgac cccctacctt cagcaagccc agcagatccg
     7801 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
     7861 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     7921 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     7981 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8041 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     8101 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8161 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8221 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8281 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8341 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8401 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     8461 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8521 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8581 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8641 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     8701 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8761 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8821 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8881 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8941 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9001 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9061 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9121 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9181 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9241 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9301 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9361 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9421 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9481 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9541 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9601 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9661 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9721 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9781 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9841 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9901 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9961 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tg
//
