LOCUS       sequence-272723-        7573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7573)
  AUTHORS   Moores SL, Selfors LM, Fredericks J, Breit T, Fujikawa K, Alt FW, 
            Brugge JS, Swat W
  TITLE     Vav family proteins couple to diverse cell surface receptors.
  JOURNAL   Mol Cell Biol. 2000 Sep . 20(17):6364-73.
  PUBMED    10938113
REFERENCE   2  (bases 1 to 7573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2000 Sep . 20(17):6364-73."
COMMENT     Sequence Label: pC.HA Vav3
FEATURES             Location/Qualifiers
     source          1..7573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           598..637
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             3214..3240
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          3272..3844
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(3976..3995)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4041..4096
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4095..4114)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      4450..4585
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4691..4708)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4862..5450)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4942..4961)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5621..6412,6413..6481))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6244..6263
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6482..6586)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6654..6672
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6710..6732)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6832..6851
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6918..7373)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7055..7076)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7267..7286
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7499..7521
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7513..7530
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7514..7530
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7537..7556
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7537..7555
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgggggat
      601 cttggtggcg tgaaactccc gcacctcttc ggccagcgcc ttgtagaagc gcgtatggct
      661 tctagaatgg agccgtggaa gcagtgcgct cagtggctca tccacagcaa ggtgctgccc
      721 cccaaccatc gggtgacctg ggactcggcg caggtgttcg acctggcgca gacccttcgc
      781 gatggagtcc tgctctgcca gctgctcaac aacctgcggc cacactccat caatctgaag
      841 gagatcaacc tgaggcccca gatgtcccag tttctctgtt tgaagaacat ccggacattc
      901 ctggccgcct gctgcgatac ctttggaatg aggaaaagtg aactttttga ggcatttgac
      961 ctatttgatg ttcgagactt tggaaaggtt atagaaacat tatctcggct atcccgaaca
     1021 ccaatagcat tggccacagg aatcaggccg ttccccactg aagaaagtat aaatgatgaa
     1081 gacatctaca aaggccttcc cgacttaata gatgaaaccc gcgtggaaga tgaagaagac
     1141 ctctatgact gtgtgtacgg cgaggatgaa ggcggagaag tctatgaaga cttgatgaag
     1201 gcggaggaag cccagcaacc taaatcacaa gaaaatgata tacggagttg ttgcctagca
     1261 gaaattaggc agacagaaga aaaatacaca gaaacgttgg agtcaataga aaaatatttc
     1321 atggcaccat tgaaaagatt tctgacggcg gcggaatttg attcagtatt tattaacatt
     1381 cctgaccttg taaaagttca tcggagctta atgcaagaga ttcatgattc cattgtaaat
     1441 aaagatgacc agaacttgta tcaagttttt attaactaca aggaaagatt ggttatttat
     1501 gggcagtact gcagtggagt ggagtcagcc atctctaacc tagactacat ctctaagaca
     1561 aaagaagatg tcaaactgaa attagaggaa tgttcaaaaa gagccaataa cgggaagttt
     1621 actctgcgcg atttgctggt cgttccaatg cagcgcgtgc tgaagtatca cctcctcctt
     1681 caggagctgg taaaacacac ccatgaccct atggagaagg cgaacctgaa gctggctctg
     1741 gatgcaatga aggacttggc acaatatgtg aatgaagtaa aaagagataa tgagaccctt
     1801 cgtgaaatta agcagtttca gctatctata gagaatttga accagccagt tttgctcttt
     1861 ggacgaccac agggagacgg tgaaatccga ataaccactc tggacaagca tacaaaacaa
     1921 gaaaggcata tcttcttatt cgacttggca gtgattgtct gtaaaaggaa aggcgataac
     1981 tacgagatga aggaaataat agatctccag cagtacaaga tagccaacaa ccctaccact
     2041 gacaaagaga ataaaaagtg gtcttatggc ttctacctca tccataccca aggacaaaat
     2101 gggttagaat tttattgcaa aacaaaagat ttaaagaaaa aatggctgga acagtttgaa
     2161 atggctctat ccaacataag gccagactat gcagactcca atttccatga tttcaagatg
     2221 cacaccttca ctcgggtgac atcctgcagg gtctgccaga tgctcctgag gggaacattt
     2281 tatcaaggct acttatgttt taagtgtgga gcaaaagcac ataaagaatg tttgggaaga
     2341 gtagacaatt gtggcagagt taattccgtt gaacaagggc cattcaaacc accagagaaa
     2401 cggaccaatg gacttcggag agcttccaga caggtggacc caggtttgcc gaagatgcag
     2461 gtcattagga actacactgg caccccggcc ccagggcttc atgaaggacc accattgcat
     2521 atccaggcag gcgacactgt tgaacttctg agaggagatg cacacagtgt gttctggcag
     2581 ggacgaaact tagcttcagg agaggttgga tttttcccaa gtgatgctgt caaaccctct
     2641 ccatgtgtac ctaaaccagt agattattct tgccaaccct ggtatgctgg cccaatggaa
     2701 agattgcagg cagaaactga acttattaac agggtgaata gtacttacct tgtaaggcac
     2761 aggaccaaag aatctggaga atatgcaatt agcattaagt acaataatga agcaaaacac
     2821 atcaagattt taacaagaga tggctttttt cacattgcag aaaatagaaa atttaaaagc
     2881 ttaatggaac ttgtggagta ctacaagcac cattctctca aagaagggtt caggacctta
     2941 gatacaacgc tacagtttcc atacaaggag ccagaacagc cagctggaca gaggggtaat
     3001 agaacaggca acagcttgtt gagtcccaaa gtactgggta ttgccatcgc tcggtacgac
     3061 ttctgtgcaa gagatatgcg ggagctctcc ctattgaaag gagacatggt gaagatttac
     3121 acgaagatga gtgcaaatgg ctggtggaga ggtgaagtaa atggcagggt gggctggttt
     3181 ccatccacat atgtggaaga agatgaaact agttatccgt acgacgtacc agactacgca
     3241 taagaaaagt gaggatcctg agaacttcag ggtgagtttg gggacccttg attgttcttt
     3301 ctttttcgct attgtaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt
     3361 tagaatggga agatgtccct tgtatcacca tggaccctca tgataatttt gtttctttca
     3421 ctttctactc tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt
     3481 ttcgttaaac tttagcttgc atttgtaacg aatttttaaa ttcacttttg tttatttgtc
     3541 agattgtaag tactttctct aatcactttt ttttcaaggc aatcagggta tattatattg
     3601 tacttcagca cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct
     3661 gcatataaat tctggctggc gtggaaatat tcttattggt agaaacaact acaccctggt
     3721 catcatcctg cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa
     3781 atactctgag tccaaaccgg gcccctctgc taaccatgtt catgccttct tctctttcct
     3841 acagctcctg ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattcac
     3901 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     3961 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     4021 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     4081 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     4141 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     4201 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
     4261 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
     4321 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
     4381 actactccca gtcatagctg tccctcttct cttatggaga tccctcgagg agctttttgc
     4441 aaaagcctag gcctccaaaa aagcctcttc actacttctg gaatagctca gaggccgagg
     4501 cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg
     4561 aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact
     4621 aattgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     4681 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     4741 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     4801 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     4861 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     4921 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     4981 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     5041 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     5101 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     5161 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     5221 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     5281 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     5341 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     5401 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     5461 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     5521 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     5581 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     5641 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     5701 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     5761 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     5821 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     5881 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     5941 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     6001 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     6061 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     6121 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     6181 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     6241 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     6301 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     6361 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     6421 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     6481 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     6541 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     6601 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     6661 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     6721 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     6781 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     6841 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     6901 gaaaataccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     6961 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     7021 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     7081 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     7141 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     7201 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     7261 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     7321 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg
     7381 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     7441 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     7501 cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa
     7561 ttcgagctcg ccc
//
