LOCUS       sequence-272749-        5517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression of CENP-T and CENP-W.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   McKinley KL, Sekulic N, Guo LY, Tsinman T, Black BE, Cheeseman IM
  TITLE     The CENP-L-N Complex Forms a Critical Node in an Integrated Meshwork
            of Interactions at the Centromere-Kinetochore Interface.
  JOURNAL   Mol Cell. 2015 Dec 17;60(6):886-98. doi: 
            10.1016/j.molcel.2015.10.027. Epub 2015 Nov 19.
  PUBMED    26698661
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.10.027"; journalName: "Mol Cell"; date: 
            "2015-12-17- 17"; volume: "60"; issue: "6"; pages: "886-98"
COMMENT     Sequence Label: pKM744
FEATURES             Location/Qualifiers
     source          1..5517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(16..21,22..38,39..44))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(115..148)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        225..316
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             362..382
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     polyA_signal    2281..2415
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2587..2678
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    3188..3322
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  3443..3608
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3798..4386
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4287..4306
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  4521..4744
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4811..5344)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 cgctacttgc attacagttt acgaaccgaa caggcttatg tcaactgggt tcgtgccttc
       61 atccgtttcc acggtgtgcg tcacccggca accttgggca gcagcgaagt cgccataact
      121 tcgtatagca tacattatac gaagttatct gtaactataa cggtcctaag gtagcgagtt
      181 taaacactag tatcgattcg cgacctactc cggaatatta atagatcatg gagataatta
      241 aaatgataac catctcgcaa ataaataagt attttactgt tttcgtaaca gttttgtaat
      301 aaaaaaacct ataaatattc cggattattc ataccgtccc accatcgggc gcggatccat
      361 gcatcatcat catcatcatc atggcagcgg cagcatggca gaccacaacc ctgacagcga
      421 ttctacacct cgtacactgc tgcgtcgtgt gctggatact gctgatcctc gtactcctcg
      481 tcgtcctcgt agcgctcgtg ctggtgctcg ccgtgccctg ctggaaacag cctctcctcg
      541 taaactgagt ggccagaccc gtactattgc ccgtgggcgt tcacatggag cccgctcggt
      601 cggtcgtagt gctcacattc aagcctcggg acacctggaa gaacaaaccc ctcgtaccct
      661 gctgaaaaac atcctgctga ctgcccctga aagctctatt ctgatgccgg aaagcgtcgt
      721 taaaccggtt cctgctcctc aagccgttca accttctcgt caagagtcct cctgtggatc
      781 tctggagctg caactgcctg aactggagcc tcctacaaca ctggcaccgg ggctgctggc
      841 gcctggtcgt cgtaaacaac gtctgcgtct gagcgtgttt caacaaggtg ttgatcaggg
      901 cctgagtctg tctcaagaac ctcaaggcaa cgctgatgct tctagtctga cccgttctct
      961 gaacctgacc tttgctactc cactgcaacc tcaaagcgtg caacgtcctg gactggctcg
     1021 ccgccctcct gctcgccgtg ccgttgatgt gggtgctttt ctgcgtgacc tgcgtgatac
     1081 aagcctggca cctccgaata ttgtgctgga ggatacacag ccgttttcac agcctatggt
     1141 tggtagccct aacgtttatc actcactgcc gtgtacccct cacactggtg ccgaagatgc
     1201 tgaacaagct gctggtcgta aaacacaaag ctctggaccg ggactgcaaa aaaactctcc
     1261 ggggaaacct gcccaattcc tggcgggtga agctgaagaa gtcaacgcct ttgctctggg
     1321 atttctgtct accagctcgg gtgttagcgg agaagatgag gtggaacctc tgcatgatgg
     1381 tgtggaagaa gccgagaaga aaatggaaga ggaaggggtg tctgtctctg aaatggaagc
     1441 gactggtgct caaggaccaa gtcgtgtcga agaggcagaa ggccataccg aggttaccga
     1501 agccgaagga agccaaggga cagctgaagc tgatggacct ggggcctcaa gtggggatga
     1561 agatgctagt ggccgtgccg cctcacctga atctgcttct agtacaccgg aaagcctgca
     1621 agcacgtcgt catcaccaat ttctggaacc tgctccggct cctggtgctg ctgttctgtc
     1681 tagtgaacct gctgaacctc tgctggttcg tcatccgcct cgtcctcgta caactggacc
     1741 acgtcctcgc caagaccctc ataaagcagg actgagccat tatgttaaac tgttctcgtt
     1801 ctatgccaaa atgccgatgg agcgtaaagc tctggaaatg gtcgaaaaat gcctggacaa
     1861 atatttccaa catctgtgtg acgatctgga agtgtttgcc gctcatgctg ggcgtaaaac
     1921 agtgaaacct gaggatctgg agctgctgat gcgtcgtcaa ggactggtta cggatcaagt
     1981 gtcgctgcat gtcctggtgg aacgtcatct gccgctggaa tatcgccaac tgctgattcc
     2041 ttgtgcttat agcgggaact ccgtctttcc tgcccagtaa gaattcaaag gcctacgtcg
     2101 acgagctcac ttgtcgcggc cgctttcgaa tctagagcct gcagtctcga caagcttgtc
     2161 gagaagtact agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta
     2221 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     2281 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     2341 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     2401 tatcatgtct ggatctgatc actgcttgag cctagaagat ccggctgcta acaaagcccg
     2461 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctatcataac ccctagggta
     2521 tacccatcta aggtagcgag tttaaacact agtatcgatt cgcgacctac tccggaatat
     2581 taatagatca tggagataat taaaatgata accatctcgc aaataaataa gtattttact
     2641 gttttcgtaa cagttttgta ataaaaaaac ctataaatat tccggattat tcataccgtc
     2701 ccaccatcgg gcgcggatcc atggcactga gcacaatcgt ttcacagcgt aaacaaatca
     2761 aacgtaaagc accggcaggt tttctggcag cggtattcaa acgtaaaaaa ccacagctgg
     2821 ccctggaaaa atctggtgat ctgctggttc atctgaattg cctgctgttt gttcaccgtc
     2881 tggcggaaga atctcgtacc aatgcctgtg cctcaaaatg tcgtgtcatc aataaagaac
     2941 acgtcctggc tgctgctaaa gtcatcctga aaaaatcggc gggctaagaa ttcaaaggcc
     3001 tacgtcgacg agctcacttg tcgcggccgc tttcgaatct agagcctgca gtctcgacaa
     3061 gcttgtcgag aagtactaga ggatcataat cagccatacc acatttgtag aggttttact
     3121 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     3181 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3241 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3301 tgtatcttat catgtctgga tctgatcact gcttgagcct agaagatccg gctgctaaca
     3361 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta tcataacccc
     3421 tagggtatac ccatctaatt ggaaccagat aagtgaaatc tagttccaaa ctattttgtc
     3481 atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat tttgtcactc
     3541 ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcccaa ctattttgtc
     3601 cgcccacaac cggttgactt gggtcaactg tcagaccaag tttactcata tatactttag
     3661 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     3721 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     3781 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     3841 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3901 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     3961 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4021 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4081 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4141 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     4201 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4261 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4321 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4381 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     4441 cacatgttct ttcctgcgtt atcccctgat tgacttgggt cgctcttcct gtggatgcgc
     4501 agatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa caaaatagat
     4561 ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga aatcagtcca
     4621 gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact cttcattttc
     4681 tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg taaagactat
     4741 attcgcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg atctcggctt
     4801 gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact tcttcccgta
     4861 tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct tgcacgtaga
     4921 tcacataagc accaagcgcg ttggcctcat gcttgaggag attgatgagc gcggtggcaa
     4981 tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc tcactacgcg
     5041 gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc aacaaccgct tcttggtcga
     5101 aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa tcggagtccg
     5161 gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga tcaagagcag
     5221 cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg cccatacttg
     5281 agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctgcgta
     5341 acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg
     5401 gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa aaccgccact
     5461 gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcata
//
