LOCUS       sequence-272755-        7967 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length UL87 3L_A with a C-terminal 2xStrep tag in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7967)
  AUTHORS   Castaneda AF, Didychuk AL, Louder RK, McCollum CO, Davis ZH, Nogales
            E, Glaunsinger BA
  TITLE     The gammaherpesviral TATA-box-binding protein directly interacts 
            with the CTD of host RNA Pol II to direct late gene transcription.
  JOURNAL   PLoS Pathog. 2020 Sep 4;16(9):e1008843. doi: 
            10.1371/journal.ppat.1008843. eCollection 2020 Sep.
  PUBMED    32886723
REFERENCE   2  (bases 1 to 7967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2020-09-4"; volume: "16(9):e1008843. doi"; pages: "
            10.1371/journal.ppat.1008843. eCollection 2020 Sep"
COMMENT     Sequence Label: pcDNA4/TO-UL87 3L_A-2xStrep
FEATURES             Location/Qualifiers
     source          1..7967
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3632..3655
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3692..3715
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(3741..3758)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3747..3971
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4017..4445
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4104..4123)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4314..4335
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4454..4474)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4459..4788
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4639..4774
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4701..4720
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4836..4883
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4902..5276
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5406..5527
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5443..5462)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5497..5516
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5576..5592)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5576..5592)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5589..5611)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5600..5616
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5624..5630,5631..5648,5649..5654))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5669..5690
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5807..5824)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5978..6566)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6058..6077)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6737..7528,7529..7597))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7360..7379
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7598..7702)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7777..7796)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt
      781 ggtggaattc atggccggcg ctgcgccgcg ccgcctcggc tgcgacgctc taatagtcgt
      841 cggcggctcc gctacgccgc gccgggtttt acacgtcccc gtgcacgttc gcgcctgcaa
      901 cctcacccaa gagctatcga cgggcgagga cgcccgcttc tgtcgtccgc gacccgttaa
      961 cgtcgaacgg gtacgcgctg ttttcgcggc tctctaccgt gcctgtcccg tacacgcgag
     1021 gaccgagtcc gagcgtgtca agctggtact gggtcgtgcc gcggcaggac ccgtggccgt
     1081 accctgtttt tgcgacggtg aagtggaggg ccacggcgag catctggtac ccacgacgca
     1141 gttttgtcgc gggccgctgc tctacgtgca ccgacgttgt tgttgcggat ccgtgaccgc
     1201 cgggcgcgcg ctgtcctacc acgttctcga aaaccacgtg gccacgcatg tgttacgcgg
     1261 attgctctcg ctgacggaat ggaatcgaga gttgccgggc cttttttgcg actgtcctgg
     1321 cagcggtggc gccttgggaa ccgaggaacg ctacgccatg gcctgcttgc cgcgcgacct
     1381 cagcctgcac ctggacgact atccttacct gatggtggaa atcggacgcg tactcagcgt
     1441 cagcgaggta gacgactacg taaccgccgt ctccggctac ctgggcgagg ccgcggcgcc
     1501 gcgcattcag gttcactaca agctgctctt tggactcaac gtgcgtccgc aagcgccgtg
     1561 cgcgttggac gctacacgcg acttttttct gctggagctg caaaagcttt ggctgggcgt
     1621 tgaatatcac cacgaagtca cgtcggagtt tttcggtcgc gtactggctc agctgcatcg
     1681 cgaccgcgcc cgcgtcatga tggcacttcg cttgcccgag cagacggtgt gccacctaag
     1741 caccttcgtt ctcagtcgct tcaagcgaca ggtactgtac ttcaagttac aggtgagcta
     1801 cggcaagtgc cggactggcc acgctgacag aagtggggga gggggaaacg gtggaagtca
     1861 gggacaccac aacctactgt gttatcgacg tcttagcgtc acgtttgccg acacggacac
     1921 ggtgtggaga aaccttttct acgtttatta tgaactagct cgggatctgg ggtcccatgg
     1981 gacagaggac cgatccgtaa gccgcggtta cggtgtttct tgcgctccga ggacgtcgcg
     2041 gctaccaccg tcagaaccga cggtggtttc agccaacgga cacgcgctgt cttccaccgc
     2101 gctcccgacg acgagcgcgg gtcacaagct gtcgctgccg cgcgacccgg ccgcagatcg
     2161 cgttcgacgt tacgtgtgca ttatctcgcg tctcatgttc gcccggtatg gggagagatg
     2221 gcgtaaacac cgtcgacggc ggtcggagac gggagaagag gaggaggaag agacggtgga
     2281 atcgggggag actgacgcca cgccgccatt tgactttacg gggcagcagc tgcgccgggc
     2341 ctatcaggaa caccgacgtc gtaaacatct agccgtgcag cgttacgcgc cgtgccgtcg
     2401 taagctcatc ggcgggatgg agtttgccga ggtgacgggc gttagtctgg accgcatcgc
     2461 cgtcaacgct ttcaacacca accgcgttat caatatgaag gccgcactct cgtccatcgc
     2521 cgcgtcgggt ctcggcgtgc gcgcgccgcg gcttcccaag aacatgaccc acagttttgt
     2581 gatgtacaag cacaccttca aggagcccgc ttgcaccgtc agcacttttg tttccaacga
     2641 cgccgtctac atcaactcgc tcaacgtcaa tattcgcggt tcctatcccg agtttctgta
     2701 ctcgctgggc gtgtaccggc tgcacgttaa tatcgatcac ttctttctgc cggccgtggt
     2761 gtgcaacagc aactcctcgc tggacgtgca tgggctggag gaccaggcgg tgatccgctc
     2821 ggagcgcagc aaggtgtact ggaccaccaa ctttccgtgc atgatctcgc atactaacaa
     2881 cgtcaacgtg ggttggttca aagcggctac ggccattgtg ccgcgcgtct cgggcgctga
     2941 cctggaagcc attctgctca aagaactctc gtgcatcaag aacatgcgcg acgtgtgcat
     3001 cgattacggt ctgcatcgcg ttttcacgca actagagctg cgcaattcgt accagatccc
     3061 cttcctggcc aagcagttgg tgctgtttct gcgtgcttgc ctgctcaagc tgcacggtcg
     3121 agagaagcgg ctgcagttgg accgcctagt atttgaggcg gcacagcggg gtctctttga
     3181 ctacagcaag aacctcacgg cgcacaccaa gatcaagcac acttgcgcgc tcatcggcag
     3241 tcgtctagcc aacaacgtgc ccaagatcct ggcccggaac aaaaaagtca aattggatca
     3301 cctgggccgg aacgccaacg tgctgacggt gtgtcggcac gtggaagccc acaagatccc
     3361 tcgcacgcgc ctcaaagtgt tagtcgaggt gctgggcgcg ttgcagagta tcagcggtac
     3421 gccgcacacg cgtgaagtga tccaccagac gttgtttcga ttgtgctcgg cggccgcagc
     3481 cacctcgggc ctgtgttcat cccctccccc attgtgtgtg tcctcatctt cctccgcccc
     3541 ttctgtccca acctccgtca gcgttgacgg cagttctgaa cccacgtcgc cgcgagcgcg
     3601 gtttgcatca cgactcgagg gaggcggtgg atggagccac ccccagttcg agaagggcgg
     3661 cggcagcggc ggcggcagcg gcggcggcag ctggagccac ccccagttcg agaaggggcc
     3721 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     3781 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     3841 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     3901 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     3961 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     4021 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4081 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4141 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     4201 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     4261 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     4321 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     4381 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4441 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     4501 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     4561 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     4621 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4681 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     4741 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     4801 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt
     4861 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg
     4921 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
     4981 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
     5041 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
     5101 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
     5161 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
     5221 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg
     5281 tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     5341 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     5401 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5461 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5521 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     5581 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     5641 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     5701 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     5761 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     5821 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     5881 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5941 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6001 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6061 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6121 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6181 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6241 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6301 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6361 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     6421 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6481 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6541 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6601 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6661 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6721 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6781 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6841 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     6901 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6961 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7021 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7081 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7141 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7201 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7261 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7321 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7381 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7441 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7501 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7561 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7621 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7681 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     7741 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
     7801 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
     7861 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
     7921 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgtt
//
