LOCUS       sequence-272780-        7854 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses chimeric STAT1-STAT3 protein with human STAT1 amino acids 
            1-508 joined to human STAT3 at amino acid 515, with a FLAG epitope 
            tag at the C-terminus. Based on addgene plasmids #8691 and #8707.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7854)
  AUTHORS   Horvath CM, Wen Z, Darnell JE Jr
  TITLE     A STAT protein domain that determines DNA sequence recognition 
            suggests a novel DNA-binding domain.
  JOURNAL   Genes Dev 1995 Apr 15;9(8):984-94.
  PUBMED    7774815
REFERENCE   2  (bases 1 to 7854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev";
            date: "1995-04-15"; volume: "9(8)"; pages: "984-94"
COMMENT     Sequence Label: pRcCMV-STAT13F
FEATURES             Location/Qualifiers
     source          1..7854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     635..654
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        635..653
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3049..3072
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(3085..3103)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3086..3103)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3123..3140)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3129..3353
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3399..3912
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3486..3505)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3696..3717
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3935..3955)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3940..4269
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4120..4255
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4336..5130
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4390..4409)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5000..5019
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5304..5438
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5341..5360)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5395..5414
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5469..5485)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5469..5485)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5482..5504)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5493..5509
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5517..5523,5524..5541,5542..5547))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5562..5583
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5700..5717)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5871..6459)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5951..5970)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6630..7421,7422..7490))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7253..7272
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7491..7595)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7670..7689)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa
      601 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
      661 aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg aattctgcag
      721 atatccatca cactggcggc cgcacaaggt ggcaggatgt ctcagtggta cgaacttcag
      781 cagcttgact caaaattcct ggagcaggtt caccagcttt atgatgacag ttttcccatg
      841 gaaatcagac agtacctggc acagtggtta gaaaagcaag actgggagca cgctgccaat
      901 gatgtttcat ttgccaccat ccgttttcat gacctcctgt cacagctgga tgatcaatat
      961 agtcgctttt ctttggagaa taacttcttg ctacagcata acataaggaa aagcaagcgt
     1021 aatcttcagg ataattttca ggaagaccca atccagatgt ctatgatcat ttacagctgt
     1081 ctgaaggaag aaaggaaaat tctggaaaac gcccagagat ttaatcaggc tcagtcgggg
     1141 aatattcaga gcacagtgat gttagacaaa cagaaagagc ttgacagtaa agtcagaaat
     1201 gtgaaggaca aggttatgtg tatagagcat gaaatcaaga gcctggaaga tttacaagat
     1261 gaatatgact tcaaatgcaa aaccttgcag aacagagaac acgagaccaa tggtgtggca
     1321 aagagtgatc agaaacaaga acagctgtta ctcaagaaga tgtatttaat gcttgacaat
     1381 aagagaaagg aagtagttca caaaataata gagttgctga atgtcactga acttacccag
     1441 aatgccctga ttaatgatga actagtggag tggaagcgga gacagcagag cgcctgtatt
     1501 ggggggccgc ccaatgcttg cttggatcag ctgcagaact ggttcactat agttgcggag
     1561 agtctgcagc aagttcggca gcagcttaaa aagttggagg aattggaaca gaaatacacc
     1621 tacgaacatg accctatcac aaaaaacaaa caagtgttat gggaccgcac cttcagtctt
     1681 ttccagcagc tcattcagag ctcgtttgtg gtggaaagac agccctgcat gccaacgcac
     1741 cctcagaggc cgctggtctt gaagacaggg gtccagttca ctgtgaagtt gagactgttg
     1801 gtgaaattgc aagagctgaa ttataatttg aaagtcaaag tcttatttga taaagatgtg
     1861 aatgagagaa atacagtaaa aggatttagg aagttcaaca ttttgggcac gcacacaaaa
     1921 gtgatgaaca tggaggagtc caccaatggc agtctggcgg ctgaatttcg gcacctgcaa
     1981 ttgaaagaac agaaaaatgc tggcaccaga acgaatgagg gtcctctcat cgttactgaa
     2041 gagcttcact cccttagttt tgaaacccaa ttgtgccagc ctggtttggt aattgacctc
     2101 gagacgacct ctctgcccgt tgtggtgatc tccaacgtca gccagctccc gagcggttgg
     2161 gcctccatcc tttggtacaa catgctggtg gcggaaccca ggaatctgtc cttcttcctg
     2221 actccaccat gtgcacgatg ggctcagctt tcagaagtgc tgagttggca gttctcgtcc
     2281 accaccaagc gagggctgag catcgagcag ctgacaacgc tggctgagaa gctcctaggg
     2341 cctggtgtga actactcagg gtgtcagatc acatgggcta aattctgcaa agaaaacatg
     2401 gctggcaagg gcttctcctt ctgggtctgg ctagacaata tcatcgacct tgtgaaaaag
     2461 tatatcttgg ccctttggaa tgaagggtac atcatgggtt tcatcagcaa ggagcgggag
     2521 cgggccatcc taagcacaaa gcccccgggc accttcctac tgcgcttcag cgagagcagc
     2581 aaagaaggag gggtcacttt cacttgggtg gaaaaggaca tcagtggcaa gacccagatc
     2641 cagtctgtag agccatacac caagcagcag ctgaacaaca tgtcatttgc tgaaatcatc
     2701 atgggctata agatcatgga tgcgaccaac atcctggtgt ctccacttgt ctacctctac
     2761 cccgacattc ccaaggagga ggcatttgga aagtactgta ggcccgagag ccaggagcac
     2821 cccgaagccg acccaggtag tgctgccccg tacctgaaga ccaagttcat ctgtgtgaca
     2881 ccaacgacct gcagcaatac cattgacctg ccgatgtccc cccgcacttt agattcattg
     2941 atgcagtttg gaaataacgg tgaaggtgct gagccctcag caggagggca gtttgagtcg
     3001 ctcacgtttg acatggattt gacctcggag tgtgctacct cccccatgga ctacaaagac
     3061 gatgacgata aatgagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc
     3121 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     3181 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     3241 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     3301 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     3361 ggcggaaaga accagctggg gctcgagggg ggatccccac gcgccctgta gcggcgcatt
     3421 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     3481 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     3541 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     3601 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     3661 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     3721 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     3781 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     3841 aacgcttaca atttaaatat ttgcttatac aatcttcctg tttttggggc ttttctgatt
     3901 atcaaccggg gtgggtaccg agctcgaatt ctgtggaatg tgtgtcagtt agggtgtgga
     3961 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     4021 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     4081 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     4141 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     4201 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     4261 ttttgcaaaa agctcccggg agcttggata tccattttcg gatctgatca agagacagga
     4321 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     4381 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     4441 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     4501 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     4561 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     4621 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     4681 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     4741 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     4801 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     4861 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     4921 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     4981 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     5041 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     5101 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     5161 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     5221 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     5281 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     5341 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     5401 tgtccaaact catcaatgta tcttatcatg tctggatccg tcgacctcga gagcttggcg
     5461 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     5521 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     5581 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     5641 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     5701 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     5761 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     5821 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     5881 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     5941 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6001 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6061 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6121 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6181 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6241 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6301 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6361 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6421 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     6481 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     6541 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     6601 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     6661 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     6721 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     6781 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     6841 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     6901 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     6961 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7021 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7081 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7141 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     7201 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     7261 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7321 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7381 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     7441 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     7501 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     7561 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7621 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     7681 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     7741 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     7801 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tata
//
