LOCUS       sequence-272789-       15111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iSTU-CRISPR/Cas9 with StIV2 intron, NU structure backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15111)
  AUTHORS   Zhong Z, Liu S, Liu X, Liu B, Tang X, Ren Q, Zhou J, Zheng X, Qi Y, 
            Zhang Y
  TITLE     Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome 
            Editing.
  JOURNAL   Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.1186/s12284-020-0369-8.
  PUBMED    32016614
REFERENCE   2  (bases 1 to 15111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Rice (N 
            Y)."; date: "2020-02-3"; volume: "13(1):8. doi"; pages: " 
            10.1186/s12284-020-0369-8"
COMMENT     Sequence Label: pGEL077
FEATURES             Location/Qualifiers
     source          1..15111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2005
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     misc_RNA        3505..3580
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             4030..6414
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(C)
                     /translation="CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILED
                     IVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGK
                     TILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKG
                     ILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQI
                     LKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDN
                     KVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDK
                     AGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMP
                     QVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKV
                     EKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELEN
                     GRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKY
                     FDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     CDS             6427..6447
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6458..6706
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    6727..6751
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8052..8681
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9110..10183
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10249..10443
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10679..10701)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10777..10917
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10832..10851
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10932..10949)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11103..11691)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11183..11202)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11778..12572)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12997..13021
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    13099..13273
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(14363..15039)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(14406..14426)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa ggtgagcatt
       61 gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca
      121 gtttatctat ctttatacat atatttaaac tttactctac gaataatata atctatagta
      181 ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag
      241 gacaattgag tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt
      301 ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt attagtacat
      361 ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac atctatttta
      421 ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata
      481 atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga
      541 aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa
      601 cgccgtcgac gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag
      661 cgaagcagac ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc
      721 caccgttgga cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg
      781 agccggcacg gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc
      841 tttcccaccg ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc
      901 acaccctctt tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagaactccc
      961 ccaaatccac ccgtcggcac ctccgcttca aggtacgccg ctcgtcctcc cccccccccn
     1021 tctctacctt ctcaagatcg gcgttccggt ccatggttag ggcccggtag ttctacttct
     1081 gttcatgttt gtgttagatc cgtgtttgtg ttagatccgt gctactagcg ttcgtacacg
     1141 gatgcgacct gtacgtcaga cacgttctga ttgctaactt gccagtgttt ctctttgggg
     1201 aatcctggga tggctctagc cgttccgcag acgggatcga tttcatgatt ttttttgttt
     1261 cgttgcatag ggtttggttt gcccttttcc tttatttcaa tatatgccgt gcacttgttt
     1321 gtcgggtcat cttttcatgc ttttttttgt cttggttgtg atgatgtggt ctggttgggc
     1381 ggtcgttcaa gatcggagta gaattaattc tgtttcaaac tacctggtgg atttattaat
     1441 tttggatctg tatgtgtgtg ccatacatat tcatagttac gaattgaaga tgatggatgg
     1501 aaatatcgat ctaggatagg tatacatgtt gatgcgggtt ttactgatgc atatacagag
     1561 atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt gggcggtcgt tcattcgttc
     1621 aagatcggag tagaatactg tttcaaacta cctggtgtat ttattaattt tggaactgta
     1681 tgtgtgtgtc atacatcttc atagttacga gtttaagatg gatggaaata tcgatctagg
     1741 ataggtatac atgttgatgt gggttttact gatgcatata catgatggca tatgcagcat
     1801 ctattcatat gctctaacct tgagtaccta tctattataa taaacaagta tgttttataa
     1861 ttattttgat cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt
     1921 tagccctgcc ttcatacgct atttatttgc ttggtactgt ttcttttgtc gatgctcacc
     1981 ctgttgtttg gtgttacttc tgcagcctgc aggtagatcg ctcgtcgaca tggataagaa
     2041 gtactctatc ggactcgata tcggaactaa ctctgtggga tgggctgtga tcaccgatga
     2101 gtacaaggtg ccatctaaga agttcaaggt tctcggaaac accgataggc actctatcaa
     2161 gaaaaacctt atcggtgctc tcctcttcga ttctggtgaa actgctgagg ctaccagact
     2221 caagagaacc gctagaagaa ggtacaccag aagaaagaac aggatctgct acctccaaga
     2281 gatcttctct aacgagatgg ctaaagtgga tgattcattc ttccacaggc tcgaagagtc
     2341 attcctcgtg gaagaagata agaagcacga gaggcaccct atcttcggaa acatcgttga
     2401 tgaggtggca taccacgaga agtaccctac tatctaccac ctcagaaaga agctcgttga
     2461 ttctactgat aaggctgatc tcaggctcat ctacctcgct ctcgctcaca tgatcaagtt
     2521 cagaggacac ttcctcatcg agggtgatct caaccctgat aactctgatg tggataagtt
     2581 gttcatccag ctcgtgcaga cctacaacca gcttttcgaa gagaacccta tcaacgcttc
     2641 aggtgtggat gctaaggcta tcctctctgc taggctctct aagtcaagaa ggcttgagaa
     2701 cctcattgct cagctccctg gtgagaagaa gaacggactt ttcggaaact tgatcgctct
     2761 ctctctcgga ctcaccccta acttcaagtc taacttcgat ctcgctgagg atgcaaagct
     2821 ccagctctca aaggatacct acgatgatga tctcgataac ctcctcgctc agatcggaga
     2881 tcagtacgct gatttgttcc tcgctgctaa gaacctctct gatgctatcc tcctcagtga
     2941 tatcctcaga gtgaacaccg agatcaccaa ggctccactc tcagcttcta tgatcaagag
     3001 atacgatgag caccaccagg atctcacact tctcaaggct cttgttagac agcagctccc
     3061 agagaagtac aaagagattt tcttcgatca gtctaagaac ggatacgctg gttacatcga
     3121 tggtggtgca tctcaagaag agttctacaa gttcatcaag cctatcctcg agaagatgga
     3181 tggaaccgag gaactcctcg tgaagctcaa tagagaggat cttctcagaa agcagaggac
     3241 cttcgataac ggatctatcc ctcatcagat ccacctcgga gagttgcacg ctatccttag
     3301 aaggcaagag gatttctacc cattcctcaa ggataacagg gaaaagattg agaagattct
     3361 caccttcaga atcccttact acgtgggacc tctcgctaga ggaaactcaa gattcgcttg
     3421 gatgaccaga aagtctgagg aaaccatcac cccttggaac ttcgaagagg taagtttctg
     3481 cttctaccgg agaccggggt ctcagtttta gagctagaaa tagcaagtta aaataaggct
     3541 agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttgatatat atataataat
     3601 tatcattaat tagtagtaat ataatatttc aaatattttt ttcaaaataa aagaatgtag
     3661 tatatagcaa ttgcttttct gtagtttata agtgtgtata ttttaattta taacttttct
     3721 aatatatgac caaaatttgt tgatgtgcag gtggtggata agggtgctag tgctcagtct
     3781 ttcatcgaga ggatgaccaa cttcgataag aaccttccaa acgagaaggt gctccctaag
     3841 cactctttgc tctacgagta cttcaccgtg tacaacgagt tgaccaaggt taagtacgtg
     3901 accgagggaa tgaggaagcc tgcttttttg tcaggtgagc aaaagaaggc tatcgttgat
     3961 ctcttgttca agaccaacag aaaggtgacc gtgaagcagc tcaaagagga ttacttcaag
     4021 aaaatcgagt gcttcgattc agttgagatt tctggtgttg aggataggtt caacgcatct
     4081 ctcggaacct accacgatct cctcaagatc attaaggata aggatttctt ggataacgag
     4141 gaaaacgagg atatcttgga ggatatcgtt cttaccctca ccctctttga agatagagag
     4201 atgattgaag aaaggctcaa gacctacgct catctcttcg atgataaggt gatgaagcag
     4261 ttgaagagaa gaagatacac tggttgggga aggctctcaa gaaagctcat taacggaatc
     4321 agggataagc agtctggaaa gacaatcctt gatttcctca agtctgatgg attcgctaac
     4381 agaaacttca tgcagctcat ccacgatgat tctctcacct ttaaagagga tatccagaag
     4441 gctcaggttt caggacaggg tgatagtctc catgagcata tcgctaacct cgctggatct
     4501 cctgcaatca agaagggaat cctccagact gtgaaggttg tggatgagtt ggtgaaggtg
     4561 atgggaaggc ataagcctga gaacatcgtg atcgaaatgg ctagagagaa ccagaccact
     4621 cagaagggac agaagaactc tagggaaagg atgaagagga tcgaggaagg tatcaaagag
     4681 cttggatctc agatcctcaa agagcaccct gttgagaaca ctcagctcca gaatgagaag
     4741 ctctacctct actacctcca gaacggaagg gatatgtatg tggatcaaga gttggatatc
     4801 aacaggctct ctgattacga tgttgatcat atcgtgccac agtcattctt gaaggatgat
     4861 tctatcgata acaaggtgct caccaggtct gataagaaca ggggtaagag tgataacgtg
     4921 ccaagtgaag aggttgtgaa gaaaatgaag aactattgga ggcagctcct caacgctaag
     4981 ctcatcactc agagaaagtt cgataacttg actaaggctg agaggggagg actctctgaa
     5041 ttggataagg caggattcat caagaggcag cttgtggaaa ccaggcagat cactaagcac
     5101 gttgcacaga tcctcgattc taggatgaac accaagtacg atgagaacga taagttgatc
     5161 agggaagtga aggttatcac cctcaagtca aagctcgtgt ctgatttcag aaaggatttc
     5221 caattctaca aggtgaggga aatcaacaac taccaccacg ctcacgatgc ttaccttaac
     5281 gctgttgttg gaaccgctct catcaagaag tatcctaagc tcgagtcaga gttcgtgtac
     5341 ggtgattaca aggtgtacga tgtgaggaag atgatcgcta agtctgagca agagatcgga
     5401 aaggctaccg ctaagtattt cttctactct aacatcatga atttcttcaa gaccgagatt
     5461 accctcgcta acggtgagat cagaaagagg ccactcatcg agacaaacgg tgaaacaggt
     5521 gagatcgtgt gggataaggg aagggatttc gctaccgtta gaaaggtgct ctctatgcca
     5581 caggtgaaca tcgttaagaa aaccgaggtg cagaccggtg gattctctaa agagtctatc
     5641 ctccctaaga ggaactctga taagctcatt gctaggaaga aggattggga ccctaagaaa
     5701 tacggtggtt tcgattctcc taccgtggct tactctgttc tcgttgtggc taaggttgag
     5761 aagggaaaga gtaagaagct caagtctgtt aaggaacttc tcggaatcac tatcatggaa
     5821 aggtcatctt tcgagaagaa cccaatcgat ttcctcgagg ctaagggata caaagaggtt
     5881 aagaaggatc tcatcatcaa gctcccaaag tactcactct tcgaactcga gaacggtaga
     5941 aagaggatgc tcgcttctgc tggtgagctt caaaagggaa acgagcttgc tctcccatct
     6001 aagtacgtta actttcttta cctcgcttct cactacgaga agttgaaggg atctccagaa
     6061 gataacgagc agaagcaact tttcgttgag cagcacaagc actacttgga tgagatcatc
     6121 gagcagatct ctgagttctc taaaagggtg atcctcgctg atgcaaacct cgataaggtg
     6181 ttgtctgctt acaacaagca cagagataag cctatcaggg aacaggcaga gaacatcatc
     6241 catctcttca cccttaccaa cctcggtgct cctgctgctt tcaagtactt cgatacaacc
     6301 atcgatagga agagatacac ctctaccaaa gaagtgctcg atgctaccct catccatcag
     6361 tctatcactg gactctacga gactaggatc gatctctcac agctcggtgg tgattcaagg
     6421 gctgatccta agaagaagag gaaggtttga ctcgagatat gaagatgaag atgaaatatt
     6481 tggtgtgtca aataaaaagc ttgtgtgctt aagtttgtgt ttttttcttg gcttgttgtg
     6541 ttatgaattt gtggcttttt ctaatattaa atgaatgtaa gatcacatta taatgaataa
     6601 acaaatgttt ctataatcca ttgtgaatgt tttgttggat ctcttctgca gcatataact
     6661 actgtatgtg ctatggtatg gactatggaa tatgattaaa gataaggagc tcaaactatc
     6721 agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa
     6781 tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca
     6841 accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc
     6901 agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta
     6961 agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag
     7021 tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc
     7081 cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca
     7141 acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac
     7201 caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt
     7261 gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga
     7321 gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac
     7381 cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc
     7441 cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt
     7501 tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca
     7561 ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta
     7621 ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt
     7681 ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga
     7741 ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag
     7801 atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cggctcaacc
     7861 gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc
     7921 agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa
     7981 aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc
     8041 aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga
     8101 ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg
     8161 attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa
     8221 ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg
     8281 acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg
     8341 cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg
     8401 acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct
     8461 ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg
     8521 ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca
     8581 ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg
     8641 tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa
     8701 aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc
     8761 tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc
     8821 ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga
     8881 gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta
     8941 gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg
     9001 ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg
     9061 cctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg
     9121 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt
     9181 gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc
     9241 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc
     9301 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct
     9361 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc
     9421 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga
     9481 ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc
     9541 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg
     9601 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg
     9661 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat
     9721 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt
     9781 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga
     9841 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt
     9901 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg
     9961 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg
    10021 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga
    10081 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg
    10141 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct
    10201 agggcaaatt gccctagcag gggaaaaagg tcgaaaaggc ctctttcctg tggatagcac
    10261 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa
    10321 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga
    10381 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc
    10441 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct
    10501 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat
    10561 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga
    10621 ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
    10681 tctgacacat gcagctcccg gaaacggtca cagcttgtct gtaagcggat gccgggagca
    10741 gacaagcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
    10801 cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg
    10861 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
    10921 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    10981 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    11041 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    11101 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    11161 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    11221 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    11281 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    11341 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    11401 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    11461 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    11521 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
    11581 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    11641 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    11701 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    11761 gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc
    11821 caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata
    11881 tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt
    11941 ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc
    12001 aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac
    12061 agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc
    12121 agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag
    12181 ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata
    12241 atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca
    12301 ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc
    12361 agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct
    12421 ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat
    12481 accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt
    12541 ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct tttctacagt
    12601 atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg
    12661 cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt
    12721 ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca gcaacgctct
    12781 gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag
    12841 cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac
    12901 ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt
    12961 gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa
    13021 caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt actgaattaa
    13081 cgccgaatta attcggggga tctggatttt agtactggat tttggtttta ggaattagaa
    13141 attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc ttatatgctc
    13201 aacacatgag cgaaacccta taggaaccct aattccctta tctgggaact actcacacat
    13261 tattatggag aaaatagagc ttgtcgatcg ttaattaatc acctccaaga tcccggacga
    13321 gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc ggtccagacg
    13381 gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt
    13441 gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac caagctctga
    13501 tagagttggt caagaccaat gcggagcata tacgcccgga gccgcggcga tcctgcaagc
    13561 tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc
    13621 cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc gctccagtca
    13681 atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa atccgcgtgc
    13741 acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc gagagcctgc
    13801 gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca
    13861 gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg cggtccgaat
    13921 gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat ggcctccgcg
    13981 accggctgca gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt
    14041 gcacggcggg agatgcaata ggtcaggctc tcgctgaatt ccccaatgtc aagcacttcc
    14101 ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt gtagaaacca
    14161 tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa gctgaaagca
    14221 cgagattctt cgccctccga gagctgcatc aggtcggaca cgctgtcgaa cttttcgatc
    14281 agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatcctagg agagatagat
    14341 ttgtagagag agactggtga tttcagcgtg tcctctccaa atgaaatgaa cttccttata
    14401 tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga
    14461 tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc
    14521 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag
    14581 cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt
    14641 tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt
    14701 gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt
    14761 agacgagagt gtcgtgctcc accatgttca catcaatcca cttgctttga agacgtggtt
    14821 ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg
    14881 tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg
    14941 ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg
    15001 aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag
    15061 actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat g
//
