LOCUS       sequence-272814-        8212 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8212)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 8212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: HRas (G12V, E37G)-pcw107-V5
FEATURES             Location/Qualifiers
     source          1..8212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..516
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     435..453
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    537..561
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(579..611,612..614,615..1145)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIGDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     protein_bind    complement(1147..1171)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1179..1220
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1245..1833
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1298..1318)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1716..1727)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1905..2138
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2216..2337
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2253..2272)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2307..2326
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2377..2512
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(2533..2551)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2561..2578)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2561..2577)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2570..2592)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2719..3174
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2806..2825)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3016..3037
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3200..3304
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3305..3373,3374..4165)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3523..3542)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4336..4924
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4825..4844
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5078..5095
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5212..5233
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5248..5253,5254..5271,5272..5278)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5286..5302
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5291..5313
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5310..5326
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5310..5326
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5345..5365
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5347..5365
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5393..5619
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5620..5800
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5847..5972
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6465..6698
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6883..6927
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7158..7368
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7180..7376
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7227..7362
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7408..8010
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(7408..7427)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7904..7924
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8053..8170
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc
       61 tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc
      121 acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg
      181 cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga
      241 caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg
      301 gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag
      361 agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg
      421 ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc
      481 tccctcgttg accgaatcac cgacctctct ccccaggggc tagcatcgat ggatcaacaa
      541 gtttgtacaa aaaagttggc ttccgcggcg gcggcaccat gacggaatat aagctggtgg
      601 tggtgggcgc cgtcggtgtg ggcaagagtg cgctgaccat ccagctgatc cagaaccatt
      661 ttgtggacga atacgacccc actatagggg attcctaccg gaagcaggtg gtcattgatg
      721 gggagacgtg cctgttggac atcctggata ccgccggcca ggaggagtac agcgccatgc
      781 gggaccagta catgcgcacc ggggagggct tcctgtgtgt gtttgccatc aacaacacca
      841 agtcttttga ggacatccac cagtacaggg agcagatcaa acgggtgaag gactcggatg
      901 acgtgcccat ggtgctggtg gggaacaagt gtgacctggc tgcacgcact gtggaatctc
      961 ggcaggctca ggacctcgcc cgaagctacg gcatccccta catcgagacc tcggccaaga
     1021 cccggcaggg agtggaggat gccttctaca cgttggtgcg tgagatccgg cagcacaagc
     1081 tgcggaagct gaaccctcct gatgagagtg gccccggctg catgagctgc aagtgtgtgc
     1141 tctccttgcc aactttcttg tacaaagtgg ttaccggtgg taagcctatc cctaaccctc
     1201 tcctcggtct cgattctacg tagtaatgac cgcgttaagt cgacaatcaa cctctggatt
     1261 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     1321 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     1381 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     1441 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     1501 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     1561 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     1621 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     1681 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     1741 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     1801 cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa
     1861 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     1921 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     1981 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     2041 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     2101 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca
     2161 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     2221 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     2281 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     2341 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     2401 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     2461 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     2521 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     2581 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     2641 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     2701 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     2761 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     2821 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     2881 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     2941 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     3001 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     3061 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     3121 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     3181 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3241 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3301 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     3361 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     3421 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     3481 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     3541 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     3601 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     3661 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     3721 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     3781 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     3841 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     3901 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     3961 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     4021 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     4081 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     4141 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     4201 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     4261 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4321 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4381 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4441 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     4501 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4561 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4621 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4681 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4741 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4801 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4861 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4921 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4981 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     5041 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     5101 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     5161 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     5221 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     5281 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     5341 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
     5401 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
     5461 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
     5521 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
     5581 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
     5641 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     5701 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     5761 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     5821 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     5881 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     5941 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     6001 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     6061 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     6121 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     6181 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     6241 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     6301 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     6361 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     6421 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     6481 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     6541 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     6601 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     6661 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     6721 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     6781 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     6841 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     6901 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     6961 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     7021 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     7081 ctcccaaccc cgaggggacc catgcattgc atctcaatta gtcagcaacc aggtgtggaa
     7141 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcgtctcaat tagtcagcaa
     7201 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     7261 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     7321 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     7381 tttctagagg taccaccgga gcttaccatg accgagtaca agcccacggt gcgcctcgcc
     7441 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     7501 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     7561 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     7621 gccgtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     7681 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     7741 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     7801 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     7861 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     7921 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     7981 tgcatgaccc gcaagcccgg tgccagttaa cgaattctag atcttgagac aaatggcagt
     8041 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     8101 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     8161 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc gg
//
