LOCUS       sequence-272833-       15888 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iSTU-CRISPR/Cas9 with StIV2 intron, tRNA structure backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15888)
  AUTHORS   Zhong Z, Liu S, Liu X, Liu B, Tang X, Ren Q, Zhou J, Zheng X, Qi Y, 
            Zhang Y
  TITLE     Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome 
            Editing.
  JOURNAL   Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.1186/s12284-020-0369-8.
  PUBMED    32016614
REFERENCE   2  (bases 1 to 15888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Rice (N 
            Y)."; date: "2020-02-3"; volume: "13(1):8. doi"; pages: " 
            10.1186/s12284-020-0369-8"
COMMENT     Sequence Label: pGEL078
FEATURES             Location/Qualifiers
     source          1..15888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2005
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             complement(3573..3878)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(3675..3694)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_RNA        4205..4280
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             4807..7191
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(C)
                     /translation="CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILED
                     IVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGK
                     TILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKG
                     ILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQI
                     LKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDN
                     KVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDK
                     AGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMP
                     QVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKV
                     EKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELEN
                     GRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKY
                     FDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     CDS             7204..7224
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7235..7483
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    7504..7528
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8829..9458
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9887..10960
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11026..11220
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11456..11478)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11554..11694
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11609..11628
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11709..11726)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11880..12468)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11960..11979)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12555..13349)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    13774..13798
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    13876..14050
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(15140..15816)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(15183..15203)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa ggtgagcatt
       61 gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca
      121 gtttatctat ctttatacat atatttaaac tttactctac gaataatata atctatagta
      181 ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag
      241 gacaattgag tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt
      301 ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt attagtacat
      361 ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac atctatttta
      421 ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata
      481 atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga
      541 aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa
      601 cgccgtcgac gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag
      661 cgaagcagac ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc
      721 caccgttgga cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg
      781 agccggcacg gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc
      841 tttcccaccg ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc
      901 acaccctctt tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagaactccc
      961 ccaaatccac ccgtcggcac ctccgcttca aggtacgccg ctcgtcctnc cccccccccc
     1021 tctctacctt ctcaagatcg gcgttccggt ccatggttag ggcccggtag ttctacttct
     1081 gttcatgttt gtgttagatc cgtgtttgtg ttagatccgt gctactagcg ttcgtacacg
     1141 gatgcgacct gtacgtcaga cacgttctga ttgctaactt gccagtgttt ctctttgggg
     1201 aatcctggga tggctctagc cgttccgcag acgggatcga tttcatgatt ttttttgttt
     1261 cgttgcatag ggtttggttt gcccttttcc tttatttcaa tatatgccgt gcacttgttt
     1321 gtcgggtcat cttttcatgc ttttttttgt cttggttgtg atgatgtggt ctggttgggc
     1381 ggtcgttcaa gatcggagta gaattaattc tgtttcaaac tacctggtgg atttattaat
     1441 tttggatctg tatgtgtgtg ccatacatat tcatagttac gaattgaaga tgatggatgg
     1501 aaatatcgat ctaggatagg tatacatgtt gatgcgggtt ttactgatgc atatacagag
     1561 atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt gggcggtcgt tcattcgttc
     1621 aagatcggag tagaatactg tttcaaacta cctggtgtat ttattaattt tggaactgta
     1681 tgtgtgtgtc atacatcttc atagttacga gtttaagatg gatggaaata tcgatctagg
     1741 ataggtatac atgttgatgt gggttttact gatgcatata catgatggca tatgcagcat
     1801 ctattcatat gctctaacct tgagtaccta tctattataa taaacaagta tgttttataa
     1861 ttattttgat cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt
     1921 tagccctgcc ttcatacgct atttatttgc ttggtactgt ttcttttgtc gatgctcacc
     1981 ctgttgtttg gtgttacttc tgcagcctgc aggtagatcg ctcgtcgaca tggataagaa
     2041 gtactctatc ggactcgata tcggaactaa ctctgtggga tgggctgtga tcaccgatga
     2101 gtacaaggtg ccatctaaga agttcaaggt tctcggaaac accgataggc actctatcaa
     2161 gaaaaacctt atcggtgctc tcctcttcga ttctggtgaa actgctgagg ctaccagact
     2221 caagagaacc gctagaagaa ggtacaccag aagaaagaac aggatctgct acctccaaga
     2281 gatcttctct aacgagatgg ctaaagtgga tgattcattc ttccacaggc tcgaagagtc
     2341 attcctcgtg gaagaagata agaagcacga gaggcaccct atcttcggaa acatcgttga
     2401 tgaggtggca taccacgaga agtaccctac tatctaccac ctcagaaaga agctcgttga
     2461 ttctactgat aaggctgatc tcaggctcat ctacctcgct ctcgctcaca tgatcaagtt
     2521 cagaggacac ttcctcatcg agggtgatct caaccctgat aactctgatg tggataagtt
     2581 gttcatccag ctcgtgcaga cctacaacca gcttttcgaa gagaacccta tcaacgcttc
     2641 aggtgtggat gctaaggcta tcctctctgc taggctctct aagtcaagaa ggcttgagaa
     2701 cctcattgct cagctccctg gtgagaagaa gaacggactt ttcggaaact tgatcgctct
     2761 ctctctcgga ctcaccccta acttcaagtc taacttcgat ctcgctgagg atgcaaagct
     2821 ccagctctca aaggatacct acgatgatga tctcgataac ctcctcgctc agatcggaga
     2881 tcagtacgct gatttgttcc tcgctgctaa gaacctctct gatgctatcc tcctcagtga
     2941 tatcctcaga gtgaacaccg agatcaccaa ggctccactc tcagcttcta tgatcaagag
     3001 atacgatgag caccaccagg atctcacact tctcaaggct cttgttagac agcagctccc
     3061 agagaagtac aaagagattt tcttcgatca gtctaagaac ggatacgctg gttacatcga
     3121 tggtggtgca tctcaagaag agttctacaa gttcatcaag cctatcctcg agaagatgga
     3181 tggaaccgag gaactcctcg tgaagctcaa tagagaggat cttctcagaa agcagaggac
     3241 cttcgataac ggatctatcc ctcatcagat ccacctcgga gagttgcacg ctatccttag
     3301 aaggcaagag gatttctacc cattcctcaa ggataacagg gaaaagattg agaagattct
     3361 caccttcaga atcccttact acgtgggacc tctcgctaga ggaaactcaa gattcgcttg
     3421 gatgaccaga aagtctgagg aaaccatcac cccttggaac ttcgaagagg taagtttctg
     3481 cttctaccaa caaagcacca gtggtctagt ggtagaatag taccctgcca cggtacagac
     3541 ccgggttcga ttcccggctg gtgcaggaga ccttatattc cccagaacat caggttaatg
     3601 gcgtttttga tgtcattttc gcggtggctg agatcagcca cttcttcccc gataacggaa
     3661 accggcacac tggccatatc ggtggtcatc atgcgccagc tttcatcccc gatatgcacc
     3721 accgggtaaa gttcacggga gactttatct gacagcagac gtgcactggc cagggggatc
     3781 accatccgtc gcccgggcgt gtcaataata tcactctgta catccacaaa cagacgataa
     3841 cggctctctc ttttataggt gtaaacctta aactgcattt caccagcccc tgttctcgtc
     3901 agcaaaagag ccgttcattt caataaaccg tgcgacctca gccatccctt cctgattttc
     3961 cgctttccag cgttcggcac gcagacgacg ggcttcattc tgcatggttg tgcttaccag
     4021 accggagata ttgacatcat atatgccttg agcaactgat agctgtcgct gtcaactgtc
     4081 actgtaatac gctgcttcat agcatacctc tttttgacat acttcgggta tacatatcag
     4141 tatatattct tataccgcaa aaatcagcgc gcaaatacgc atactgttat ctggcttggt
     4201 ctcagtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa
     4261 aagtggcacc gagtcggtgc aacaaagcac cagtggtcta gtggtagaat agtaccctgc
     4321 cacggtacag acccgggttc gattcccggc tggtgcattt gatatatata taataattat
     4381 cattaattag tagtaatata atatttcaaa tatttttttc aaaataaaag aatgtagtat
     4441 atagcaattg cttttctgta gtttataagt gtgtatattt taatttataa cttttctaat
     4501 atatgaccaa aatttgttga tgtgcaggtg gtggataagg gtgctagtgc tcagtctttc
     4561 atcgagagga tgaccaactt cgataagaac cttccaaacg agaaggtgct ccctaagcac
     4621 tctttgctct acgagtactt caccgtgtac aacgagttga ccaaggttaa gtacgtgacc
     4681 gagggaatga ggaagcctgc ttttttgtca ggtgagcaaa agaaggctat cgttgatctc
     4741 ttgttcaaga ccaacagaaa ggtgaccgtg aagcagctca aagaggatta cttcaagaaa
     4801 atcgagtgct tcgattcagt tgagatttct ggtgttgagg ataggttcaa cgcatctctc
     4861 ggaacctacc acgatctcct caagatcatt aaggataagg atttcttgga taacgaggaa
     4921 aacgaggata tcttggagga tatcgttctt accctcaccc tctttgaaga tagagagatg
     4981 attgaagaaa ggctcaagac ctacgctcat ctcttcgatg ataaggtgat gaagcagttg
     5041 aagagaagaa gatacactgg ttggggaagg ctctcaagaa agctcattaa cggaatcagg
     5101 gataagcagt ctggaaagac aatccttgat ttcctcaagt ctgatggatt cgctaacaga
     5161 aacttcatgc agctcatcca cgatgattct ctcaccttta aagaggatat ccagaaggct
     5221 caggtttcag gacagggtga tagtctccat gagcatatcg ctaacctcgc tggatctcct
     5281 gcaatcaaga agggaatcct ccagactgtg aaggttgtgg atgagttggt gaaggtgatg
     5341 ggaaggcata agcctgagaa catcgtgatc gaaatggcta gagagaacca gaccactcag
     5401 aagggacaga agaactctag ggaaaggatg aagaggatcg aggaaggtat caaagagctt
     5461 ggatctcaga tcctcaaaga gcaccctgtt gagaacactc agctccagaa tgagaagctc
     5521 tacctctact acctccagaa cggaagggat atgtatgtgg atcaagagtt ggatatcaac
     5581 aggctctctg attacgatgt tgatcatatc gtgccacagt cattcttgaa ggatgattct
     5641 atcgataaca aggtgctcac caggtctgat aagaacaggg gtaagagtga taacgtgcca
     5701 agtgaagagg ttgtgaagaa aatgaagaac tattggaggc agctcctcaa cgctaagctc
     5761 atcactcaga gaaagttcga taacttgact aaggctgaga ggggaggact ctctgaattg
     5821 gataaggcag gattcatcaa gaggcagctt gtggaaacca ggcagatcac taagcacgtt
     5881 gcacagatcc tcgattctag gatgaacacc aagtacgatg agaacgataa gttgatcagg
     5941 gaagtgaagg ttatcaccct caagtcaaag ctcgtgtctg atttcagaaa ggatttccaa
     6001 ttctacaagg tgagggaaat caacaactac caccacgctc acgatgctta ccttaacgct
     6061 gttgttggaa ccgctctcat caagaagtat cctaagctcg agtcagagtt cgtgtacggt
     6121 gattacaagg tgtacgatgt gaggaagatg atcgctaagt ctgagcaaga gatcggaaag
     6181 gctaccgcta agtatttctt ctactctaac atcatgaatt tcttcaagac cgagattacc
     6241 ctcgctaacg gtgagatcag aaagaggcca ctcatcgaga caaacggtga aacaggtgag
     6301 atcgtgtggg ataagggaag ggatttcgct accgttagaa aggtgctctc tatgccacag
     6361 gtgaacatcg ttaagaaaac cgaggtgcag accggtggat tctctaaaga gtctatcctc
     6421 cctaagagga actctgataa gctcattgct aggaagaagg attgggaccc taagaaatac
     6481 ggtggtttcg attctcctac cgtggcttac tctgttctcg ttgtggctaa ggttgagaag
     6541 ggaaagagta agaagctcaa gtctgttaag gaacttctcg gaatcactat catggaaagg
     6601 tcatctttcg agaagaaccc aatcgatttc ctcgaggcta agggatacaa agaggttaag
     6661 aaggatctca tcatcaagct cccaaagtac tcactcttcg aactcgagaa cggtagaaag
     6721 aggatgctcg cttctgctgg tgagcttcaa aagggaaacg agcttgctct cccatctaag
     6781 tacgttaact ttctttacct cgcttctcac tacgagaagt tgaagggatc tccagaagat
     6841 aacgagcaga agcaactttt cgttgagcag cacaagcact acttggatga gatcatcgag
     6901 cagatctctg agttctctaa aagggtgatc ctcgctgatg caaacctcga taaggtgttg
     6961 tctgcttaca acaagcacag agataagcct atcagggaac aggcagagaa catcatccat
     7021 ctcttcaccc ttaccaacct cggtgctcct gctgctttca agtacttcga tacaaccatc
     7081 gataggaaga gatacacctc taccaaagaa gtgctcgatg ctaccctcat ccatcagtct
     7141 atcactggac tctacgagac taggatcgat ctctcacagc tcggtggtga ttcaagggct
     7201 gatcctaaga agaagaggaa ggtttgactc gagatatgaa gatgaagatg aaatatttgg
     7261 tgtgtcaaat aaaaagcttg tgtgcttaag tttgtgtttt tttcttggct tgttgtgtta
     7321 tgaatttgtg gctttttcta atattaaatg aatgtaagat cacattataa tgaataaaca
     7381 aatgtttcta taatccattg tgaatgtttt gttggatctc ttctgcagca tataactact
     7441 gtatgtgcta tggtatggac tatggaatat gattaaagat aaggagctca aactatcagt
     7501 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
     7561 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
     7621 acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt
     7681 cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt
     7741 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg
     7801 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc
     7861 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg
     7921 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag
     7981 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac
     8041 agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg
     8101 catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac
     8161 gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct
     8221 aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg
     8281 cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga
     8341 aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg
     8401 cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg
     8461 tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga
     8521 acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc
     8581 gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgg ctcaaccgtg
     8641 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
     8701 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
     8761 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
     8821 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
     8881 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
     8941 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
     9001 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
     9061 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
     9121 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
     9181 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
     9241 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
     9301 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
     9361 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
     9421 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
     9481 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
     9541 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
     9601 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
     9661 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
     9721 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
     9781 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgcct
     9841 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
     9901 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
     9961 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
    10021 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc
    10081 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
    10141 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
    10201 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
    10261 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
    10321 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
    10381 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
    10441 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
    10501 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
    10561 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
    10621 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca
    10681 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
    10741 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
    10801 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
    10861 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
    10921 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
    10981 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggcctc tttcctgtgg atagcacgta
    11041 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
    11101 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
    11161 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
    11221 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg
    11281 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc
    11341 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg
    11401 ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
    11461 gacacatgca gctcccggaa acggtcacag cttgtctgta agcggatgcc gggagcagac
    11521 aagcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
    11581 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
    11641 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
    11701 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
    11761 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
    11821 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    11881 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    11941 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    12001 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    12061 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    12121 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    12181 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    12241 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    12301 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
    12361 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    12421 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    12481 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    12541 atgcattcta ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa
    12601 tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc
    12661 tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc
    12721 ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg
    12781 tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc
    12841 tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga
    12901 tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga
    12961 caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc
    13021 ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc
    13081 atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc
    13141 tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta
    13201 taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc
    13261 ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc
    13321 aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt
    13381 taaagatacc ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat
    13441 tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata
    13501 acatagtatc gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc
    13561 atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt
    13621 agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc
    13681 tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc
    13741 gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa
    13801 attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc
    13861 cgaattaatt cgggggatct ggattttagt actggatttt ggttttagga attagaaatt
    13921 ttattgatag aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac
    13981 acatgagcga aaccctatag gaaccctaat tcccttatct gggaactact cacacattat
    14041 tatggagaaa atagagcttg tcgatcgtta attaatcacc tccaagatcc cggacgagtg
    14101 ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc
    14161 gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg
    14221 tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag
    14281 agttggtcaa gaccaatgcg gagcatatac gcccggagcc gcggcgatcc tgcaagctcc
    14341 ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag
    14401 aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg
    14461 accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg
    14521 aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg
    14581 acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca
    14641 atcgcgcata tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg
    14701 ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccatggc ctccgcgacc
    14761 ggctgcagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca
    14821 cggcgggaga tgcaataggt caggctctcg ctgaattccc caatgtcaag cacttccgga
    14881 atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg
    14941 gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga
    15001 gattcttcgc cctccgagag ctgcatcagg tcggacacgc tgtcgaactt ttcgatcaga
    15061 aacttctcga cagacgtcgc ggtgagttca ggctttttca tcctaggaga gatagatttg
    15121 tagagagaga ctggtgattt cagcgtgtcc tctccaaatg aaatgaactt ccttatatag
    15181 aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat
    15241 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
    15301 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct
    15361 ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga
    15421 tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt
    15481 gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga
    15541 cgagagtgtc gtgctccacc atgttcacat caatccactt gctttgaaga cgtggttgga
    15601 acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg
    15661 gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca
    15721 ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg
    15781 aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact
    15841 gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatg
//
