LOCUS       sequence-272834-       15182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iSTU-CRISPR/Cas9 with StIV2 intron, RZ structure backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15182)
  AUTHORS   Zhong Z, Liu S, Liu X, Liu B, Tang X, Ren Q, Zhou J, Zheng X, Qi Y, 
            Zhang Y
  TITLE     Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome 
            Editing.
  JOURNAL   Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.1186/s12284-020-0369-8.
  PUBMED    32016614
REFERENCE   2  (bases 1 to 15182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Rice (N 
            Y)."; date: "2020-02-3"; volume: "13(1):8. doi"; pages: " 
            10.1186/s12284-020-0369-8"
COMMENT     Sequence Label: pGEL079
FEATURES             Location/Qualifiers
     source          1..15182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2005
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     misc_RNA        3520..3595
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             4101..6485
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(C)
                     /translation="CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILED
                     IVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGK
                     TILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKG
                     ILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQI
                     LKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDN
                     KVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDK
                     AGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMP
                     QVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKV
                     EKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELEN
                     GRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKY
                     FDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     CDS             6498..6518
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6529..6777
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    6798..6822
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8123..8752
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9181..10254
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10320..10514
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10750..10772)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10848..10988
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10903..10922
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11003..11020)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11174..11762)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11254..11273)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11849..12643)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    13068..13092
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    13170..13344
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(14434..15110)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(14477..14497)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa ggtgagcatt
       61 gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca
      121 gtttatctat ctttatacat atatttaaac tttactctac gaataatata atctatagta
      181 ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag
      241 gacaattgag tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt
      301 ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt attagtacat
      361 ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac atctatttta
      421 ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata
      481 atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga
      541 aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa
      601 cgccgtcgac gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag
      661 cgaagcagac ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc
      721 caccgttgga cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg
      781 agccggcacg gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc
      841 tttcccaccg ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc
      901 acaccctctt tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagaactccc
      961 ccaaatccac ccgtcggcac ctccgcttca aggtacgccg ctcgtcctcc cccccccccn
     1021 tctctacctt ctcaagatcg gcgttccggt ccatggttag ggcccggtag ttctacttct
     1081 gttcatgttt gtgttagatc cgtgtttgtg ttagatccgt gctactagcg ttcgtacacg
     1141 gatgcgacct gtacgtcaga cacgttctga ttgctaactt gccagtgttt ctctttgggg
     1201 aatcctggga tggctctagc cgttccgcag acgggatcga tttcatgatt ttttttgttt
     1261 cgttgcatag ggtttggttt gcccttttcc tttatttcaa tatatgccgt gcacttgttt
     1321 gtcgggtcat cttttcatgc ttttttttgt cttggttgtg atgatgtggt ctggttgggc
     1381 ggtcgttcaa gatcggagta gaattaattc tgtttcaaac tacctggtgg atttattaat
     1441 tttggatctg tatgtgtgtg ccatacatat tcatagttac gaattgaaga tgatggatgg
     1501 aaatatcgat ctaggatagg tatacatgtt gatgcgggtt ttactgatgc atatacagag
     1561 atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt gggcggtcgt tcattcgttc
     1621 aagatcggag tagaatactg tttcaaacta cctggtgtat ttattaattt tggaactgta
     1681 tgtgtgtgtc atacatcttc atagttacga gtttaagatg gatggaaata tcgatctagg
     1741 ataggtatac atgttgatgt gggttttact gatgcatata catgatggca tatgcagcat
     1801 ctattcatat gctctaacct tgagtaccta tctattataa taaacaagta tgttttataa
     1861 ttattttgat cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt
     1921 tagccctgcc ttcatacgct atttatttgc ttggtactgt ttcttttgtc gatgctcacc
     1981 ctgttgtttg gtgttacttc tgcagcctgc aggtagatcg ctcgtcgaca tggataagaa
     2041 gtactctatc ggactcgata tcggaactaa ctctgtggga tgggctgtga tcaccgatga
     2101 gtacaaggtg ccatctaaga agttcaaggt tctcggaaac accgataggc actctatcaa
     2161 gaaaaacctt atcggtgctc tcctcttcga ttctggtgaa actgctgagg ctaccagact
     2221 caagagaacc gctagaagaa ggtacaccag aagaaagaac aggatctgct acctccaaga
     2281 gatcttctct aacgagatgg ctaaagtgga tgattcattc ttccacaggc tcgaagagtc
     2341 attcctcgtg gaagaagata agaagcacga gaggcaccct atcttcggaa acatcgttga
     2401 tgaggtggca taccacgaga agtaccctac tatctaccac ctcagaaaga agctcgttga
     2461 ttctactgat aaggctgatc tcaggctcat ctacctcgct ctcgctcaca tgatcaagtt
     2521 cagaggacac ttcctcatcg agggtgatct caaccctgat aactctgatg tggataagtt
     2581 gttcatccag ctcgtgcaga cctacaacca gcttttcgaa gagaacccta tcaacgcttc
     2641 aggtgtggat gctaaggcta tcctctctgc taggctctct aagtcaagaa ggcttgagaa
     2701 cctcattgct cagctccctg gtgagaagaa gaacggactt ttcggaaact tgatcgctct
     2761 ctctctcgga ctcaccccta acttcaagtc taacttcgat ctcgctgagg atgcaaagct
     2821 ccagctctca aaggatacct acgatgatga tctcgataac ctcctcgctc agatcggaga
     2881 tcagtacgct gatttgttcc tcgctgctaa gaacctctct gatgctatcc tcctcagtga
     2941 tatcctcaga gtgaacaccg agatcaccaa ggctccactc tcagcttcta tgatcaagag
     3001 atacgatgag caccaccagg atctcacact tctcaaggct cttgttagac agcagctccc
     3061 agagaagtac aaagagattt tcttcgatca gtctaagaac ggatacgctg gttacatcga
     3121 tggtggtgca tctcaagaag agttctacaa gttcatcaag cctatcctcg agaagatgga
     3181 tggaaccgag gaactcctcg tgaagctcaa tagagaggat cttctcagaa agcagaggac
     3241 cttcgataac ggatctatcc ctcatcagat ccacctcgga gagttgcacg ctatccttag
     3301 aaggcaagag gatttctacc cattcctcaa ggataacagg gaaaagattg agaagattct
     3361 caccttcaga atcccttact acgtgggacc tctcgctaga ggaaactcaa gattcgcttg
     3421 gatgaccaga aagtctgagg aaaccatcac cccttggaac ttcgaagagg taagtttctg
     3481 cttctaccgc ttttgtcacc ggaggagacc ggggtctcag ttttagagct agaaatagca
     3541 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttg
     3601 tcaccggatg tgttttccgg tctgatgagt ccgtgaggac gaaacaaaag ctttgatata
     3661 tatataataa ttatcattaa ttagtagtaa tataatattt caaatatttt tttcaaaata
     3721 aaagaatgta gtatatagca attgcttttc tgtagtttat aagtgtgtat attttaattt
     3781 ataacttttc taatatatga ccaaaatttg ttgatgtgca ggtggtggat aagggtgcta
     3841 gtgctcagtc tttcatcgag aggatgacca acttcgataa gaaccttcca aacgagaagg
     3901 tgctccctaa gcactctttg ctctacgagt acttcaccgt gtacaacgag ttgaccaagg
     3961 ttaagtacgt gaccgaggga atgaggaagc ctgctttttt gtcaggtgag caaaagaagg
     4021 ctatcgttga tctcttgttc aagaccaaca gaaaggtgac cgtgaagcag ctcaaagagg
     4081 attacttcaa gaaaatcgag tgcttcgatt cagttgagat ttctggtgtt gaggataggt
     4141 tcaacgcatc tctcggaacc taccacgatc tcctcaagat cattaaggat aaggatttct
     4201 tggataacga ggaaaacgag gatatcttgg aggatatcgt tcttaccctc accctctttg
     4261 aagatagaga gatgattgaa gaaaggctca agacctacgc tcatctcttc gatgataagg
     4321 tgatgaagca gttgaagaga agaagataca ctggttgggg aaggctctca agaaagctca
     4381 ttaacggaat cagggataag cagtctggaa agacaatcct tgatttcctc aagtctgatg
     4441 gattcgctaa cagaaacttc atgcagctca tccacgatga ttctctcacc tttaaagagg
     4501 atatccagaa ggctcaggtt tcaggacagg gtgatagtct ccatgagcat atcgctaacc
     4561 tcgctggatc tcctgcaatc aagaagggaa tcctccagac tgtgaaggtt gtggatgagt
     4621 tggtgaaggt gatgggaagg cataagcctg agaacatcgt gatcgaaatg gctagagaga
     4681 accagaccac tcagaaggga cagaagaact ctagggaaag gatgaagagg atcgaggaag
     4741 gtatcaaaga gcttggatct cagatcctca aagagcaccc tgttgagaac actcagctcc
     4801 agaatgagaa gctctacctc tactacctcc agaacggaag ggatatgtat gtggatcaag
     4861 agttggatat caacaggctc tctgattacg atgttgatca tatcgtgcca cagtcattct
     4921 tgaaggatga ttctatcgat aacaaggtgc tcaccaggtc tgataagaac aggggtaaga
     4981 gtgataacgt gccaagtgaa gaggttgtga agaaaatgaa gaactattgg aggcagctcc
     5041 tcaacgctaa gctcatcact cagagaaagt tcgataactt gactaaggct gagaggggag
     5101 gactctctga attggataag gcaggattca tcaagaggca gcttgtggaa accaggcaga
     5161 tcactaagca cgttgcacag atcctcgatt ctaggatgaa caccaagtac gatgagaacg
     5221 ataagttgat cagggaagtg aaggttatca ccctcaagtc aaagctcgtg tctgatttca
     5281 gaaaggattt ccaattctac aaggtgaggg aaatcaacaa ctaccaccac gctcacgatg
     5341 cttaccttaa cgctgttgtt ggaaccgctc tcatcaagaa gtatcctaag ctcgagtcag
     5401 agttcgtgta cggtgattac aaggtgtacg atgtgaggaa gatgatcgct aagtctgagc
     5461 aagagatcgg aaaggctacc gctaagtatt tcttctactc taacatcatg aatttcttca
     5521 agaccgagat taccctcgct aacggtgaga tcagaaagag gccactcatc gagacaaacg
     5581 gtgaaacagg tgagatcgtg tgggataagg gaagggattt cgctaccgtt agaaaggtgc
     5641 tctctatgcc acaggtgaac atcgttaaga aaaccgaggt gcagaccggt ggattctcta
     5701 aagagtctat cctccctaag aggaactctg ataagctcat tgctaggaag aaggattggg
     5761 accctaagaa atacggtggt ttcgattctc ctaccgtggc ttactctgtt ctcgttgtgg
     5821 ctaaggttga gaagggaaag agtaagaagc tcaagtctgt taaggaactt ctcggaatca
     5881 ctatcatgga aaggtcatct ttcgagaaga acccaatcga tttcctcgag gctaagggat
     5941 acaaagaggt taagaaggat ctcatcatca agctcccaaa gtactcactc ttcgaactcg
     6001 agaacggtag aaagaggatg ctcgcttctg ctggtgagct tcaaaaggga aacgagcttg
     6061 ctctcccatc taagtacgtt aactttcttt acctcgcttc tcactacgag aagttgaagg
     6121 gatctccaga agataacgag cagaagcaac ttttcgttga gcagcacaag cactacttgg
     6181 atgagatcat cgagcagatc tctgagttct ctaaaagggt gatcctcgct gatgcaaacc
     6241 tcgataaggt gttgtctgct tacaacaagc acagagataa gcctatcagg gaacaggcag
     6301 agaacatcat ccatctcttc acccttacca acctcggtgc tcctgctgct ttcaagtact
     6361 tcgatacaac catcgatagg aagagataca cctctaccaa agaagtgctc gatgctaccc
     6421 tcatccatca gtctatcact ggactctacg agactaggat cgatctctca cagctcggtg
     6481 gtgattcaag ggctgatcct aagaagaaga ggaaggtttg actcgagata tgaagatgaa
     6541 gatgaaatat ttggtgtgtc aaataaaaag cttgtgtgct taagtttgtg tttttttctt
     6601 ggcttgttgt gttatgaatt tgtggctttt tctaatatta aatgaatgta agatcacatt
     6661 ataatgaata aacaaatgtt tctataatcc attgtgaatg ttttgttgga tctcttctgc
     6721 agcatataac tactgtatgt gctatggtat ggactatgga atatgattaa agataaggag
     6781 ctcaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt
     6841 tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta
     6901 tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc
     6961 tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg
     7021 cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc
     7081 gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg
     7141 aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac
     7201 ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag
     7261 atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct
     7321 ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg
     7381 gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg
     7441 gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag
     7501 ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga
     7561 ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag
     7621 ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc
     7681 tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg
     7741 cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat
     7801 gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca
     7861 ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc
     7921 acggctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg
     7981 cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg
     8041 tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata
     8101 aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc
     8161 aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt
     8221 tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga
     8281 tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat
     8341 cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc
     8401 cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg
     8461 ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct
     8521 acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc
     8581 cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag
     8641 ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc
     8701 tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga
     8761 ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc
     8821 agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac
     8881 tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag
     8941 accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga
     9001 ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc
     9061 aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag
     9121 cggctgggtt gcctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc
     9181 gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg
     9241 gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc
     9301 cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg
     9361 gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc
     9421 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt
     9481 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac
     9541 gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg
     9601 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga
     9661 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga
     9721 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg
     9781 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag
     9841 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac
     9901 atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac
     9961 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac
    10021 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac
    10081 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc
    10141 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc
    10201 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg
    10261 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg cctctttcct
    10321 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt
    10381 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag
    10441 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc
    10501 ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc
    10561 cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc
    10621 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg
    10681 tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga
    10741 cggtgaaaac ctctgacaca tgcagctccc ggaaacggtc acagcttgtc tgtaagcgga
    10801 tgccgggagc agacaagcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
    10861 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
    10921 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
    10981 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
    11041 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
    11101 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    11161 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    11221 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    11281 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    11341 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    11401 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    11461 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    11521 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    11581 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
    11641 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    11701 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    11761 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    11821 aagggatttt ggtcatgcat tctaggtact aaaacaattc atccagtaaa atataatatt
    11881 ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt
    11941 cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg
    12001 ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt
    12061 tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta
    12121 aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat
    12181 ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc
    12241 tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat
    12301 acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc
    12361 catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct
    12421 ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat
    12481 aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca
    12541 ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt
    12601 cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc
    12661 ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga actccaattc
    12721 actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc
    12781 aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc
    12841 agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc
    12901 aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc
    12961 cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt
    13021 ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat
    13081 tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg
    13141 tactgaatta acgccgaatt aattcggggg atctggattt tagtactgga ttttggtttt
    13201 aggaattaga aattttattg atagaagtat tttacaaata caaatacata ctaagggttt
    13261 cttatatgct caacacatga gcgaaaccct ataggaaccc taattccctt atctgggaac
    13321 tactcacaca ttattatgga gaaaatagag cttgtcgatc gttaattaat cacctccaag
    13381 atcccggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat
    13441 cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg
    13501 atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa
    13561 ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg
    13621 atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca
    13681 accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct
    13741 cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga
    13801 aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat
    13861 cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca
    13921 catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt
    13981 gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca
    14041 tggcctccgc gaccggctgc agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg
    14101 tgacaccctg tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat tccccaatgt
    14161 caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt
    14221 tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga
    14281 agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggac acgctgtcga
    14341 acttttcgat cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatcctag
    14401 gagagataga tttgtagaga gagactggtg atttcagcgt gtcctctcca aatgaaatga
    14461 acttccttat atagaggaag ggtcttgcga aggatagtgg gattgtgcgt catcccttac
    14521 gtcagtggag atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt
    14581 ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc
    14641 ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc
    14701 tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga
    14761 tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat
    14821 attcttggag tagacgagag tgtcgtgctc caccatgttc acatcaatcc acttgctttg
    14881 aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt
    14941 tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc
    15001 atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc
    15061 aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt
    15121 ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca
    15181 tg
//
