LOCUS       sequence-272835-       15989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iSTU-CRISPR/Cas9 with OsCDPK2 intron, tRNA structure backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15989)
  AUTHORS   Zhong Z, Liu S, Liu X, Liu B, Tang X, Ren Q, Zhou J, Zheng X, Qi Y, 
            Zhang Y
  TITLE     Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome 
            Editing.
  JOURNAL   Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.1186/s12284-020-0369-8.
  PUBMED    32016614
REFERENCE   2  (bases 1 to 15989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Rice (N 
            Y)."; date: "2020-02-3"; volume: "13(1):8. doi"; pages: " 
            10.1186/s12284-020-0369-8"
COMMENT     Sequence Label: pGEL081
FEATURES             Location/Qualifiers
     source          1..15989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2005
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             complement(3575..3880)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYQVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(3677..3696)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_RNA        4207..4282
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             4908..7292
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(C)
                     /translation="CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILED
                     IVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGK
                     TILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKG
                     ILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQI
                     LKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDN
                     KVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDK
                     AGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMP
                     QVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKV
                     EKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELEN
                     GRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKY
                     FDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     CDS             7305..7325
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7336..7584
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    7605..7629
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8930..9559
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9988..11061
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11127..11321
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11557..11579)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11655..11795
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11710..11729
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11810..11827)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11981..12569)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12061..12080)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12656..13450)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    13875..13899
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    13977..14151
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(15241..15917)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(15284..15304)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa ggtgagcatt
       61 gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca
      121 gtttatctat ctttatacat atatttaaac tttactctac gaataatata atctatagta
      181 ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag
      241 gacaattgag tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt
      301 ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt attagtacat
      361 ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac atctatttta
      421 ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata
      481 atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga
      541 aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa
      601 cgccgtcgac gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag
      661 cgaagcagac ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc
      721 caccgttgga cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg
      781 agccggcacg gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc
      841 tttcccaccg ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc
      901 acaccctctt tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagaactccc
      961 ccaaatccac ccgtcggcac ctccgcttca aggtacgccg ctcgtcctcc cccccccccc
     1021 tctctacctt ctcaagatcg gcgttccggt ccatggttag ggcccggtag ttctacttct
     1081 gttcatgttt gtgttagatc cgtgtttgtg ttagatccgt gctactagcg ttcgtacacg
     1141 gatgcgacct gtacgtcaga cacgttctga ttgctaactt gccagtgttt ctctttgggg
     1201 aatcctggga tggctctagc cgttccgcag acgggatcga tttcatgatt ttttttgttt
     1261 cgttgcatag ggtttggttt gcccttttcc tttatttcaa tatatgccgt gcacttgttt
     1321 gtcgggtcat cttttcatgc ttttttttgt cttggttgtg atgatgtggt ctggttgggc
     1381 ggtcgttcaa gatcggagta gaattaattc tgtttcaaac tacctggtgg atttattaat
     1441 tttggatctg tatgtgtgtg ccatacatat tcatagttac gaattgaaga tgatggatgg
     1501 aaatatcgat ctaggatagg tatacatgtt gatgcgggtt ttactgatgc atatacagag
     1561 atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt gggcggtcgt tcattcgttc
     1621 aagatcggag tagaatactg tttcaaacta cctggtgtat ttattaattt tggaactgta
     1681 tgtgtgtgtc atacatcttc atagttacga gtttaagatg gatggaaata tcgatctagg
     1741 ataggtatac atgttgatgt gggttttact gatgcatata catgatggca tatgcagcat
     1801 ctattcatat gctctaacct tgagtaccta tctattataa taaacaagta tgttttataa
     1861 ttattttgat cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt
     1921 tagccctgcc ttcatacgct atttatttgc ttggtactgt ttcttttgtc gatgctcacc
     1981 ctgttgtttg gtgttacttc tgcagcctgc aggtagatcg ctcgtcgaca tggataagaa
     2041 gtactctatc ggactcgata tcggaactaa ctctgtggga tgggctgtga tcaccgatga
     2101 gtacaaggtg ccatctaaga agttcaaggt tctcggaaac accgataggc actctatcaa
     2161 gaaaaacctt atcggtgctc tcctcttcga ttctggtgaa actgctgagg ctaccagact
     2221 caagagaacc gctagaagaa ggtacaccag aagaaagaac aggatctgct acctccaaga
     2281 gatcttctct aacgagatgg ctaaagtgga tgattcattc ttccacaggc tcgaagagtc
     2341 attcctcgtg gaagaagata agaagcacga gaggcaccct atcttcggaa acatcgttga
     2401 tgaggtggca taccacgaga agtaccctac tatctaccac ctcagaaaga agctcgttga
     2461 ttctactgat aaggctgatc tcaggctcat ctacctcgct ctcgctcaca tgatcaagtt
     2521 cagaggacac ttcctcatcg agggtgatct caaccctgat aactctgatg tggataagtt
     2581 gttcatccag ctcgtgcaga cctacaacca gcttttcgaa gagaacccta tcaacgcttc
     2641 aggtgtggat gctaaggcta tcctctctgc taggctctct aagtcaagaa ggcttgagaa
     2701 cctcattgct cagctccctg gtgagaagaa gaacggactt ttcggaaact tgatcgctct
     2761 ctctctcgga ctcaccccta acttcaagtc taacttcgat ctcgctgagg atgcaaagct
     2821 ccagctctca aaggatacct acgatgatga tctcgataac ctcctcgctc agatcggaga
     2881 tcagtacgct gatttgttcc tcgctgctaa gaacctctct gatgctatcc tcctcagtga
     2941 tatcctcaga gtgaacaccg agatcaccaa ggctccactc tcagcttcta tgatcaagag
     3001 atacgatgag caccaccagg atctcacact tctcaaggct cttgttagac agcagctccc
     3061 agagaagtac aaagagattt tcttcgatca gtctaagaac ggatacgctg gttacatcga
     3121 tggtggtgca tctcaagaag agttctacaa gttcatcaag cctatcctcg agaagatgga
     3181 tggaaccgag gaactcctcg tgaagctcaa tagagaggat cttctcagaa agcagaggac
     3241 cttcgataac ggatctatcc ctcatcagat ccacctcgga gagttgcacg ctatccttag
     3301 aaggcaagag gatttctacc cattcctcaa ggataacagg gaaaagattg agaagattct
     3361 caccttcaga atcccttact acgtgggacc tctcgctaga ggaaactcaa gattcgcttg
     3421 gatgaccaga aagtctgagg aaaccatcac cccttggaac ttcgaagagg tcaatatgct
     3481 gtcaaaggca aacaaagcac cagtggtcta gtggtagaat agtaccctgc cacggtacag
     3541 acccgggttc gattcccggc tggtgcagga gaccttatat tccccagaac atcaggttaa
     3601 tggcgttttt gatgtcattt tcgcggtggc tgagatcagc cacttcttcc ccgataacgg
     3661 aaaccggcac actggccata tcggtggtca tcatgcgcca gctttcatcc ccgatatgca
     3721 ccacctggta aagttcacgg gagactttat ctgacagcag acgtgcactg gccaggggga
     3781 tcaccatccg tcgcccgggc gtgtcaataa tatcactctg tacatccaca aacagacgat
     3841 aacggctctc tcttttatag gtgtaaacct taaactgcat ttcaccagcc cctgttctcg
     3901 tcagcaaaag agccgttcat ttcaataaac cgggcgacct cagccatccc ttcctgattt
     3961 tccgctttcc agcgttcggc acgcagacga cgggcttcat tctgcatggt tgtgcttacc
     4021 agaccggaga tattgacatc atatatgcct tgagcaactg atagctgtcg ctgtcaactg
     4081 tcactgtaat acgctgcttc atagcatacc tctttttgac atacttcggg tatacatatc
     4141 agtatatatt cttataccgc aaaaatcagc gcgcaaatac gcatactgtt atctggcttg
     4201 gtctcagttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
     4261 aaaagtggca ccgagtcggt gcaacaaagc accagtggtc tagtggtaga atagtaccct
     4321 gccacggtac agacccgggt tcgattcccg gctggtgcat ttcttttctt ttccttttcg
     4381 ttgcttgttt tccccttctg atgttgtgtg ttgagtttat tgtttggttt gctggtagat
     4441 ccagtatttg gatcattttt cctttttttt ttctttcatt gcttctgcgg agggatgagt
     4501 ttgtgcatga atcttttgtt ggttggattc gcttagtttt cacactagtc ctttgattca
     4561 gcgcaaatct ttgttaattg gctcgcttaa ttctgacata aagtgttcct tcttggttct
     4621 gcttgcaggt ggtggataag ggtgctagtg ctcagtcttt catcgagagg atgaccaact
     4681 tcgataagaa ccttccaaac gagaaggtgc tccctaagca ctctttgctc tacgagtact
     4741 tcaccgtgta caacgagttg accaaggtta agtacgtgac cgagggaatg aggaagcctg
     4801 cttttttgtc aggtgagcaa aagaaggcta tcgttgatct cttgttcaag accaacagaa
     4861 aggtgaccgt gaagcagctc aaagaggatt acttcaagaa aatcgagtgc ttcgattcag
     4921 ttgagatttc tggtgttgag gataggttca acgcatctct cggaacctac cacgatctcc
     4981 tcaagatcat taaggataag gatttcttgg ataacgagga aaacgaggat atcttggagg
     5041 atatcgttct taccctcacc ctctttgaag atagagagat gattgaagaa aggctcaaga
     5101 cctacgctca tctcttcgat gataaggtga tgaagcagtt gaagagaaga agatacactg
     5161 gttggggaag gctctcaaga aagctcatta acggaatcag ggataagcag tctggaaaga
     5221 caatccttga tttcctcaag tctgatggat tcgctaacag aaacttcatg cagctcatcc
     5281 acgatgattc tctcaccttt aaagaggata tccagaaggc tcaggtttca ggacagggtg
     5341 atagtctcca tgagcatatc gctaacctcg ctggatctcc tgcaatcaag aagggaatcc
     5401 tccagactgt gaaggttgtg gatgagttgg tgaaggtgat gggaaggcat aagcctgaga
     5461 acatcgtgat cgaaatggct agagagaacc agaccactca gaagggacag aagaactcta
     5521 gggaaaggat gaagaggatc gaggaaggta tcaaagagct tggatctcag atcctcaaag
     5581 agcaccctgt tgagaacact cagctccaga atgagaagct ctacctctac tacctccaga
     5641 acggaaggga tatgtatgtg gatcaagagt tggatatcaa caggctctct gattacgatg
     5701 ttgatcatat cgtgccacag tcattcttga aggatgattc tatcgataac aaggtgctca
     5761 ccaggtctga taagaacagg ggtaagagtg ataacgtgcc aagtgaagag gttgtgaaga
     5821 aaatgaagaa ctattggagg cagctcctca acgctaagct catcactcag agaaagttcg
     5881 ataacttgac taaggctgag aggggaggac tctctgaatt ggataaggca ggattcatca
     5941 agaggcagct tgtggaaacc aggcagatca ctaagcacgt tgcacagatc ctcgattcta
     6001 ggatgaacac caagtacgat gagaacgata agttgatcag ggaagtgaag gttatcaccc
     6061 tcaagtcaaa gctcgtgtct gatttcagaa aggatttcca attctacaag gtgagggaaa
     6121 tcaacaacta ccaccacgct cacgatgctt accttaacgc tgttgttgga accgctctca
     6181 tcaagaagta tcctaagctc gagtcagagt tcgtgtacgg tgattacaag gtgtacgatg
     6241 tgaggaagat gatcgctaag tctgagcaag agatcggaaa ggctaccgct aagtatttct
     6301 tctactctaa catcatgaat ttcttcaaga ccgagattac cctcgctaac ggtgagatca
     6361 gaaagaggcc actcatcgag acaaacggtg aaacaggtga gatcgtgtgg gataagggaa
     6421 gggatttcgc taccgttaga aaggtgctct ctatgccaca ggtgaacatc gttaagaaaa
     6481 ccgaggtgca gaccggtgga ttctctaaag agtctatcct ccctaagagg aactctgata
     6541 agctcattgc taggaagaag gattgggacc ctaagaaata cggtggtttc gattctccta
     6601 ccgtggctta ctctgttctc gttgtggcta aggttgagaa gggaaagagt aagaagctca
     6661 agtctgttaa ggaacttctc ggaatcacta tcatggaaag gtcatctttc gagaagaacc
     6721 caatcgattt cctcgaggct aagggataca aagaggttaa gaaggatctc atcatcaagc
     6781 tcccaaagta ctcactcttc gaactcgaga acggtagaaa gaggatgctc gcttctgctg
     6841 gtgagcttca aaagggaaac gagcttgctc tcccatctaa gtacgttaac tttctttacc
     6901 tcgcttctca ctacgagaag ttgaagggat ctccagaaga taacgagcag aagcaacttt
     6961 tcgttgagca gcacaagcac tacttggatg agatcatcga gcagatctct gagttctcta
     7021 aaagggtgat cctcgctgat gcaaacctcg ataaggtgtt gtctgcttac aacaagcaca
     7081 gagataagcc tatcagggaa caggcagaga acatcatcca tctcttcacc cttaccaacc
     7141 tcggtgctcc tgctgctttc aagtacttcg atacaaccat cgataggaag agatacacct
     7201 ctaccaaaga agtgctcgat gctaccctca tccatcagtc tatcactgga ctctacgaga
     7261 ctaggatcga tctctcacag ctcggtggtg attcaagggc tgatcctaag aagaagagga
     7321 aggtttgact cgagatatga agatgaagat gaaatatttg gtgtgtcaaa taaaaagctt
     7381 gtgtgcttaa gtttgtgttt ttttcttggc ttgttgtgtt atgaatttgt ggctttttct
     7441 aatattaaat gaatgtaaga tcacattata atgaataaac aaatgtttct ataatccatt
     7501 gtgaatgttt tgttggatct cttctgcagc atataactac tgtatgtgct atggtatgga
     7561 ctatggaata tgattaaaga taaggagctc aaactatcag tgtttgacag gatatattgg
     7621 cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga
     7681 aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat
     7741 caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg
     7801 cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc
     7861 ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc
     7921 gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat
     7981 gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc
     8041 ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg
     8101 gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc
     8161 ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg
     8221 ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg
     8281 ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg
     8341 agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc
     8401 ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag
     8461 gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag
     8521 gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag
     8581 gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc
     8641 aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg
     8701 ccgggtacgt gttcgagccg cccgcgcacg gctcaaccgt gcggctgcat gaaatcctgg
     8761 ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg
     8821 agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg
     8881 ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat
     8941 cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg
     9001 cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc
     9061 ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc
     9121 gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc
     9181 gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc
     9241 ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca
     9301 gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa
     9361 aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct
     9421 tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt
     9481 tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat
     9541 taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg
     9601 ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca
     9661 gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg
     9721 aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg
     9781 cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg
     9841 aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg
     9901 aggaacgggc ggttggccag gcgtaagcgg ctgggttgcc tgccggccct gcaatggcac
     9961 tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa
    10021 atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag
    10081 cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga
    10141 atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc
    10201 aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat
    10261 agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc
    10321 gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc
    10381 atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc
    10441 atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt
    10501 gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta
    10561 gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag
    10621 aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta
    10681 aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc
    10741 gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc
    10801 gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa
    10861 gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag
    10921 ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag
    10981 gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc
    11041 gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg
    11101 gaaaaaggtc gaaaaggcct ctttcctgtg gatagcacgt acattgggaa cccaaagccg
    11161 tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca
    11221 cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta
    11281 aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca
    11341 gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct
    11401 tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat
    11461 ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc
    11521 accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    11581 aacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcgcgtc agcgggtgtt
    11641 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
    11701 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
    11761 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
    11821 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    11881 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    11941 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    12001 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    12061 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    12121 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    12181 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    12241 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    12301 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    12361 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    12421 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    12481 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    12541 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    12601 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct aggtactaaa
    12661 acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta
    12721 agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc
    12781 agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac
    12841 ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa
    12901 gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg
    12961 gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat
    13021 ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg
    13081 agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat
    13141 cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc
    13201 catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca
    13261 tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt
    13321 ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt
    13381 ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca
    13441 ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc
    13501 taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca
    13561 gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc
    13621 gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc
    13681 taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat
    13741 agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc
    13801 cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc
    13861 tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt
    13921 aataacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattaat tcgggggatc
    13981 tggattttag tactggattt tggttttagg aattagaaat tttattgata gaagtatttt
    14041 acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata
    14101 ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa aatagagctt
    14161 gtcgatcgtt aattaatcac ctccaagatc ccggacgagt gctggggcgt cggtttccac
    14221 tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt
    14281 gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc
    14341 aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc
    14401 ggagcatata cgcccggagc cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt
    14461 agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct
    14521 cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat
    14581 tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc
    14641 agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt
    14701 cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac
    14761 gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc
    14821 taagatcggc cgcagcgatc gcatccatgg cctccgcgac cggctgcaga acagcgggca
    14881 gttcggtttc aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg
    14941 tcaggctctc gctgaattcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg
    15001 caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca
    15061 ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga
    15121 gctgcatcag gtcggacacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg
    15181 cggtgagttc aggctttttc atcctaggag agatagattt gtagagagag actggtgatt
    15241 tcagcgtgtc ctctccaaat gaaatgaact tccttatata gaggaagggt cttgcgaagg
    15301 atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat ccacttgctt
    15361 tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc
    15421 tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat cgcaatgatg
    15481 gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg
    15541 gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct
    15601 ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac
    15661 catgttcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga
    15721 tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga
    15781 tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc
    15841 ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc
    15901 tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg
    15961 agtagacgag agtgtcgtgc tccaccatg
//
