LOCUS       sequence-272836-       14957 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iSTU-CRISPR/Cas9 with OsCDPK2intron, RZ structure backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14957)
  AUTHORS   Zhong Z, Liu S, Liu X, Liu B, Tang X, Ren Q, Zhou J, Zheng X, Qi Y, 
            Zhang Y
  TITLE     Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome 
            Editing.
  JOURNAL   Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.1186/s12284-020-0369-8.
  PUBMED    32016614
REFERENCE   2  (bases 1 to 14957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Rice (N 
            Y)."; date: "2020-02-3"; volume: "13(1):8. doi"; pages: " 
            10.1186/s12284-020-0369-8"
COMMENT     Sequence Label: pGEL082
FEATURES             Location/Qualifiers
     source          1..14957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2004
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     misc_RNA        3521..3596
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             4201..6585
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(C)
                     /translation="CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILED
                     IVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGK
                     TILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKG
                     ILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQI
                     LKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDN
                     KVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDK
                     AGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMP
                     QVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKV
                     EKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELEN
                     GRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKY
                     FDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     CDS             6598..6618
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6629..6877
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    6898..6922
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8223..8852
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9281..10354
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10420..10614
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10850..10872)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10948..11088
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11003..11022
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11103..11120)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11274..11862)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11354..11373)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11949..12743)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    13168..13192
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    13270..13444
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(14540..14885)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(14577..14597)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa ggtgagcatt
       61 gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca
      121 gtttatctat ctttatacat atatttaaac tttactctac gaataatata atctatagta
      181 ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag
      241 gacaattgag tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt
      301 ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt attagtacat
      361 ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac atctatttta
      421 ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata
      481 atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga
      541 aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa
      601 cgccgtcgac gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag
      661 cgaagcagac ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc
      721 caccgttgga cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg
      781 agccggcacg gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc
      841 tttcccaccg ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc
      901 acaccctctt tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagaactccc
      961 ccaaatccac ccgtcggcac ctccgcttca aggtacgccg ctcgtcctcc ccccccccct
     1021 ctctaccttc tcaagatcgg cgttccggtc catggttagg gcccggtagt tctacttctg
     1081 ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg ctactagcgt tcgtacacgg
     1141 atgcgacctg tacgtcagac acgttctgat tgctaacttg ccagtgtttc tctttgggga
     1201 atcctgggat ggctctagcc gttccgcaga cgggatcgat ttcatgattt tttttgtttc
     1261 gttgcatagg gtttggtttg cccttttcct ttatttcaat atatgccgtg cacttgtttg
     1321 tcgggtcatc ttttcatgct tttttttgtc ttggttgtga tgatgtggtc tggttgggcg
     1381 gtcgttcaag atcggagtag aattaattct gtttcaaact acctggtgga tttattaatt
     1441 ttggatctgt atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga
     1501 aatatcgatc taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga
     1561 tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttca
     1621 agatcggagt agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat
     1681 gtgtgtgtca tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga
     1741 taggtataca tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc
     1801 tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat
     1861 tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt ggattttttt
     1921 agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc
     1981 tgttgtttgg tgttacttct gcagcctgca ggtagatcgc tcgtcgacat ggataagaag
     2041 tactctatcg gactcgatat cggaactaac tctgtgggat gggctgtgat caccgatgag
     2101 tacaaggtgc catctaagaa gttcaaggtt ctcggaaaca ccgataggca ctctatcaag
     2161 aaaaacctta tcggtgctct cctcttcgat tctggtgaaa ctgctgaggc taccagactc
     2221 aagagaaccg ctagaagaag gtacaccaga agaaagaaca ggatctgcta cctccaagag
     2281 atcttctcta acgagatggc taaagtggat gattcattct tccacaggct cgaagagtca
     2341 ttcctcgtgg aagaagataa gaagcacgag aggcacccta tcttcggaaa catcgttgat
     2401 gaggtggcat accacgagaa gtaccctact atctaccacc tcagaaagaa gctcgttgat
     2461 tctactgata aggctgatct caggctcatc tacctcgctc tcgctcacat gatcaagttc
     2521 agaggacact tcctcatcga gggtgatctc aaccctgata actctgatgt ggataagttg
     2581 ttcatccagc tcgtgcagac ctacaaccag cttttcgaag agaaccctat caacgcttca
     2641 ggtgtggatg ctaaggctat cctctctgct aggctctcta agtcaagaag gcttgagaac
     2701 ctcattgctc agctccctgg tgagaagaag aacggacttt tcggaaactt gatcgctctc
     2761 tctctcggac tcacccctaa cttcaagtct aacttcgatc tcgctgagga tgcaaagctc
     2821 cagctctcaa aggataccta cgatgatgat ctcgataacc tcctcgctca gatcggagat
     2881 cagtacgctg atttgttcct cgctgctaag aacctctctg atgctatcct cctcagtgat
     2941 atcctcagag tgaacaccga gatcaccaag gctccactct cagcttctat gatcaagaga
     3001 tacgatgagc accaccagga tctcacactt ctcaaggctc ttgttagaca gcagctccca
     3061 gagaagtaca aagagatttt cttcgatcag tctaagaacg gatacgctgg ttacatcgat
     3121 ggtggtgcat ctcaagaaga gttctacaag ttcatcaagc ctatcctcga gaagatggat
     3181 ggaaccgagg aactcctcgt gaagctcaat agagaggatc ttctcagaaa gcagaggacc
     3241 ttcgataacg gatctatccc tcatcagatc cacctcggag agttgcacgc tatccttaga
     3301 aggcaagagg atttctaccc attcctcaag gataacaggg aaaagattga gaagattctc
     3361 accttcagaa tcccttacta cgtgggacct ctcgctagag gaaactcaag attcgcttgg
     3421 atgaccagaa agtctgagga aaccatcacc ccttggaact tcgaagaggt caatatgctg
     3481 tcaaaggcag cttttgtcac cggaggagac cttggtctca gttttagagc tagaaatagc
     3541 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
     3601 gtcaccggat gtgttttccg gtctgatgag tccgtgagga cgaaacaaaa gctttctttt
     3661 cttttccttt tcgttgcttg ttttcccctt ctgatgttgt gtgttgagtt tattgtttgg
     3721 tttgctggta gatccagtat ttggatcatt tttccttttt tttttctttc attgcttctg
     3781 cggagggatg agtttgtgca tgaatctttt gttggttgga ttcgcttagt tttcacacta
     3841 gtcctttgat tcagcgcaaa tctttgttaa ttggctcgct taattctgac ataaagtgtt
     3901 ccttcttggt tctgcttgca ggtggtggat aagggtgcta gtgctcagtc tttcatcgag
     3961 aggatgacca acttcgataa gaaccttcca aacgagaagg tgctccctaa gcactctttg
     4021 ctctacgagt acttcaccgt gtacaacgag ttgaccaagg ttaagtacgt gaccgaggga
     4081 atgaggaagc ctgctttttt gtcaggtgag caaaagaagg ctatcgttga tctcttgttc
     4141 aagaccaaca gaaaggtgac cgtgaagcag ctcaaagagg attacttcaa gaaaatcgag
     4201 tgcttcgatt cagttgagat ttctggtgtt gaggataggt tcaacgcatc tctcggaacc
     4261 taccacgatc tcctcaagat cattaaggat aaggatttct tggataacga ggaaaacgag
     4321 gatatcttgg aggatatcgt tcttaccctc accctctttg aagatagaga gatgattgaa
     4381 gaaaggctca agacctacgc tcatctcttc gatgataagg tgatgaagca gttgaagaga
     4441 agaagataca ctggttgggg aaggctctca agaaagctca ttaacggaat cagggataag
     4501 cagtctggaa agacaatcct tgatttcctc aagtctgatg gattcgctaa cagaaacttc
     4561 atgcagctca tccacgatga ttctctcacc tttaaagagg atatccagaa ggctcaggtt
     4621 tcaggacagg gtgatagtct ccatgagcat atcgctaacc tcgctggatc tcctgcaatc
     4681 aagaagggaa tcctccagac tgtgaaggtt gtggatgagt tggtgaaggt gatgggaagg
     4741 cataagcctg agaacatcgt gatcgaaatg gctagagaga accagaccac tcagaaggga
     4801 cagaagaact ctagggaaag gatgaagagg atcgaggaag gtatcaaaga gcttggatct
     4861 cagatcctca aagagcaccc tgttgagaac actcagctcc agaatgagaa gctctacctc
     4921 tactacctcc agaacggaag ggatatgtat gtggatcaag agttggatat caacaggctc
     4981 tctgattacg atgttgatca tatcgtgcca cagtcattct tgaaggatga ttctatcgat
     5041 aacaaggtgc tcaccaggtc tgataagaac aggggtaaga gtgataacgt gccaagtgaa
     5101 gaggttgtga agaaaatgaa gaactattgg aggcagctcc tcaacgctaa gctcatcact
     5161 cagagaaagt tcgataactt gactaaggct gagaggggag gactctctga attggataag
     5221 gcaggattca tcaagaggca gcttgtggaa accaggcaga tcactaagca cgttgcacag
     5281 atcctcgatt ctaggatgaa caccaagtac gatgagaacg ataagttgat cagggaagtg
     5341 aaggttatca ccctcaagtc aaagctcgtg tctgatttca gaaaggattt ccaattctac
     5401 aaggtgaggg aaatcaacaa ctaccaccac gctcacgatg cttaccttaa cgctgttgtt
     5461 ggaaccgctc tcatcaagaa gtatcctaag ctcgagtcag agttcgtgta cggtgattac
     5521 aaggtgtacg atgtgaggaa gatgatcgct aagtctgagc aagagatcgg aaaggctacc
     5581 gctaagtatt tcttctactc taacatcatg aatttcttca agaccgagat taccctcgct
     5641 aacggtgaga tcagaaagag gccactcatc gagacaaacg gtgaaacagg tgagatcgtg
     5701 tgggataagg gaagggattt cgctaccgtt agaaaggtgc tctctatgcc acaggtgaac
     5761 atcgttaaga aaaccgaggt gcagaccggt ggattctcta aagagtctat cctccctaag
     5821 aggaactctg ataagctcat tgctaggaag aaggattggg accctaagaa atacggtggt
     5881 ttcgattctc ctaccgtggc ttactctgtt ctcgttgtgg ctaaggttga gaagggaaag
     5941 agtaagaagc tcaagtctgt taaggaactt ctcggaatca ctatcatgga aaggtcatct
     6001 ttcgagaaga acccaatcga tttcctcgag gctaagggat acaaagaggt taagaaggat
     6061 ctcatcatca agctcccaaa gtactcactc ttcgaactcg agaacggtag aaagaggatg
     6121 ctcgcttctg ctggtgagct tcaaaaggga aacgagcttg ctctcccatc taagtacgtt
     6181 aactttcttt acctcgcttc tcactacgag aagttgaagg gatctccaga agataacgag
     6241 cagaagcaac ttttcgttga gcagcacaag cactacttgg atgagatcat cgagcagatc
     6301 tctgagttct ctaaaagggt gatcctcgct gatgcaaacc tcgataaggt gttgtctgct
     6361 tacaacaagc acagagataa gcctatcagg gaacaggcag agaacatcat ccatctcttc
     6421 acccttacca acctcggtgc tcctgctgct ttcaagtact tcgatacaac catcgatagg
     6481 aagagataca cctctaccaa agaagtgctc gatgctaccc tcatccatca gtctatcact
     6541 ggactctacg agactaggat cgatctctca cagctcggtg gtgattcaag ggctgatcct
     6601 aagaagaaga ggaaggtttg actcgagata tgaagatgaa gatgaaatat ttggtgtgtc
     6661 aaataaaaag cttgtgtgct taagtttgtg tttttttctt ggcttgttgt gttatgaatt
     6721 tgtggctttt tctaatatta aatgaatgta agatcacatt ataatgaata aacaaatgtt
     6781 tctataatcc attgtgaatg ttttgttgga tctcttctgc agcatataac tactgtatgt
     6841 gctatggtat ggactatgga atatgattaa agataaggag ctcaaactat cagtgtttga
     6901 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt
     6961 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg
     7021 ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt
     7081 ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg
     7141 acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa
     7201 gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg
     7261 cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga
     7321 actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga
     7381 ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac
     7441 caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca
     7501 ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc
     7561 cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat
     7621 cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg
     7681 ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg
     7741 caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact
     7801 tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga
     7861 cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc
     7921 atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg
     7981 gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acggctcaac cgtgcggctg
     8041 catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc
     8101 gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg
     8161 cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac
     8221 gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa
     8281 cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc
     8341 cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg
     8401 gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag
     8461 tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact
     8521 tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg
     8581 agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt
     8641 cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg
     8701 agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg
     8761 ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc
     8821 tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa
     8881 aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt
     8941 ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga
     9001 tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc
     9061 tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt
     9121 tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat
     9181 gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt gcctgccggc
     9241 cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc
     9301 cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc
     9361 gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc
     9421 ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat
     9481 taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt
     9541 gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga
     9601 ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc
     9661 agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca
     9721 tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt
     9781 ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa
     9841 agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac
     9901 gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg
     9961 ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga
    10021 ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga
    10081 ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc
    10141 aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg
    10201 agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga
    10261 gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa
    10321 cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat
    10381 tgccctagca ggggaaaaag gtcgaaaagg cctctttcct gtggatagca cgtacattgg
    10441 gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat
    10501 tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc
    10561 ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc
    10621 tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct
    10681 acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct
    10741 acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg
    10801 cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
    10861 tgcagctccc ggaaacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagcgc
    10921 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
    10981 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
    11041 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
    11101 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    11161 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    11221 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    11281 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    11341 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    11401 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    11461 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    11521 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    11581 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    11641 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    11701 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    11761 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    11821 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    11881 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat
    11941 tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc
    12001 ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg
    12061 atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga
    12121 tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg
    12181 tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc
    12241 ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta
    12301 ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc
    12361 gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca
    12421 gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc
    12481 agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct
    12541 tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg
    12601 ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata taccttagca
    12661 ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc
    12721 ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga
    12781 taccccaaga agctaattat aacaagacga actccaattc actgttcctt gcattctaaa
    12841 accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag
    12901 tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt
    12961 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
    13021 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
    13081 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
    13141 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac
    13201 gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt
    13261 aattcggggg atctggattt tagtactgga ttttggtttt aggaattaga aattttattg
    13321 atagaagtat tttacaaata caaatacata ctaagggttt cttatatgct caacacatga
    13381 gcgaaaccct ataggaaccc taattccctt atctgggaac tactcacaca ttattatgga
    13441 gaaaatagag cttgtcgatc gttaattaat cacctccaag atcccggacg agtgctgggg
    13501 cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt
    13561 ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat
    13621 cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg
    13681 tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag ctccggatgc
    13741 ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag
    13801 atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct
    13861 gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc
    13921 cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac
    13981 gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg
    14041 catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac
    14101 ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tggcctccgc gaccggctgc
    14161 agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg
    14221 gagatgcaat aggtcaggct ctcgctgaat tccccaatgt caagcacttc cggaatcggg
    14281 agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag
    14341 ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct
    14401 tcgccctccg agagctgcat caggtcggac acgctgtcga acttttcgat cagaaacttc
    14461 tcgacagacg tcgcggtgag ttcaggcttt ttcatcctag gagagataga tttgtagaga
    14521 gagactggtg atttcagcgt gtcctctcca aatgaaatga acttccttat atagaggaag
    14581 ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc
    14641 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg
    14701 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt
    14761 tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt
    14821 gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag
    14881 tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag tagacgagag
    14941 tgtcgtgctc caccatg
//
