LOCUS       sequence-272844-       15890 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iSTU-CRISPR/Cas9 with RcCat intron, tRNA structure backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15890)
  AUTHORS   Zhong Z, Liu S, Liu X, Liu B, Tang X, Ren Q, Zhou J, Zheng X, Qi Y, 
            Zhang Y
  TITLE     Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome 
            Editing.
  JOURNAL   Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.1186/s12284-020-0369-8.
  PUBMED    32016614
REFERENCE   2  (bases 1 to 15890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Rice (N 
            Y)."; date: "2020-02-3"; volume: "13(1):8. doi"; pages: " 
            10.1186/s12284-020-0369-8"
COMMENT     Sequence Label: pGEL085
FEATURES             Location/Qualifiers
     source          1..15890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2006
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             complement(3588..3893)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMITTDMASVPVSVIVEEVADLSHRENDIKNAINLMFWGI"
     misc_RNA        4220..4295
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             4809..7193
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(C)
                     /translation="CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILED
                     IVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGK
                     TILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKG
                     ILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQI
                     LKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDN
                     KVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDK
                     AGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMP
                     QVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKV
                     EKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELEN
                     GRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKY
                     FDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     CDS             7206..7226
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7237..7485
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    7506..7530
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8831..9460
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9889..10962
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11028..11222
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11458..11480)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11556..11696
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11611..11630
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11711..11728)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11882..12470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11962..11981)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12557..13351)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    13776..13800
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    13878..14052
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(15142..15818)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(15185..15205)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa ggtgagcatt
       61 gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca
      121 gtttatctat ctttatacat atatttaaac tttactctac gaataatata atctatagta
      181 ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag
      241 gacaattgag tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt
      301 ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt attagtacat
      361 ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac atctatttta
      421 ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata
      481 atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga
      541 aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa
      601 cgccgtcgac gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag
      661 cgaagcagac ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc
      721 caccgttgga cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg
      781 agccggcacg gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc
      841 tttcccaccg ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc
      901 acaccctctt tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagaactccc
      961 ccaaatccac ccgtcggcac ctccgcttca aggtacgccg ctcgtcctcc cccccccccc
     1021 ctctctacct tctcaagatc ggcgttccgg tccatggtta gggcccggta gttctacttc
     1081 tgttcatgtt tgtgttagat ccgtgtttgt gttagatccg tgctactagc gttcgtacac
     1141 ggatgcgacc tgtacgtcag acacgttctg attgctaact tgccagtgtt tctctttggg
     1201 gaatcctggg atggctctag ccgttccgca gacgggatcg atttcatgat tttttttgtt
     1261 tcgttgcata gggtttggtt tgcccttttc ctttatttca atatatgccg tgcacttgtt
     1321 tgtcgggtca tcttttcatg cttttttttg tcttggttgt gatgatgtgg tctggttggg
     1381 cggtcgttca agatcggagt agaattaatt ctgtttcaaa ctacctggtg gatttattaa
     1441 ttttggatct gtatgtgtgt gccatacata ttcatagtta cgaattgaag atgatggatg
     1501 gaaatatcga tctaggatag gtatacatgt tgatgcgggt tttactgatg catatacaga
     1561 gatgcttttt gttcgcttgg ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt
     1621 caagatcgga gtagaatact gtttcaaact acctggtgta tttattaatt ttggaactgt
     1681 atgtgtgtgt catacatctt catagttacg agtttaagat ggatggaaat atcgatctag
     1741 gataggtata catgttgatg tgggttttac tgatgcatat acatgatggc atatgcagca
     1801 tctattcata tgctctaacc ttgagtacct atctattata ataaacaagt atgttttata
     1861 attattttga tcttgatata cttggatgat ggcatatgca gcagctatat gtggattttt
     1921 ttagccctgc cttcatacgc tatttatttg cttggtactg tttcttttgt cgatgctcac
     1981 cctgttgttt ggtgttactt ctgcagcctg caggtagatc gctcgtcgac atggataaga
     2041 agtactctat cggactcgat atcggaacta actctgtggg atgggctgtg atcaccgatg
     2101 agtacaaggt gccatctaag aagttcaagg ttctcggaaa caccgatagg cactctatca
     2161 agaaaaacct tatcggtgct ctcctcttcg attctggtga aactgctgag gctaccagac
     2221 tcaagagaac cgctagaaga aggtacacca gaagaaagaa caggatctgc tacctccaag
     2281 agatcttctc taacgagatg gctaaagtgg atgattcatt cttccacagg ctcgaagagt
     2341 cattcctcgt ggaagaagat aagaagcacg agaggcaccc tatcttcgga aacatcgttg
     2401 atgaggtggc ataccacgag aagtacccta ctatctacca cctcagaaag aagctcgttg
     2461 attctactga taaggctgat ctcaggctca tctacctcgc tctcgctcac atgatcaagt
     2521 tcagaggaca cttcctcatc gagggtgatc tcaaccctga taactctgat gtggataagt
     2581 tgttcatcca gctcgtgcag acctacaacc agcttttcga agagaaccct atcaacgctt
     2641 caggtgtgga tgctaaggct atcctctctg ctaggctctc taagtcaaga aggcttgaga
     2701 acctcattgc tcagctccct ggtgagaaga agaacggact tttcggaaac ttgatcgctc
     2761 tctctctcgg actcacccct aacttcaagt ctaacttcga tctcgctgag gatgcaaagc
     2821 tccagctctc aaaggatacc tacgatgatg atctcgataa cctcctcgct cagatcggag
     2881 atcagtacgc tgatttgttc ctcgctgcta agaacctctc tgatgctatc ctcctcagtg
     2941 atatcctcag agtgaacacc gagatcacca aggctccact ctcagcttct atgatcaaga
     3001 gatacgatga gcaccaccag gatctcacac ttctcaaggc tcttgttaga cagcagctcc
     3061 cagagaagta caaagagatt ttcttcgatc agtctaagaa cggatacgct ggttacatcg
     3121 atggtggtgc atctcaagaa gagttctaca agttcatcaa gcctatcctc gagaagatgg
     3181 atggaaccga ggaactcctc gtgaagctca atagagagga tcttctcaga aagcagagga
     3241 ccttcgataa cggatctatc cctcatcaga tccacctcgg agagttgcac gctatcctta
     3301 gaaggcaaga ggatttctac ccattcctca aggataacag ggaaaagatt gagaagattc
     3361 tcaccttcag aatcccttac tacgtgggac ctctcgctag aggaaactca agattcgctt
     3421 ggatgaccag aaagtctgag gaaaccatca ccccttggaa cttcgaagag gtaaatttct
     3481 agtttttctc cttcattttc ttgaacaaag caccagtggt ctagtggtag aatagtaccc
     3541 tgccacggta cagacccggg ttcgattccc ggctggtgca ggagacctta tattccccag
     3601 aacatcaggt taatggcgtt tttgatgtca ttttcgcggt ggctgagatc agccacttct
     3661 tccacgataa cggaaaccgg cacactggcc atatcggtgg taatcatgcg ccagctttca
     3721 tccccgatat gcaccaccgg gtaaagttca cgggagactt tatctgacag cagacgtgca
     3781 ctggccaggg ggatcaccat ccgtcgcccg ggcgtgtcaa taatatcact ctgtacatcc
     3841 acaaacagac gataacggct ctctctttta taggtgtaaa ccttaaactg catttcacca
     3901 gcccctgttc tcgtcagcaa aagagccgtt catttcaata aaccgggcga cctcagccat
     3961 cccttcctga ttttccgctt tccagcgttc ggcacgcaga cgacgggctt cattctgcat
     4021 ggttgtgctt accagaccgg agatattgac atcatatatg ccttgagcaa ctgatagctg
     4081 tcgctgtcaa ctgtcactgt aatacgctgc ttcatagcat acctcttttt gacatacttc
     4141 gggtatacat atcagtatat attcttatac cgcaaaaatc agcgcgcaaa tacgcatact
     4201 gttatctggc ttggtctcag ttttagagct agaaatagca agttaaaata aggctagtcc
     4261 gttatcaact tgaaaaagtg gcaccgagtc ggtgcaacaa agcaccagtg gtctagtggt
     4321 agaatagtac cctgccacgg tacagacccg ggttcgattc ccggctggtg cagttaggac
     4381 ccttttctct ttttattttt ttgagctttg atctttcttt aaactgatct attttttaat
     4441 tgattggtta tggtgtaaat attacatagc tttaactgat aatctgatta ctttatttcg
     4501 tgtgtctatg atgatgatga tagttacagg tggtggataa gggtgctagt gctcagtctt
     4561 tcatcgagag gatgaccaac ttcgataaga accttccaaa cgagaaggtg ctccctaagc
     4621 actctttgct ctacgagtac ttcaccgtgt acaacgagtt gaccaaggtt aagtacgtga
     4681 ccgagggaat gaggaagcct gcttttttgt caggtgagca aaagaaggct atcgttgatc
     4741 tcttgttcaa gaccaacaga aaggtgaccg tgaagcagct caaagaggat tacttcaaga
     4801 aaatcgagtg cttcgattca gttgagattt ctggtgttga ggataggttc aacgcatctc
     4861 tcggaaccta ccacgatctc ctcaagatca ttaaggataa ggatttcttg gataacgagg
     4921 aaaacgagga tatcttggag gatatcgttc ttaccctcac cctctttgaa gatagagaga
     4981 tgattgaaga aaggctcaag acctacgctc atctcttcga tgataaggtg atgaagcagt
     5041 tgaagagaag aagatacact ggttggggaa ggctctcaag aaagctcatt aacggaatca
     5101 gggataagca gtctggaaag acaatccttg atttcctcaa gtctgatgga ttcgctaaca
     5161 gaaacttcat gcagctcatc cacgatgatt ctctcacctt taaagaggat atccagaagg
     5221 ctcaggtttc aggacagggt gatagtctcc atgagcatat cgctaacctc gctggatctc
     5281 ctgcaatcaa gaagggaatc ctccagactg tgaaggttgt ggatgagttg gtgaaggtga
     5341 tgggaaggca taagcctgag aacatcgtga tcgaaatggc tagagagaac cagaccactc
     5401 agaagggaca gaagaactct agggaaagga tgaagaggat cgaggaaggt atcaaagagc
     5461 ttggatctca gatcctcaaa gagcaccctg ttgagaacac tcagctccag aatgagaagc
     5521 tctacctcta ctacctccag aacggaaggg atatgtatgt ggatcaagag ttggatatca
     5581 acaggctctc tgattacgat gttgatcata tcgtgccaca gtcattcttg aaggatgatt
     5641 ctatcgataa caaggtgctc accaggtctg ataagaacag gggtaagagt gataacgtgc
     5701 caagtgaaga ggttgtgaag aaaatgaaga actattggag gcagctcctc aacgctaagc
     5761 tcatcactca gagaaagttc gataacttga ctaaggctga gaggggagga ctctctgaat
     5821 tggataaggc aggattcatc aagaggcagc ttgtggaaac caggcagatc actaagcacg
     5881 ttgcacagat cctcgattct aggatgaaca ccaagtacga tgagaacgat aagttgatca
     5941 gggaagtgaa ggttatcacc ctcaagtcaa agctcgtgtc tgatttcaga aaggatttcc
     6001 aattctacaa ggtgagggaa atcaacaact accaccacgc tcacgatgct taccttaacg
     6061 ctgttgttgg aaccgctctc atcaagaagt atcctaagct cgagtcagag ttcgtgtacg
     6121 gtgattacaa ggtgtacgat gtgaggaaga tgatcgctaa gtctgagcaa gagatcggaa
     6181 aggctaccgc taagtatttc ttctactcta acatcatgaa tttcttcaag accgagatta
     6241 ccctcgctaa cggtgagatc agaaagaggc cactcatcga gacaaacggt gaaacaggtg
     6301 agatcgtgtg ggataaggga agggatttcg ctaccgttag aaaggtgctc tctatgccac
     6361 aggtgaacat cgttaagaaa accgaggtgc agaccggtgg attctctaaa gagtctatcc
     6421 tccctaagag gaactctgat aagctcattg ctaggaagaa ggattgggac cctaagaaat
     6481 acggtggttt cgattctcct accgtggctt actctgttct cgttgtggct aaggttgaga
     6541 agggaaagag taagaagctc aagtctgtta aggaacttct cggaatcact atcatggaaa
     6601 ggtcatcttt cgagaagaac ccaatcgatt tcctcgaggc taagggatac aaagaggtta
     6661 agaaggatct catcatcaag ctcccaaagt actcactctt cgaactcgag aacggtagaa
     6721 agaggatgct cgcttctgct ggtgagcttc aaaagggaaa cgagcttgct ctcccatcta
     6781 agtacgttaa ctttctttac ctcgcttctc actacgagaa gttgaaggga tctccagaag
     6841 ataacgagca gaagcaactt ttcgttgagc agcacaagca ctacttggat gagatcatcg
     6901 agcagatctc tgagttctct aaaagggtga tcctcgctga tgcaaacctc gataaggtgt
     6961 tgtctgctta caacaagcac agagataagc ctatcaggga acaggcagag aacatcatcc
     7021 atctcttcac ccttaccaac ctcggtgctc ctgctgcttt caagtacttc gatacaacca
     7081 tcgataggaa gagatacacc tctaccaaag aagtgctcga tgctaccctc atccatcagt
     7141 ctatcactgg actctacgag actaggatcg atctctcaca gctcggtggt gattcaaggg
     7201 ctgatcctaa gaagaagagg aaggtttgac tcgagatatg aagatgaaga tgaaatattt
     7261 ggtgtgtcaa ataaaaagct tgtgtgctta agtttgtgtt tttttcttgg cttgttgtgt
     7321 tatgaatttg tggctttttc taatattaaa tgaatgtaag atcacattat aatgaataaa
     7381 caaatgtttc tataatccat tgtgaatgtt ttgttggatc tcttctgcag catataacta
     7441 ctgtatgtgc tatggtatgg actatggaat atgattaaag ataaggagct caaactatca
     7501 gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat
     7561 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa
     7621 ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca
     7681 gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa
     7741 gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt
     7801 cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc
     7861 gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa
     7921 cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc
     7981 aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg
     8041 acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag
     8101 cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc
     8161 acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc
     8221 ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt
     8281 ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag
     8341 gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac
     8401 cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc
     8461 cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag
     8521 gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga
     8581 tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac ggctcaaccg
     8641 tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca
     8701 gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa
     8761 acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca
     8821 aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac
     8881 catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga
     8941 ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac
     9001 cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga
     9061 cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc
     9121 agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga
     9181 cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt
     9241 tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc
     9301 cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac
     9361 tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt
     9421 ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa
     9481 atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct
     9541 gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg
     9601 gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag
     9661 ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag
     9721 atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc
     9781 cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgc
     9841 ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc
     9901 aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg
     9961 aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg
    10021 tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg
    10081 ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc
    10141 tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg
    10201 aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag
    10261 gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg
    10321 gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc
    10381 cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga
    10441 aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg
    10501 cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg
    10561 attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag
    10621 ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt
    10681 caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc
    10741 cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc
    10801 agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac
    10861 ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc
    10921 taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta
    10981 gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggcc tctttcctgt ggatagcacg
    11041 tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag
    11101 ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat
    11161 ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca
    11221 taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg
    11281 cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg
    11341 gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac
    11401 cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
    11461 ctgacacatg cagctcccgg aaacggtcac agcttgtctg taagcggatg ccgggagcag
    11521 acaagcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
    11581 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
    11641 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
    11701 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
    11761 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
    11821 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    11881 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    11941 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    12001 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    12061 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    12121 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    12181 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    12241 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    12301 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
    12361 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    12421 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    12481 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    12541 tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc
    12601 aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat
    12661 cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc
    12721 tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca
    12781 ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca
    12841 gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca
    12901 gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg
    12961 gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa
    13021 tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac
    13081 tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca
    13141 gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt
    13201 tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata
    13261 ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt
    13321 tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt ttctacagta
    13381 tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc
    13441 attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta
    13501 taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg
    13561 tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc
    13621 ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg
    13681 gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg
    13741 ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac
    13801 aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac
    13861 gccgaattaa ttcgggggat ctggatttta gtactggatt ttggttttag gaattagaaa
    13921 ttttattgat agaagtattt tacaaataca aatacatact aagggtttct tatatgctca
    13981 acacatgagc gaaaccctat aggaacccta attcccttat ctgggaacta ctcacacatt
    14041 attatggaga aaatagagct tgtcgatcgt taattaatca cctccaagat cccggacgag
    14101 tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg
    14161 ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg
    14221 cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat
    14281 agagttggtc aagaccaatg cggagcatat acgcccggag ccgcggcgat cctgcaagct
    14341 ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc
    14401 agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa
    14461 tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca
    14521 cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg
    14581 cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag
    14641 caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg
    14701 ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga
    14761 ccggctgcag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg
    14821 cacggcggga gatgcaatag gtcaggctct cgctgaattc cccaatgtca agcacttccg
    14881 gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat
    14941 cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac
    15001 gagattcttc gccctccgag agctgcatca ggtcggacac gctgtcgaac ttttcgatca
    15061 gaaacttctc gacagacgtc gcggtgagtt caggcttttt catcctagga gagatagatt
    15121 tgtagagaga gactggtgat ttcagcgtgt cctctccaaa tgaaatgaac ttccttatat
    15181 agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt cagtggagat
    15241 atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct
    15301 cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc
    15361 ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt
    15421 gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg
    15481 ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta
    15541 gacgagagtg tcgtgctcca ccatgttcac atcaatccac ttgctttgaa gacgtggttg
    15601 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
    15661 cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc
    15721 caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga
    15781 ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga
    15841 ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg
//
