LOCUS       sequence-272847-       14833 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iSTU-CRISPR/Cas12a DR-DR structure crRNA insertion vector with 
            OsCDPK2 intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14833)
  AUTHORS   Zhong Z, Liu S, Liu X, Liu B, Tang X, Ren Q, Zhou J, Zheng X, Qi Y, 
            Zhang Y
  TITLE     Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome 
            Editing.
  JOURNAL   Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.1186/s12284-020-0369-8.
  PUBMED    32016614
REFERENCE   2  (bases 1 to 14833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Rice (N 
            Y)."; date: "2020-02-3"; volume: "13(1):8. doi"; pages: " 
            10.1186/s12284-020-0369-8"
COMMENT     Sequence Label: pGEL088
FEATURES             Location/Qualifiers
     source          1..14833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        37..2035
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             2050..2070
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6122..6169
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      6180..6428
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    6449..6473
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7774..8403
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8832..9905
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9971..10165
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10401..10423)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10499..10639
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10554..10573
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10654..10671)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10825..11413)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10905..10924)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11500..12294)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12719..12743
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    12821..12995
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(14085..14761)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(14128..14148)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ggactagtag tcgtcagtca gtactagccg gcgcgcctgc agtgcagcgt gacccggtcg
       61 tgcccctctc ttgagataag gtgagcattg catgtctaag ttataaaaaa ttaccacata
      121 ttttttttgt cacacttgtt tgaagtgcag tttatctatc tttatacata tatttaaact
      181 ttactctacg aataatataa tctatagtac tacaataata tcagtgtttt agagaatcat
      241 ataaatgaac agttagacat ggtctaaagg acaattgagt attttgacaa caggactcta
      301 cagttttatc tttttagtgt gcatgtgttc tccttttttt ttgcaaatag cttcacctat
      361 ataatacttc atccatttta ttagtacatc catttagggt ttagggttaa tggtttttat
      421 agactaattt ttttagtaca tctattttat tctattttag cctctaaatt aagaaaacta
      481 aaactctatt ttagtttttt tatttaataa tttagatata aaatagaata aaataaagtg
      541 actaaaaatt aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac atttttcttg
      601 tttcgagtag ataatgccag cctgttaaac gccgtcgacg agtctaacgg acaccaacca
      661 gcgaaccagc agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc tctgtcgctg
      721 cctctggacc cctctcgaga gttccgctcc accgttggac ttgctccgct gtcggcatcc
      781 agaaattgcg tggcggagcg gcagacgtga gccggcacgg caggcggcct cctcctcctc
      841 tcacggcacc ggcagctacg ggggattcct ttcccaccgc tccttcgctt tcccttcctc
      901 gcccgccgta ataaatagac accccctcca caccctcttt ccccaacctc gtgttgttcg
      961 gagcgcacac acacacaacc agaactcccc caaatccacc cgtcggcacc tccgcttcaa
     1021 ggtacgccgc tcgtcctccc cccccccccc tctctacctt ctcaagatcg gcgttccggt
     1081 ccatggttag ggcccggtag ttctacttct gttcatgttt gtgttagatc cgtgtttgtg
     1141 ttagatccgt gctactagcg ttcgtacacg gatgcgacct gtacgtcaga cacgttctga
     1201 ttgctaactt gccagtgttt ctctttgggg aatcctggga tggctctagc cgttccgcag
     1261 acgggatcga tttcatgatt ttttttgttt cgttgcatag ggtttggttt gcccttttcc
     1321 tttatttcaa tatatgccgt gcacttgttt gtcgggtcat cttttcatgc ttttttttgt
     1381 cttggttgtg atgatgtggt ctggttgggc ggtcgttcaa gatcggagta gaattaattc
     1441 tgtttcaaac tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat
     1501 tcatagttac gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt
     1561 gatgcgggtt ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg
     1621 tggtgtggtt gggcggtcgt tcattcgttc aagatcggag tagaatactg tttcaaacta
     1681 cctggtgtat ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga
     1741 gtttaagatg gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact
     1801 gatgcatata catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta
     1861 tctattataa taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg
     1921 gcatatgcag cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc
     1981 ttggtactgt ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcctgc
     2041 aggatggctc ctaagaagaa gcggaaggtt ggtattcacg gggtgcctgc ggcttcaaag
     2101 ctcgagaaat tcaccaactg ttattcgttg agcaaaacac tgcggtttaa agcgattcca
     2161 gtcggcaaga ctcaagagaa tatagacaat aagcggctgt tggtggaaga tgaaaagcgc
     2221 gcggaagact acaaaggggt gaagaagttg ttggacagat actacctctc ttttatcaat
     2281 gatgtcttgc actcaatcaa attgaagaat ctgaacaact acatctccct cttcagaaag
     2341 aaaacaagga cagaaaagga gaataaggaa cttgaaaatt tggagatcaa tctgaggaaa
     2401 gagatcgcga aagcctttaa aggcaacgaa ggatacaaaa gtctgttcaa gaaggatata
     2461 attgagacaa ttttgccaga gttcctcgat gacaaggacg agattgcgct ggtcaattcg
     2521 ttcaacggat tcacaacagc attcacaggc ttctttgata atcgggaaaa tatgttctct
     2581 gaggaggcaa agtccacttc tattgcgttc aggtgtatca atgagaatct cactaggtac
     2641 atttccaaca tggatatctt tgagaaggtt gacgcaattt ttgacaagca cgaagttcag
     2701 gagattaagg agaagatcct caattccgat tatgacgttg aggacttctt cgaaggtgag
     2761 ttttttaatt tcgtgctcac tcaagagggt atcgacgtgt ataatgcgat catcggtggg
     2821 ttcgtgactg agtccggtga aaagattaag ggattgaacg agtatatcaa cctttacaac
     2881 caaaagacga aacagaagct gccaaagttc aagcctcttt acaaacaggt tctttcagac
     2941 cgcgagtcac tctcgttcta tggggagggc tacacttcgg atgaggaagt cctggaggtg
     3001 ttcaggaata ctctcaataa gaattcggag attttctctt ctataaaaaa actggaaaag
     3061 ttgtttaaga attttgacga atactctagc gccggcatat ttgtgaaaaa cggcccggcc
     3121 atatcaacga taagtaaaga tatcttcggc gaatggaacg tgatcagaga caaatggaac
     3181 gcggagtatg acgatattca cctgaagaag aaggctgtcg taacggagaa gtacgaggat
     3241 gatcgcagga aaagcttcaa aaagatcgga agtttcagcc tggaacagtt gcaggagtat
     3301 gctgacgccg atcttagcgt cgtcgagaag ttgaaggaga taatcatcca aaaggtcgat
     3361 gagatatata aagtctatgg atcaagtgaa aaactgttcg acgccgactt cgttttggag
     3421 aagtccctga agaagaacga cgctgttgtt gccattatga aggatctgct cgacagcgtg
     3481 aagagtttcg agaactatat taaggctttt ttcggggagg ggaaggagac taacagagat
     3541 gagtccttct acggagactt cgtcctcgcg tacgatatac tccttaaggt agaccacatc
     3601 tacgacgcaa tcagaaatta cgtgacacaa aagccgtaca gcaaggacaa gttcaaactc
     3661 tacttccaga acccccagtt catgggcggc tgggacaagg acaaggaaac ggattacagg
     3721 gctacgatcc tgaggtatgg ttcaaaatac tacttggcga ttatggacaa gaagtacgcc
     3781 aagtgtctcc agaagattga caaagacgat gtcaatggca attatgagaa gatcaactac
     3841 aagctgcttc cgggtccgaa caagatgctc ccaaaggttt tcttcagcaa gaaatggatg
     3901 gcctactata acccaagcga ggacatccag aagatttata agaacggtac gttcaagaag
     3961 ggcgacatgt tcaatcttaa cgactgtcac aagctgatcg acttcttcaa agactcaatt
     4021 agccggtacc caaagtggtc taacgcctat gacttcaact tttcggaaac cgagaagtac
     4081 aaggatatag ccggatttta tagagaggtg gaagagcagg gctacaaggt gtcattcgag
     4141 tccgccagca agaaggaagt ggacaagctc gtggaagagg gtaagctcta catgttccag
     4201 atttataata aagactttag cgataagagc cacgggacac ctaatctcca cacaatgtat
     4261 ttcaagctgc tcttcgacga gaataaccac ggccaaatca ggttgtcagg aggggctgaa
     4321 ctcttcatgc ggcgcgcatc cctgaagaag gaggagcttg tagtccaccc tgcgaatagt
     4381 ccaattgcga ataagaaccc ggacaatcct aaaaagacta caacattgag ctacgacgtg
     4441 tacaaggata agaggttttc cgaggatcag tacgagctcc acatcccgat tgcgatcaac
     4501 aagtgcccaa agaatatttt caagataaac acagaggtgc gtgtactcct gaagcatgac
     4561 gacaatcctt acgtcattgg gattgatcgg ggcgagagga acctcctcta tattgtggtg
     4621 gtggacggga aggggaacat agtcgaacag tactccctta acgaaataat taacaatttc
     4681 aacggcatcc gtatcaagac cgactaccat tcgttgctgg acaagaagga gaaggagaga
     4741 tttgaggcgc ggcaaaattg gacaagtatc gagaacatca aggaactcaa agcaggttat
     4801 atctctcaag ttgtgcataa gatatgcgag ctggttgaga agtatgacgc agtgatcgct
     4861 cttgaggacc tcaactcggg ctttaagaat agccgcgtta aagtggagaa gcaggtctat
     4921 caaaagttcg agaagatgct tatagataag ctcaactaca tggtcgataa gaaatcgaac
     4981 ccatgtgcca ccggcggcgc actcaaaggt taccaaataa caaacaaatt cgagtccttc
     5041 aaatcgatga gtactcagaa tgggttcata ttttatatac cggcgtggct tacgtctaag
     5101 atcgacccgt caactggttt tgtcaacctg ttgaagacga aatacacgtc cattgccgat
     5161 tcgaaaaagt tcatatctag ttttgatcgt attatgtacg tcccagagga agatcttttc
     5221 gagtttgctc tcgactacaa aaacttttcg cggaccgatg cggattacat taaaaaatgg
     5281 aaactctatt cgtacggcaa cagaatcagg atttttcgca accctaagaa gaataacgtc
     5341 tttgattggg aggaagtttg cttgactagc gcgtacaagg tcaatatgct gtcaaaggca
     5401 aatttctact aagtgtagat agagaccggg gtctccaatt tctactaagt gtagattttc
     5461 ttttcttttc cttttcgttg cttgttttcc ccttctgatg ttgtgtgttg agtttattgt
     5521 ttggtttgct ggtagatcca gtatttggat catttttcct tttttttttc tttcattgct
     5581 tctgcggagg gatgagtttg tgcatgaatc ttttgttggt tggattcgct tagttttcac
     5641 actagtcctt tgattcagcg caaatctttg ttaattggct cgcttaattc tgacataaag
     5701 tgttccttct tggttctgct tgcaggagct ctttaataag tatggcatta actaccaaca
     5761 gggtgatatc agagcactgc tttgcgaaca atctgacaag gctttctact catccttcat
     5821 ggctttgatg agcctgatgc tccagatgag aaattcaatt acaggcagaa ccgacgtgga
     5881 tttcttgatc tccccggtta aaaattctga tggcatcttt tacgatagca ggaactatga
     5941 agcgcaagag aatgcgattc tgccaaaaaa tgcagacgcc aacggtgcct ataacatcgc
     6001 caggaaagtc ctgtgggcga tcggccagtt caaaaaggcc gaagacgaaa aattggacaa
     6061 ggtcaaaatc gctatcagca acaaagagtg gctggagtat gctcagacat ccgtaaagca
     6121 taagcgtcct gctgccacca aaaaggccgg acaggctaag aaaaagaagt gatctagaat
     6181 atgaagatga agatgaaata tttggtgtgt caaataaaaa gcttgtgtgc ttaagtttgt
     6241 gtttttttct tggcttgttg tgttatgaat ttgtggcttt ttctaatatt aaatgaatgt
     6301 aagatcacat tataatgaat aaacaaatgt ttctataatc cattgtgaat gttttgttgg
     6361 atctcttctg cagcatataa ctactgtatg tgctatggta tggactatgg aatatgatta
     6421 aagataagga gctcaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag
     6481 aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc
     6541 gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc
     6601 aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa
     6661 acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc
     6721 gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac
     6781 attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg
     6841 acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt
     6901 tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc
     6961 acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc
     7021 gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg
     7081 cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg
     7141 gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg
     7201 ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc
     7261 acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg
     7321 gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg
     7381 aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg
     7441 ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga
     7501 accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga
     7561 gccgcccgcg cacggctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc
     7621 caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa
     7681 aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg
     7741 atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag
     7801 aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc
     7861 ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc
     7921 gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac
     7981 gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac
     8041 ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct
     8101 tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg
     8161 gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc
     8221 ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg
     8281 cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc
     8341 gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt
     8401 tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc
     8461 cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga
     8521 agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac
     8581 gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa
     8641 tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat
     8701 caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg
     8761 ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg gcactggaac ccccaagccc
     8821 gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg
     8881 gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg
     8941 cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc
     9001 ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac
     9061 cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg
     9121 acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg
     9181 agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg
     9241 attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg
     9301 aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt
     9361 tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt
     9421 taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga
     9481 cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc
     9541 ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca
     9601 agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc
     9661 gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca
     9721 agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc
     9781 gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg
     9841 ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt
     9901 cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag
     9961 gcctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga
    10021 acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg
    10081 atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc
    10141 ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa
    10201 aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg
    10261 cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac
    10321 aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt
    10381 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggaaacggt cacagcttgt
    10441 ctgtaagcgg atgccgggag cagacaagcg cgtcagcggg tgttggcggg tgtcggggcg
    10501 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
    10561 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
    10621 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
    10681 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
    10741 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    10801 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    10861 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    10921 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    10981 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    11041 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    11101 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    11161 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    11221 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
    11281 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    11341 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    11401 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    11461 aaactcacgt taagggattt tggtcatgca ttctaggtac taaaacaatt catccagtaa
    11521 aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc
    11581 gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata
    11641 ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt
    11701 cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt
    11761 ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca
    11821 gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg
    11881 gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat
    11941 gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga
    12001 gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa
    12061 gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg
    12121 ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc
    12181 attttctccc accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca
    12241 gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta
    12301 tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg
    12361 aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca
    12421 aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccgcgg
    12481 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
    12541 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
    12601 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
    12661 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
    12721 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
    12781 cgtttttaat gtactgaatt aacgccgaat taattcgggg gatctggatt ttagtactgg
    12841 attttggttt taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat
    12901 actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataggaacc ctaattccct
    12961 tatctgggaa ctactcacac attattatgg agaaaataga gcttgtcgat cgttaattaa
    13021 tcacctccaa gatcccggac gagtgctggg gcgtcggttt ccactatcgg cgagtacttc
    13081 tacacagcca tcggtccaga cggccgcgct tctgcgggcg atttgtgtac gcccgacagt
    13141 cccggctccg gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa
    13201 attgccgtca accaagctct gatagagttg gtcaagacca atgcggagca tatacgcccg
    13261 gagccgcggc gatcctgcaa gctccggatg cctccgctcg aagtagcgcg tctgctgctc
    13321 catacaagcc aaccacggcc tccagaagaa gatgttggcg acctcgtatt gggaatcccc
    13381 gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg ccattgtccg tcaggacatt
    13441 gttggagccg aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct cggcccaaag
    13501 catcagctca tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg
    13561 ccagtgatac acatggggat cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg
    13621 accgattcct tgcggtccga atgggccgaa cccgctcgtc tggctaagat cggccgcagc
    13681 gatcgcatcc atggcctccg cgaccggctg cagaacagcg ggcagttcgg tttcaggcag
    13741 gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc tctcgctgaa
    13801 ttccccaatg tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac
    13861 ataacgatct ttgtagaaac catcggcgca gctatttacc cgcaggacat atccacgccc
    13921 tcctacatcg aagctgaaag cacgagattc ttcgccctcc gagagctgca tcaggtcgga
    13981 cacgctgtcg aacttttcga tcagaaactt ctcgacagac gtcgcggtga gttcaggctt
    14041 tttcatccta ggagagatag atttgtagag agagactggt gatttcagcg tgtcctctcc
    14101 aaatgaaatg aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg
    14161 tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga
    14221 acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg
    14281 gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca
    14341 ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg
    14401 aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact
    14461 gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt cacatcaatc
    14521 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
    14581 gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc
    14641 gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca
    14701 gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc
    14761 aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc
    14821 gtgctccacc atg
//
