LOCUS       sequence-272851-       14924 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iSTU-CRISPR/Cas12a HH-HDV structure crRNA insertion vector with 
            OsCDPK2 intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14924)
  AUTHORS   Zhong Z, Liu S, Liu X, Liu B, Tang X, Ren Q, Zhou J, Zheng X, Qi Y, 
            Zhang Y
  TITLE     Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome 
            Editing.
  JOURNAL   Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.1186/s12284-020-0369-8.
  PUBMED    32016614
REFERENCE   2  (bases 1 to 14924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Rice (N 
            Y)."; date: "2020-02-3"; volume: "13(1):8. doi"; pages: " 
            10.1186/s12284-020-0369-8"
COMMENT     Sequence Label: pGEL089
FEATURES             Location/Qualifiers
     source          1..14924
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        37..2034
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             2049..2069
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     ncRNA           5406..5442
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     ncRNA           5480..5547
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     CDS             6213..6260
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      6271..6519
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    6540..6564
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7865..8494
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8923..9996
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10062..10256
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10492..10514)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10590..10730
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10645..10664
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10745..10762)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10916..11504)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10996..11015)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11591..12385)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12810..12834
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    12912..13086
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(14176..14852)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(14219..14239)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ggactagtag tcgtcagtca gtactagccg gcgcgcctgc agtgcagcgt gacccggtcg
       61 tgcccctctc ttgagataag gtgagcattg catgtctaag ttataaaaaa ttaccacata
      121 ttttttttgt cacacttgtt tgaagtgcag tttatctatc tttatacata tatttaaact
      181 ttactctacg aataatataa tctatagtac tacaataata tcagtgtttt agagaatcat
      241 ataaatgaac agttagacat ggtctaaagg acaattgagt attttgacaa caggactcta
      301 cagttttatc tttttagtgt gcatgtgttc tccttttttt ttgcaaatag cttcacctat
      361 ataatacttc atccatttta ttagtacatc catttagggt ttagggttaa tggtttttat
      421 agactaattt ttttagtaca tctattttat tctattttag cctctaaatt aagaaaacta
      481 aaactctatt ttagtttttt tatttaataa tttagatata aaatagaata aaataaagtg
      541 actaaaaatt aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac atttttcttg
      601 tttcgagtag ataatgccag cctgttaaac gccgtcgacg agtctaacgg acaccaacca
      661 gcgaaccagc agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc tctgtcgctg
      721 cctctggacc cctctcgaga gttccgctcc accgttggac ttgctccgct gtcggcatcc
      781 agaaattgcg tggcggagcg gcagacgtga gccggcacgg caggcggcct cctcctcctc
      841 tcacggcacc ggcagctacg ggggattcct ttcccaccgc tccttcgctt tcccttcctc
      901 gcccgccgta ataaatagac accccctcca caccctcttt ccccaacctc gtgttgttcg
      961 gagcgcacac acacacaacc agaactcccc caaatccacc cgtcggcacc tccgcttcaa
     1021 ggtacgccgc tcgtcctccc ccccccccnt ctctaccttc tcaagatcgg cgttccggtc
     1081 catggttagg gcccggtagt tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt
     1141 tagatccgtg ctactagcgt tcgtacacgg atgcgacctg tacgtcagac acgttctgat
     1201 tgctaacttg ccagtgtttc tctttgggga atcctgggat ggctctagcc gttccgcaga
     1261 cgggatcgat ttcatgattt tttttgtttc gttgcatagg gtttggtttg cccttttcct
     1321 ttatttcaat atatgccgtg cacttgtttg tcgggtcatc ttttcatgct tttttttgtc
     1381 ttggttgtga tgatgtggtc tggttgggcg gtcgttcaag atcggagtag aattaattct
     1441 gtttcaaact acctggtgga tttattaatt ttggatctgt atgtgtgtgc catacatatt
     1501 catagttacg aattgaagat gatggatgga aatatcgatc taggataggt atacatgttg
     1561 atgcgggttt tactgatgca tatacagaga tgctttttgt tcgcttggtt gtgatgatgt
     1621 ggtgtggttg ggcggtcgtt cattcgttca agatcggagt agaatactgt ttcaaactac
     1681 ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca tacatcttca tagttacgag
     1741 tttaagatgg atggaaatat cgatctagga taggtataca tgttgatgtg ggttttactg
     1801 atgcatatac atgatggcat atgcagcatc tattcatatg ctctaacctt gagtacctat
     1861 ctattataat aaacaagtat gttttataat tattttgatc ttgatatact tggatgatgg
     1921 catatgcagc agctatatgt ggattttttt agccctgcct tcatacgcta tttatttgct
     1981 tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg tgttacttct gcagcctgca
     2041 ggatggctcc taagaagaag cggaaggttg gtattcacgg ggtgcctgcg gcttcaaagc
     2101 tcgagaaatt caccaactgt tattcgttga gcaaaacact gcggtttaaa gcgattccag
     2161 tcggcaagac tcaagagaat atagacaata agcggctgtt ggtggaagat gaaaagcgcg
     2221 cggaagacta caaaggggtg aagaagttgt tggacagata ctacctctct tttatcaatg
     2281 atgtcttgca ctcaatcaaa ttgaagaatc tgaacaacta catctccctc ttcagaaaga
     2341 aaacaaggac agaaaaggag aataaggaac ttgaaaattt ggagatcaat ctgaggaaag
     2401 agatcgcgaa agcctttaaa ggcaacgaag gatacaaaag tctgttcaag aaggatataa
     2461 ttgagacaat tttgccagag ttcctcgatg acaaggacga gattgcgctg gtcaattcgt
     2521 tcaacggatt cacaacagca ttcacaggct tctttgataa tcgggaaaat atgttctctg
     2581 aggaggcaaa gtccacttct attgcgttca ggtgtatcaa tgagaatctc actaggtaca
     2641 tttccaacat ggatatcttt gagaaggttg acgcaatttt tgacaagcac gaagttcagg
     2701 agattaagga gaagatcctc aattccgatt atgacgttga ggacttcttc gaaggtgagt
     2761 tttttaattt cgtgctcact caagagggta tcgacgtgta taatgcgatc atcggtgggt
     2821 tcgtgactga gtccggtgaa aagattaagg gattgaacga gtatatcaac ctttacaacc
     2881 aaaagacgaa acagaagctg ccaaagttca agcctcttta caaacaggtt ctttcagacc
     2941 gcgagtcact ctcgttctat ggggagggct acacttcgga tgaggaagtc ctggaggtgt
     3001 tcaggaatac tctcaataag aattcggaga ttttctcttc tataaaaaaa ctggaaaagt
     3061 tgtttaagaa ttttgacgaa tactctagcg ccggcatatt tgtgaaaaac ggcccggcca
     3121 tatcaacgat aagtaaagat atcttcggcg aatggaacgt gatcagagac aaatggaacg
     3181 cggagtatga cgatattcac ctgaagaaga aggctgtcgt aacggagaag tacgaggatg
     3241 atcgcaggaa aagcttcaaa aagatcggaa gtttcagcct ggaacagttg caggagtatg
     3301 ctgacgccga tcttagcgtc gtcgagaagt tgaaggagat aatcatccaa aaggtcgatg
     3361 agatatataa agtctatgga tcaagtgaaa aactgttcga cgccgacttc gttttggaga
     3421 agtccctgaa gaagaacgac gctgttgttg ccattatgaa ggatctgctc gacagcgtga
     3481 agagtttcga gaactatatt aaggcttttt tcggggaggg gaaggagact aacagagatg
     3541 agtccttcta cggagacttc gtcctcgcgt acgatatact ccttaaggta gaccacatct
     3601 acgacgcaat cagaaattac gtgacacaaa agccgtacag caaggacaag ttcaaactct
     3661 acttccagaa cccccagttc atgggcggct gggacaagga caaggaaacg gattacaggg
     3721 ctacgatcct gaggtatggt tcaaaatact acttggcgat tatggacaag aagtacgcca
     3781 agtgtctcca gaagattgac aaagacgatg tcaatggcaa ttatgagaag atcaactaca
     3841 agctgcttcc gggtccgaac aagatgctcc caaaggtttt cttcagcaag aaatggatgg
     3901 cctactataa cccaagcgag gacatccaga agatttataa gaacggtacg ttcaagaagg
     3961 gcgacatgtt caatcttaac gactgtcaca agctgatcga cttcttcaaa gactcaatta
     4021 gccggtaccc aaagtggtct aacgcctatg acttcaactt ttcggaaacc gagaagtaca
     4081 aggatatagc cggattttat agagaggtgg aagagcaggg ctacaaggtg tcattcgagt
     4141 ccgccagcaa gaaggaagtg gacaagctcg tggaagaggg taagctctac atgttccaga
     4201 tttataataa agactttagc gataagagcc acgggacacc taatctccac acaatgtatt
     4261 tcaagctgct cttcgacgag aataaccacg gccaaatcag gttgtcagga ggggctgaac
     4321 tcttcatgcg gcgcgcatcc ctgaagaagg aggagcttgt agtccaccct gcgaatagtc
     4381 caattgcgaa taagaacccg gacaatccta aaaagactac aacattgagc tacgacgtgt
     4441 acaaggataa gaggttttcc gaggatcagt acgagctcca catcccgatt gcgatcaaca
     4501 agtgcccaaa gaatattttc aagataaaca cagaggtgcg tgtactcctg aagcatgacg
     4561 acaatcctta cgtcattggg attgatcggg gcgagaggaa cctcctctat attgtggtgg
     4621 tggacgggaa ggggaacata gtcgaacagt actcccttaa cgaaataatt aacaatttca
     4681 acggcatccg tatcaagacc gactaccatt cgttgctgga caagaaggag aaggagagat
     4741 ttgaggcgcg gcaaaattgg acaagtatcg agaacatcaa ggaactcaaa gcaggttata
     4801 tctctcaagt tgtgcataag atatgcgagc tggttgagaa gtatgacgca gtgatcgctc
     4861 ttgaggacct caactcgggc tttaagaata gccgcgttaa agtggagaag caggtctatc
     4921 aaaagttcga gaagatgctt atagataagc tcaactacat ggtcgataag aaatcgaacc
     4981 catgtgccac cggcggcgca ctcaaaggtt accaaataac aaacaaattc gagtccttca
     5041 aatcgatgag tactcagaat gggttcatat tttatatacc ggcgtggctt acgtctaaga
     5101 tcgacccgtc aactggtttt gtcaacctgt tgaagacgaa atacacgtcc attgccgatt
     5161 cgaaaaagtt catatctagt tttgatcgta ttatgtacgt cccagaggaa gatcttttcg
     5221 agtttgctct cgactacaaa aacttttcgc ggaccgatgc ggattacatt aaaaaatgga
     5281 aactctattc gtacggcaac agaatcagga tttttcgcaa ccctaagaag aataacgtct
     5341 ttgattggga ggaagtttgc ttgactagcg cgtacaaggt caatatgctg tcaaaggcaa
     5401 aattactgat gagtccgtga ggacgaaacg agtaagctcg tctaatttct actaagtgta
     5461 gatagagacc ggggtctccg gccggcatgg tcccagcctc ctcgctggcg ccggctgggc
     5521 aacatgcttc ggcatggcga atgggacttt cttttctttt ccttttcgtt gcttgttttc
     5581 cccttctgat gttgtgtgtt gagtttattg tttggtttgc tggtagatcc agtatttgga
     5641 tcatttttcc tttttttttt ctttcattgc ttctgcggag ggatgagttt gtgcatgaat
     5701 cttttgttgg ttggattcgc ttagttttca cactagtcct ttgattcagc gcaaatcttt
     5761 gttaattggc tcgcttaatt ctgacataaa gtgttccttc ttggttctgc ttgcaggagc
     5821 tctttaataa gtatggcatt aactaccaac agggtgatat cagagcactg ctttgcgaac
     5881 aatctgacaa ggctttctac tcatccttca tggctttgat gagcctgatg ctccagatga
     5941 gaaattcaat tacaggcaga accgacgtgg atttcttgat ctccccggtt aaaaattctg
     6001 atggcatctt ttacgatagc aggaactatg aagcgcaaga gaatgcgatt ctgccaaaaa
     6061 atgcagacgc caacggtgcc tataacatcg ccaggaaagt cctgtgggcg atcggccagt
     6121 tcaaaaaggc cgaagacgaa aaattggaca aggtcaaaat cgctatcagc aacaaagagt
     6181 ggctggagta tgctcagaca tccgtaaagc ataagcgtcc tgctgccacc aaaaaggccg
     6241 gacaggctaa gaaaaagaag tgatctagaa tatgaagatg aagatgaaat atttggtgtg
     6301 tcaaataaaa agcttgtgtg cttaagtttg tgtttttttc ttggcttgtt gtgttatgaa
     6361 tttgtggctt tttctaatat taaatgaatg taagatcaca ttataatgaa taaacaaatg
     6421 tttctataat ccattgtgaa tgttttgttg gatctcttct gcagcatata actactgtat
     6481 gtgctatggt atggactatg gaatatgatt aaagataagg agctcaaact atcagtgttt
     6541 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata
     6601 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag
     6661 ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag tgcagtcggc
     6721 ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg
     6781 cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata
     6841 aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct
     6901 ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc
     6961 gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc
     7021 gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg
     7081 accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc
     7141 caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg
     7201 gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc
     7261 atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc
     7321 cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc
     7381 cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca
     7441 cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag
     7501 gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa
     7561 gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg
     7621 cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacggctca accgtgcggc
     7681 tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg
     7741 ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct
     7801 tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga
     7861 acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc
     7921 aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga
     7981 tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt
     8041 cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt
     8101 agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga
     8161 cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt
     8221 ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt
     8281 gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta
     8341 cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc
     8401 cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc
     8461 gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc
     8521 aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg
     8581 ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag
     8641 gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta
     8701 tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat
     8761 tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga
     8821 atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgcctgccg
     8881 gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca
     8941 tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc
     9001 gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa
     9061 gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg
     9121 attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac
     9181 gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt
     9241 gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc
     9301 gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc
     9361 catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg
     9421 ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag
     9481 aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt
     9541 acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc
     9601 cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct
     9661 gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc
     9721 gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc
     9781 gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc
     9841 ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg
     9901 gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc
     9961 aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa
    10021 attgccctag caggggaaaa aggtcgaaaa ggcctctttc ctgtggatag cacgtacatt
    10081 gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac
    10141 attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc
    10201 gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg
    10261 tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc
    10321 ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc
    10381 ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg
    10441 cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
    10501 catgcagctc ccggaaacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
    10561 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
    10621 ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
    10681 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
    10741 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
    10801 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
    10861 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
    10921 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
    10981 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
    11041 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
    11101 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
    11161 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
    11221 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    11281 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    11341 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    11401 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    11461 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    11521 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc
    11581 attctaggta ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag
    11641 gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc
    11701 tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa
    11761 gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc
    11821 cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc
    11881 gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt
    11941 tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat
    12001 ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt
    12061 cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga
    12121 gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc
    12181 cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc
    12241 ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta tataccttag
    12301 caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt
    12361 ccggtgatat tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa
    12421 gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta
    12481 aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat
    12541 agtatcgacg gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg
    12601 ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt
    12661 gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc
    12721 ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc
    12781 tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg
    12841 acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa
    12901 ttaattcggg ggatctggat tttagtactg gattttggtt ttaggaatta gaaattttat
    12961 tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat
    13021 gagcgaaacc ctataggaac cctaattccc ttatctggga actactcaca cattattatg
    13081 gagaaaatag agcttgtcga tcgttaatta atcacctcca agatcccgga cgagtgctgg
    13141 ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag acggccgcgc
    13201 ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg attgcgtcgc
    13261 atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc tgatagagtt
    13321 ggtcaagacc aatgcggagc atatacgccc ggagccgcgg cgatcctgca agctccggat
    13381 gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc ctccagaaga
    13441 agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg
    13501 ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt
    13561 gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg
    13621 acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga tcagcaatcg
    13681 cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg aatgggccga
    13741 acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catggcctcc gcgaccggct
    13801 gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc
    13861 gggagatgca ataggtcagg ctctcgctga attccccaat gtcaagcact tccggaatcg
    13921 ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa ccatcggcgc
    13981 agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa gcacgagatt
    14041 cttcgccctc cgagagctgc atcaggtcgg acacgctgtc gaacttttcg atcagaaact
    14101 tctcgacaga cgtcgcggtg agttcaggct ttttcatcct aggagagata gatttgtaga
    14161 gagagactgg tgatttcagc gtgtcctctc caaatgaaat gaacttcctt atatagagga
    14221 agggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca
    14281 tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt
    14341 gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc
    14401 tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa
    14461 gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa
    14521 agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag
    14581 agtgtcgtgc tccaccatgt tcacatcaat ccacttgctt tgaagacgtg gttggaacgt
    14641 cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag
    14701 aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt
    14761 ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt
    14821 ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat
    14881 ctttgatatt cttggagtag acgagagtgt cgtgctccac catg
//
