LOCUS       sequence-272874-        7515 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses UL87-ORF24 chimera with a C-terminal 2xStrep tag in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7515)
  AUTHORS   Castaneda AF, Didychuk AL, Louder RK, McCollum CO, Davis ZH, Nogales
            E, Glaunsinger BA
  TITLE     The gammaherpesviral TATA-box-binding protein directly interacts 
            with the CTD of host RNA Pol II to direct late gene transcription.
  JOURNAL   PLoS Pathog. 2020 Sep 4;16(9):e1008843. doi: 
            10.1371/journal.ppat.1008843. eCollection 2020 Sep.
  PUBMED    32886723
REFERENCE   2  (bases 1 to 7515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2020-09-4"; volume: "16(9):e1008843. doi"; pages: "
            10.1371/journal.ppat.1008843. eCollection 2020 Sep"
COMMENT     Sequence Label: pcDNA4/TO-UL87-ORF24-2xStrep
FEATURES             Location/Qualifiers
     source          1..7515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3180..3203
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3240..3263
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(3289..3306)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3295..3519
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3565..3993
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3652..3671)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3862..3883
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4002..4022)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4007..4336
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4187..4322
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4249..4268
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4384..4431
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4450..4824
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4954..5075
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4991..5010)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5045..5064
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5124..5140)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5124..5140)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5137..5159)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5148..5164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5172..5178,5179..5196,5197..5202))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5217..5238
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5355..5372)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5526..6114)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5606..5625)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6285..7076,7077..7145))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6908..6927
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7146..7250)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7325..7344)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg gtaccgagct cggatccatg gccggcgctg
      781 cgccgcgccg cctcggctgc gacgctctaa tagtcgtcgg cggctccgct acgccgcgcc
      841 gggttttaca cgtccccgtg cacgttcgcg cctgcaacct cacccaagag ctatcgacgg
      901 gcgaggacgc ccgcttctgt cgtccgcgac ccgttaacgt cgaacgggta cgcgctgttt
      961 tcgcggctct ctaccgtgcc tgtcccgtac acgcgaggac cgagtccgag cgtgtcaagc
     1021 tggtactggg tcgtctgttg ctgggacccg tggccgtacc ctgtttttgc gacggtgaag
     1081 tggagggcca cggcgagcat ctggtaccca cgacgcagtt ttgtcgcggg ccgctgctct
     1141 acgtgcaccg acgttgttgt tgcggatccg tgaccgccgg gcgcgcgctg tcctaccacg
     1201 ttctcgaaaa ccacgtggcc acgcatgtgt tacgcggatt gctctcgctg acggaatgga
     1261 atcgagagtt gccgggcctt ttttgcgact gtcctggcag cggtggcgcc ttgggaaccg
     1321 aggaacgcta cgccatggcc tgcttgccgc gcgacctcag cctgcacctg gacgactatc
     1381 cttacctgat ggtggaaatc ggacgcgtac tcagcgtcag cgaggtagac gactacgtaa
     1441 ccgccgtctc cggctacctg ggcgaggccg cggcgccgcg cattcaggtt cactacaagc
     1501 tgctctttgg aagcctgaag catctctcgt tttcaataat gccacccgca tccccagacg
     1561 cacagctaca ttttttaaag tttaacatcg gcagcttcat ggccacgtgg gggtggcacg
     1621 gagagctggt ctcgctgcgc cgtgccatcg ctcacaacgt agagcgactg cccaccgtgc
     1681 tgaagaacct gtcgaaacag agtaagcacc aggacgtcaa ggttaacgga cgggatctgg
     1741 tgggctttca gctggctcta aaccagctcg tgtcccgtct gcacgtaaaa atccaacgca
     1801 aggaccccgg accaaagcca tacagggtgg tcgtcagtac cccagattgt acctactatc
     1861 tagtgtatcc gggcacaccg gccatctaca gactcgtcat gtgtatggca gtggcagact
     1921 gcatcggcca ctcgtgcagc ggactgcacc cctgcgcaaa ctttttaggc acccacgaga
     1981 caccgcgtct cctggcggcg acgctttcaa gaatccggta cgcgccgaaa gaccggcgag
     2041 cagccatgaa gggaaatttg caggcgtgct tccaacgata cgcggccacg gacgcgcgga
     2101 ctctgggcag ctccacagtg tcagacatgc tggaacccac aaaacacgtc agtttggaaa
     2161 acttcaagat caccatattc aacaccaaca tggtgattaa cactaagata agctgccacg
     2221 ttcctaacac cctgcaaaag actattttaa acatccccag attgaccaac aattttgtta
     2281 tacgaaagta ctccgtaaag gaaccttctt ttaccataag cgtgtttttt tccgacaaca
     2341 tgtgtcaagg caccgcaata aacatcaaca tcagtgggga catgctgcac tttctcttcg
     2401 caatgggtac gctgaaatgc tttctgccaa tcaggcacat atttcctgta tcgatagcaa
     2461 attggaactc cacgttggac ctgcacggac tggaaaacca gtacatggtg agaatggggc
     2521 gaaaaaacgt attttggacc acaaactttc catctgtggt ctccagcaag gatgggctaa
     2581 acgtgtcctg gtttaaggcc gcgacagcca cgatttctaa agtgtacggg cagcctcttg
     2641 tggaacagat tcgccacgag ctggcgccca ttctcacgga ccagcacgcg cgcatcgacg
     2701 gaaacaaaaa tagaatattc tccctacttg agcacagaaa ccgttcccaa atacagacgc
     2761 tacacaaaag gttcctggag tgtctggtgg aatgctgttc gtttctcagg cttgacgtgg
     2821 cttgcattag gcgagccgcc gcccggggcc tgtttgactt ctcaaagaag ataatcagtc
     2881 acactaaaag caaacacgag tgcgcagtac tgggatataa aaagtgtaac ctaatcccga
     2941 aaatctatgc ccgaaacaag aagaccaggc tagacgagtt gggccgcaat gcaaacttca
     3001 tttcgttcgt cgccaccacg ggtcatcggt tcgccgctct aaagccacaa attgtccgtc
     3061 acgccattcg caaactaggc ctgcactggc gccaccgaac ggccgcgtcc aacgagcaga
     3121 caccgccagc cgatccccgc gtacgttgcg tccgtccgct gctcgaggga ggcggtggat
     3181 ggagccaccc ccagttcgag aagggcggcg gcagcggcgg cggcagcggc ggcggcagct
     3241 ggagccaccc ccagttcgag aaggggcccg tttaaacccg ctgatcagcc tcgactgtgc
     3301 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     3361 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     3421 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     3481 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     3541 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     3601 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     3661 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     3721 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     3781 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     3841 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     3901 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     3961 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     4021 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4081 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4141 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     4201 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     4261 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     4321 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     4381 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     4441 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
     4501 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
     4561 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
     4621 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
     4681 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
     4741 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
     4801 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
     4861 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     4921 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     4981 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     5041 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     5101 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     5161 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     5221 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     5281 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     5341 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     5401 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     5461 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     5521 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     5581 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     5641 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     5701 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     5761 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     5821 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     5881 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     5941 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     6001 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6061 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6121 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6181 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     6241 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     6301 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     6361 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     6421 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     6481 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     6541 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     6601 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     6661 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     6721 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     6781 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     6841 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     6901 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     6961 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7021 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7081 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7141 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7201 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     7261 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc
     7321 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
     7381 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
     7441 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
     7501 ccagatatac gcgtt
//
