LOCUS       sequence-27288-e         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Lian SL, Li S, Abadal GX, Pauley BA, Fritzler MJ, Chan EK
  TITLE     The C-terminal half of human Ago2 binds to multiple GW-rich regions 
            of GW182 and requires GW182 to mediate silencing.
  JOURNAL   RNA. 2009 May . 15(5):804-13.
  PUBMED    19324964
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            May . 15(5):804-13."
COMMENT     Sequence Label: GFP-D1a_W1-2(aa270-346)
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    51..75
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(334..358)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    395..529
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(419..438)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     473..492
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(645..661)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(645..661)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(658..680)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    669..685
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(693..699,700..717,718..723))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    738..759
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 aggcgggatc tctcggcatg gacgagctgt acaagtctag aggatcatca acaagtttgt
       61 acaaaaaagc aggctccaaa gatggccttc ggaatagcac tggacttggt tcccaaaaca
      121 agtttgtagt tggtagcagc agcaataatg tgggccatgg aagtagtact gggccatggg
      181 gtttttccca tggagccata ataagcacat gtcaggtctc tgtggatgct cctgaaagca
      241 aatctgaaag tagcaacaat agaatgaatg cttggggcac tgtaagttct tcatcaaatg
      301 gagggttaaa tccaagcact ttgaattcat gagacccagc tttcttgtac aaagtggttg
      361 attcgacctc gaggccttaa gtgactaggt acccgatcca gacatgataa gatacattga
      421 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
      481 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
      541 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
      601 aaacccaagc tgggaagctc agatctgagc ttggcgtaat catggtcata gctgtttcct
      661 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      721 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttggc gctcactgcc
      781 cgctctccat ctcgagaaac cctgtcgttg ccagctgcaa ttaatgaatc cggccaaccg
      841 ccgcggggga gagaggcggg tttggcgtat tgggcgcctc ttttccgctt tgtgtctcac
      901 atgagaactc tcctcgcgcg cctctggtct gtttcgcgct gtcgcgcgcg agagcggatc
      961 ac
//
