LOCUS       sequence-272880-        7344 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mu24-ORF24 chimera with a C-terminal 2xStrep tag in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7344)
  AUTHORS   Castaneda AF, Didychuk AL, Louder RK, McCollum CO, Davis ZH, Nogales
            E, Glaunsinger BA
  TITLE     The gammaherpesviral TATA-box-binding protein directly interacts 
            with the CTD of host RNA Pol II to direct late gene transcription.
  JOURNAL   PLoS Pathog. 2020 Sep 4;16(9):e1008843. doi: 
            10.1371/journal.ppat.1008843. eCollection 2020 Sep.
  PUBMED    32886723
REFERENCE   2  (bases 1 to 7344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2020-09-4"; volume: "16(9):e1008843. doi"; pages: "
            10.1371/journal.ppat.1008843. eCollection 2020 Sep"
COMMENT     Sequence Label: pcDNA4/TO-mu24-ORF24-2xStrep
FEATURES             Location/Qualifiers
     source          1..7344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    206..224
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    227..245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     255..279
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2629..2652
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2689..2712
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(2738..2755)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2744..2968
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3014..3442
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3101..3120)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3311..3332
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3451..3471)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3456..3785
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3636..3771
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3698..3717
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3833..3880
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3899..4273
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4403..4524
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4440..4459)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4494..4513
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4573..4589)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4573..4589)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4586..4608)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4597..4613
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4621..4627,4628..4645,4646..4651))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4666..4687
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4804..4821)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4975..5563)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5055..5074)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5734..6525,6526..6594))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6357..6376
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6595..6699)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6774..6793)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6965..7344
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctccct atcagtgata gagatctccc tatcagtgat
      241 agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
      301 tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct agcgtttaaa
      361 cttaagcttg gtaccgagct cggatccatg acaatattct taccagtatt ctgtgatttg
      421 ccagaagtat accaagattg tgaatatgat gagaatagcc aactgtctat cttattctgg
      481 atatcatcca atacatatga ggtagtagag aatgtacaac aatttaatat acttggtaaa
      541 gatttaacat tctgtttatg tagagcttta agaagactgc tcctgggcat aagactgtac
      601 ccaacctgca atactgagtc taacagatat gtggttaccg gggacagata tgtgggcaat
      661 ggtttggtgg ttagtcccac aggtaggcat attcagcgca tagatacctg cacctatgtt
      721 ccagtgatat acagctttga acaaactgac gcacattatg atggcatggg acctggaaaa
      781 ctgagggcct tgtatcatga gcaattattt tttaaaggaa ttgattattc ctttgtattt
      841 tccaccctca tcagattttt atctatgaaa cagatagatg agtgttacta ctcttttatc
      901 catactttgg aaccctgggc caggtctgct tgtaaaaaaa attacttgaa gcttactgag
      961 agcctgaagc atctctcgtt ttcaataatg ccacccgcat ccccagacgc acagctacat
     1021 tttttaaagt ttaacatcgg cagcttcatg gccacgtggg ggtggcacgg agagctggtc
     1081 tcgctgcgcc gtgccatcgc tcacaacgta gagcgactgc ccaccgtgct gaagaacctg
     1141 tcgaaacaga gtaagcacca ggacgtcaag gttaacggac gggatctggt gggctttcag
     1201 ctggctctaa accagctcgt gtcccgtctg cacgtaaaaa tccaacgcaa ggaccccgga
     1261 ccaaagccat acagggtggt cgtcagtacc ccagattgta cctactatct agtgtatccg
     1321 ggcacaccgg ccatctacag actcgtcatg tgtatggcag tggcagactg catcggccac
     1381 tcgtgcagcg gactgcaccc ctgcgcaaac tttttaggca cccacgagac accgcgtctc
     1441 ctggcggcga cgctttcaag aatccggtac gcgccgaaag accggcgagc agccatgaag
     1501 ggaaatttgc aggcgtgctt ccaacgatac gcggccacgg acgcgcggac tctgggcagc
     1561 tccacagtgt cagacatgct ggaacccaca aaacacgtca gtttggaaaa cttcaagatc
     1621 accatattca acaccaacat ggtgattaac actaagataa gctgccacgt tcctaacacc
     1681 ctgcaaaaga ctattttaaa catccccaga ttgaccaaca attttgttat acgaaagtac
     1741 tccgtaaagg aaccttcttt taccataagc gtgttttttt ccgacaacat gtgtcaaggc
     1801 accgcaataa acatcaacat cagtggggac atgctgcact ttctcttcgc aatgggtacg
     1861 ctgaaatgct ttctgccaat caggcacata tttcctgtat cgatagcaaa ttggaactcc
     1921 acgttggacc tgcacggact ggaaaaccag tacatggtga gaatggggcg aaaaaacgta
     1981 ttttggacca caaactttcc atctgtggtc tccagcaagg atgggctaaa cgtgtcctgg
     2041 tttaaggccg cgacagccac gatttctaaa gtgtacgggc agcctcttgt ggaacagatt
     2101 cgccacgagc tggcgcccat tctcacggac cagcacgcgc gcatcgacgg aaacaaaaat
     2161 agaatattct ccctacttga gcacagaaac cgttcccaaa tacagacgct acacaaaagg
     2221 ttcctggagt gtctggtgga atgctgttcg tttctcaggc ttgacgtggc ttgcattagg
     2281 cgagccgccg cccggggcct gtttgacttc tcaaagaaga taatcagtca cactaaaagc
     2341 aaacacgagt gcgcagtact gggatataaa aagtgtaacc taatcccgaa aatctatgcc
     2401 cgaaacaaga agaccaggct agacgagttg ggccgcaatg caaacttcat ttcgttcgtc
     2461 gccaccacgg gtcatcggtt cgccgctcta aagccacaaa ttgtccgtca cgccattcgc
     2521 aaactaggcc tgcactggcg ccaccgaacg gccgcgtcca acgagcagac accgccagcc
     2581 gatccccgcg tacgttgcgt ccgtccgctg ctcgagggag gcggtggatg gagccacccc
     2641 cagttcgaga agggcggcgg cagcggcggc ggcagcggcg gcggcagctg gagccacccc
     2701 cagttcgaga aggggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     2761 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     2821 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     2881 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     2941 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     3001 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     3061 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     3121 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     3181 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     3241 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     3301 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     3361 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     3421 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     3481 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3541 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3601 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3661 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3721 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     3781 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
     3841 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     3901 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
     3961 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
     4021 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
     4081 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
     4141 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
     4201 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
     4261 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
     4321 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     4381 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     4441 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     4501 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     4561 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     4621 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     4681 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     4741 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     4801 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     4861 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     4921 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4981 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5041 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5101 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5161 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5221 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5281 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5341 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5401 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5461 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5521 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5581 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5641 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5701 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     5761 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     5821 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     5881 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     5941 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     6001 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     6061 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     6121 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     6181 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     6241 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6301 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     6361 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     6421 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6481 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6541 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6601 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     6661 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     6721 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
     6781 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     6841 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
     6901 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
     6961 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     7021 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     7081 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     7141 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     7201 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     7261 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     7321 gtattagtca tcgctattac catg
//
