LOCUS       sequence-272885-       15183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  STU2.0 LbCas12a for plant genome editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15183)
  AUTHORS   Tang X, Ren Q, Yang L, Bao Y, Zhong Z, He Y, Liu S, Qi C, Liu B, 
            Wang Y, Sretenovic S, Zhang Y, Zheng X, Zhang T, Qi Y, Zhang Y
  TITLE     Single transcript unit CRISPR 2.0 systems for robust Cas9 and Cas12a
            mediated plant genome editing.
  JOURNAL   Plant Biotechnol J. 2019 Jul;17(7):1431-1445. doi: 
            10.1111/pbi.13068. Epub 2019 Jan 17.
  PUBMED    30582653
REFERENCE   2  (bases 1 to 15183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.13068";
            journalName: "Plant Biotechnol J"; date: "2019-07"; volume: "17"; 
            issue: "7"; pages: "1431-1445"
COMMENT     Sequence Label: pGEL032
FEATURES             Location/Qualifiers
     source          1..15183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2006
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             2021..2041
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2066..5746
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
     CDS             5747..5794
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(5855..6160)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(5957..5976)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     terminator      6530..6778
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    6799..6823
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8124..8753
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9182..10255
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10321..10515
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10751..10773)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10849..10989
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10904..10923
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11004..11021)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11175..11763)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11255..11274)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11850..12644)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    13069..13093
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    13171..13345
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(14435..15111)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(14478..14498)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa ggtgagcatt
       61 gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca
      121 gtttatctat ctttatacat atatttaaac tttactctac gaataatata atctatagta
      181 ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag
      241 gacaattgag tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt
      301 ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt attagtacat
      361 ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac atctatttta
      421 ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata
      481 atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga
      541 aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa
      601 cgccgtcgac gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag
      661 cgaagcagac ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc
      721 caccgttgga cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg
      781 agccggcacg gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc
      841 tttcccaccg ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc
      901 acaccctctt tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagaactccc
      961 ccaaatccac ccgtcggcac ctccgcttca aggtacgccg ctcgtcctcc cccccccccc
     1021 ctctctacct tctcaagatc ggcgttccgg tccatggtta gggcccggta gttctacttc
     1081 tgttcatgtt tgtgttagat ccgtgtttgt gttagatccg tgctactagc gttcgtacac
     1141 ggatgcgacc tgtacgtcag acacgttctg attgctaact tgccagtgtt tctctttggg
     1201 gaatcctggg atggctctag ccgttccgca gacgggatcg atttcatgat tttttttgtt
     1261 tcgttgcata gggtttggtt tgcccttttc ctttatttca atatatgccg tgcacttgtt
     1321 tgtcgggtca tcttttcatg cttttttttg tcttggttgt gatgatgtgg tctggttggg
     1381 cggtcgttca agatcggagt agaattaatt ctgtttcaaa ctacctggtg gatttattaa
     1441 ttttggatct gtatgtgtgt gccatacata ttcatagtta cgaattgaag atgatggatg
     1501 gaaatatcga tctaggatag gtatacatgt tgatgcgggt tttactgatg catatacaga
     1561 gatgcttttt gttcgcttgg ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt
     1621 caagatcgga gtagaatact gtttcaaact acctggtgta tttattaatt ttggaactgt
     1681 atgtgtgtgt catacatctt catagttacg agtttaagat ggatggaaat atcgatctag
     1741 gataggtata catgttgatg tgggttttac tgatgcatat acatgatggc atatgcagca
     1801 tctattcata tgctctaacc ttgagtacct atctattata ataaacaagt atgttttata
     1861 attattttga tcttgatata cttggatgat ggcatatgca gcagctatat gtggattttt
     1921 ttagccctgc cttcatacgc tatttatttg cttggtactg tttcttttgt cgatgctcac
     1981 cctgttgttt ggtgttactt ctgcagcctg caggatggct cctaagaaga agcggaaggt
     2041 tggtattcac ggggtgcctg cggcttcaaa gctcgagaaa ttcaccaact gttattcgtt
     2101 gagcaaaaca ctgcggttta aagcgattcc agtcggcaag actcaagaga atatagacaa
     2161 taagcggctg ttggtggaag atgaaaagcg cgcggaagac tacaaagggg tgaagaagtt
     2221 gttggacaga tactacctct cttttatcaa tgatgtcttg cactcaatca aattgaagaa
     2281 tctgaacaac tacatctccc tcttcagaaa gaaaacaagg acagaaaagg agaataagga
     2341 acttgaaaat ttggagatca atctgaggaa agagatcgcg aaagccttta aaggcaacga
     2401 aggatacaaa agtctgttca agaaggatat aattgagaca attttgccag agttcctcga
     2461 tgacaaggac gagattgcgc tggtcaattc gttcaacgga ttcacaacag cattcacagg
     2521 cttctttgat aatcgggaaa atatgttctc tgaggaggca aagtccactt ctattgcgtt
     2581 caggtgtatc aatgagaatc tcactaggta catttccaac atggatatct ttgagaaggt
     2641 tgacgcaatt tttgacaagc acgaagttca ggagattaag gagaagatcc tcaattccga
     2701 ttatgacgtt gaggacttct tcgaaggtga gttttttaat ttcgtgctca ctcaagaggg
     2761 tatcgacgtg tataatgcga tcatcggtgg gttcgtgact gagtccggtg aaaagattaa
     2821 gggattgaac gagtatatca acctttacaa ccaaaagacg aaacagaagc tgccaaagtt
     2881 caagcctctt tacaaacagg ttctttcaga ccgcgagtca ctctcgttct atggggaggg
     2941 ctacacttcg gatgaggaag tcctggaggt gttcaggaat actctcaata agaattcgga
     3001 gattttctct tctataaaaa aactggaaaa gttgtttaag aattttgacg aatactctag
     3061 cgccggcata tttgtgaaaa acggcccggc catatcaacg ataagtaaag atatcttcgg
     3121 cgaatggaac gtgatcagag acaaatggaa cgcggagtat gacgatattc acctgaagaa
     3181 gaaggctgtc gtaacggaga agtacgagga tgatcgcagg aaaagcttca aaaagatcgg
     3241 aagtttcagc ctggaacagt tgcaggagta tgctgacgcc gatcttagcg tcgtcgagaa
     3301 gttgaaggag ataatcatcc aaaaggtcga tgagatatat aaagtctatg gatcaagtga
     3361 aaaactgttc gacgccgact tcgttttgga gaagtccctg aagaagaacg acgctgttgt
     3421 tgccattatg aaggatctgc tcgacagcgt gaagagtttc gagaactata ttaaggcttt
     3481 tttcggggag gggaaggaga ctaacagaga tgagtccttc tacggagact tcgtcctcgc
     3541 gtacgatata ctccttaagg tagaccacat ctacgacgca atcagaaatt acgtgacaca
     3601 aaagccgtac agcaaggaca agttcaaact ctacttccag aacccccagt tcatgggcgg
     3661 ctgggacaag gacaaggaaa cggattacag ggctacgatc ctgaggtatg gttcaaaata
     3721 ctacttggcg attatggaca agaagtacgc caagtgtctc cagaagattg acaaagacga
     3781 tgtcaatggc aattatgaga agatcaacta caagctgctt ccgggtccga acaagatgct
     3841 cccaaaggtt ttcttcagca agaaatggat ggcctactat aacccaagcg aggacatcca
     3901 gaagatttat aagaacggta cgttcaagaa gggcgacatg ttcaatctta acgactgtca
     3961 caagctgatc gacttcttca aagactcaat tagccggtac ccaaagtggt ctaacgccta
     4021 tgacttcaac ttttcggaaa ccgagaagta caaggatata gccggatttt atagagaggt
     4081 ggaagagcag ggctacaagg tgtcattcga gtccgccagc aagaaggaag tggacaagct
     4141 cgtggaagag ggtaagctct acatgttcca gatttataat aaagacttta gcgataagag
     4201 ccacgggaca cctaatctcc acacaatgta tttcaagctg ctcttcgacg agaataacca
     4261 cggccaaatc aggttgtcag gaggggctga actcttcatg cggcgcgcat ccctgaagaa
     4321 ggaggagctt gtagtccacc ctgcgaatag tccaattgcg aataagaacc cggacaatcc
     4381 taaaaagact acaacattga gctacgacgt gtacaaggat aagaggtttt ccgaggatca
     4441 gtacgagctc cacatcccga ttgcgatcaa caagtgccca aagaatattt tcaagataaa
     4501 cacagaggtg cgtgtactcc tgaagcatga cgacaatcct tacgtcattg ggattgatcg
     4561 gggcgagagg aacctcctct atattgtggt ggtggacggg aaggggaaca tagtcgaaca
     4621 gtactccctt aacgaaataa ttaacaattt caacggcatc cgtatcaaga ccgactacca
     4681 ttcgttgctg gacaagaagg agaaggagag atttgaggcg cggcaaaatt ggacaagtat
     4741 cgagaacatc aaggaactca aagcaggtta tatctctcaa gttgtgcata agatatgcga
     4801 gctggttgag aagtatgacg cagtgatcgc tcttgaggac ctcaactcgg gctttaagaa
     4861 tagccgcgtt aaagtggaga agcaggtcta tcaaaagttc gagaagatgc ttatagataa
     4921 gctcaactac atggtcgata agaaatcgaa cccatgtgcc accggcggcg cactcaaagg
     4981 ttaccaaata acaaacaaat tcgagtcctt caaatcgatg agtactcaga atgggttcat
     5041 attttatata ccggcgtggc ttacgtctaa gatcgacccg tcaactggtt ttgtcaacct
     5101 gttgaagacg aaatacacgt ccattgccga ttcgaaaaag ttcatatcta gttttgatcg
     5161 tattatgtac gtcccagagg aagatctttt cgagtttgct ctcgactaca aaaacttttc
     5221 gcggaccgat gcggattaca ttaaaaaatg gaaactctat tcgtacggca acagaatcag
     5281 gatttttcgc aaccctaaga agaataacgt ctttgattgg gaggaagttt gcttgactag
     5341 cgcgtacaag gagctcttta ataagtatgg cattaactac caacagggtg atatcagagc
     5401 actgctttgc gaacaatctg acaaggcttt ctactcatcc ttcatggctt tgatgagcct
     5461 gatgctccag atgagaaatt caattacagg cagaaccgac gtggatttct tgatctcccc
     5521 ggttaaaaat tctgatggca tcttttacga tagcaggaac tatgaagcgc aagagaatgc
     5581 gattctgcca aaaaatgcag acgccaacgg tgcctataac atcgccagga aagtcctgtg
     5641 ggcgatcggc cagttcaaaa aggccgaaga cgaaaaattg gacaaggtca aaatcgctat
     5701 cagcaacaaa gagtggctgg agtatgctca gacatccgta aagcataagc gtcctgctgc
     5761 caccaaaaag gccggacagg ctaagaaaaa gaagtgaaaa aaaaaaaaaa aaaaaaaaaa
     5821 aaaaaaaaaa aaaaaaaaaa aaaaaaagga gaccttatat tccccagaac atcaggttaa
     5881 tggcgttttt gatgtcattt tcgcggtggc tgagatcagc cacttcttcc ccgataacgg
     5941 aaaccggcac actggccata tcggtggtca tcatgcgcca gctttcatcc ccgatatgca
     6001 ccaccgggta aagttcacgg gagactttat ctgacagcag acgtgcactg gccaggggga
     6061 tcaccatccg tcgcccgggc gtgtcaataa tatcactctg tacatccaca aacagacgat
     6121 aacggctctc tcttttatag gtgtaaacct taaactgcat ttcaccagcc cctgttctcg
     6181 tcagcaaaag agccgttcat ttcaataaac cgggcgacct cagccatccc ttcctgattt
     6241 tccgctttcc agcgttcggc acgcagacga cgggcttcat tctgcatggt tgtgcttacc
     6301 agaccggaga tattgacatc atatatgcct tgagcaactg atagctgtcg ctgtcaactg
     6361 tcactgtaat acgctgcttc atagcatacc tctttttgac atacttcggg tatacatatc
     6421 agtatatatt cttataccgc aaaaatcagc gcgcaaatac gcatactgtt atctggcttg
     6481 gtctcgaatt tctactaagt gtagatggta cccggccgga attctagaat atgaagatga
     6541 agatgaaata tttggtgtgt caaataaaaa gcttgtgtgc ttaagtttgt gtttttttct
     6601 tggcttgttg tgttatgaat ttgtggcttt ttctaatatt aaatgaatgt aagatcacat
     6661 tataatgaat aaacaaatgt ttctataatc cattgtgaat gttttgttgg atctcttctg
     6721 cagcatataa ctactgtatg tgctatggta tggactatgg aatatgatta aagataagga
     6781 gctcaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt
     6841 ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt
     6901 atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg
     6961 ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc
     7021 gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt
     7081 cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat
     7141 gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga
     7201 cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa
     7261 gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc
     7321 tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact
     7381 ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg
     7441 ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga
     7501 gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg
     7561 aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga
     7621 gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg
     7681 ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg
     7741 gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa
     7801 tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc
     7861 attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg
     7921 cacggctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg
     7981 gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt
     8041 gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat
     8101 aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt
     8161 caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg
     8221 ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag
     8281 atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca
     8341 tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt
     8401 ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat
     8461 gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc
     8521 tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg
     8581 ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga
     8641 gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg
     8701 ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg
     8761 aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg
     8821 cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa
     8881 ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa
     8941 gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg
     9001 aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac
     9061 caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa
     9121 gcggctgggt tgcctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg
     9181 cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct
     9241 ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg
     9301 ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc
     9361 ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt
     9421 cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt
     9481 tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga
     9541 cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct
     9601 ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg
     9661 agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg
     9721 agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac
     9781 gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga
     9841 gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta
     9901 catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga
     9961 cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta
    10021 ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta
    10081 cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat
    10141 cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat
    10201 cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac
    10261 ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gcctctttcc
    10321 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
    10381 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
    10441 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
    10501 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
    10561 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
    10621 ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc
    10681 gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg
    10741 acggtgaaaa cctctgacac atgcagctcc cggaaacggt cacagcttgt ctgtaagcgg
    10801 atgccgggag cagacaagcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
    10861 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt
    10921 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
    10981 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
    11041 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
    11101 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
    11161 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    11221 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    11281 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    11341 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    11401 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    11461 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    11521 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    11581 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
    11641 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    11701 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    11761 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    11821 taagggattt tggtcatgca ttctaggtac taaaacaatt catccagtaa aatataatat
    11881 tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt
    11941 tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata ccacttgtcc
    12001 gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt cacaaagatg
    12061 ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt ttccgtcttt
    12121 aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca gttttcgcaa
    12181 tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg gctgtctaag
    12241 ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat gcactccgca
    12301 tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga gcaaaggacg
    12361 ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa gtgcaggacc
    12421 tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg ttccacatca
    12481 taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc attttctccc
    12541 accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca gcggtatttt
    12601 tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta tttccttcct
    12661 cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt
    12721 cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt
    12781 caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg
    12841 cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt
    12901 caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg
    12961 ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag
    13021 tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata
    13081 ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat
    13141 gtactgaatt aacgccgaat taattcgggg gatctggatt ttagtactgg attttggttt
    13201 taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat actaagggtt
    13261 tcttatatgc tcaacacatg agcgaaaccc tataggaacc ctaattccct tatctgggaa
    13321 ctactcacac attattatgg agaaaataga gcttgtcgat cgttaattaa tcacctccaa
    13381 gatcccggac gagtgctggg gcgtcggttt ccactatcgg cgagtacttc tacacagcca
    13441 tcggtccaga cggccgcgct tctgcgggcg atttgtgtac gcccgacagt cccggctccg
    13501 gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa attgccgtca
    13561 accaagctct gatagagttg gtcaagacca atgcggagca tatacgcccg gagccgcggc
    13621 gatcctgcaa gctccggatg cctccgctcg aagtagcgcg tctgctgctc catacaagcc
    13681 aaccacggcc tccagaagaa gatgttggcg acctcgtatt gggaatcccc gaacatcgcc
    13741 tcgctccagt caatgaccgc tgttatgcgg ccattgtccg tcaggacatt gttggagccg
    13801 aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct cggcccaaag catcagctca
    13861 tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac
    13921 acatggggat cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg accgattcct
    13981 tgcggtccga atgggccgaa cccgctcgtc tggctaagat cggccgcagc gatcgcatcc
    14041 atggcctccg cgaccggctg cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac
    14101 gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg
    14161 tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac ataacgatct
    14221 ttgtagaaac catcggcgca gctatttacc cgcaggacat atccacgccc tcctacatcg
    14281 aagctgaaag cacgagattc ttcgccctcc gagagctgca tcaggtcgga cacgctgtcg
    14341 aacttttcga tcagaaactt ctcgacagac gtcgcggtga gttcaggctt tttcatccta
    14401 ggagagatag atttgtagag agagactggt gatttcagcg tgtcctctcc aaatgaaatg
    14461 aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta
    14521 cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt
    14581 tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat
    14641 cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt
    14701 ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg
    14761 atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga
    14821 tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt cacatcaatc cacttgcttt
    14881 gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct
    14941 ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg
    15001 catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg
    15061 caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt
    15121 tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc
    15181 atg
//
