LOCUS       sequence-272887-        7670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus expression of CENP-HIKM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7670)
  AUTHORS   McKinley KL, Sekulic N, Guo LY, Tsinman T, Black BE, Cheeseman IM
  TITLE     The CENP-L-N Complex Forms a Critical Node in an Integrated Meshwork
            of Interactions at the Centromere-Kinetochore Interface.
  JOURNAL   Mol Cell. 2015 Dec 17;60(6):886-98. doi: 
            10.1016/j.molcel.2015.10.027. Epub 2015 Nov 19.
  PUBMED    26698661
REFERENCE   2  (bases 1 to 7670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.10.027"; journalName: "Mol Cell"; date: 
            "2015-12-17- 17"; volume: "60"; issue: "6"; pages: "886-98"
COMMENT     Sequence Label: pKM634
FEATURES             Location/Qualifiers
     source          1..7670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        52..143
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     promoter        4373..4464
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     regulatory      4945..4954
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5168..5302
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5205..5224)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5259..5278
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5423..5588
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      5778..6366
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6267..6286
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  6501..6724
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(6791..7324)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(7513..7518,7519..7535,7536..7541))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(7612..7645)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gcgagtttaa acactagtat cgattcgcga cctactccgg aatattaata gatcatggag
       61 ataattaaaa tgataaccat ctcgcaaata aataagtatt ttactgtttt cgtaacagtt
      121 ttgtaataaa aaaacctata aatattccgg attattcata ccgtcccacc atcgggcgcg
      181 gatccatgga ggaacagcca caaatgcagg atgccgatga accagcagac tcaggaggtg
      241 aaggtcgcgc aggaggcccg ccacaagttg ccggagcaca agcagcgtgt agtgaggatc
      301 gtatgaccct gctgctgcgc ctgcgtgcac aaacaaaaca gcaactgctg gagtataaaa
      361 gcatggtcga tgccagcgaa gaaaaaaccc cagagcaaat catgcaagag aaacaaatcg
      421 aggccaaaat cgaggacctg gaaaacgaaa ttgaagaagt gaaagtcgcc tttgagatca
      481 aaaaactggc cctggatcgt atgcgtctgt ctacagccct gaaaaaaaac ctggagaaaa
      541 tcagtcgtca gtcaagcgtt ctgatggaca acatgaaaca cctgctggaa ctgaacaaac
      601 tgatcatgaa atctcagcaa gagtcgtggg atctggagga gaaactgctg gatattcgta
      661 aaaaacgcct gcagctgaaa caagcaagcg agtctaaact gctcgagatc cagaccgaaa
      721 aaaacaaaca gaaaatcgac ctggacagca tggaaaattc cgagcgtatc aaaatcatcc
      781 gccagaatct gcagatggaa atcaaaatca ccaccgtcat ccaacacgtc tttcagaacc
      841 tgatcctggg tagcaaagtg aattgggccg aggaccctgc cctgaaagag attgttctgc
      901 aactggagaa aaatgtggac atgatgtgag tcgacgagmt caykkrkmkm rttaaaatga
      961 taaccatctc gcaaataaat aagtatttta ctgttttcgt aacagttttg taataaaaaa
     1021 acctataaat attccggatt attcataccg tcccaccatc gggcgcggat ccatgagccc
     1081 acagaaacgt gtaaaaaatg tccaggccca gaaccgtacc agtcaaggca gtagcagctt
     1141 ccaaacaacc ctgtcagcat ggaaagtgaa acaggacccg agcaacagca aaaacatctc
     1201 taaacatggc cagaataacc cagttggcga ctatgagcac gccgatgatc aagcagaaga
     1261 ggacgccctg caaatggcgg taggctattt cgaaaaaggc cctatcaaag ccagtcagaa
     1321 caaagacaaa acgctggaga aacacctgaa aaccgtggag aacgtggcat ggaaaaatgg
     1381 tctggcgagc gaagaaatcg acatcctgct gaatattgcc ctgagcggca aatttggtaa
     1441 tgccgtgaat acccgtatcc tgaaatgtat gatcccggca accgtgatta gtgaagattc
     1501 cgtcgtgaaa gcggtgtcat ggctgtgtgt tggcaaatgt tccggtagca cgaaagttct
     1561 gttctatcgc tggctggttg ccatgttcga ttttatcgac cgtaaagagc agatcaacct
     1621 gctgtatggc ttcttctttg cctcactgca ggacgatgcc ctgtgtcctt atgtgtgtca
     1681 cctgctgtat ctgctgacca aaaaagaaaa cgtcaaacct ttccgtgtcc gtaaactgct
     1741 ggacctgcaa gccaaaatgg gtatgcagcc tcacctgcaa gcactgctgt ctctgtataa
     1801 attctttgcc ccggcactga tttccgttag tctgccggtt cgtaaaaaaa tctatttcaa
     1861 aaacagcgag aacctgtgga aaacggctct gctggccgtt aaacagcgta atcgtggtcc
     1921 ttcccctgaa ccgctgaaac tgatgctggg accggctaac gttcgtccgc tgaaacgtaa
     1981 atggaactcc ctgtcggtga ttccagtact gaactcaagc tcgtatacta aagagtgtgg
     2041 taaaaaagag atgagcctga gcgattgtct gaatcgtagt ggctccttcc ctctggagca
     2101 gctgcaatct ttcccacaac tgctgcaaaa catccattgt ctggaactgc ctagccaaat
     2161 gggctctgtg ctgaacaatt cgctgctgct gcactatatc aactgtgtcc gtgatgaacc
     2221 agttctgctg cgcttctatt attggctgtc tcagactctg caggaagagt gtatctggta
     2281 taaagtgaac aactatgagc gtggtaaaga gttcactaac tttctcgaca ccattattcg
     2341 tgccgaatgc tttctgcaag agggcttcta ttcctgtgaa gcgttcctgt ataaaagcct
     2401 gccactgtgg gatgggctgt gttgtcgtag ccagtttctg caactggtga gctggattcc
     2461 ttttagcagc tttagcgagg ttaaaccgct gctgtttgac catctggcac agctgttctt
     2521 caccagcacc atctatttca aatgctccgt gctgcaatcc ctgaaagagc tgctgcaaaa
     2581 ttggctgctg tggctgtcta tggatattca catgaaaccg gtgaccaatt ctcctctgga
     2641 aaccactctg ggtggttcta tgaatagcgt gagcaaactg atccactatg tgggttggct
     2701 gagtacaacc gcaatgcgtc tggagagcaa caacaccttt ctgctgcatt tcatcctgga
     2761 cttctatgag aaagtgtgtg acatctatat caattataac ctgcctctgg ttgtgctgtt
     2821 ccctcctggc atcttctata gcgccctgct gtctctggat actagcatcc tgaatcaact
     2881 gtgcttcatt atgcaccgtt atcgtaaaaa cctgacggcg gcgaaaaaaa acgagctggt
     2941 acagaaaacc aaaagcgagt tcaacttcag ctccaaaacc tatcaagagt ttaatcatta
     3001 tctgacctcc atggtcgggt gtctgtggac atcaaaaccg ttcggtaaag gcatctatat
     3061 cgacccggag atcctggaaa aaacgggtgt agccgagtat aaaaactccc tgaacgtggt
     3121 ccaccatccg tcatttctgt cttatgctgt gtccttcctg ctgcaggaat ctcctgaaga
     3181 gcgtaccgtt aatgtgagca gtattcgtgg caaaaaatgg tcgtggtatc tggactatct
     3241 gttctctcaa ggtctgcaag ggctgaaact gtttatccgt agcagcgttc accatagcag
     3301 tatccctcgt gctgaaggga tcaactgtaa taaccagtat ctggagtgag aattcaaagg
     3361 ymwwmgwysa ygrrswyamt trwmrygrta accatctcgc aaataaataa gtattttact
     3421 gttttcgtaa cagttttgta ataaaaaaac ctataaatat tccggattat tcataccgtc
     3481 ccaccatcgg gcgcggatcc atgaaccagg aggatctgga ccctgatagt actaccgatg
     3541 tcggtgatgt gaccaatacc gaagaagagc tgattcgtga gtgtgaagaa atgtggaaag
     3601 acatggagga atgccaaaac aaactgagcc tgattggaac cgaaactctg accgatagca
     3661 atgcccaact gagtctgctg attatgcagg tgaaatgcct gactgctgaa ctgagccaat
     3721 ggcagaaaaa aacaccggaa accatcccgc tgactgaaga tgtgctgatt acgctgggta
     3781 aagaagaatt tcagaaactg cgccaggacc tggaaatggt gctgtctacc aaagagtcca
     3841 aaaacgaaaa actgaaagag gacctggagc gtgaacaacg ttggctggac gaacagcagc
     3901 aaatcatgga atccctgaac gttctgcact ctgagctgaa aaacaaagtg gaaaccttta
     3961 gcgaaagccg tatctttaac gaactgaaaa caaaaatgct gaacatcaaa gagtataaag
     4021 agaaactgct gagtacactg ggggaatttc tggaagatca cttccctctg cctgatcgta
     4081 gtgttaaaaa aaaaaaaaaa aacatccaag aaagctccgt gaacctgatc actctgcacg
     4141 agatgctgga aatcctgatt aatcgcctgt ttgacgtgcc acacgaccct tatgtgaaaa
     4201 tctccgacag cttctggcct ccttatgttg aactgctgct gcgtaatggt awwgcwcwgy
     4261 kksmtscwgm saccgctgag caataactat cataacccct agggtatacc catctaaggt
     4321 agcgagttta aacactagta tcgattcgcg acctactccg gaatattaat agatcatgga
     4381 gataattaaa atgataacca tctcgcaaat aaataagtat tttactgttt tcgtaacagt
     4441 tttgtaataa aaaaacctat aaatattccg gattattcat accgtcccac catcgggcgc
     4501 ggatccaact cctaaaaaac cgccaccatg tcggtgttga ggcccctgga caagctgccc
     4561 ggcctgaaca cggccacaat cttgctggtg ggcacggagg atgctcttct gcagcagctg
     4621 gcggactcga tgctcaaaga ggactgcgcc tccgagctga aggtccactt ggcaaagtcc
     4681 ctccctttgc cctccagtgt gaatcggccc cgaattgacc tgatcgtgtt tgtggttaat
     4741 cttcacagca aatacagtct ccagaacaca gaggagtccc tgcgccatgt ggatgccagc
     4801 ttcttcttgg ggaaggtgtg tttcctcgcc acaggtgctg ggcgggagag ccactgcagc
     4861 attcaccggc acaccgtggt gaagctggcc cacacctatc aaagccccct gctctactgt
     4921 gacctggagg tggaaggctt tagggccacc atggcgcagc gcctggtgcg cgtgctgcag
     4981 atctgtgctg gccacgtgcc cggtgtctca gctctgaacc tgctsyyscw gyykmgamrm
     5041 kcttgtcgag aagtactaga ggatcataat cagccatacc acatttgtag aggttttact
     5101 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     5161 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     5221 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5281 tgtatcttat catgtctgga tctgatcact gcttgagcct agaagatccg gctgctaaca
     5341 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta tcataacccc
     5401 tagggtatac ccatctaatk ggaaccagat aagtgaaatc tagttccaaa ctattttgtc
     5461 atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat tttgtcactc
     5521 ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcccaa ctattttgtc
     5581 cgcccacaac cggttgactt gggtcaactg tcagaccaag tttactcata tatactttag
     5641 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5701 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5761 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5821 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5881 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     5941 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6001 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6061 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6121 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6181 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6241 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6301 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6361 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6421 cacatgttct ttcctgcgtt atcccctgat tgacttgggt cgctcttcct gtggatgcgc
     6481 agatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa caaaatagat
     6541 ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga aatcagtcca
     6601 gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact cttcattttc
     6661 tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg taaagactat
     6721 attcgcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg atctcggctt
     6781 gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact tcttcccgta
     6841 tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct tgcacgtaga
     6901 tcacataagc accaagcgcg ttggcctcat gcttgaggag attgatgagc gcggtggcaa
     6961 tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc tcactacgcg
     7021 gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc aacaaccgct tcttggtcga
     7081 aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa tcggagtccg
     7141 gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga tcaagagcag
     7201 cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg cccatacttg
     7261 agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctgcgta
     7321 acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg
     7381 gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa aaccgccact
     7441 gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc
     7501 tacttgcatt acagtttacg aaccgaacag gcttatgtca actgggttcg tgccttcatc
     7561 cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcgc cataacttcg
     7621 tatagcatac attatacgaa gttatctgta actataacgg tcctaaggta
//
